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Molecular characterization of constitutive heterochromatin in three species of Trypoxylon (Hymenoptera: Crabronidae: Trypoxylini) by CMA3/DAPI staining.

Abstract：

:Previous cytogenetic analyses in Trypoxylon Latreille, 1796 have been basically restricted to C-banding. In the present study, base-specific CMA3 and DAPI fluorochrome staining were used to characterize the constitutive heterochromatin in three Trypoxylon species. The heterochromatin was GC-rich in all the species studied; however, in Trypoxylon nitidum F. Smith, 1856the molecular composition of the heterochromatinwasdifferent among chromosome pairs. Conversely, the euchromatin was AT-rich in the three species. These results suggest high conservatism in the euchromatic regions as opposed to the heterochromatic regions that have a high rate of changes. In this study, we report the karyotype of Trypoxylon rugifrons F. Smith, 1873which has the lowest chromosome number in the genus and other characteristics of the likely ancestral Trypoxylon karyotype.

journal_name

Comp Cytogenet

journal_title

Comparative cytogenetics

authors

Menezes RS,Carvalho AF,Silva JG,Costa MA

doi

10.3897/compcytogen.v5i2.961

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

71-80

issue

2

eissn

1993-0771

issn

1993-078X

journal_volume

5

pub_type

杂志文章

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