Abstract:
:Candida krusei is osmotolerant yeast used for the production of glycerol. Addition of osmolyte such as NaCl into culture medium can increase the production of glycerol from glucose, but osmolytes may burden the glycerol separation. A coencapsulation method was suggested to create local extracellular hyperosmotic stress for glycerol accumulation. Firstly, the influence of osmotic stress induced by the addition of PEG4000 on growth and metabolism of free cell was studied in detail. Glycerol accumulation could be improved by employing PEG4000 as osmoregulator. Secondly, cells and PEG4000 were coentrapped in NaCS/PDMDAAC capsules to create local hyperosmotic stress. The effects of local hyperosmotic microenvironment on the cell growth and metabolism were studied. The coentrapment method increased the glycerol concentration by 25%, and the glycerol concentration attained 50 gL⁻¹ with productivity of 18.8 gL⁻¹Day⁻¹ in shake flask. More importantly, the glycerol could be directly separated from the encapsulated cells. The entrapped cells containing PEG4000 were also cultivated for 15 days in an airlift reactor. The yield and productivity were ca. 35% and 21 gL⁻¹Day⁻¹, respectively.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Chen G,Yao Sdoi
10.1155/2013/467263subject
Has Abstractpub_date
2013-01-01 00:00:00pages
467263eissn
2314-6133issn
2314-6141journal_volume
2013pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVES:Parents' birth weight acts as a predictor for the descendant birth weight, with the correlation more strongly transmitted through maternal line. The present research aims to study the correlation between the child's low or increased birth weight, the mother's birth weight, and maternal conditi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/615034
更新日期:2015-01-01 00:00:00
abstract::In the present work we developed a MPN quantitative real-time PCR (MPN-qPCR) method for a fast and reliable detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed vegetables. In order to validate the proposed technique, the results were compared with conventional MPN...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/608296
更新日期:2014-01-01 00:00:00
abstract::Over the years, studies have demonstrated morphological changes in the brain of fibromyalgia (FMS). We aimed to conduct a coordinate-based meta-analytic research through systemic review on voxel-based morphometry (VBM) imaging results to identify consistent gray matter (GM) difference between FMS patients and healthy ...
journal_title:BioMed research international
pub_type: 杂志文章,meta分析,评审
doi:10.1155/2016/7296125
更新日期:2016-01-01 00:00:00
abstract::A kind of chemically cross-linked pH-sensitive hydrogels based on methoxyl poly(ethylene glycol)-poly(caprolactone)-acryloyl chloride (MPEG-PCL-AC, PECA), poly(ethylene glycol) methyl ether methacrylate (MPEGMA, MEG), N,N-methylenebisacrylamide (BIS), and itaconic acid (IA) were prepared without using any organic solv...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/239838
更新日期:2013-01-01 00:00:00
abstract::The small nucleolar RNA host gene 12 (SNHG12) has been reported to play an important role in the tumorigenesis and progression of PCa, but the functional underlying mechanism has not been studied clearly. We detected the expression level of SNHG12 in PCa tissues and matched adjacent normal tissues that were collected ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8812923
更新日期:2020-12-22 00:00:00
abstract::Mucosal melanoma is a rare disease, which differs from its cutaneous counterpart genetically and for its clinical behaviour. Moreover this is a heterogeneous disease based on the tissue of origin. As CT7 and CT10 are highly expressed in cutaneous melanoma and are immunogenic in this disease, we analysed their expressi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/432479
更新日期:2015-01-01 00:00:00
abstract::This study shows that membrane-associated cytoskeletal protein structures and the erythrocyte morphology undergo profound changes during hypoxia. Hypoxia also intensified oxidative processes in the lipid phase of the bilayer of red blood cell membranes. Sodium nitroprusside impaired the morphology of red blood cells a...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6758017
更新日期:2019-12-07 00:00:00
abstract::SARS-CoV-2 pandemic and COVID-19 diffusion have recently become an international public health emergency. Cancer patients, as a frail population, are particularly exposed to the risk related to infections. The clinical decision-making process and the organizational workflow of radiotherapy department should be revised...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/4892382
更新日期:2020-05-26 00:00:00
abstract::Mild cognitive impairment (MCI) refers to a transitional state between normal aging and dementia and is a syndrome with cognitive decline greater than expected for an individual's age and educational level. As a subtype of MCI, amnestic mild cognitive impairment (aMCI) most often leads to Alzheimer's disease. This stu...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/920902
更新日期:2014-01-01 00:00:00
abstract::We aimed to predict the ten-year cardiovascular disease (CVD) risk among low-income urban dwellers of metropolitan Malaysia. Participants were selected from a cross-sectional survey conducted in Kuala Lumpur. To assess the 10-year CVD risk, we employed the Framingham risk scoring (FRS) models. Significant determinants...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/516984
更新日期:2015-01-01 00:00:00
abstract::[This retracts the article DOI: 10.1155/2014/479180.]. ...
