Abstract:
:Commonly used implants for therapeutic approaches of non-systemically impaired bone do not sufficiently support the healing process of osteoporotic bone. Since strontium (II) has been proven as an effective anti-osteoporotic drug new types of strontium enriched calcium phosphate bone cements were developed. As osteoporosis is characterized by an imbalance of osteoblast and osteoclast activity the influence of this newly generated strontium enriched biomaterials on the cellular behavior of osteoblast-like cells was investigated by time of flight secondary ion mass spectrometry (ToF-SIMS). ToF-SIMS is used to analyze whether strontium is incorporated in the mineralized extracellular matrix (mECM) and whether there is strontium uptake by osteogenically differentiated human mesenchymal stem cells (hMSCs). Therefore hMSCs were cultured in osteogenic differentiation medium for 21 days on two different strontium enriched bone cements (S100 and A10) and for reference also on the pure calcium phosphate cement (CPC) and on a silicon wafer. The distribution of strontium in the osteoblast-like cells and within their mineralized extracellular matrix was analyzed. A higher intensity of the strontium signal could be detected in the region of the mECM, synthesized by cells cultivated on the Sr- substituted bone cement (S100) in comparison to the reference groups. The osteoblast-like cells used the released strontium from the biomaterial to synthesize their mECM. Apart from that a uniform strontium distribution was measured within all investigated cells. However, different amounts of strontium were found in cells cultured on different biomaterials and substrates. Compared to the negative controls the strontium content in the cells on the strontium enriched biomaterials was much higher. A higher concentration of strontium inside the cells means that more strontium can take part in signaling pathways. As strontium is known for its beneficial effects on osteoblasts by promoting osteoblastic cell replication and differentiation, and reducing apoptosis, the newly developed strontium enriched calcium phosphate cements are promising implant materials for osteoporotic bone.
journal_name
Biointerphasesjournal_title
Biointerphasesauthors
Kokesch-Himmelreich J,Schumacher M,Rohnke M,Gelinsky M,Janek Jdoi
10.1186/1559-4106-8-17subject
Has Abstractpub_date
2013-12-01 00:00:00pages
17issue
1eissn
1934-8630issn
1559-4106journal_volume
8pub_type
杂志文章相关文献
Biointerphases文献大全abstract::The aim of this research was to assess the influence of mechanical loading on the ability of Zn-free versus Zn-containing amalgams to promote remineralization at the dentin interface. Sound and caries-affected dentin surfaces (CAD) were restored using Zn-free or Zn-containing dental amalgams. Midcoronal dentin surface...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4933243
更新日期:2015-12-19 00:00:00
abstract::Photocorrosion of semiconductors is strongly sensitive to the presence of surface states, and it could be influenced by electrically charged molecules immobilized near the semiconductor/electrolyte interface. The underlying mechanism is related to band bending of the semiconductor structure near the surface and the as...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4941983
更新日期:2016-03-22 00:00:00
abstract::Surface plasmon resonance immunosensor for the detection of bacterial cells was first reported in 1998 with high detection limit as much as 107 cfu/ml. Since then, many efforts have been made aiming to lower the detection limit and improve the sensitivity of detection. The aim of this study was to compare the effect o...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4971270
更新日期:2016-12-06 00:00:00
abstract::The mechanical properties and responses of cells to external stimuli (including drugs) are closely connected to important phenomena such as cell spreading, motility, activity, and potentially even differentiation. Here, reversible changes in the viscoelastic properties of surface-attached fibroblasts were induced by t...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0043-9
更新日期:2012-12-01 00:00:00
abstract::With the advent of novel fabrication technologies, free-standing poly(l-lactic acid) (PLLA) nanosheets have been shown to have enhanced performance over their micro- or macroscale equivalents as tissue engineering and drug delivery constructs. In the present research, the authors investigated the surface degradation b...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4908206
更新日期:2015-03-20 00:00:00
abstract::A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to mater...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000511
更新日期:2020-12-18 00:00:00
abstract::The surface concentration gradient of two extracellular matrix (ECM) macromolecules was developed to study the migratory and morphological responses of astrocytes to molecular cues typically found in the central nervous system injury environment. The gradient, prepared using microcontact printing, was composed of rand...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5001675
更新日期:2017-09-11 00:00:00
abstract::Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous in...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-011-0017-3
更新日期:2012-12-01 00:00:00
abstract::As an antibiotic that prevents and treats infections caused by Gram-positive bacteria such as Staphylococcus aureus, vancomycin incorporated in a biodegradable polymer poly(lactide-co-glycolide) provides opportunities to construct controlled-release drug delivery systems. Developments associated with this promising sy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5016186
更新日期:2018-01-10 00:00:00
abstract::Photolithographic techniques have been used to fabricate polymer brush micro- and nanostructures. On exposure to UV light with a wavelength of 244 nm, halogens were selectively removed from films of chloromethylphenyltrichlorosilane and 3-(2-bromoisobutyramido)propyl-triethoxysilane on silicon dioxide. Patterning was ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3553579
更新日期:2011-03-01 00:00:00
abstract::Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003708
更新日期:2017-12-05 00:00:00
