Abstract:
:Nuclear-enriched RNA-binding proteins (RBPs) are mainly involved in transcriptional regulation, which is a critical checkpoint to tune gene diversity and expression levels. We analyzed nuclear RBPs in human HCC tissues and matched normal control tissues. Based on the gene expression levels, PTBP3 was identified as top-ranked in the nuclei of HCC cells. HCC cell lines then were transfected with siRNAs or lentiviral vectors. PTBP3 promoted HCC cell proliferation and metastasis both in vitro and in vivo. RNA immunoprecipitation (RIP), fluorescence in situ hybridization (FISH) and qRT-PCR assays verified that PTBP3 protein recruited abundant lnc-NEAT1 splicing variants (NEAT1_1 and NEAT1_2) and pre-miR-612 (precursor of miR-612) in the nucleus. NEAT1_1, NEAT1_2 and miR-612 expression levels were determined by PTBP3. Correlational analyses revealed that PTBP3 was positively correlated with NEAT1, but it was inversely correlated with miR-612 in HCC. The P53/CCND1 and AKT2/EMT pathways were determined by NEAT1 and miR-612 respectively in HCC. The PTBP3high and NEAT1high/miR-612low patients had a shorter overall survival. Therefore, nuclear-enriched RBP, PTBP3, promotes HCC cell malignant growth and metastasis by regulating the balance of splicing variants (NEAT1_1, NEAT1_2 and miR-612) in HCC.
journal_name
Oncogenejournal_title
Oncogeneauthors
Yang X,Qu S,Wang L,Zhang H,Yang Z,Wang J,Dai B,Tao K,Shang R,Liu Z,Li X,Zhang Z,Xia C,Ma B,Liu W,Li H,Dou Kdoi
10.1038/s41388-018-0416-8subject
Has Abstractpub_date
2018-12-01 00:00:00pages
6399-6413issue
50eissn
0950-9232issn
1476-5594pii
10.1038/s41388-018-0416-8journal_volume
37pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::DNA double-strand breaks (DSBs), the most hazardous DNA lesions, may result in genomic instability, a hallmark of cancer cells. The main DSB repair pathways are non-homologous end joining (NHEJ) and homologous recombination (HR). In mammalian cells, NHEJ, which can lead to inaccurate repair, predominates. HR repair (H...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.516
更新日期:2012-07-26 00:00:00
abstract::Mesenchymal stromal cells (MSCs) are strongly immunosuppressive via producing nitric oxide (NO) and known to migrate into tumor sites to promote tumor growth, but the underlying mechanisms remain largely elusive. Here, we found that interferon alpha (IFNα)-secreting MSCs showed more dramatic inhibition effect on tumor...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.128
更新日期:2016-11-17 00:00:00
abstract::As tumors grow and invade beyond their homeostatic limits, the tumor cells are subjected to insufficient nutrient and oxygen supplies because of excessive demand for nutrition and oxygen, and insufficient vascularization. We therefore hypothesized that tolerance to nutrient deprivation as well as angiogenesis may be c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205737
更新日期:2002-09-05 00:00:00
abstract::The mammalian target of rapamycin (mTOR) is a highly conserved serine-threonine kinase activated in response to growth factors and nutrients. Because of frequent dysregulation of the mTOR signaling pathway in diverse human cancers, this kinase is a key therapeutic target. Redd1 is a negative regulator of mTOR, mediati...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.102
更新日期:2011-09-01 00:00:00
abstract::Growth factor receptor-mediated signal transduction has been implicated in conferring resistance to conventional chemotherapy on cancer cells. In this study, we delineated a pathway that involves HER2/PI-3K/Akt in mediating multidrug resistance in human breast cancer cells. We found that the cell lines that express bo...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206394
更新日期:2003-05-22 00:00:00
abstract::Drosophila tumor suppressor WARTS (Wts) is an evolutionally conserved serine / threonine kinase and participates in a signaling complex that regulates both proliferation and apoptosis to ensure the proper size and shape of the fly. Human counterparts of this complex have been found to be frequently downregulated or mu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208682
更新日期:2005-08-11 00:00:00
abstract::Cytokinesis is the final step of cell division. Increasing evidence suggests failure of cytokinesis might contribute to the development of cancer. Here, we demonstrate that the serologically defined colon cancer antigen-3 (SDCCAG3) forms a complex with PTPN13, a protein tyrosine phosphatase known to be involved in the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.485
更新日期:2013-09-26 00:00:00
