Immunogenic nature of a Pol gene product of HTLV-III/LAV.

Abstract:

:The present studies were initiated to define the coding region of a 34 kilodalton (kd) protein (p34) frequently observed with antibodies from HTLV-III/LAV-infected people by immunoblotting and radioimmunoprecipitation (RIP) techniques. We have directly mapped this viral protein to the pol gene of HTLV-III/LAV by radiolabeled amino acid sequence analysis. This region at the 3' end of the pol gene is predicted to encode the endonuclease/integrase of the virus. The seroprevalence rate of antibodies to the pol gene products p64 and p53 and to the endonuclease p34 were evaluated. Of 161 HTLV-III/LAV seropositive people tested by immunoblotting procedures, greater than 98% had antibodies which reacted to p64/p53 and 92.6% reacted to p34 indicating that these viral proteins are highly immunogenic in nature. We have also analyzed the serum of nine healthy people living in West Africa who were infected with HTLV-IV, a closely related retrovirus. Nine of nine seropositive people had antibodies that cross-reacted to p34 of HTLV-III/LAV, whereas only seven of nine reacted to p64/p53. These studies and our earlier observations indicate that current diagnostic procedures for screening for HTLV-III/LAV infection may also detect HTLV-IV seropositive individuals, pointing to a need for more specific assay systems.

journal_name

Blood

journal_title

Blood

authors

Allan JS,Coligan JE,Lee TH,Barin F,Kanki PJ,M'Boup S,McLane MF,Groopman JE,Essex M

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

331-3

issue

1

eissn

0006-4971

issn

1528-0020

journal_volume

69

pub_type

杂志文章

相关文献

BLOOD文献大全
  • Identification of primary lysosomes in human megakaryocytes and platelets.

    abstract::The presence of lysosomal enzymes in human platelets is well documented; the identity of the "lysosome," however, has been the subject of some disagreement. In order to determine the time of appearance and subcellular localization of two lysosomal enzymes in megakaryocytes (MK) and platelets, we examined normal human ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Bentfeld-Barker ME,Bainton DF

    更新日期:1982-03-01 00:00:00

  • Vesicular anthracycline accumulation in doxorubicin-selected U-937 cells: participation of lysosomes.

    abstract::The U-A10 cell line, a doxorubicin-selected variant of human U-937 myeloid leukemia cells, exhibits a redistribution of anthracyclines into a expanded vesicular compartment. The acidic nature of this compartment was confirmed by vital staining with a pH sensitive dye, LysoSensor yellow/blue DND-160. Identification of ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Hurwitz SJ,Terashima M,Mizunuma N,Slapak CA

    更新日期:1997-05-15 00:00:00

  • Human neutrophils release the Leu-8 lymph node homing receptor during cell activation.

    abstract::The Leu-8 molecule, the human homologue of the murine MEL-14 peripheral lymph node homing receptor, is expressed on neutrophils in both species and may be important in localization of cells to sites of inflammation. Most circulating human neutrophils express the Leu-8 molecule, and activation of neutrophils with phorb...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Berg M,James SP

    更新日期:1990-12-01 00:00:00

  • Remodeling of the malaria parasite and host human red cell by vesicle amplification that induces artemisinin resistance.

    abstract::Artemisinin resistance threatens worldwide malaria control and elimination. Elevation of phosphatidylinositol-3-phosphate (PI3P) can induce resistance in blood stages of Plasmodium falciparum The parasite unfolded protein response (UPR) has also been implicated as a proteostatic mechanism that may diminish artemisinin...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2017-11-814665

    authors: Bhattacharjee S,Coppens I,Mbengue A,Suresh N,Ghorbal M,Slouka Z,Safeukui I,Tang HY,Speicher DW,Stahelin RV,Mohandas N,Haldar K

    更新日期:2018-03-15 00:00:00

  • Deformability measurements on individual sickle cells using a new system with pO2 and temperature control.

    abstract::Although the rheologic behavior of sickle erythrocytes (SS cells) is highly dependent on oxygen tension (pO2) and temperature, very little data exist regarding the effects of deoxygenation and reoxygenation on the rheology of "individual" SS cells at body temperature. We have devised and assessed a new experiment syst...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Itoh T,Chien S,Usami S

