Cytotoxicity of human beta-interferon for differentiating leukemic HL-60 cells.

Abstract:

:We examined the effect of human interferon (HuIFN) -alpha and -beta on the proliferation and differentiation induced by dimethyl sulfoxide (DMSO) of HL-60 human promyelocytic leukemia cells into mature granulocytes. Neither HuIFN-alpha nor -beta, alone, from 1 to 1000 IU/ml, nor the homologous mock HuIFN preparations affected HL-60 cell differentiation or proliferation. Whereas the combination of HuIFN-alpha (10 to 1000 IU/ml) with DMSO also did not affect the proliferation or differentiation of HL-60 cells, the addition of HuIFN-beta (1000 IU/ml) and DMSO (1.25%) to growing cultures reduced cell viability as much as 14% of that observed for cells treated with DMSO alone or to 4% of that observed for either untreated cells or those treated with HuIFN-beta alone. The cytotoxic effect declined with decreasing concentrations of HuIFN-beta. The cytotoxic effect of DMSO and HuIFN-beta was exerted only as cells began to differentiate. Removal of HuIFN-beta at Day 2 did not reverse the cytotoxic effect, and addition of HuIFN-beta at Day 2 did not inhibit cell proliferation. Addition of HuIFN-beta to postmitotic cells on Day 4 after DMSO treatment did not affect proliferation but did slow differentiation. The cytotoxic and antidifferentiative effects of naturally produced HuIFN-beta were confirmed with highly purified recombinant HuIFN-beta. Undifferentiated HL-60 cells were resistant to the antiviral effects of HuIFN-beta, requiring 4 to 6 times the concentration to protect 50% of the cells against vesicular stomatitis virus as that needed to produce a cytotoxic or antidifferentiative effect. The profoundly cytotoxic effects of HuIFN-beta reported here may provide a model to study this interferon in combination with inducers of leukemic cell differentiation as a possible strategy in cancer therapy.

journal_name

Cancer Res

journal_title

Cancer research

authors

Hamburger AW,White CP,Siebenlist RE,Sedmak JJ,Grossberg SE

subject

Has Abstract

pub_date

1985-11-01 00:00:00

pages

5369-73

issue

11 Pt 1

eissn

0008-5472

issn

1538-7445

journal_volume

45

pub_type

杂志文章
  • Inactivation of the p53 tumor suppressor gene via a novel Alu rearrangement.

    abstract::Inactivation of the p53 tumor suppressor gene is a common finding in human cancer. In most cases, inactivation is due to a point mutation in the gene, but rearrangement of the p53 gene is sometimes observed. We analyzed the inactivation of p53 in the human pancreas cancer cell line Hs766T, which harbors a structural a...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Slebos RJ,Resnick MA,Taylor JA

    更新日期:1998-12-01 00:00:00

  • Subtype-Specific Tumor-Associated Fibroblasts Contribute to the Pathogenesis of Uterine Leiomyoma.

    abstract::Recent genomic studies have identified subtypes of uterine leiomyoma (LM) with distinctive genetic alterations. Here, we report the elucidation of the biological characteristics of the two most prevalent uterine leiomyoma subtypes, MED12-mutant (MED12-LM) and HMGA2-overexpressing (HMGA2-LM) uterine leiomyomas. Because...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-17-1744

    authors: Wu X,Serna VA,Thomas J,Qiang W,Blumenfeld ML,Kurita T

    更新日期:2017-12-15 00:00:00

  • High levels of dioxin-like potential in cigarette smoke evidenced by in vitro and in vivo biosensing.

    abstract::Cigarette smoke contains low levels of agonists for the aryl hydrocarbon receptor (AhR; also called the dioxin receptor). However, little is understood about the whole potential of cigarette smoke for activating AhR. In this report, we evaluated the total "dioxin-like" activity of cigarette smoke using in vitro and in...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-4541

    authors: Kasai A,Hiramatsu N,Hayakawa K,Yao J,Maeda S,Kitamura M

    更新日期:2006-07-15 00:00:00

  • Activation of the tamoxifen derivative metabolite E to form DNA adducts: comparison with the adducts formed by microsomal activation of tamoxifen.

