Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts.

Abstract:

:Protoplasts were isolated from palisade tissue of tobacco leaves by treatment with pectinase and cellulase under aseptic conditions, and were cultured in a synthetic liquid medium. Calcofluor, a fluorescent brightener, was found to be an excellent stain for plant cell walls and was used to demonstrate regeneration of cell walls in these protoplasts. The cultured protoplasts regenerated cell walls by the 3rd day of culture, giving rise to spherical cells. The majority of the protoplasts regenerating cell walls underwent mitosis and cell division. The cycle of mitosis and cell division was repeated 2-3 times during 2 weeks of culture. Some of the nutritional conditions affecting division in the cultured protoplasts were studied.

journal_name

Planta

journal_title

Planta

authors

Nagata T,Takebe I

doi

10.1007/BF00385097

subject

Has Abstract

pub_date

1970-12-01 00:00:00

pages

301-8

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

92

pub_type

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