Abstract:
:Light-dark cycles are considered important cues to entrain biological clocks. A feedback loop of clock gene transcription and translation is the molecular basis underlying the mechanism of both central and peripheral clocks. Xenopus laevis embryonic melanophores respond to light with melanin granule dispersion, response possibly mediated by the photopigment melanopsin. To test whether light modulates clock gene expression in Xenopus melanophores, we used qPCR to evaluate the relative mRNA levels of Per1, Per2, Clock and Bmal1 in cultured melanophores exposed to light-dark (LD) cycle or constant darkness (DD). LD cycles elicited temporal changes in the expression of Per1, Per2 and Bmal1. A 10-min pulse of blue light was able to increases the expression of Per1 and Per2. Red light had no effect on the expression of these clock genes. These data suggest the participation of a blue-wavelength sensitive pigment in the light-dark cycle-mediated oscillation of the endogenous clock. Our results add an important contribution to the emerging field of peripheral clocks, which in nonmammalian vertebrates have been mostly studied in Drosophila and Danio rerio. Within this context, we show that X. laevis melanophores, which have already led to melanopsin discovery, represent an ideal model to understanding circadian rhythms.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Magalhães Moraes MN,de Oliveira Poletini M,Ribeiro Ramos BC,de Lima LH,de Lauro Castrucci AMdoi
10.1111/php.12230subject
Has Abstractpub_date
2014-05-01 00:00:00pages
696-701issue
3eissn
0031-8655issn
1751-1097journal_volume
90pub_type
杂志文章abstract::A widely published expression for dipole strengths of optical transitions is found to require correction. The proposed adjustment, which involves the refractive index of the solvent in which the strengths are measured, may lead to significant changes in predictions that have been based on the equation. We discuss a cl...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)077<0492:DAOSOC>2.0.CO;2
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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abstract::UV radiation is a potent DNA damaging agent and a known inducer of skin cancer in experimental animals. There is excellent scientific evidence to indicate that most non-melanoma human skin cancers are induced by repeated exposure to sunlight. UV radiation is unique in that it induces DNA damage that differs from the l...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:1990-12-01 00:00:00
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13089
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 临床试验,杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-05-31-RA-558
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12124
更新日期:2013-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 临床试验,杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1991.tb01992.x
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00