Abstract：
OBJECTIVE:To investigate the role of microRNAs (miRNAs) and its mechanism in osteoblast mineralization. MATERIALS AND METHODS:Real-time polymerase chain reaction (PCR), Northern Blot, and Western Blot were used to identify the expression mode of regulators. Overexpression and down-regulation experiments were carried out to study the role of miR-98 and interactions between regulators. Bioinformatics calculation and luciferase reporter assay were used to prove the target gene. Electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (CHIP), and promoter luciferase reporter assay confirmed the relationship between the regulator and the promoter of miR-98. RESULTS:MiR-98 was up-regulated during osteoblast mineralization. Overexpression of miR-98 promoted osteoblast mineralization. Factor inhibiting activating transcription factor 4 (ATF4)-mediated transcription (FIAT), a negative regulator of osteoblast differentiation, was confirmed to be a target of miR-98. As a motivator in osteoblast mineralization, Sp7 transcription factor 7 (Sp7) promoted miR-98 transcription by a combination on the promoter region. CONCLUSIONS:Our study showed that miR-98 was an important regulator in osteoblast mineralization and miR-98 carried out its function through a novel miR-98-FIAT/Sp7 regulatory loop. It provides new insights into the roles of miRNAs in osteoblast mineralization.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Yang L,Huang LY,Li LB,Tang Z,Chen K,Chen LP,Luo HY,Luo J,Zhao XLdoi
10.26355/eurrev_201806_15246subject
Has Abstractpub_date
2018-06-01 00:00:00pages
3676-3688issue
12eissn
1128-3602issn
2284-0729journal_volume
22pub_type
杂志文章abstract：BACKGROUND AND AIM:Inflammatory cells involved in the pathophysiology of asthma express nicotinic receptor. Therefore 1,1 dimethyl(-4-)phenylpiperazinium (DMPP) in two doses were compared to dexamethasone in asthmatic guinea pigs. MATERIALS AND METHODS:Six groups were included; Normal control and five asthmatic (OVA-s...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Traumatic brain injury (TBI) is a serious neurodisorder commonly caused by sports related events or violence. It is the leading cause of disability in people under 40. AIM:In order to elucidate the molecular mechanism of the secondary injury after TBI. MATERIALS AND METHODS:In this study, we downloaded gen...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:Hemorrhagic shock is a common vital condition in obstetrics, and major treatment consists of bleeding control and liquid resuscitation. MicroRNA (miR) has been found to regulate multiple diseases. However, its expression profile in hemorrhagic shock or effects on the ischemia-reperfusion injury in pregnant mi...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201803_14600
更新日期：2018-03-01 00:00:00
abstract：:We have studied the temporal bones of 4 deceased donors, individuals one with cochlear saccular degeneration, another with Mondini dysplasia, another with an ossification of the basal turn of the cochlea and the round window, post meningitis and the fourth who was implanted 10 years before. The indications and contrai...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:The diagnosis of spondylodiscitis can be difficult, because the patients history, subjective symptoms and physical findings are often inconclusive, particularly in the early stages. AIM:To perform an overview on the role of nuclear medicine procedures with single photon emission tomography (SPET) and positr...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2012-04-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the effects of miR-940 and Toll-like receptor 4/Nuclear Factor κB (TLR4/NF-κB) pathways on inflammatory responses and spinal cord injury (SCI). MATERIALS AND METHODS:This study first established a model of spinal cord injury in mice. The grip force measurement was use...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17677
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVES:Echinococcosis infection is caused in humans by the larval stage of cestodes belonging to the genus Echinococcus. Hydatid cyst cured by percutaneous aspiration, infusion of scolicidal agents with reaspiration or surgery. Many scolicidal agents have been used for inactivation of the cyst's content, but most o...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVES:To demonstrate the pure effect of obstructive sleep apnea syndrome (OSAS) on cardiac autonomic function (CAF) using heart rate turbulence (HRT) parameters. PATIENTS AND METHODS:A total of 64 patients with OSAS and 30 age- and gender-matched healthy subjects were enrolled. All subjects had normal coronary ar...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:The precise etiology of inflammatory bowel diseases (IBDs) is still unknown although dysregulation of apoptosis likely plays an important role in this pathogenesis. However, the significance of mucosal T-cell apoptosis in ulcerative colitis (UC) is unclear. In the present work we investigated the role of TNF-...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:This study aims to observe the influence of 1,25-(OH)2D3 on airway inflammation and chemokine expression in asthmatic rats and to explore its significance in the treatment of asthma. MATERIALS AND METHODS:Wistar rats were randomly divided into a normal control group (N), an asthma group (A), a 1,25-(OH)2D3 g...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to determine characteristics of non-motor symptoms (NMS) in early Parkinson's disease (PD) with olfactory deficits and to analyze the feasibility of diagnosing PD based on olfactory deficits. PATIENTS AND METHODS:62 patients without olfactory deficits in early PD (PD group), 58 patie...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to detect the relative expression level of micro-ribonucleic acid (miR)-769-5p in gastric cancer (GC) tissues and cells, and to investigate the clinical significance, biological function, and mechanism of miR-769-5p in GC. PATIENTS AND METHODS:The relative expression level in 62...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202006_21657
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:Our goal was to evaluate the long-term clinical outcome of ankle arthrodesis, obtained by an extramedullary internal fixation with or without bone-grafting in the treatment of ankle' septic arthritis. PATIENTS AND METHODS:All patients treated with arthrodesis by extramedullary internal fixation for septic ar...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17483
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:The present study aimed to explore the role of long non-coding RNA NEAT1 (NEAT1) in mediating non-small cell lung cancer (NSCLC) cell migration and invasion, as well as the underlying regulatory mechanisms. PATIENTS AND METHODS:The NEAT1 expression in NSCLC tissues and cell lines was measured using reverse t...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-02-01 00:00:00
abstract：AIM OF STUDY:To evaluate the prevalence of latex allergy in a population of children with spina bifida (SB) and to assess the role of early exposure to latex products and others risk factors. INTRODUCTION:SB is related with an higher incidence of latex allergic reactions. These patients received repeated surgical proc...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVE:To investigate the expression of Long non-coding RNA ADIPOQ and its facilitating effects on proliferation and invasion of colorectal cancer by modulating the expression of TP53 via sponging with miR-219c-3p. PATIENTS AND METHODS:qRT-PCR was performed to detect the expressions of ADIPOQ and TP53 in human colo...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202007_22265
更新日期：2020-07-01 00:00:00
abstract：:The article "Evaluation of cytotoxicity of some common ophthalmic drugs" by M. Li, X.-M. Chen, J.-J. Liu, D.-M. Wang, L. Gan, X. Lv, Y. Qiao, published in Eur Rev Med Pharmacol Sci 2015; 19 (11): 1945-1950 has been withdrawn. ...
