Morphology and ultrastructure of the freshwater rotifer Brachionus bidentatus (Monogononta: Brachionidae) using scanning and transmission electron microscopy.

Abstract:

:The study of sexual reproductive behavior supported by ultrastructural evidence is important in rotifers to describe differences among potential cryptic species. In this research, the morphology of the rotifer Brachionus bidentatus is described at the ultrastructural level, using electronic microscopy, together with a brief description and discussion of its sexual reproductive behavior. The characteristics of the (a) male, (b) the female, (c) the sexual egg or cyst, (d) the partenogenic egg, (e) the no-fecundated sexual egg (male egg), and (f) the trophi, were described. Another part of this research is dedicated to the ultrastructure of the sex cells of the male rotifer B. bidentatus. Samples were obtained from La Punta pond in Cosio, Aguascalientes, Mexico (22 degrees 08' N - 102 degrees 24' W), and a culture was maintained in the laboratory. Fifty organisms, from different stages of the rotifer Brachionus bidentatus, were fixed in Formol at 4% and then prepared; besides, for the trophi, 25 female rotifer Brachionus bidentatus were prepared for observation in a JEOL 5900 LV scanning electronic microscope. In addition, for the observation of male sex cells, 500 males of Brachionus bidentatus were isolated, fixed and observed in a JEOL 1010 transmission microscope. Females of B. bidentatus in laboratory cultures had a lifespan of five days (mean+one SD = 4.69 +/- 0.48; N=13), and produced 4.5 +/- 3.67 (N=6) parthenogenetic eggs during such lifespan. In the case of non-fertilized sexual eggs, they produced up to 18 eggs (mean+one SD = 13 +/- 4.93; N=7). Sexual females produced a single cyst on average (mean +/- one SD = I +/- 0; N=20). For the sexual cycle, the time of copulation between male and female ranged from 10 to 40 seconds (mean +/- one SD = 17.33 +/- 10.55, N=7). The spermatozoa are composed of a celular body and a flagellum, the size of the body is of 300 nm while the flagellum measures 1 700nm. The rods have a double membrane. Their mean length is almost 2.45 microm +/- 0.74, N=6; and their mean wide is 0.773 microm +/- 0.241, N=11. The evidence on the specific ultrastructural characteristics of the rotifer B. bidentatus is notorious, even more in the male and in the cyst cell. Regarding the ultrastructure of the spermatozoa and the rods, compared to other species they only differ in size, despite their structural resemblance. Our study of the ultraestructure of this species adds useful information that along with molecular data will help clarify the taxonomy of brachionid rotifers.

journal_name

Rev Biol Trop

authors

Guerrero-Jiménez G,Zavala-Padilla G,Silva-Briano M,Rico-Martínez R

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

1737-45

issue

4

eissn

0034-7744

issn

2215-2075

journal_volume

61

pub_type

杂志文章
  • [Impact of two artificial reefs on diurnal artisanal fishing at gulf of Morrosquillo, Colombian Caribbean].

    abstract::Fishing activity in two artificial reefs (ARs) was monitored between August 2001 and August 2002 in the gulf of Morrosquillo, to investigate their possible impact on the fishery dynamics. We determined catch per unit effort (CPUE) and catch composition, and found 19 species in Punta de Piedra and 36 in Tolú (16 specie...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Delgadillo-Garzón O,García CB

    更新日期:2009-12-01 00:00:00

  • Paternal behavior and testosterone plasma levels in the Volcano Mouse Neotomodon alstoni (Rodentia: Muridae).

    abstract::Paternal behavior and testosterone plasma levels in the Volcano Mouse Neotomodon alstoni (Rodentia: Muridae). Although initially it was thought that testosterone inhibited the display of paternal behavior in males of rodents, it has been shown that in some species high testosterone levels are needed for exhibition of ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:10.15517/rbt.v57i1-2.11360

    authors: Luis J,Ramírez L,Carmona A,Ortiz G,Delgado J,Cárdenas R

    更新日期:2009-03-01 00:00:00

  • [The seeds of Campsiandra angustifolia (Fabaceae: Caesalpiniodeae) as a reflex of selective pressures on dispersal and establishment].

    abstract::We indirectly evaluated the selective pressures on dispersal and establishment of Campsiandra angustifolia, a common water-dispersed tree from the Peruvian Amazon, analyzing the variation in the relationship between the volume occupied by dispersal and establishment structures in a total of 535 seeds from 13 trees loc...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Farji-Brener AG,Durán S,Valerio A,Herbas E,Castañeda M,Ochoa J,Romo M

    更新日期:2005-03-01 00:00:00

  • [Floristic composition and structure of a premontane moist forest in Central Valley of Costa Rica].

