Abstract:
:The understanding of the early mammalian development is a prerequisite for the advancement of in vitro fertilization and improvement of derivation and culturing of embryonic stem cells. While, whole genome transcriptomic analysis on bovine blastocysts has identified genes active in early development, little information is available about the protein complement of early embryos. Modern, sensitive proteomic technology (nano HPLC tandem mass spectrometry) allowed us to describe the proteome of the scarce blastocoel fluid and cell material of expanded bovine blastocysts isolated by micromanipulation. From two independent replicates, 23 proteins were identified in the blastocoel fluid while 803 proteins were identified in the remaining cell material. The proteins were grouped into categories according to their gene ontology (GO) terms by which proteins involved in cell differentiation, cell proliferation, development, and reproduction could be derived. Proteins classified in these categories could be candidates for further functional studies to understand pluripotency and early mammalian development.
journal_name
Syst Biol Reprod Medjournal_title
Systems biology in reproductive medicineauthors
Jensen PL,Grøndahl ML,Beck HC,Petersen J,Stroebech L,Christensen ST,Yding Andersen Cdoi
10.3109/19396368.2014.894152subject
Has Abstractpub_date
2014-06-01 00:00:00pages
127-35issue
3eissn
1939-6368issn
1939-6376journal_volume
60pub_type
杂志文章abstract::For natural fertilization to occur successfully, millions of spermatozoa must be deposited in the lower end of the female genital tract during mating. Considerably fewer spermatozoa are required for fertilization when spermatozoa are deposited in the upper region of the female tract. The extreme case of assisted ferti...
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