Induction of refractoriness of cyclic AMP responses to prostaglandin E1 and epinephrine by prior exposure of guinea pig macrophages to lipopolysaccharide.

abstract：:The St-20 and ST-40 cDNAs encode rat liver hydroxysteroid sulfotransferases (HS-ST) that are 90% identical in amino acid sequence but exhibit different substrate preferences for dehydroepiandrosterone (DHEA), androsterone (AD), and cortisol (CS). ST-40 is active for all three substrates, whereas ST-20 is mainly active...

journal_title：Archives of biochemistry and biophysics

authors： Tamura H,Morioka Y,Homma H,Matsui M

更新日期：1997-05-15 00:00:00

abstract：:An attempt was made to relate the iron and phosphate content of ferritin to its subunit composition. Ferritins from various tissues were separated according to their subunit composition by anion exchange chromatography and according to their iron content by density-gradient centrifugation. Iron and phosphate contents ...

journal_title：Archives of biochemistry and biophysics

authors： Juan SH,Aust SD

更新日期：1998-09-15 00:00:00

abstract：:Several 2-halo-acyl-CoA derivatives have been synthesized to examine the interaction of these potential inhibitors of mitochondrial beta-oxidation with the purified medium chain acyl-CoA dehydrogenase from pig kidney. Racemic 2-bromo-, 2-chloro-, and 2-fluoro-octanoyl-CoA thioesters show 6.6, 33, and 3.5% of the activ...

journal_title：Archives of biochemistry and biophysics

authors： Cummings JG,Thorpe C

更新日期：1993-04-01 00:00:00

abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

journal_title：Archives of biochemistry and biophysics

authors： Blair JB,Walker RG

更新日期：1984-07-01 00:00:00

abstract：:The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CG...

journal_title：Archives of biochemistry and biophysics

authors： Quill TA,Hedrick JL

更新日期：1996-09-15 00:00:00

abstract：:This investigation presents disturbances of the mitochondrial metabolism by arsenite, a hydrophilic dithiol reagent known as an inhibitor of mitochondrial alpha-keto acid dehydrogenases. Arsenite at concentrations of 0.1-1.0 mM was shown to induce a considerable oxidation of intramitochondrial NADPH, NADH, and glutath...

journal_title：Archives of biochemistry and biophysics

authors： Lenartowicz E

更新日期：1990-12-01 00:00:00

abstract：:A10 smooth muscle cells, derived from embryonic rat thoracic aorta, responded to the atrial natriuretic factor (ANF) with increased levels of cyclic GMP. These cells possess high-affinity (apparent Kd = 50 pM) plasma membrane receptors for ANF. Internalization of ANF at 37 degrees C was indicated by the following: app...

journal_title：Archives of biochemistry and biophysics

authors： Napier MA,Arcuri KE,Vandlen RL

更新日期：1986-08-01 00:00:00

abstract：:Reversible oxidation of Cys residues is a crucial element of redox homeostasis and signaling. According to a popular concept in oxidative stress signaling, the oxidation of targets of signals can only take place following an overwhelming of the cellular antioxidant capacity. This concept, however, ignores the activati...

journal_title：Archives of biochemistry and biophysics

authors： Zaccarin M,Bosello-Travain V,Di Paolo ML,Falda M,Maiorino M,Miotto G,Piccolo S,Roveri A,Ursini F,Venerando R,Toppo S

更新日期：2017-03-01 00:00:00

abstract：:The oxidation of 2-(methylseleno)benzanilide and 2-selenylbenzanilide, metabolites of the antioxidant drug ebselen, was examined in reactions catalyzed by rat, pig, and guinea pig liver microsomes and in perfused rat liver. Microsomes from all three species catalyzed NADPH- and oxygen-dependent oxidation of the seleni...

journal_title：Archives of biochemistry and biophysics

authors： Akerboom TP,Sies H,Ziegler DM

更新日期：1995-01-10 00:00:00

abstract：:Proliferation and migratory behavior of L929 murine fibroblasts were shown to be modified in the presence of a cytosolic extract of Phormidium sp. (Cyanobacteria). The addition of Phormidium extract to the growth medium (Dulbecco's modified Eagle's medium) supplemented with 0.5% newborn calf serum increased cell proli...

journal_title：Archives of biochemistry and biophysics

authors： Hodara ML,Bré MH,Bouzinba H,Pouphile M,Lefort-Tran M

更新日期：1991-04-01 00:00:00

abstract：:Cytosolic extracts from the posterior adductor muscle of the bivalve mollusk Mytilus galloprovincialis contain significant amounts of both cGMP-binding and cGMP-stimulated protein kinase activities. However, photoaffinity labeling with 8-azido-[32P]cGMP revealed only a major cGMP-binding protein with an apparent molec...

