Abstract:
BACKGROUND:KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. AIM:To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. MATERIAL AND METHODS:KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing. RESULTS:Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location. CONCLUSIONS:In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation.
journal_name
Rev Med Chiljournal_title
Revista medica de Chileauthors
Roa I,Sánchez T,Majlis A,Schalper Kdoi
10.4067/S0034-98872013000900009subject
Has Abstractpub_date
2013-09-01 00:00:00pages
1166-72issue
9eissn
0034-9887issn
0717-6163pii
S0034-98872013000900009journal_volume
141pub_type
杂志文章abstract::Malignant Melanoma (MM) incidence rate is increasing faster than many other cancer at present time. Its epidemiological and clinical behavior varies within countries. We have studied 113 cases of cutaneous melanoma from 1982-1993, that represents until now the largest series of MM studied in Chile. Superficial Spreadi...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Verapamil is a calcium channel blocker ideally suited for the treatment of A-V nodal reciprocating supraventricular tachycardia. Most types of ventricular tachycardia are refractory to treatment with verapamil since they depend on sodium channels. However, a few cases of recurrent ventricular tachycardia are refractor...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::Currently, the Ebola virus has been classified by the WHO as "an international public health emergency" that has highlighted among other things, the enormous importance of proper and timely medical care. However, the complexity of this new health scenario, mainly due to personal risk in the event of infection, means t...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872015000700014
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:The Latin American Study of Congenital Malformations (ECLAMC) has performed an epidemiological surveillance of congenital malformations since 1967. This allows to detect any unexpected change in the incidence of malformations, possibly caused by a new environmental teratogenic agent. AIM:To report a summary...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872011000100010
更新日期:2011-01-01 00:00:00
abstract::A revolution is taking place in Medicine as a consequence of advances in biotechnology and informatics. The unraveling of the human genome with a description of chromosomal maps for all structural genes affords a new framework for the understanding of etiology, diagnosis and prevention of every disease. More than thre...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:
更新日期:1989-05-01 00:00:00
abstract::The earth of ornamental plants is one of the main reservoirs of Aspergillus type of fungi in hospital areas. We studied 174 ornamental interior plants from a hospital at Santiago. Samples were obtained from the soil surface and sowed in Sabouraud-glucose agar, adding streptomycin and G-penicillin. After 72 h of cultur...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract:BACKGROUND:New vaccination strategies are needed to control the increasing problem of pertussis in teenagers and adults. AIM:To determine the immunogenicity and reactogenicity of a diphtheria-tetanus-acellular pertussis (dTpa) vaccine with reduced antigen content. MATERIAL AND METHODS:A single dose of the dTpa vaccin...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:The six minute walk test (6MWT) is an important physical performance measure used in older people. AIM:To assess the total distance walked and the physiological cost of the 6MWT in non-disabled older people. MATERIAL AND METHODS:Fifty six females aged 69 ± 5 years with a body mass index (BMI) of 31 ± 4 kg/...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872015000400010
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:The Chilean Ministry of Health developed a healthy lifestyles intervention directed to adults with overweight and cardiovascular risk factors, called "Program on Healthy Eating and Physical Activity" (PASAF). AIM:To evaluate the impact of PASAF on nutritional status and metabolic parameters. PATIENTS AND M...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872014000700001
更新日期:2014-07-01 00:00:00
abstract::We report a 34-years-old male, with a history of hepatitis B and human immunodeficiency virus (HIV) infection that was admitted to the hospital with malaise, weight loss, frontal behavior and chest pain. Imaging studies showed two frontal cortical/subcortical nodules. A stereotactic cerebral biopsy showed reactive gli...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872011000200012
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:Free-text imposes a challenge in health data analysis since the lack of structure makes the extraction and integration of information difficult, particularly in the case of massive data. An appropriate machine-interpretation of electronic health records in Chile can unleash knowledge contained in large volum...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872019001001229
更新日期:2019-10-01 00:00:00
abstract::Hematopoietic precursors transplantation is a therapeutic alternative for leukemia, some metabolic diseases and some immune deficiency syndromes. In its allogeneic variety leukemia eradication is based in the conditioning prior to transplantation and the allograñ effect against leukemia. Umbilical cord blood is an alt...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872009000800009
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:Although endemic goiter is an easily controlled chronic disease, it continues to be a serious global public health problem. AIM:To study iodine nutrition in school age children from different areas of Chile. SUBJECTS AND METHODS:Thyroid gland was palpated in 4181 school age children from Calama, Santiago, ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:The most common type of oral cancer is squamous cell carcinoma, which accounts for approximately 90% of all oral malignancies. Therefore the oral cancer problem primarily concerns the diagnosis, biology and management of squamous cell carcinoma (SCC). However, there are red and white lesions, that are consid...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Understanding how autonomous students are capable of regulating their own learning process is essential to develop self-directed teaching methods. AIM:To understand how self-directed medical students approach learning in medical schools at University of Concepción, Chile. MATERIAL AND METHODS:A qualitative...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872015001200011
更新日期:2015-12-01 00:00:00
abstract::We report a 56 years-old man presenting with chest pain with exercise, seven years after an orthotopic heart transplant. A coronary angiography showed an atherosclerotic lesion in the common left main coronary artery with more than 90% obstruction. The lesion was successfully treated with a transluminal angioplasty wi...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872007001200012
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:There is paucity of information about viral etiology of community acquired pneumonia in adults. AIM:To investigate the viral etiology of pneumonia among hospitalized patients. MATERIAL AND METHODS:All adults with pneumonia that were hospitalized were prospectively enrolled at Puerto Montt hospital. A micro...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872012000800003
更新日期:2012-08-01 00:00:00
abstract::Sickle cell anemia was a rare disease in Chile, especially in adults, however the recent immigration wave from Haiti is changing this scenario. We report a 29 year old black female from Haiti with a non-disclosed history of sickle cell anemia. She was transfused with two units of red blood cells, found unconscious and...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872018001101347
更新日期:2018-11-01 00:00:00
abstract::During colonial times the Royal University of San Felipe was founded in 1738. The first plan for medical studies was outlined in 1779. During the Independence, the National Institute created in 1813 took over higher education. The first Course of Medicine was dictated there in 1833. The University of San Felipe was re...
