The influence of hypoxia on the differentiation capacities and immunosuppressive properties of clonal mouse mesenchymal stromal cell lines.

Abstract:

:Multipotent mesenchymal stromal cells are multipotent cells capable of differentiating into different mesodermal cell types. Enigmatically, mesenchymal stromal cells present in the bone marrow support early lymphopoiesis yet can inhibit mature lymphocyte growth. Critical features of the bone marrow microenvironment, such as the level of oxygen, play an important role in mesenchymal stromal cell biology. Herein, we show that a panel of continuously growing mouse mesenchymal stromal cell lines, namely OP9, MS5, PA6, ST2 and B16-14, exhibit mesenchymal stromal cell characteristic phenotypes and respond physiologically to oxygen deprivation. Culturing freshly isolated bone marrow-derived mesenchymal stromal cells or cell lines at 5% O2 resulted in a dramatic increase in expression of hypoxia-inducible factor family members and of key genes involved in their differentiation. Phenotypically, their osteogenic and adipogenic differentiation capacity was generally improved in hypoxia, whereas their inhibitory effects on in vitro T-cell proliferation were preserved. Taken together, we conclude that these continuously growing mouse cell lines behave as canonical mesenchymal stromal cells and respond physiologically to hypoxia, thereby providing a potent tool for the study of different aspects of mesenchymal stromal cell biology.

journal_name

Immunol Cell Biol

authors

Prado-Lòpez S,Duffy MM,Baustian C,Alagesan S,Hanley SA,Stocca A,Griffin MD,Ceredig R

doi

10.1038/icb.2014.30

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

612-23

issue

7

eissn

0818-9641

issn

1440-1711

pii

icb201430

journal_volume

92

pub_type

杂志文章
  • Suppressive effects of serum on the LPS-induced production of nitric oxide and TNF-alpha by a macrophage-like cell line, WEHI-3, are dependent on the structure of polysaccharide chains in LPS.

    abstract::The effect of serum on LPS-induced activation of a murine macrophage-like cell line, WEHI-3, was examined. Foetal calf serum strongly inhibited the production of nitric oxide (NO) and TNF-alpha by LPS-stimulated WEHI-3 cells, while it enhanced the production of both by other macrophage-like cell lines, J774.1 and BAM3...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1711.1999.00807.x

    authors: Ohki K,Amano F,Yamamoto S,Kohashi O

    更新日期:1999-04-01 00:00:00

  • Activating and inhibitory receptors of natural killer cells.

    abstract::Natural killer (NK) cells are potent immune effector cells that can respond to infection and cancer, as well as allowing maternal adaptation to pregnancy. In response to malignant transformation or pathogenic invasion, NK cells can secrete cytokine and may be directly cytolytic, as well as exerting effects indirectly ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2010.78

    authors: Pegram HJ,Andrews DM,Smyth MJ,Darcy PK,Kershaw MH

    更新日期:2011-02-01 00:00:00

  • Poloxamer 407-chitosan grafted thermoresponsive hydrogels achieve synchronous and sustained release of antigen and adjuvant from single-shot vaccines.

    abstract::Sustained-release vaccine delivery systems may enhance the immunogenicity of subunit vaccines and reduce the need for multiple vaccinations. The aim of this study was to develop a thermoresponsive hydrogel using poloxamer 407-chitosan (CP) grafted copolymer as a delivery system for single-shot sustained-release vaccin...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1111/imcb.12031

    authors: Bobbala S,Gibson B,Gamble AB,McDowell A,Hook S

    更新日期:2018-07-01 00:00:00

  • Active and zymogen forms of granzyme B are constitutively released from cytotoxic lymphocytes in the absence of target cell engagement.

    abstract::Granzyme B (GrB) plays a well-established intracellular role in cytotoxic lymphocyte (CL)-mediated killing of abnormal cells; however, emerging evidence suggests that it participates in extracellular matrix remodeling and target cell destruction through anoikis. As these processes require the release of GrB from the C...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2008.98

    authors: Prakash MD,Bird CH,Bird PI

    更新日期:2009-03-01 00:00:00

  • Peroxisome proliferator-activated receptors in tumorigenesis: targets of tumour promotion and treatment.