abstract::Caveolin-1 (Cav1) is an integral membrane, scaffolding protein found in plasma membrane invaginations (caveolae). Cav1 regulates multiple cancer-associated processes. In breast cancer, a tumor suppressive role for Cav1 has been suggested; however, Cav1 is frequently overexpressed in aggressive breast cancer subtypes, ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.474
更新日期:2013-09-19 00:00:00
abstract::We have found that the differentiation inducer butyric acid causes the synthesis of a cellular protein(s) that mediates a rapid decline in the level of myc RNA in SW837, a cell line derived from a human adenocarcinoma of the rectum. This effect was dose-dependent and was maximal at 1 mM. Among the short chain fatty ac...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::BTG2, a p53-inducible antiproliferative gene, is stimulated in breast cancer cells by activation of nuclear factor kappa B (NF-kappaB). In rat mammary glands, BTG2 is expressed in epithelial cells and levels decreased during pregnancy and lactation but recovered during involution. Estrogen and progestin suppress BTG2 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208008
更新日期:2004-10-28 00:00:00
abstract::To ensure that their mRNAs are translated and that the viral proteins necessary for assembling the next generation of infectious progeny are produced, viruses must effectively seize control of the translational machinery within their host cells. In many cases, the ability to productively engage host translational comp...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1209053
更新日期:2005-11-21 00:00:00
abstract::DNA methylation alterations are now widely recognized as a contributing factor in human tumorigenesis. A significant number of tumor suppressor genes are transcriptionally silenced by promoter hypermethylation, and recent research implicates alterations in chromatin structure as the mechanistic basis for this repressi...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1205602
更新日期:2002-08-12 00:00:00
abstract::The products of the proto-oncogenes c-jun and c-fos are known to form a complex in vivo. Complex formation appears to stabilize protein-DNA interactions and is thought to play an important functional role in transcriptional regulation. Here we show that the viral Jun oncoprotein, which differs structurally from cellul...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::The inactivation of certain tumor suppressor genes is thought to play an important role in the genesis of a number of tumor types. For example, inactivation of the Retinoblastoma (Rb) tumor suppressor is frequently observed in a proportion of sporadic human breast cancers. While these studies suggest that inactivation...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201707
更新日期:1998-04-16 00:00:00
abstract::RAS is a small GTP binding protein mutated in approximately 30% human cancer. Despite its important role in the initiation and progression of human cancer, the underlying mechanism of RAS-induced human epithelial transformation remains elusive. In this study, we probe the cellular and molecular mechanisms of RAS-media...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208753
更新日期:2005-09-08 00:00:00
abstract::One of the key events in tumor initiation in mouse skin is mutational activation of the H-ras gene. Papillomas induced by the most carcinogenic environmental polycyclic aromatic hydrocarbon (PAH), dibenzo[a,l]pyrene (DB[a,l]P), in SENCAR mouse skin contain a specific H-ras codon 61 (CAA-->CTA) mutation. We describe he...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201853
更新日期:1998-06-18 00:00:00
abstract::Hypoxic microenvironment supports cancer stem cell survival, causes poor response to anticancer therapy and tumor recurrence. Inhibition of Notch-1 signaling in adenocarcinoma of the lung (ACL) cells causes apoptosis specifically under hypoxia. Here, we found that Akt-1 activation is a key mediator of Notch-1 pro-surv...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.7
更新日期:2010-04-29 00:00:00
abstract::Germline BAP1 mutations predispose to several cancers, in particular malignant mesothelioma. Mesothelioma is an aggressive malignancy generally associated with professional exposure to asbestos. However, to date, we found that none of the mesothelioma patients carrying germline BAP1 mutations were professionally expos...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.243
更新日期:2016-04-14 00:00:00