    abstract::(Z)-1,2-Diphenyl-1-(4-hydroxyphenyl)but-1-ene (metabolite E) has been detected in the plasma of patients treated with tamoxifen. We therefore investigated whether the cis/trans isomers of metabolite E can be activated to form DNA adducts detected by 32P postlabeling. Microsomal activation of metabolite E produced two ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Pongracz K,Pathak DN,Nakamura T,Burlingame AL,Bodell WJ

    更新日期:1995-07-15 00:00:00

  • Identification of melanoma inhibitory activity and other differentially expressed messenger RNAs in human melanoma cell lines with different metastatic capacity by messenger RNA differential display.

    abstract::The differential display technique was used to identify mRNAs differentially expressed in human melanoma cell lines with different metastatic capacity. We report the isolation of nine different clones, of which four were uniquely expressed in the highly metastatic human melanoma cell line MV3, whereas the other five c...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: van Groningen JJ,Bloemers HP,Swart GW

    更新日期:1995-12-15 00:00:00

  • Plant-derived human papillomavirus 16 E7 oncoprotein induces immune response and specific tumor protection.

    abstract::Vaccine strategies for treatment of human papillomavirus-induced cervical cancer are based on either the recombinant E7 fusion oncoprotein or E7 CTL peptides. The therapeutic potential of the E7-based vaccine depends on the use of different adjuvants. In this study, we describe for the first time the expression of the...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Franconi R,Di Bonito P,Dibello F,Accardi L,Muller A,Cirilli A,Simeone P,Donà MG,Venuti A,Giorgi C

    更新日期:2002-07-01 00:00:00

  • Lipid-dependent proliferation of pancreatic cancer cell lines.

    abstract::Capan 1, a human pancreatic cancer cell line, is routinely grown in 10% fetal calf serum (FCS). In order to characterize the factors needed for its proliferation, FCS was replaced by a synthetic serum (Ultroser G). For Capan 1 proliferation we found that Ultroser G was as efficient as FCS. A subfraction of Ultroser G ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Clerc P,Bensaadi N,Pradel P,Estival A,Clemente F,Vaysse N

    更新日期:1991-07-15 00:00:00

  • Simple biochemical method to assess progestin effects on human endometrial DNA synthesis and its application to endometrial carcinoma.

    abstract::Endometria of normal histology from postmenopausal women receiving either estrogen or estrogen plus a progestin have been analyzed for nuclear estradiol receptor, epithelial DNA synthesis, isocitric dehydrogenase, and estradiol dehydrogenase activities. Epithelial DNA synthesis correlated positively with nuclear estra...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: King RJ,Raju KS,Siddle NC,Taylor RW,Whitehead MI

    更新日期:1983-10-01 00:00:00

  • Induction of bcl-xL expression in mammary epithelial cells is glucocorticoid-dependent but not signal transducer and activator of transcription 5-dependent.

    abstract::In the present study, we examined the role of prolactin and glucocorticoids in regulating bcl-x transcription in mammary epithelial cells. We report that dexamethasone, but not prolactin, induced native bcl-x gene expression in a dose-dependent manner in HC11 cells and enhanced serum-starved HC11 cell survival. This e...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Schorr K,Furth PA

    更新日期:2000-11-01 00:00:00

  • Genetic variants in oxidative stress-related genes predict chemoresistance in primary breast cancer: a prospective observational study and validation.

    abstract::Chemotherapy response in patients with primary breast cancer is difficult to predict and the role of host genetic factors has not been thoroughly investigated. We hypothesized that polymorphisms in oxidative stress (OS)-related genes, including estrogen-quinone metabolizing enzymes NQO2 and GSTM1-5, may influence dise...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-2998

    authors: Yu KD,Huang AJ,Fan L,Li WF,Shao ZM

    更新日期:2012-01-15 00:00:00

  • Major alterations in the nucleotide structure of DNA in cancer of the female breast.

