Abstract:
:Protoplasts were isolated from palisade tissue of tobacco leaves by treatment with pectinase and cellulase under aseptic conditions, and were cultured in a synthetic liquid medium. Calcofluor, a fluorescent brightener, was found to be an excellent stain for plant cell walls and was used to demonstrate regeneration of cell walls in these protoplasts. The cultured protoplasts regenerated cell walls by the 3rd day of culture, giving rise to spherical cells. The majority of the protoplasts regenerating cell walls underwent mitosis and cell division. The cycle of mitosis and cell division was repeated 2-3 times during 2 weeks of culture. Some of the nutritional conditions affecting division in the cultured protoplasts were studied.
journal_name
Plantajournal_title
Plantaauthors
Nagata T,Takebe Idoi
10.1007/BF00385097subject
Has Abstractpub_date
1970-12-01 00:00:00pages
301-8issue
4eissn
0032-0935issn
1432-2048journal_volume
92pub_type
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