journal_title:BioMed research international
pub_type: 撤回出版物
doi:10.1155/2016/4938464
更新日期:2016-01-01 00:00:00
abstract::Subtle perturbations in intraglomerular VEGF/VEGFR-2 signaling or in the influencing microenvironment can profoundly affect renal function, resulting in the apparently paradoxical observation that VEGF blockade attenuates proteinuria development in experimental diabetes despite exerting the opposite effect under other...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/201475
更新日期:2013-01-01 00:00:00
abstract::The purpose of the study is to evaluate the relationship between NIH-CPSI and IIEF-5 in Chinese men with CP/CPPS. A large cross-sectional and multicenter survey was conducted from July 2012 to January 2014. Men were recruited from urology clinics which were located at the five cities in China. All men participated in ...
journal_title:BioMed research international
pub_type: 杂志文章,多中心研究
doi:10.1155/2015/560239
更新日期:2015-01-01 00:00:00
abstract::Paclitaxel, a class of taxane with microtubule stabilising ability, has remained with platinum based therapy, the standard care for primary ovarian cancer management. A deeper understanding of the immunological basis and other potential mechanisms of action together with new dosing schedules and/or routes of administr...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/413076
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The aims of this study were to evaluate the complications that occur after trauma and the characteristics of individuals who develop complications, to identify potential risk factors that increase their incidence, and finally to investigate the relationship between complications and mortality. METHODS:We di...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/473419
更新日期:2014-01-01 00:00:00
abstract::Background. The chronic recommendation of pediatric drugs could exhibit erosive and cariogenic problems. Objective. To evaluate the effects of different pediatric drugs on the color stability of various restorative materials. Methods. Five specimens (1 mm × 3 mm) were prepared and immersed in ten different pediatric d...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9684193
更新日期:2017-01-01 00:00:00
abstract::The purpose of the present study was to examine the convergent and divergent validity of the Biber Cognitive Estimation Test (BCET) in individuals with ABI undergoing postacute rehabilitation and to assess the measure's ability to account for unique variance in community integration following rehabilitation. Participa...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2874819
更新日期:2017-01-01 00:00:00
abstract::Introduction. NOTCH pathway and TP53 protein are involved in the development of fibrosis and autoimmune disorders, respectively. The aim of this study was to evaluate the role of single nucleotide polymorphisms (SNPs) of NOTCH3 and TP53 genes and serum anti-TP53 antibodies with the susceptibility, clinical subset of s...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8465971
更新日期:2020-02-25 00:00:00
abstract:Purpose:To evaluate the relationships between objectively measured physical activity and physical fitness among preschool children. Methods:A total of 346 participants (201 boys and 145 girls) aged 3.5-5.5 years (M = 4.5 yr, SD = 0.47) from Shanghai, China, completed physical fitness assessments, including triceps ski...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9314026
更新日期:2017-01-01 00:00:00
abstract::Somatostatin (SST) receptors (SSTRs) belong to the typical 7-transmembrane domain family of G-protein-coupled receptors. Five distinct subtypes (termed SSTR1-5) have been identified, with SSTR2 showing the highest affinity for natural SST and synthetic SST analogs. Most neuroendocrine tumors (NETs) have high expressio...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/917968
更新日期:2015-01-01 00:00:00