    abstract::DNA of invasive ductal carcinomas from five women was analyzed for structural alterations in the purine nucleotides using gas chromatography-mass spectrometry with selected ion monitoring. The results were compared to those for a normal DNA control. The carcinoma DNA showed dramatically higher concentrations of the ba...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Malins DC,Haimanot R

    更新日期:1991-10-01 00:00:00

  • Combined integrin phosphoproteomic analyses and small interfering RNA--based functional screening identify key regulators for cancer cell adhesion and migration.

    abstract::Integrins interact with extracellular matrix (ECM) and deliver intracellular signaling for cell proliferation, survival, and motility. During tumor metastasis, integrin-mediated cell adhesion to and migration on the ECM proteins are required for cancer cell survival and adaptation to the new microenvironment. Using st...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-2515

    authors: Chen Y,Lu B,Yang Q,Fearns C,Yates JR 3rd,Lee JD

    更新日期:2009-04-15 00:00:00

  • Metabolism and metabolic effects of 8-azainosine and 8-azaadenosine.

    abstract::8-Azainosine (8-aza-HR) is of interest because of its activity against experimental tumors. Metabolic studies in cell cultures were performed with 8-aza-HR and with the structurally related nucleoside, 8-azaadenosine (9-beta-D-ribofuranosyl-8-azaadenine) (8-aza-AR), which has a lower degree of antitumor activity than ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bennett LL Jr,Allan PW

    更新日期:1976-11-01 00:00:00

  • Depletion of adenosine diphosphate-ribosyl transferase activity in rat liver during exposure to N-2-acetylaminofluorene: effect of thiols.

    abstract::The exposure of rats to a feeding regimen containing N-2-acetylaminofluorene (2AAF) causes an accumulation of lesions on liver DNA and a progressive impairment in DNA repair capacity. We used the in vivo experimental model of Teebor and Becker (Cancer Res., 31:1-3, 1971) with the carcinogen given to rats during four c...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Cesarone CF,Scovassi AI,Scarabelli L,Izzo R,Orunesu M,Bertazzoni U

    更新日期:1988-07-01 00:00:00

  • bfl-1, a bcl-2 homologue, suppresses p53-induced apoptosis and exhibits potent cooperative transforming activity.

    abstract::The bcl-2 family of genes code for proteins that contain anti-apoptotic or pro-apoptotic activity. The human bfl-1 gene contains an open reading frame for a 175-amino acid Bcl-2 family protein. Among the various Bcl-2 family members, the Bfl-1 protein shares the highest homology with the mouse A1 protein. These two pr...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: D'Sa-Eipper C,Subramanian T,Chinnadurai G

    更新日期:1996-09-01 00:00:00

  • The c-Fes protein-tyrosine kinase suppresses cytokine-independent outgrowth of myeloid leukemia cells induced by Bcr-Abl.

    abstract::The c-Fes protein-tyrosine kinase exhibits strong expression in myeloid hematopoietic cells. Previous studies have shown that Fes induces differentiation in the chronic myelogenous leukemia-derived cell line K-562, suggesting that the Fes signal for differentiation is dominant to the Bcr-Abl signal for transformation ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Lionberger JM,Smithgall TE

    更新日期:2000-02-15 00:00:00

  • Regulation of matrix metalloproteinase genes by E2F transcription factors: Rb-Raf-1 interaction as a novel target for metastatic disease.