    abstract::The peroxisome proliferator-activated receptors (PPAR) are ligand-activated transcription factors. There are three genes that code for the PPAR isoforms: PPARalpha, PPARbeta and PPARgamma. In the present review, studies characterizing the various PPAR isoforms are discussed. Peroxisome proliferator-activated receptor ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.2000.00921.x

    authors: Roberts-Thomson SJ

    更新日期:2000-08-01 00:00:00

  • Shining LIGHT on the metabolic role of the cytokine TNFSF14 and the implications on hepatic IL-6 production.

    abstract::The cytokine Tumor Necrosis Factor Superfamily member 14, TNFSF14 (or LIGHT), is a controversial player in numerous diseases. We investigated the role of endogenously expressed TNFSF14 in diet-induced obesity in vivo. Firstly, we studied the effects of Tnfsf14 ablation on the development of obesity, glucose intoleranc...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1111/imcb.1002

    authors: Saunders BM,Rudnicka C,Filipovska A,Davies S,Ward N,Hricova J,Schlaich MP,Matthews VB

    更新日期:2018-01-01 00:00:00

  • Immune stimulating complexes as mucosal vaccines.

    abstract::There is a need for non-living adjuvant vectors that will allow a full range of local and systemic immune responses to orally administered purified antigens. Here we describe our experience with lipophilic immune-stimulating complexes (ISCOMs) containing the saponin adjuvant Quil A. When given orally, ISCOMs containin...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.1998.00749.x

    authors: Smith RE,Donachie AM,Mowat AM

    更新日期:1998-06-01 00:00:00

  • Tumour necrosis factor and lymphotoxin: molecular aspects and role in tissue-specific autoimmunity.

    abstract::Tumour necrosis factor (TNF) is a highly potent, proinflammatory cytokine with broad-ranging functions from the regulation of endothelial cell adhesion molecules to facilitate entry of leucocytes into tissues, to direct induction of cellular cytotoxicity. This diversity of function potentially attributable to TNF in t...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.1996.77

    authors: Körner H,Sedgwick JD

    更新日期:1996-10-01 00:00:00

  • Regulation of caspase activation and apoptosis by cellular zinc fluxes and zinc deprivation: A review.

    abstract::Non-toxic agents that target intracellular signalling pathways in apoptosis may have potential therapeutic use in many diseases. One such agent is the transition metal Zn, a dietary cytoprotectant and anti-oxidant, which stimulates cell proliferation and suppresses apoptosis. Zn is maintained in discrete subcellular p...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.1999.00825.x

    authors: Chai F,Truong-Tran AQ,Ho LH,Zalewski PD

    更新日期:1999-06-01 00:00:00

  • Dengue virus NS1 protein activates immune cells via TLR4 but not TLR2 or TLR6.

    abstract::The secreted hexameric form of the dengue virus (DENV) non-structural protein 1 (NS1) has recently been shown to elicit inflammatory cytokine release and disrupt endothelial cell monolayer integrity. This suggests that circulating NS1 contributes to the vascular leak that plays a major role in the pathology of dengue ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2017.5

    authors: Modhiran N,Watterson D,Blumenthal A,Baxter AG,Young PR,Stacey KJ

    更新日期:2017-05-01 00:00:00

  • Long noncoding RNAs: a potent source of regulation in immunity and disease.

    abstract::The discovery of functional long noncoding RNAs (lncRNAs) coupled with the ever-increasing accessibility of genomic and transcriptomic technology has led to an explosion of functional and mechanistic investigation and discovery into what was once dismissed as junk DNA. Over the past decade, a significant number of lnc...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2015.2

    authors: Yu AD,Wang Z,Morris KV

    更新日期:2015-03-01 00:00:00

  • Anti-ovine VCAM-1 monoclonal antibodies inhibit adhesion and proliferation between sheep endothelial and mononuclear cells in vitro.

