Positive effect of reduced aeration rate on secretion of alpha-amylase and neutral proteases during pressurised fermentation of thermophilic Bacillus caldolyticus.

Abstract:

:The thermophilic microorganism Bacillus caldolyticus was incubated in laboratory scale stirred bioreactors under pressurised conditions at different aeration rates. Increased amounts of CO2/bicarbonate were solubilised under the chosen conditions. A reduction in aeration rate from 1 vvm to 0.1 vvm resulted in accumulation of CO2 and bicarbonate up to 126 mg l(-1) and 733 mg l(-1), respectively and also increased secretion of α-amylase and neutral proteases (increases of 123% and 52%, respectively). In this paper, the effect of reduced aeration rate on CO2/bicarbonate concentration and enzyme activities is presented. The selected fermentation conditions are closely related to those prevalent in large scale bioreactors and may offer the possibility of achieving high enzyme yields at reduced aeration costs on an industrial scale.

journal_name

N Biotechnol

journal_title

New biotechnology

authors

Popović MK,Senz M,Bader J,Skelac L,Schilf W,Bajpai R

doi

10.1016/j.nbt.2013.10.007

subject

Has Abstract

pub_date

2014-03-25 00:00:00

pages

141-9

issue

2

eissn

1871-6784

issn

1876-4347

pii

S1871-6784(13)00136-2

journal_volume

31

pub_type

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