    更新日期:1992-04-15 00:00:00

  • The VEGF-regulated transcription factor HLX controls the expression of guidance cues and negatively regulates sprouting of endothelial cells.

    abstract::The HLX gene encoding a diverged homeobox transcription factor has been found to be up-regulated by vascular endothelial growth factor-A (VEGF-A) in endothelial cells. We have now investigated the gene repertoire induced by HLX and its potential biologic function. HLX strongly increased the transcripts for several rep...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2010-07-293209

    authors: Testori J,Schweighofer B,Helfrich I,Sturtzel C,Lipnik K,Gesierich S,Nasarre P,Hofer-Warbinek R,Bilban M,Augustin HG,Hofer E

    更新日期:2011-03-03 00:00:00

  • Safety of autologous, ex vivo-expanded human immunodeficiency virus (HIV)-specific cytotoxic T-lymphocyte infusion in HIV-infected patients.

    abstract::We infused six human immunodeficiency virus (HIV)-seropositive subjects with autologous CD8+ cytotoxic T cells (CTLs) enriched for HIV-specific cytotoxicity targeted against a diversity of HIV epitopes in gp120, gag p17 and p24, and nef. There was no toxicity and no subject deteriorated clinically. In the first 2 week...

    journal_title:Blood

    pub_type: 临床试验,杂志文章

    doi:

    authors: Lieberman J,Skolnik PR,Parkerson GR 3rd,Fabry JA,Landry B,Bethel J,Kagan J

    更新日期:1997-09-15 00:00:00

  • Production of laminin B2 chain protein and messenger RNA by a murine neutrophil precursor cell line, 32Dc13.

    abstract::Laminin is a heterotrimeric glycoprotein that plays a central role in promoting neutrophil chemotaxis, motility, and attachment to basement membrane. Rabbit peritoneal exudate neutrophils stain positively for laminin, which is presumed to be of exogenous origin and bound to laminin receptors on the cell surface. We ex...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Tweardy DJ,Sasaki M,Cardamone JJ Jr,McCoy JP Jr,Bonidie MJ,Signorella AP

    更新日期:1990-10-01 00:00:00

  • Reduced-intensity conditioning for unrelated donor hematopoietic stem cell transplantation as treatment for myeloid malignancies in patients older than 55 years.

    abstract::Hematopoietic stem cell transplantation from unrelated donors is an effective treatment for myeloid malignancies, but its use is usually restricted to young patients without comorbidities. The development of reduced-intensity preparative regimens has allowed the extension of this form of treatment to older and medical...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-03-0855

    authors: Wong R,Giralt SA,Martin T,Couriel DR,Anagnostopoulos A,Hosing C,Andersson BS,Cano P,Shahjahan M,Ippoliti C,Estey EH,McMannis J,Gajewski JL,Champlin RE,de Lima M

    更新日期:2003-10-15 00:00:00

  • Reduction of the inhibitory antibody response to human factor VIII in hemophilia A mice by mutagenesis of the A2 domain B-cell epitope.

    abstract::Approximately 25% of patients with hemophilia A develop inhibitory antibodies after treatment with factor VIII. Most of the inhibitory activity is directed against epitopes in the A2 and C2 domains. Anti-A2 inhibitory antibodies recognize a 25-residue segment bounded by R484-I508. Several antigenic residues in this se...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-11-3891

    authors: Parker ET,Healey JF,Barrow RT,Craddock HN,Lollar P

    更新日期:2004-08-01 00:00:00

  • Lymphoid apoptosis and myeloid hyperplasia in CCAAT displacement protein mutant mice.

    abstract::CCAAT displacement protein (cux/CDP) is an atypical homeodomain protein that represses expression of several developmentally regulated lymphoid and myeloid genes in vitro, including gp91-phox, immunoglobulin heavy chain, the T-cell receptor beta and gamma chains, and CD8. To determine how this activity affects cell de...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v98.13.3658

    authors: Sinclair AM,Lee JA,Goldstein A,Xing D,Liu S,Ju R,Tucker PW,Neufeld EJ,Scheuermann RH