journal_title：Archives of biochemistry and biophysics

authors： Rodríguez JL,Barcia R,Ramos-Martínez JI,Villamarín JA

更新日期：1998-11-01 00:00:00

abstract：:Characterization of aldose reductase purified from human placenta confirms that activation, as first analyzed in detail for the bovine enzyme, also occurs in humans. Routinely between 5 and 20% of the aldose reductase activity freshly purified from human placenta exhibits kinetic properties and insensitivity to aldose...

journal_title：Archives of biochemistry and biophysics

authors： Grimshaw CE,Lai CJ

更新日期：1996-03-01 00:00:00

abstract：:Retinol (vitamin A alcohol), which plays an important role in the differentiation of epithelia, can be transferred to chromatin in vitro. Rat liver chromatin can accept retinol in a specific and saturable manner only when the retinol is presented as a complex with cellular retinol-binding protein (CRBP). A partial cha...

journal_title：Archives of biochemistry and biophysics

authors： Liau G,Ong DE,Chytil F

更新日期：1985-03-01 00:00:00

abstract：:HIV-1 trans-activator of transcription (Tat) is an unusual transcriptional activator in being an RNA-binding protein rather than a DNA-binding protein. Recent findings have greatly advanced our understanding of the transcriptional function(s) of this protein. Here we review how Tat interacts with trans-activation resp...

journal_title：Archives of biochemistry and biophysics

authors： Rana TM,Jeang KT

更新日期：1999-05-15 00:00:00

abstract：:The interaction of reducing sugars with proteins leads to the formation of advanced glycation end products (AGE) and reactive oxidative species (ROS). ROS peroxidise free or membrane included unsaturated fatty acids, leading to generate reactive aldehydes as advanced lipid peroxidation end products (ALE). Aldehydes fr...

journal_title：Archives of biochemistry and biophysics

authors： Nooshi-Nedamani S,Habibi-Rezaei M,Farzadfard A,Moosavi-Movahedi AA

更新日期：2019-06-15 00:00:00

abstract：:Catalases are biotechnologically relevant enzymes because of their applications in food technology, bioremediation, and biomedicine. The dismutation of hydrogen peroxide occurs in two steps; in the first one, the enzyme forms an oxidized compound I (Cpd I) and in the second one, the enzyme is reduced to the ferric sta...

journal_title：Archives of biochemistry and biophysics

authors： Zárate-Romero A,Stojanoff V,Cohen AE,Hansberg W,Rudiño-Piñera E

更新日期：2019-05-15 00:00:00

abstract：:D-Proline is converted to 5-amino valeric acid by D-proline reductase. This conversion involves the reductive cleavage of the alpha-carbon-nitrogen bond. We have examined the fate of the carboxyl oxygen atoms during conversion of D-proline to delta-NH2-valeric acid. 18O atoms from the carboxyl group of D-proline are n...

journal_title：Archives of biochemistry and biophysics

authors： Arkowitz RA,Dhe-Paganon S,Abeles RH

更新日期：1994-06-01 00:00:00

abstract：:To study the relationship between neutral aminopeptidase activity and hemoglobin accumulation in malaria parasites, we treated mice infected with Plasmodium berghei NYU-2 with chloroquine intraperitoneally in doses ranging from 0.3 to 3 micromol per 25 g mouse. Preparations of infected erythrocytes (normalized to repr...

journal_title：Archives of biochemistry and biophysics

authors： Fitch CD,Cai GZ,Chen YF,Ryerse JS

更新日期：2003-02-15 00:00:00

abstract：:Affinity columns prepared by immobilizing monoclonal antibodies that specifically recognize the Lea or the Leb blood group antigens can be used for analytical or preparative isolation of oligosaccharides with the corresponding reactivities. The number of immobilized functional antibody combining sites on a column and ...

journal_title：Archives of biochemistry and biophysics

authors： Dakour J,Lundblad A,Zopf D

更新日期：1988-07-01 00:00:00

abstract：:An anionic peroxidase (EC 1.11.1.7), thought to be involved in suberization, was purified 110-fold from wound-healing slices of Solanum tuberosum by a combination of ammonium sulfate fractionation, Sephadex G-100 gel filtration, isoelectric focusing, and phenyl-Sepharose CL-4B chromatography in 24% yield. The purified...

journal_title：Archives of biochemistry and biophysics

authors： Espelie KE,Kolattukudy PE

更新日期：1985-08-01 00:00:00

abstract：:The kinetic analysis of the enzymatic reaction catalyzed by glycogen phosphorylase b from the rabbit skeletal muscle has been carried out in the direction of glycogen synthesis under the conditions of the saturation of the enzyme by glycogen. The applicability of 12 different variants of the Monod-Wyman-Changeux model...