    abstract::The floristic composition and structure of a premontane moist forest remnant were studied in the El Rodeo Protected Zone, Central Valley of Costa Rica. Three one-hectare plots were established in the non-disturbed forest, and all trees with a diameter at breast height (dbh) of 10 cm or greater were marked, measured an...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Cascante A,Estrada A

    更新日期:2001-03-01 00:00:00

  • [Current and future ecological niche of Leishmaniasis (Kinetoplastida: Trypanosomatidae) in the Neotropical region].

    abstract::The leishmaniasis is a complex disease system, caused by the protozoan parasite Leishmania and transmitted to humans by the vector Lutzomyia spp. Since it is listed as a neglected disease according to the World Health Organization, the aim of this study was to determine the current and future niche of cutaneous and vi...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Moo-Llanes DA

    更新日期:2016-09-01 00:00:00

  • Development and distribution of cysts of an avirulent strain of Toxoplasma and the humoral immune response in mice.

    abstract::An avirulent T. gondii strain isolated from a owl (Glaucidium brasilianum) produces randomly distributed cysts in the brain of mice which can survive to inocula as high as 1000 oocysts. Cysts appeared for the first time after 15 days of infection. Regarding to humoral immunity development due to our TCR-2 strain, dete...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Holst I,Chinchilla M

    更新日期:1990-11-01 00:00:00

  • [Physical-chemical features and determination of pesticides in the lagoon water in Gandoca, Limón, Costa Rica. ].

    abstract::Nutrients and chlorophylls concentrations, as well as salinity, temperature and Secchi disk depth were determined from November 1999 to April 2000, at three stations and two depths, at Gandoca lagoon, Gandoca-Manzanillo National Wildlife Refuge, Limón, Costa Rica. Salinity profiles indicated that the lagoon was a salt...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Coll M,Cortés J,Sauma D

    更新日期:2004-12-01 00:00:00

  • Transplantation of benthic species to mitigate impacts of coastal development in Jamaica.

    abstract::Maintaining regional competitiveness and economic viability for Port Bustamante - Kingston Harbour, Jamaica, required improved accessibility to "Post Panamax" (too large to pass through the Panama Canal) container vessels. Removal of the northern portion of the shallow coral reef at Rackham's Cay, which was partially ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Gayle PM,Wilson-Kelly P,Green S

    更新日期:2005-05-01 00:00:00

  • Biodiversity of freshwater fish of a protected river in India: comparison with unprotected habitat.

    abstract::In India, freshwater environments are experiencing serious threats to biodiversity, and there is an urgent priority for the search of alternative techniques to promote fish biodiversity conservation and management. With this aim, the present study was undertaken to assess the fish biodiversity within and outside a riv...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:10.15517/rbt.v61i1.10942

    authors: Sarkar UK,Pathak AK,Tyagi LK,Srivastava SM,Singh SP,Dubey VK

    更新日期:2013-03-01 00:00:00

  • [Cytogenetic characterization of the neotropical fish Brycon henni (Pisces: Characidae)].

    abstract::Brycon henni, is a native fish from Western Colombia is important as food and in sport fishing, and could be cultured in waters between 18 and 28 degrees C. A previous cytogenetic study in branchial cells indicated different chromosomal complements. Total blood samples were seeded in 4 ml Ham-F12 culture medium, suppl...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: López DD,Vásquez Palacio G,Ruiz Cortés T,Olivera Angel M

    更新日期:2008-12-01 00:00:00

  • Underwater topography determines critical breeding habitat for humpback whales near Osa Peninsula, Costa Rica: implications for marine protected areas.

    abstract::Migrating humpback whales from northern and southern feeding grounds come to the tropical waters near Osa Peninsula, Pacific of Costa Rica, to reproduce and raise their calves. Planning effective marine protected areas that encompass humpback critical habitats require data about which oceanographic features influence ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:10.15517/rbt.v56i2.5610

    authors: Oviedo L,Solís M

    更新日期:2008-06-01 00:00:00

  • [The benthic fauna of Sabancuy Estuary, Campeche, Mexico].

    abstract::The fish and invertebrates community structure in the Sabancuy estuary was analyzed in two seasons and 14 sampling stations (13 along the estuary and one in the marine adjacent coast). No significant differences were found between seasons. The environmental frame defines two zones within the estuary, the first extends...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: González Solís A,Torruco Gómez D

    更新日期:2001-03-01 00:00:00

  • [Vegetation diversity, composition and structure in a cattle agro-landscape of Matiguás, Nicaragua].