    更新日期:2001-12-15 00:00:00

  • Escape from suppression: tumor-specific effector cells outcompete regulatory T cells following stem-cell transplantation.

    abstract::Immune reconstitution of autologous hematopoietic stem-cell transplant recipients with the progeny of mature T cells in the graft leads to profound changes in the emerging functional T-cell repertoire. In the steady state, the host is frequently tolerant to tumor antigens, reflecting dominant suppression of naive and ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2007-06-096586

    authors: Mirmonsef P,Tan G,Zhou G,Morino T,Noonan K,Borrello I,Levitsky HI

    更新日期:2008-02-15 00:00:00

  • Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection.

    abstract::In human immunodeficiency virus (HIV)-1 infection, decrease of telomere length is mainly found in CD8(+) T cells and not in CD4(+) T cells. Telomerase, a ribonucleoprotein enzyme that can synthesize telomeric sequence onto chromosomal ends, can compensate for telomere loss. Here, we investigated if telomerase activity...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Wolthers KC,Otto SA,Wisman GB,Fleury S,Reiss P,ten Kate RW,van der Zee AG,Miedema F

    更新日期:1999-02-01 00:00:00

  • Molecular mechanisms of thrombus formation in ischemic stroke: novel insights and targets for treatment.

    abstract::In ischemic stroke, treatment options are limited. Therapeutic thrombolysis is restricted to the first few hours after stroke, and the utility of current platelet aggregation inhibitors, including GPIIb/IIIa receptor antagonists, and anticoagulants is counterbalanced by the risk of intracerebral bleeding complications...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2008-04-144758

    authors: Stoll G,Kleinschnitz C,Nieswandt B

    更新日期:2008-11-01 00:00:00

  • Inhibitory effect of the transcription factor encoded by the mutant mi microphthalmia allele on transactivation of mouse mast cell protease 7 gene.

    abstract::The transcription factor encoded by the mi locus (MITF) is a transcription factor of the basic-helix-loop-helix zipper protein family. Mice of mi/mi genotype express a normal amount of abnormal MITF, whereas mice of tg/tg genotype do not express any MITFs due to the transgene insertional mutation. The effect of normal...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v97.3.645

    authors: Ogihara H,Morii E,Kim DK,Oboki K,Kitamura Y

    更新日期:2001-02-01 00:00:00

  • Growth of human myeloid leukemias in the human marrow environment of SCID-hu mice.

    abstract::It has been shown previously that multilineage human hematopoiesis is maintained within human fetal bone marrow (BM) fragments implanted into severe combined immunodeficient (SCID) mice. We describe here an application of this animal model, the SCID-hu mouse, to the study of human myeloid leukemias. BM cells from 8 pa...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Namikawa R,Ueda R,Kyoizumi S

    更新日期:1993-10-15 00:00:00

  • Experimental Pseudomonas pneumonia in leukopenic dogs: comparison of therapy with antibiotics and granulocyte transfusions.

    abstract::Pseudomonas aeruginosa pneumonia was produced in dogs with radiation-induced leukopenia to study the comparative efficacy of several different therapies. In a randomized control trial, five treatment regimens were compared: no antibiotics or granulocytes (controls), gentamicin (5 mg/kg/day), carbenicillin (500 mg/kg/d...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Dale DC,Reynolds HY,Pennington JE,Elin RJ,Herzig GP

    更新日期:1976-05-01 00:00:00

  • The c-kit receptor ligand functions as a mast cell chemoattractant.

    abstract::Mast cells accumulate at sites of neovascularization, solid tumors, and many immune reactions. Such accumulation requires directed migration of mature mast cells or their precursors. The nature of the chemoattractants that regulate mast cell motility and the identity of the receptors that mediate the chemotactic respo...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Meininger CJ,Yano H,Rottapel R,Bernstein A,Zsebo KM,Zetter BR