journal_title：European review for medical and pharmacological sciences
pub_type： 撤回出版物
doi：
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of Lactobacillus rhamnosus GR-1 on atherosclerotic progression in apolipoprotein-E knockout (ApoE-/-) mice fed with a high-fat diet and the underlying mechanisms of its action. MATERIALS AND METHODS:Eight-week-old ApoE-/- mice were treated with Lactobacillus rhamnosus GR-1 daily for...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17722
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:To investigate the relative expression of long non-coding RNA 00673 (lncRNA 00673) in hepatocellular carcinoma (HCC) and HCC cells and study its regulation on the malignant phenotype of HCC cells PATIENTS AND METHODS: Samples of HCC and adjacent tissues from January 2013 to December 2015 were collected. The e...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVE:Lercanidipine is a calcium antagonist with no cardiodepressant activity, long lasting antihypertensive action and reno-protective effect. Our previous data demonstrated that lercanidipine blocks L-type calcium channels (CaL). However, no data are available concerning its effects on T-type calcium channels (Ca...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201806_15289
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the expression level of microRNA-21-5p and its target gene PLAG1 in the serum of patients with spinal cord injury (SCI), as well as the role and mechanism of microRNA-21-5p in lipopolysaccharide-induced primary microglial inflammation. PATIENTS AND METHODS:The levels ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202006_21480
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:This study aims to investigate whether PM2.5 exposure is involved in the induction of alveolar epithelial cell apoptosis and the progression of emphysema in mice, and to further explore its specific molecular mechanism. MATERIALS AND METHODS:A certain number of PM2.5 exposed mice and normal control mice were...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202004_20863
更新日期：2020-04-01 00:00:00
abstract：OBJECTIVE:The objective of this work is to compare cellular toxicity in vitro of two resins for orthodontic use: an auto-polymerizable composite and a photo-polymerizable composite. MATERIALS AND METHODS:Samples were obtained by joining a couple of steel orthodontic brackets by using auto-polymerizing or photo-polymer...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202001_20078
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:Dynamic pupillometry (DP) is a simple, non-invasive computerized assessment of pupillary light response which provides data concerning both branches of the autonomous nervous system (ANS). Heart rate variability (HRV) analysis assess cardiac health and the ANS modulation on the heart. In this study, we aimed ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:The etiology of Major depressive disorder (MDD) is multifactorial but the genetic risk is an important factor. Previous studies have shown a significant interaction between serotonin and brain-derived neurotrophic factor (BDNF) in brain function. The serotonin transporter protein promoter polymorphism (5-HTTL...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to investigate the influences of micro ribonucleic acid (miR)-21 and downstream Toll-like receptor 4 (TLR4)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway on myocardial apoptosis induced by myocardial ischemia-reperfusion (I/R) injury in rats. MATERIAL...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201811_16420
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:This study was aimed to explore the role of long noncoding RNA (lncRNA) AB073614 and its potential mechanisms in the glioma development. MATERIALS AND METHODS:Expression levels of lncRNA AB073614 in glioma tissues and glioma cell lines were evaluated by quantitative real-time PCR (qRT-PCR). MTT assays were c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：:Many factors have been identified to influence the risk of mother to child transmission (MTCT) of HIV. Chief amongst these is high maternal VL and advanced disease. High maternal viral load (mVL), measured at delivery, has been described as the strongest risk factor for both in utero (IU) and intrapartum (IP) transmis...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:In this study, we explored the regulative effect of miR-195 on c-myb expression and also investigated the role of miR-195 and c-myb in cardiomyocyte apoptosis induced by hypoxia/reoxygenation (H/R) injury. MATERIALS AND METHODS:QRT-PCR analysis was performed to measure mature miR-195 expression. H9c2 cells w...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：:Leukemia is a malignant disease of the blood system. Although the current research on the pathogenesis of leukemia is more and more in-depth and has an influential guiding significance for the ongoing clinical treatment of leukemia, its resistance and recurrence is still a challenging problem that needs to be solved. ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_201908_18523
更新日期：2019-08-01 00:00:00