    abstract::The retinoblastoma (Rb)-E2F transcriptional regulatory pathway plays a major role in cell-cycle regulation, but its role in invasion and metastasis is less well understood. We find that many genes involved in the invasion of cancer cells, such as matrix metalloproteinases (MMP), have potential E2F-binding sites in the...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-2647

    authors: Johnson JL,Pillai S,Pernazza D,Sebti SM,Lawrence NJ,Chellappan SP

    更新日期:2012-01-15 00:00:00

  • Involvement of CPP32/Yama(-like) proteases in Fas-mediated apoptosis.

    abstract::Fas (Apo-1/CD95) belongs to the tumor necrosis factor/nerve growth factor receptor family and transmits apoptotic signals by binding to its ligand. Interleukin-1beta-converting enzyme (ICE), which shows substantial homology to the product of the cell death gene, ced-3, of Caenorhabditis elegans, is reported to be invo...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hasegawa J,Kamada S,Kamiike W,Shimizu S,Imazu T,Matsuda H,Tsujimoto Y

    更新日期:1996-04-15 00:00:00

  • Tumor-promoting phorbol ester and activated Ha-ras synergistically reduce the interleukin 3 requirement in a mast cell line.

    abstract::Infection of the bone marrow-derived mast cell line PB-3c with a retrovirus carrying oncogenic c-Ha-ras or v-Ha-ras reduced the interleukin 3 (IL-3) growth requirement and induced a state of tumorigenicity. In contrast, normal c-Ha-ras had no effect on the IL-3 requirement of this cell line nor did the cells become tu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Imber R,Fabbro D

    更新日期:1991-01-15 00:00:00

  • Multiple estrogen receptor assays in human breast cancer.

    abstract::A review of assay results from more than 6000 patients revealed 232 patients in whom multiple breast cancer specimens were analyzed for estrogen receptor (ER). All assays were performed in a single laboratory. Specimens were considered estrogen receptor positive (ER+) if the ER level was greater than 10 fmol/mg protei...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hull DF 3rd,Clark GM,Osborne CK,Chamness GC,Knight WA 3rd,McGuire WL

    更新日期:1983-01-01 00:00:00

  • Polyamine analogue induction of the p53-p21WAF1/CIP1-Rb pathway and G1 arrest in human melanoma cells.

    abstract::Although polyamines are well recognized for their critical involvement in cell growth, the cell cycle specificity of this requirement has not yet been characterized with respect to the newly delineated regulatory pathways. We recently reported that polyamine analogues having close structural and functional similaritie...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kramer DL,Vujcic S,Diegelman P,Alderfer J,Miller JT,Black JD,Bergeron RJ,Porter CW

    更新日期:1999-03-15 00:00:00

  • Concordant epigenetic silencing of transforming growth factor-beta signaling pathway genes occurs early in breast carcinogenesis.

    abstract::Human mammary epithelial cells (HMEC) grown under standard cell culture conditions enter a growth phase referred to as selection, but a subpopulation is able to escape from arrest and continue to proliferate. These cells, called post-selection or variant HMECs, may be derived from progenitor cells found in normal mamm...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-1284

    authors: Hinshelwood RA,Huschtscha LI,Melki J,Stirzaker C,Abdipranoto A,Vissel B,Ravasi T,Wells CA,Hume DA,Reddel RR,Clark SJ

    更新日期:2007-12-15 00:00:00

  • Activation of human long interspersed nuclear element 1 retrotransposition by benzo(a)pyrene, an ubiquitous environmental carcinogen.

    abstract::Long interspersed nuclear elements [LINE-1 (L1)] are abundant retrotransposons in mammalian genomes that remain silent under most conditions. Cellular stress signals activate L1, but the molecular mechanisms controlling L1 activation remain unclear. Evidence is presented here that benzo(a)pyrene (BaP), an environmenta...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-3478

    authors: Stribinskis V,Ramos KS

    更新日期:2006-03-01 00:00:00

  • Human in vivo radiation-induced biomarkers: gene expression changes in radiotherapy patients.

