当前位置： SCI文献检索 > Comparative Cytogenetics期刊下所有文献 > Karyotyping and in situ chromosomal localization of rDNA sites in black cumin Bunium persicum (Boiss) B. Fedtsch,1915 (Apiaceae).

Karyotyping and in situ chromosomal localization of rDNA sites in black cumin Bunium persicum (Boiss) B. Fedtsch,1915 (Apiaceae).

Abstract：

:The fluorescent in situ hybridization (FISH) technique has been applied to somatic chromosomes in the medicinally important species, Bunium persicum, to elucidate its karyotypes. The bicolour FISH technique involving 18S-5.8S-26S and 5S ribosomal RNA genes as probes was used to assign physical localization and measurement of rDNA sites on homologous pairs of chromosomes. The two 18S-5.8S-26S rRNA gene sites were at the terminal regions of the short arms of the chromosomes 1 and 2 involving NOR region of chromosome 1. The 5S rDNA sites were found on subtelomeric region of the long arm of the chromosome number 5 and at interstitial regions of the short arm of chromosome 7. Based on direct visual analysis of chromosome length, morphology and position of FISH signals, a pioneer attempt has been made to construct metaphase karyotype in Bunium persicum, an endangered medicinal plant of North Western Himalayas.

journal_name

Comp Cytogenet

journal_title

Comparative cytogenetics

authors

Chahota RK,Mukai Y,Chaudhary HK,Kishore N,Sharma TR

doi

10.3897/CompCytogen.v5i4.965

subject

Has Abstract

pub_date

2011-11-09 00:00:00

pages

345-53

issue

4

eissn

1993-0771

issn

1993-078X

journal_volume

5

pub_type

杂志文章

相关文献

Comparative Cytogenetics文献大全