abstract::Cationic amphiphilic peptides have been engineered to target both Gram-positive and Gram-negative bacteria while avoiding damage to other cell types. However, the exact mechanism of how these peptides target, bind, and disrupt bacterial cell membranes is not understood. One specific peptide that has been engineered to...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4982710
更新日期:2017-05-05 00:00:00
abstract::Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2710336
更新日期:2007-03-01 00:00:00
abstract::Coatings of biomaterials or implants that facilitate biomineralization possess a great potential for applications focused to the replacement, augmentation, and regeneration of bone tissue. Biomimetic approaches utilize biomolecules for either templating or supporting the crystallization process. One of these promising...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3589176
更新日期:2011-06-01 00:00:00
abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4927679
更新日期:2015-09-05 00:00:00
abstract::The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replic...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0036-8
更新日期:2012-12-01 00:00:00
abstract::Reliable, direct "sample-to-answer" capture of nucleic acid targets from complex media would greatly improve existing capabilities of DNA microarrays and biosensors. This goal has proven elusive for many current nucleic acid detection technologies attempting to produce assay results directly from complex real-world sa...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2219110
更新日期:2006-06-01 00:00:00
abstract::The structure, orientation, and formation of amphiphilic alpha-helix model peptide films on fluorocarbon surfaces has been monitored with sum frequency generation (SFG) vibrational spectroscopy, near-edge x-ray absorption fine structure (NEXAFS) spectroscopy, and x-ray photoelectron spectroscopy (XPS). The alpha-helix...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3317116
更新日期:2010-03-01 00:00:00
abstract::Shewanella oneidensis MR-1 is a dissimilatory metal-reducing bacterium capable of reducing various metal and sulfur compounds and precipitating them in nanoparticulate form. Here, we report the synthesis of molybdenum disulfide nanomaterials at the site of S. oneidensis biofilms grown in the presence of molybdenum tri...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000199
更新日期:2020-07-24 00:00:00
abstract::Gold nanoparticles (AuNPs) functionalized with a short chain amine-terminated alkanethiol (HS-(CH(2))(2)NH(2) or C2 NH(2)-thiol) are prepared via a direct synthesis method and then ligand-exchanged with a long chain amine-terminated alkanethiol (HS-(CH(2))(11)NH(2) or C11 NH(2)-thiol). Transmission electron microscopy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3622481
更新日期:2011-09-01 00:00:00
abstract::The kinetics of adsorption and desorption of two highly asymmetrical model peptides were studied at methyl- and carboxylic acid-terminated alkylthiolate self-assembled monolayer (SAM) surfaces on gold. The model peptides were leucine-lysine (LK), α-helical (LKα14), and β-strand (LKβ15) peptides that have a well-define...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3494080
更新日期:2010-12-01 00:00:00
abstract::Many studies have examined the effects of surface topography on the settlement behaviour of marine organisms and this article reviews these investigations with more emphasis on the effects of topography scale. It has been observed that macro topographies (1-100 mm) are generally favoured by marine fouling taxa and are...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1186/1559-4106-8-30
更新日期:2013-12-01 00:00:00
abstract::Neuronal process growth is guided by extrinsic environmental cues such as extracellular matrix (ECM) proteins. Recent reports have described that the growth cone extension is superior across gradients of the ECM protein laminin compared to growth across uniformly distributed laminin. In this work, the authors have pre...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4857295
更新日期:2014-03-01 00:00:00
abstract::It has been demonstrated that electric fields can minimize surface adhesion of bacteria, but how they affect the near-surface bacterial dynamics has remained largely overlooked. In the present study, the three-dimensional motions of Escherichia coli (E. coli) HCB1 and HCB1414 near a conductive surface under alternatin...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5078543
更新日期:2019-02-20 00:00:00
abstract::The articular cartilage, once injured, has a limited capacity for intrinsic repair. Preparation of functionally biocartilage substitutes in vitro for cartilage repair is an attractive concept with the recent advances in tissue engineering. In this study, adipose-derived adult stem cells (ADAS) and chondrocytes (Ch) we...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4942754
更新日期:2016-03-25 00:00:00
abstract::The growing interest in artificial bioorganic interfaces as a platform for applications in emerging areas as personalized medicine, clinical diagnostics, biosensing, biofilms, prevention of biofouling, and other fields of bioengineering is the origin of a need for in detail multitechnique characterizations of such lay...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4948341
更新日期:2016-06-02 00:00:00
abstract::Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extrace...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4994299
更新日期:2017-09-28 00:00:00
abstract::In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium pl...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0027-9
更新日期:2012-12-01 00:00:00
abstract::Simulations of water at hydrophilic self-assembled monolayer (SAM) surfaces are especially relevant for biological interfaces. Well-defined, atomically smooth surfaces that can be continuously varied are possible with SAMs. These characteristics enable more accurate measurements than many other surfaces with the added...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2977751
更新日期:2008-09-01 00:00:00
abstract::This study presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reducti...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4941367
更新日期:2016-03-12 00:00:00