abstract::The principle mechanism of protection of stem cells is through the expression of ATP-binding cassette (ABC) transporters. These transporters serve as the guardians of the stem cell population in the body. Unfortunately these very same ABC efflux pumps afford protection to cancer stem cells in tumors, shielding them fr...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/635745
更新日期:2015-01-01 00:00:00
abstract::Interactions between proteins and drugs, which can lead to formation of stable drug-protein complexes, have important implications on several processes related to human health. These interactions can affect, for instance, free concentration, biological activity, and metabolism of the drugs in the blood stream. Here, w...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/872701
更新日期:2014-01-01 00:00:00
abstract:Objectives:Magnetic resonance imaging (MRI) and in particular diffusion-weighted imaging (DWI) have been broadly proven to be the reference imaging method to discriminate between cholesteatoma and noncholesteatomatous middle ear lesions, especially when high tissue specificity is required. The aim of this study is to d...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/7945482
更新日期:2018-04-24 00:00:00
abstract::One of the biggest obstetric challenges is the diagnosis and management of a short cervix as cervical length has an inverse relationship with risk of preterm birth. A cervical cerclage is a surgical procedure to reduce the risk of preterm birth and can be placed in an elective or emergency setting. This is a retrospec...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1155/2018/2065232
更新日期:2018-09-30 00:00:00
abstract::Objective. This study was aimed at analyzing the expressions of long noncoding RNAs (lncRNAs) in Botulinum Toxin Type A (BoNTA) treated human dermal fibroblasts (HDFs) in vitro. Methods. We used RNA sequencing to characterize the lncRNAs and mRNAs transcriptome in the control and BoNTA treated group, in conjunction wi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2957941
更新日期:2017-01-01 00:00:00
abstract::WHO reported that adherence among patients with chronic diseases averages only 50% in developed countries. This is recognized as a significant public health issue, since medication nonadherence leads to poor health outcomes and increased healthcare costs. Improving medication adherence is, therefore, crucial and revea...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/217047
更新日期:2015-01-01 00:00:00
abstract::Severe acute pancreatitis (SAP) results in high mortality. This is partly because of early multiple organ dysfunction syndromes that are usually caused by systemic inflammatory response syndrome (SIRS). Many studies have reported the beneficial effects of emodin against SAP with SIRS. However, the exact mechanism unde...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/1736024
更新日期:2016-01-01 00:00:00
abstract::Tripartite Motif Containing 11 (TRIM11), an E3 ubiquitin ligase, is identified as a carcinogen causing certain human cancers. However, the specific role of TRIM11 is still uncovered in human osteosarcoma (OS) cells. To explore the role of TRIM11 in OS cells, TRIM11 was induced by silencing and overexpression in OS cel...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/9612125
更新日期:2019-12-25 00:00:00
abstract::Autophagy, a lysosomal catabolic pathway for long-lived proteins and damaged organelles, is crucial for cell homeostasis, and survival under stressful conditions. During starvation, autophagy is induced in numerous organisms ranging from yeast to mammals, and promotes survival by supplying nutrients and energy. In the...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/701064
更新日期:2014-01-01 00:00:00
abstract:Background:DNA methylation is essential for regulating gene expression, and the changes of DNA methylation status are commonly discovered in disease. Therefore, identification of differentially methylation patterns, especially differentially methylated regions (DMRs), in two different groups is important for understand...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1070645
更新日期:2018-11-18 00:00:00