journal_title:Revista medica de Chile
pub_type: 传,历史文章,杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Vitamin K antagonist medications (VKA) are essential for the prevention of thromboembolic events, but their effectiveness is influenced by multiple factors, such as the type of medication chosen. AIM:To evaluate the efficacy in anticoagulant control of the bioequivalent and non-bioequivalent drugs of acenoc...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872020000901254
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Renal transplantation can be done in patients with neurogenic bladder and clean intermittent self catheterization maintains renal function AIM:To retrospective assess the results of renal transplantation in patients with neurogenic bladder. PATIENTS AND METHODS:The medical records of seven patients aged 10...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Down syndrome (DS) is associated with higher child mortality especially due to cardiac malformations. AIM:To describe the trend in Chilean infant mortality in DS in the period 1997-2013 as compared to the general population without DS. MATERIAL AND METHODS:Raw data on infant deaths were extracted from the ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872016001100009
更新日期:2016-11-01 00:00:00
abstract::An analysis of the interaction of evolutionary factors such as migration, mutation, selection random drift, and consanguinity upon the genome of Easter islanders is attempted. As many primitive populations, the Easter islanders genome has at least 2/3 of genetic loci in an isogenic state. The HLA system is reduced; ot...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:
更新日期:1989-06-01 00:00:00
abstract::We are reporting a 37 year old male with severe oligozoospermia and a history of infertility for thirteen years and surgery for severe unilateral varicocele. The hormonal levels for FSH, LH and T, and karyotype were within the normal range. Multiplex PCR revealed the presence of a de novo microdeletion in the azoosper...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::We report the case of a woman with rapidly progressive IgA nephropathy. She was treated with pulse methylprednisolone sodium succinate (1 g/d for three days), cyclophosphamide, dipyridamole and aspirin. The rapid progression of renal failure was apparently halted after 6 months. ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract:BACKGROUND:The determination of thyroid stimulating hormone (TSH) reference values is critical for the diagnosis of thyroid diseases. AIM:To explore and discuss different definitions to establish TSH reference values using a Chilean national survey sample. MATERIAL AND METHODS:The 2009-2010 Chilean National Health Su...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872013000100013
更新日期:2013-01-01 00:00:00
abstract:AIM:To measure the impact of day care attendance on lower respiratory tract infections in children under two years old in two urban settings with different degrees of air pollution. SUBJECTS AND METHODS:A 26 week follow up, during fall and winter of 1991 and 1992, was performed to 98 children (48 attending day care an...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:With the aim of contributing to he Healthy Goal 2010 of reducing significantly the prevalence of childhood obesity we developed and implemented during 2003 and 2004, a school-based obesity prevention intervention which included nutrition education and the promotion of physical activity. AIM:To report the re...
journal_title:Revista medica de Chile
pub_type: 杂志文章,多中心研究
doi:/S0034-98872008000100003
更新日期:2008-01-01 00:00:00
abstract::There is an urgent need to increase the awareness on the Joint Hyper mobility Syndrome QHS). This is a congenital and prevalent emergent condition that is frequently undiagnosed and that causes significant health problems. Besides recurrent muscular-skeletal problems and signs and symptoms derived from tissue fragilit...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:/S0034-98872009001100013
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Wiskott-Aldrich syndrome (WAS) is an X linked congenital disease that presents as eczema, thrombocytopenia and immune deficiency. The only curative procedure for this illness is hematopoietic stem cell transplant (HSCT), preferably from a healthy HLA identical sibling donor. Cord blood is becoming an excelle...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872007000700013
更新日期:2007-07-01 00:00:00