    更新日期:1992-02-15 00:00:00

  • IFN-gamma gene polymorphisms associate with development of EBV+ lymphoproliferative disease in hu PBL-SCID mice.

    abstract::Posttransplantation lymphoproliferative disorder (PTLD) is a devastating post-transplantation complication often associated with Epstein-Barr virus (EBV). Although the type and length of immunosuppression are risk factors, a patient's inherent immune capacity also likely contributes to this disorder. This report uses ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-07-2476

    authors: Dierksheide JE,Baiocchi RA,Ferketich AK,Roychowdhury S,Pelletier RP,Eisenbeis CF,Caligiuri MA,VanBuskirk AM

    更新日期:2005-02-15 00:00:00

  • Vascular remodeling of the vitelline artery initiates extravascular emergence of hematopoietic clusters.

    abstract::The vitelline artery is a temporary structure that undergoes extensive remodeling during midgestation to eventually become the superior mesenteric artery (also called the cranial mesenteric artery, in the mouse). Here we show that, during this remodeling process, large clusters of hematopoietic progenitors emerge via ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2010-04-279497

    authors: Zovein AC,Turlo KA,Ponec RM,Lynch MR,Chen KC,Hofmann JJ,Cox TC,Gasson JC,Iruela-Arispe ML

    更新日期:2010-11-04 00:00:00

  • Use of unrelated marrow grafts compensates for reduced graft-versus-leukemia reactivity after T-cell-depleted allogeneic marrow transplantation for chronic myelogenous leukemia.

    abstract::The effect of donor/recipient histocompatibility on relapse in patients receiving T-cell-depleted (TCD) grafts for chronic myelogenous leukemia (CML) was evaluated. Specifically, we sought to determine whether TCD results in an attenuation of the graft-versus-leukemia (GVL) effect on recipients of unrelated marrow gra...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Hessner MJ,Endean DJ,Casper JT,Horowitz MM,Keever-Taylor CA,Roth M,Flomenberg N,Drobyski WR

    更新日期:1995-11-15 00:00:00

  • Correction of the platelet adhesion defect in delta-storage pool deficiency at elevated hematocrit--possible role of adenosine diphosphate.

    abstract::Previous studies on patients with storage pool deficiency (SPD) who are specifically deficient in platelet dense granules (delta-SPD) have suggested a role for dense granule substances, in all likelihood adenosine diphosphate (ADP), in mediating thrombus formation on subendothelium at high shear rates. The role of den...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Weiss HJ,Lages B,Hoffmann T,Turitto VT

    更新日期:1996-05-15 00:00:00

  • Solution structure of the major factor VIII binding region on von Willebrand factor.

    abstract::Although much of the function of von Willebrand factor (VWF) has been revealed, detailed insight into the molecular structure that enables VWF to orchestrate hemostatic processes, in particular factor VIII (FVIII) binding and stabilization in plasma, is lacking. Here, we present the high-resolution solution structure ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2013-07-517086

    authors: Shiltagh N,Kirkpatrick J,Cabrita LD,McKinnon TA,Thalassinos K,Tuddenham EG,Hansen DF

    更新日期:2014-06-26 00:00:00

  • HIV gene therapy research advances.

    abstract::In this issue of Blood, Tebas et al report antiviral effects in a clinical trial of multiple infusions of lentiviral vector–modified autologous CD4T lymphocytes in 17 HIV-infected patients aviremic on antiretroviral therapy (ART). ...

    journal_title:Blood

    pub_type: 评论,杂志文章

    doi:10.1182/blood-2013-01-475921

    authors: Jacobson JM

    更新日期:2013-02-28 00:00:00

  • Activity of transgene-produced B-domain-deleted factor VIII in human plasma following AAV5 gene therapy.

    abstract::Adeno-associated virus (AAV)-based gene therapies can restore endogenous factor VIII (FVIII) expression in hemophilia A (HA). AAV vectors typically use a B-domain-deleted FVIII transgene, such as human FVIII-SQ in valoctocogene roxaparvovec (AAV5-FVIII-SQ). Surprisingly, the activity of transgene-produced FVIII-SQ was...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.2020005683

    authors: Rosen S,Tiefenbacher S,Robinson M,Huang M,Srimani J,Mackenzie D,Christianson T,Pasi KJ,Rangarajan S,Symington E,Giermasz A,Pierce GF,Kim B,Zoog SJ,Vettermann C