    abstract::This paper reports the production and characterization of three monoclonal antibodies (mAb) recognizing ovine vascular cell adhesion molecule-1 (VCAM-1). The mAb were raised against sheep umbilical vein endothelial cells (ShUVEC) and flow cytometric analysis demonstrated that one mAb, QE4G9, was cross-reactive with hu...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1997.85

    authors: Grooby WL,Krishnan R,Russ GR

    更新日期:1997-12-01 00:00:00

  • Human cytomegalovirus UL7, a homologue of the SLAM-family receptor CD229, impairs cytokine production.

    abstract::Human cytomegalovirus (HCMV), the β-herpesvirus prototype, has evolved a wide spectrum of mechanisms to counteract host immunity. Among them, HCMV uses cellular captured genes encoding molecules capable of interfering with the original host function or of fulfilling new immunomodulatory tasks. Here, we report on UL7, ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2011.55

    authors: Engel P,Pérez-Carmona N,Albà MM,Robertson K,Ghazal P,Angulo A

    更新日期:2011-10-01 00:00:00

  • The corneal response to infection with Staphylococcus aureus in the absence of interleukin-4.

    abstract::Interleukin-4 (IL-4) has previously been implicated in a protective response to Staphylococcus aureus corneal infection. Consequently, the specific role of IL-4 during S. aureus corneal infection was investigated using IL-4 gene knockout mice. The eyes of IL-4-/- mice and wild-type mice were challenged topically with ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/sj.icb.7100043

    authors: Cole N,Hume EB,Khan S,Garthwaite L,Schubert T,Reeve V,Willcox MD

    更新日期:2007-06-01 00:00:00

  • An hypothesis to explain why cell-mediated immunity alone can contain infections by certain intracellular parasites and how immune class regulation of the response against such parasites can be subverted.

    abstract::Cells with a low density of parasite-specific antigens on their surface are postulated to be susceptible to a cell-mediated attack but not to effector mechanisms normally activated following the binding of specific antibody to the infected cell. It is further postulated that such infected cells normally induce a cell-...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.1992.44

    authors: Bretscher PA

    更新日期:1992-10-01 00:00:00

  • Fluorescent dyes for lymphocyte migration and proliferation studies.

    abstract::Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as bein...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.1999.00877.x

    authors: Parish CR

    更新日期:1999-12-01 00:00:00

  • Mycobacterium tuberculosis impairs dendritic cell response by altering CD1b, DC-SIGN and MR profile.

    abstract::During a chronic infection such as tuberculosis, the pool of tissue dendritic cells (DC) must be renewed by recruitment of both circulating DC progenitors and monocytes (Mo). However, the microenvironment of the inflammatory site affects Mo differentiation. As DC are critical for initiating a Mycobacterium tuberculosi...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2010.22

    authors: Balboa L,Romero MM,Yokobori N,Schierloh P,Geffner L,Basile JI,Musella RM,Abbate E,de la Barrera S,Sasiain MC,Alemán M

    更新日期:2010-10-01 00:00:00

  • Genetic susceptibility to mycobacterial disease in humans.

    abstract::Mycobacterial disease remains a serious global health problem. Tuberculosis causes more than 2 million deaths a year, and leprosy is still a cause of severe disability in many parts of the world. As a result of the study of individuals with marked susceptibility to usually nonpathogenic mycobacteria, as well as case-c...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1711.2006.01420.x

    authors: Fernando SL,Britton WJ

    更新日期:2006-04-01 00:00:00

  • How the thymus shaped immunology and beyond.

    abstract::Barely 60 years ago, the thymus was considered to be a vestigial organ with no known function or just a graveyard for dying lymphocytes. Now, the thymus and its cells cover a vast area of immunology, genetics and epigenetics relating to medicine, including inflammation, infection, vaccination, dysbiosis, immunodeficie...

