Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants.

Abstract:

:REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p<0.01). More specifically, seven single-copied T. atroviride T23 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants.

journal_name

Chemosphere

journal_title

Chemosphere

authors

He X,Wubie AJ,Diao Q,Li W,Xue F,Guo Z,Zhou T,Xu S

doi

10.1016/j.chemosphere.2014.01.023

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

526-30

eissn

0045-6535

issn

1879-1298

pii

S0045-6535(14)00089-7

journal_volume

112

pub_type

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