abstract::Cell lines derived from cervical neoplasias, as well as cells from normal cervix and neonatal foreskin were examined in an in vitro culture system (raft system) that allows for stratification and differentiation of epithelial cells at an air-liquid interface. Epithelial cells from human foreskin and ectocervix were ob...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Predisposition to melanoma is genetically heterogeneous. Two high penetrance susceptibility genes, CDKN2A and CDK4, have so far been identified and mapping is ongoing to localize and identify others. With the advent of a catalogue of millions of potential DNA polymorphisms, attention is now also being focused on ident...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1206445
更新日期:2003-05-19 00:00:00
abstract::The onset of human lung cancer occurs through sequential mutations in oncogenes and tumor suppressor genes. Mutations in K-Ras play a prominent role in human non-small cell lung cancer. We have developed a mouse lung tumor model in which K-Ras can be sporadically activated through Cre-lox mediated somatic recombinatio...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204837
更新日期:2001-10-04 00:00:00
abstract::Max is a small helix-loop-helix protein which forms heterodimers with members of the Myc protein family. Myc/Max heterodimers exhibit sequence-specific DNA binding with much greater affinity than Myc homodimers. The Xenopus laevis homologue of Max, XMax, is shorter than the equivalent mammalian protein. This differenc...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Defects in a developmental signaling pathway involving the mammalian homologue of the Drosophila segment polarity gene, patched are associated with human tumors such as basal cell carcinoma, medulloblastoma and squamous cell carcinoma. Loss of heterozygosity (LOH) in some of these tumor cells suggests that patched fun...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205370
更新日期:2002-04-18 00:00:00
abstract::The heterogeneous breast cancers can be classified into different subtypes according to their histopathological characteristics and molecular signatures. Foxa1 expression is linked with luminal breast cancer (LBC) with good prognosis, whereas Twist1 expression is associated with basal-like breast cancer (BLBC) with po...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.286
更新日期:2017-02-23 00:00:00
abstract::Colitis-associated colorectal cancers are an etiologically distinct subgroup of colon cancers that occur in individuals suffering from inflammatory bowel disease and arise as a consequence of persistent exposure of hyperproliferative epithelial stem cells to an inflammatory microenvironment. An intrinsic defect in the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.545
更新日期:2012-08-09 00:00:00
abstract::The NF2 gene product, merlin/schwannomin, is a cytoskeleton organizer with unique growth-inhibiting activity in specific cell types. A narrow spectrum of tumors is associated with NF2 deficiency, mainly schwannomas and meningiomas, suggesting cell-specific mechanisms of growth control. We have investigated merlin func...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.427
更新日期:2009-02-12 00:00:00
abstract::Akt/PKB is a serine/threonine kinase that promotes tumor cell growth by phosphorylating transcription factors and cell cycle proteins. There is particular interest in finding tumor-specific substrates for Akt to understand how this protein functions in cancer and to provide new avenues for therapeutic targeting. Our l...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208590
更新日期:2005-06-16 00:00:00
abstract::Myc promotes both normal cell proliferation and oncogenic transformation through the activation and repression of target genes. The c-Myc-S protein is a truncated form of c-Myc that is produced in some cells from translation initiation at an internal AUG codon. We report that c-Myc-S and a similar truncated form of N-...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210734
更新日期:2008-02-21 00:00:00
abstract::Human T-cell leukemia virus type I (HTLV-I) causes adult T-cell leukemia (ATL) in about 5% of carriers after a long latent period. After its infection, HTLV-I promotes the clonal proliferation of HTLV-I infected cells in vivo by actions of encoded viral proteins, including Tax. However, leukemic cells frequently lack ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1206551
更新日期:2003-08-11 00:00:00
abstract::The role of specificity protein 1 (Sp1) in controlling gene expression in lung tumor development and metastasis is not well understood. In this study, we showed that the Sp1 level was highly increased and required for lung tumor growth in transgenic mice bearing Kras-induced lung tumors under the control of doxycyclin...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.568
更新日期:2012-08-30 00:00:00