    abstract::The diversity, composition and structure of vegetation in a cattle landscape in Matiguás, Nicaragua was characterized, and the floristic and structural differences of six types of habitats (secondary forests, riparian forests, charrales, live fences and pastures with high and low tree cover) were compared. A total of ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Merlos DS,Harvey CA,Grijalva A,Medina A,Vílchez S,Hernández B

    更新日期:2005-09-01 00:00:00

  • [In vitro effect of an alkaloid isolated from Amphimedon viridis (Porifea) on promastigots of Leishmania mexicana].

    abstract::The dose dependent antiproliferative effect of an alkaloidal substance extracted from the sponge Amphimedon viridis was tested on Leishmania mexicana promastigotes. Sponges were collected in Isla Larga, Venezuela (10 degrees 20' 20" - 10 degrees 24" N, 64 degrees 19' - 64 degrees 22' W), cut and dipped in methanol for...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Marchán E,Arrieche D,Henríquez W,Crescente O

    更新日期:2000-12-01 00:00:00

  • [Echinoderms (Echinodermata) from the Gulf of California, Mexico].

    abstract::A systematic list of the echinoderms of the Gulf of California, based on museum specimens of the Colección Nacional de Equinodermos, Instituto de Ciencias del Mar y Limnologia, Universidad Nacional Autónoma de México and the National Museum of Natural History, Smithsonian Institution, Washington, D.C. is presented. A ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Solís-Marín FA,Laguarda-Figueras A,Durán-González A,Ahearn CG,Torres Vega J

    更新日期:2005-12-01 00:00:00

  • [Reproductive biology of Anisotremus interruptus (Perciformes: Haemulidae) in the Mexican Central Pacific].

    abstract::Anisotremus interruptus is a widely distributed and commercially important species in the Eastern Pacific. A multi-year research on the reproduction of this species was carried out in coastal waters of Jalisco, Mexico. For this purpose, monthly samples were gathered with gillnets of different mesh sizes from 1998-2008...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Ruiz-Ramírez S,Lucano-Ramírez G,González-Sansón G,Rojo-Vázquez JA,Arellano-Martínez M

    更新日期:2012-06-01 00:00:00

  • [Identification of individual jaguars (Panthera onca) and pumas (Puma concolor) based on footprint morphometry (Carnivora: Felidae)].

    abstract::Estimating feline abundance becomes particularly difficult, sometimes impossible, due to their elusive behavior and extensive space requirements. Available techniques are expensive and/or poorly efficient, therefore alternative methods are needed. The objective of this study was to assess the possibility of identifyin...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Isasi-Catalá E,Barreto GR

    更新日期:2008-12-01 00:00:00

  • Influence of joint application of heavy metals on level of each metal accumulated in the periwinkle Tympanotonus fuscatus (Gastropoda: Potamididae).

    abstract::Most laboratory assessments of toxicity and bioaccumulation of heavy metals have been concentrated on the accumulation of these metal ions when exposed singly to the test organisms. However, under the natural environmental settings, the metals are never present in isolation and may interact with each other, therefore ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:10.15517/rbt.v54i3.12791

    authors: Otitoloju AA,Don-Pedro KN

    更新日期:2006-09-01 00:00:00

  • [Redescription of Parodon caliensis and Saccodon dariensis (Characiformes: Parodontidae)].

    abstract::Redescription of Parodon caliensis and Saccodon dariensis (Characiformes: Parodontidae). Parodontidae family is a group of Characiformes fishes distributed throughout South America and parts of Panama, except in the basins of the southern Bahia state in Brasil on the Atlantic coast, Patagonia and the Amazon river chan...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Londoño-Burbano A,Román-Valencia C

    更新日期:2010-09-01 00:00:00

  • Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia).

    abstract::Coral reefs are impacted by a range of environmental variables that affect their growth and survival, the main factors being the high irradiance and temperature fluctuations. Specimens of Pocillopora capitata Verrill 1864 were exposed to photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) for 32 ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:10.15517/rbt.v58i1.5197

    authors: Liñán-Cabello MA,Flores-Ramírez LA,Cobo-Díaz JF,Zenteno-Savin T,Olguín-Monroy NO,Olivos-Ortiz A,Tintos-Gómez A

    更新日期:2010-03-01 00:00:00

  • [Ecological ensembles and assemblages - a reply to Monge-Nájera].

    abstract::Critical evaluation of terminology use in ecology is important to avoid using jargon that helps little to facilitate communication. Here we reply to Monge-Najera’s comment on the use of the terms ensemble and assemblage, we argue for the use of the words ensamble and ensamblaje in Spanish. In addition, both terms have...

    journal_title:Revista de biologia tropical

    pub_type: 评论,信件

    doi:

    authors: Ramírez A,Gutiérrez-Fonseca PE

    更新日期:2016-06-01 00:00:00

  • [Structure of the Collembolan community (Hexapoda: Collembola) in bark mosses along an altitudinal gradient of a subhumid forest in Mexico].