journal_title：Archives of biochemistry and biophysics

authors： Klinov SV,Kurganov BI

更新日期：1994-07-01 00:00:00

abstract：:The keto form of oxaloacetate (OAA), a product of phosphoenolpyruvate carboxylase (PEPC) activity, can undergo various nonenzymatic conversions which make conventional methods of assaying the enzyme difficult, because the products may either act as inhibitors or go undetected. In studies with PEPC isolated from leaves...

journal_title：Archives of biochemistry and biophysics

authors： Walker GH,Ku MS,Edwards GE

更新日期：1986-08-01 00:00:00

abstract：:Adenosylcobalamin, a coenzyme form of vitamin B12, is an organometallic compound that participates in about ten enzymatic reactions. These enzymes catalyze chemically challenging reactions by using a highly reactive primary carbon radical that is derived from homolysis of the coenzyme Co-C bond. Among them, diol dehyd...

journal_title：Archives of biochemistry and biophysics

authors： Toraya T

更新日期：2014-02-15 00:00:00

abstract：:Recombinant human lymphotoxin (rhLT) produced by CHO cells transfected with human LT genomic DNA was purified to homogeneity, but approximately 5% of the molecules were devoid of the last two amino terminal residues. A peptide N-glycosylated at Asn62 (Tr-45) and one partially O-glycosylated at Thr7 (Tr-14) on cleavage...

journal_title：Archives of biochemistry and biophysics

authors： Fukushima K,Watanabe H,Takeo K,Nomura M,Asahi T,Yamashita K

更新日期：1993-07-01 00:00:00

abstract：:Chronic ethanol intoxication leads to the development of a resistance to lipid disordering by ethanol, a phenomenon known as "membrane tolerance". In the absence of the added ethanol, the lipid order, as measured by ESR and fluorescence techniques, does not necessarily change as a result of chronic ethanol ingestion (...

journal_title：Archives of biochemistry and biophysics

authors： Stubbs CD,Williams BW,Pryor CL,Rubin E

更新日期：1988-05-01 00:00:00

abstract：:A solution hybridization assay employing specific synthetic oligodeoxynucleotide probes was developed to study the regulation of intestinal calbindin-D28K mRNA. The technique is rapid and quantitative and eliminates the need for sample transfer, blotting, autoradiography, and densitometry. Following validation of the ...

journal_title：Archives of biochemistry and biophysics

authors： Fullmer CS

更新日期：1990-11-15 00:00:00

abstract：:The dietary flavan-3-ol (-)-epicatechin improves the bioactivity of nitric oxide in arterial vessels in vivo. Moreover, it effectively protects cultured vascular endothelial cells from signs of oxidative stress and elevates intracellular nitric oxide in vitro. We addressed the effects of (-)-epicatechin, its metabolic...

journal_title：Archives of biochemistry and biophysics

authors： Steffen Y,Gruber C,Schewe T,Sies H

更新日期：2008-01-15 00:00:00

abstract：:Three distinct acid phosphatases were recently reported in avian breast muscle [J. H. Baxter and C. H. Suelter (1984) Arch. Biochem. Biophys. 228, 397-406]. Of the increased acid phosphatase activity in dystrophic muscle compared to normal muscle, 84% can be accounted for as a low-molecular-weight, cytosolic form. Thi...

journal_title：Archives of biochemistry and biophysics

authors： Baxter JH,Suelter CH

更新日期：1985-05-15 00:00:00

abstract：:A human, 85-kDa arachidonoyl-specific, cytosolic phospholipase A2 was expressed using the baculovirus-insect cell expression system. Expression resulted in the production of an active protein which consisted of approximately 3% of the total protein in the host Spodoptera frugiperda (Sf9) cells at 67 h after infection....

journal_title：Archives of biochemistry and biophysics

authors： de Carvalho MS,McCormack FX,Leslie CC

更新日期：1993-11-01 00:00:00

abstract：:Mammalian multidrug and toxic compound extrusion 1 (MATE1) are polyspecific H+-coupled exporters of organic cations (OCs) and responsible for excretion of metabolic waste products and xenobiotics. Here, we report a novel variant of mouse MATE1, mMATE1b, that has a long carboxyl terminal hydrophobic tail homologous to ...

journal_title：Archives of biochemistry and biophysics

authors： Kobara A,Hiasa M,Matsumoto T,Otsuka M,Omote H,Moriyama Y

更新日期：2008-01-15 00:00:00