    abstract::After initially identifying potential biomarkers of radiation exposure through microarray studies of ex vivo irradiated human peripheral white blood cells, we have now measured the in vivo responses of several of these biomarker genes in patients undergoing total body irradiation. Microarray analysis has identified ad...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-04-1883

    authors: Amundson SA,Grace MB,McLeland CB,Epperly MW,Yeager A,Zhan Q,Greenberger JS,Fornace AJ Jr

    更新日期:2004-09-15 00:00:00

  • Analysis of the inhibitory effects of VP-16-213 (etoposide) and podophyllotoxin on thymidine transport and metabolism in Ehrlich ascites tumor cells in vitro.

    abstract::These studies explore the effects of an epipodophyllotoxin on the membrane transport and metabolism of thymidine in Ehrlich ascites tumor cells. Uptake of 3H after exposure of cells to [3H]-thymidine is characterized by a rapid initial velocity that approximates membrane transport followed by a slower rate of uptake t...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Yalowich JC,Goldman ID

    更新日期:1984-03-01 00:00:00

  • Identification of a promiscuous T-cell epitope encoded by multiple members of the MAGE family.

    abstract::One of the major limitations of tumor-specific vaccination is the generation of antigen-loss variants that are able to escape the immune response elicited by a monoantigenic peptide epitope. Here, we report the identification of a new HLA-B*3701-restricted epitope shared by four different members of the MAGE family. P...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Tanzarella S,Russo V,Lionello I,Dalerba P,Rigatti D,Bordignon C,Traversari C

    更新日期:1999-06-01 00:00:00

  • Acidosis Acts through HSP90 in a PHD/VHL-Independent Manner to Promote HIF Function and Stem Cell Maintenance in Glioma.

    abstract::Hypoxia is a common feature of solid tumors, which controls multiple aspects of cancer progression. One important function of hypoxia and the hypoxia-inducible factors (HIF) is the maintenance of cancer stem-like cells (CSC), a population of tumor cells that possess stem cell-like properties and drives tumor growth. A...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-15-2630

    authors: Filatova A,Seidel S,Böğürcü N,Gräf S,Garvalov BK,Acker T

    更新日期:2016-10-01 00:00:00

  • c-myc amplification is a better prognostic factor than HER2/neu amplification in primary breast cancer.

    abstract::Amplification of the c-myc and HER2/neu genes was found in 20 and 23%, respectively, of primary breast cancer tissues derived from 282 patients (median follow-up, 74 months). c-myc amplification was observed more frequently in larger tumors (P = 0.01) and in lymph node-positive patients (P = 0.01) but was not associat...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Berns EM,Klijn JG,van Putten WL,van Staveren IL,Portengen H,Foekens JA

    更新日期:1992-03-01 00:00:00

  • Morphological and molecular heterogeneity within nonmicrosatellite instability-high colorectal cancer.

    abstract::Colorectal cancer (CRC) has traditionally been classified into two groups: microsatellite stable/low-level instability (MSS/MSI-L) and high-level MSI (MSI-H) groups on the basis of multiple molecular and clinicopathologic criteria. Using methylated in tumor (MINT) markers 1, 2, 12, and 31, we stratified 77 primary CRC...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Whitehall VL,Wynter CV,Walsh MD,Simms LA,Purdie D,Pandeya N,Young J,Meltzer SJ,Leggett BA,Jass JR

    更新日期:2002-11-01 00:00:00

  • Expression of vascular permeability factor/vascular endothelial growth factor by melanoma cells increases tumor growth, angiogenesis, and experimental metastasis.

    abstract::Vascular permeability factor (VPF)/vascular endothelial growth factor (VEGF) is an angiogenic cytokine expressed by many human and animal tumors. Hypoxia often up-regulates VPF/VEGF expression further. To better define the role of VPF/VEGF in tumor biology, we screened tumorigenic lines for those expressing minimal co...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Claffey KP,Brown LF,del Aguila LF,Tognazzi K,Yeo KT,Manseau EJ,Dvorak HF

    更新日期:1996-01-01 00:00:00