    更新日期:2020-11-26 00:00:00

  • Effect of interleukin-1 beta converting enzyme inhibitor on acute myelogenous leukemia progenitor proliferation.

    abstract::Interleukin-1 beta (IL-1 beta) converting enzyme (ICE) is a cysteine protease that specifically cleaves precursor IL-1 beta to its biologically active form. Recent studies have also implicated ICE in the induction of apoptosis in vertebrate cells. Because IL-1 plays a major role in acute myelogenous leukemia (AML) bla...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Estrov Z,Black RA,Sleath PR,Harris D,Van Q,LaPushin R,Estey EH,Talpaz M

    更新日期:1995-12-15 00:00:00

  • ALK-negative anaplastic large cell lymphoma is a genetically heterogeneous disease with widely disparate clinical outcomes.

    abstract::Anaplastic lymphoma kinase (ALK)-negative anaplastic large cell lymphoma (ALCL) is a CD30-positive T-cell non-Hodgkin lymphoma that morphologically resembles ALK-positive ALCL but lacks chromosomal rearrangements of the ALK gene. The genetic and clinical heterogeneity of ALK-negative ALCL has not been delineated. We p...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2014-04-571091

    authors: Parrilla Castellar ER,Jaffe ES,Said JW,Swerdlow SH,Ketterling RP,Knudson RA,Sidhu JS,Hsi ED,Karikehalli S,Jiang L,Vasmatzis G,Gibson SE,Ondrejka S,Nicolae A,Grogg KL,Allmer C,Ristow KM,Wilson WH,Macon WR,Law ME,Ce

    更新日期:2014-08-28 00:00:00

  • Wild-type macrophages reverse disease in heme oxygenase 1-deficient mice.

    abstract::Loss-of-function mutation in the heme oxygenase 1 (Hmox1) gene causes a rare and lethal disease in children, characterized by severe anemia and intravascular hemolysis, with damage to endothelia and kidneys. Previously, we found that macrophages engaged in recycling of red cells were depleted from the tissues of Hmox1...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2014-02-554162

    authors: Kovtunovych G,Ghosh MC,Ollivierre W,Weitzel RP,Eckhaus MA,Tisdale JF,Yachie A,Rouault TA

    更新日期:2014-08-28 00:00:00

  • Identification of CD8alpha+CD11c- lineage phenotype-negative cells in the spleen as committed precursor of CD8alpha+ dendritic cells.

    abstract::CD8alpha+ dendritic cells (DCs) represent a functionally distinct DC subset in vivo, which plays a critical role in initiating various cellular immune responses. However, the committed precursor of CD8alpha+ DCs remains to be identified. We reported here that murine splenic CD8alpha+CD11c- lineage phenotype (Lin)- cel...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v100.2.569

    authors: Wang Y,Zhang Y,Yoneyama H,Onai N,Sato T,Matsushima K

    更新日期:2002-07-15 00:00:00

  • Defects in neutrophil granule mobilization and bactericidal activity in familial hemophagocytic lymphohistiocytosis type 5 (FHL-5) syndrome caused by STXBP2/Munc18-2 mutations.

    abstract::Familial hemophagocytic lymphohistiocytosis (FHL) is caused by genetic defects in cytotoxic granule components or their fusion machinery, leading to impaired natural killer cell and/or T lymphocyte degranulation and/or cytotoxicity. This may accumulate into a life-threatening condition known as macrophage activation s...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2013-03-494039

    authors: Zhao XW,Gazendam RP,Drewniak A,van Houdt M,Tool AT,van Hamme JL,Kustiawan I,Meijer AB,Janssen H,Russell DG,van de Corput L,Tesselaar K,Boelens JJ,Kuhnle I,Van Der Werff Ten Bosch J,Kuijpers TW,van den Berg TK

    更新日期:2013-07-04 00:00:00