    abstract::The structure of the community of moss springtails on oak and fir bark in the forests at the NW slope of Iztaccihuatl Volcano, State of Mexico, is analyzed. The study included four samplings (November 2003, March-June-August 2004), carried out in three altitudes I: 2750 m, II: 2 930 and 3 250 m a.s.l. Calculated index...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Cutz-Pool LQ,Palacios-Vargas JG,Castaño-Meneses G

    更新日期:2008-06-01 00:00:00

  • [Determination of stress in fish community obtained from shrimp trawl fishing in Northern Gulf of California].

    abstract::Bottom trawling has been considered a fishing activity that affects and modifies habitats, because of its impacts in species composition and abundance, and the alteration in the structure and function of the eco-system, that generates biodiversity loss. The Northern part of the Gulf of California has been considered a...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Herrera-Valdivia E,López-Martínez J,Vargasmachuca SC

    更新日期:2015-09-01 00:00:00

  • On Limnocytherina axalapasco, a new freshwater ostracod (Podocopida: Limnocytheridae) from Mexican crater lakes.

    abstract::Limnocytherina is a genus conformed by 12 species; its distribution in the American continent is known to be exclusively on the North (neartics), but little is reported about its distribution from Mexico (transition zone) and Central America (Neotropics). Different sampling campaigns were undertaken in three crater la...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Cohuo-Durán S,Pérez L,Karanovic I

    更新日期:2014-03-01 00:00:00

  • [Population structure and environmental relationships of the tropical tree Nectandra rudis (Lauraceae), a rare species in western Mexico].

    abstract::Population structure and environmental relationships of the tropical tree Nectandra rudis (Lauraceae), a rare species in western Mexico. The tree N. rudis is a rare species from western Mexico of which community and population features are unknown. We studied a population in an altitudinal gradient, from 550-1,850 m a...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Guzmán RC,Moya EG,García JA,López NM

    更新日期:2008-03-01 00:00:00

  • Temporal and spatial resource use by female three-toed sloths and their young in an agricultural landscape in Costa Rica.

    abstract::The information on ecological behavior of wild sloths is very scarce. In this study we determined the home ranges and resources used by three adult female three-toed sloths (Bradypus variegatus) and their four young in an agricultural matrix of cacao (Theobroma cacao), pasture, riparian forests and living fencerows in...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Ramirez O,Vaughan C,Herrera G,Guries R

    更新日期:2011-12-01 00:00:00

  • [Serpulids (Polychaeta: Serpulidae) from the Northwestern Caribbean: Hydroides and Serpula].

    abstract::From the study of more than 450 specimens of Hydroides and Serpula, 12 species from the Grand Caribbean Region were identified and characterized. Eight species were collected along the shores of the Yucatan Peninsula and five were found in other localities in the Gulf of Mexico, seven others are from Cuba and comments...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Bastida-Zavala JR,Salazar-Vallejo SI

    更新日期:2000-12-01 00:00:00

  • [Diet of the capybara Hydrochaeris hydrochaeris (Rodentia: Hydrocharidae) in Caño Limón, Arauca, Colombia].

    abstract::We studied the composition and seasonal variation of the diet of the capybara (Hydrochaeris hydrochaeris) in the flooded savannas of Caño Limón, Colombia. This was achieved by direct observation of the consumption patterns of these animals. The capybaras only consumed plants, and their diet included 89 species of 22 f...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Forero-Montaña J,Betancur J,Cavelier J

    更新日期:2003-06-01 00:00:00

  • Black corals (Cnidaria: Anthozoa: Antipatharia): first records and a new species from the Brazilian coast.

    abstract::A new species, Cirripathes secchini (Cnidaria: Antipatharia) from the Abrolhos area (Bahia, Brazil) is described and constitutes the first record to this genus from Brazil. Two other species (Antipathes hirta, first record from the Southwestern Atlantic; and Antipathes fernandezi, first record from the Atlantic Ocean)...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Echeverría CA

    更新日期:2002-09-01 00:00:00

  • Temporal and spatial distribution patterns of echinoderm larvae in La Parguera, Puerto Rico.

    abstract::This study describes temporal and spatial abundance patterns of echinoderm larvae in La Parguera, Puerto Rico. For the temporal study, larvae were sampled by a series of monthly tows taken with a 64 microm mesh net between the new and full moon from April 2005 to July 2006, September 2006 and August 2007. In order to ...

    journal_title:Revista de biologia tropical

    pub_type: 杂志文章

    doi:

    authors: Williams SM,Jorge GS

    更新日期:2010-10-01 00:00:00