    journal_title:Immunology and cell biology

    pub_type: 传,历史文章,信件

    doi:10.1111/imcb.12220

    authors: Miller J

    更新日期:2019-03-01 00:00:00

  • Mucosal adjuvants and delivery systems for protein-, DNA- and RNA-based vaccines.

    abstract::Almost all vaccinations today are delivered through parenteral routes. Mucosal vaccination offers several benefits over parenteral routes of vaccination, including ease of administration, the possibility of self-administration, elimination of the chance of injection with infected needles, and induction of mucosal as w...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1711.2004.01288.x

    authors: Vajdy M,Srivastava I,Polo J,Donnelly J,O'Hagan D,Singh M

    更新日期:2004-12-01 00:00:00

  • Antigen-specific apoptosis in immortalized T cells by soluble MHC class II-peptide complexes.

    abstract::The recognition of T cell receptors (TCR) by purified major histocompatibility complex (MHC) class II-peptide complexes in the absence of costimulatory signals leads to the induction of T cell non-responsiveness or anergy. In a recent study using human T cell clones, it was observed that prolonged incubation of restin...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1996.13

    authors: Arimilli S,Mumm JB,Nag B

    更新日期:1996-02-01 00:00:00

  • Ikaros, Aiolos and Helios: transcription regulators and lymphoid malignancies.

    abstract::Ikaros, Aiolos and Helios encode zinc finger transcription factors that are important regulators of lymphoid development and differentiation. These proteins are involved in the control of gene expression and when associated with nuclear complexes, participate in nucleosome remodeling. Because differential splicing pro...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.2003.01159.x

    authors: Rebollo A,Schmitt C

    更新日期:2003-06-01 00:00:00

  • Protection against non-typable Haemophilus influenzae following sensitization of gut associated lymphoid tissue: role of specific antibody and phagocytes.

    abstract::Rats intestinally immunized with whole killed non-typable Haemophilus influenzae clear this organism from the lungs faster than non-immunized rats. This study investigated the role of antibody and phagocytes in the clearance mechanism. First, dose-response experiments demonstrated that while lowering the dose of non-t...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1995.42

    authors: Wallace FJ,Witt CS,Clancy RL,Cripps AW

    更新日期:1995-06-01 00:00:00

  • Production of interleukin-1 alpha and interleukin-2 by separate, phenotypically different leukaemia and human T cell lymphotropic virus-1-transformed T cell clones.

    abstract::The relationship between interleukin-1 (IL-1) and interleukin-2 (IL-2) production and immunophenotype marker profiles was studied in a panel of 29 leukaemia and human T cell lymphotropic virus-1 (HTLV-1)-transformed T cell lines. Culture supernatants from six of the 29 T cell lines tested increased IL-2 production by ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1993.56

    authors: Holán V,Minowada J

    更新日期:1993-12-01 00:00:00

  • An antigen valence theory to explain the evolution and organization of the humoral immune response.

    abstract::The three modes of antibody production, natural, T independent (TI) and T dependent (TD) are conserved among vertebrate species suggesting an important role for each in protection against pathogens. Here, I use an artificial 'universe' to argue that the three modes of antibody production represent layers that evolved ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1997.95

    authors: Hodgkin PD

    更新日期:1997-12-01 00:00:00

  • Redox balance of mouse medullary CD4 single-positive thymocytes.

    abstract::After positive selection, the newly differentiated single-positive (SP) thymocytes undergo negative selection to eliminate autoreactive T cells, functional maturation to acquire immunocompetence and egress capability. To investigate whether the intracellular reduction/oxidation (redox) balance has an important role on...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2013.57

    authors: Jin R,Teng F,Xu X,Yao Y,Zhang S,Sun X,Zhang Y,Ge Q

    更新日期:2013-11-01 00:00:00

  • Diesel exhaust particulate matter induces multinucleate cells and zinc transporter-dependent apoptosis in human airway cells.

    abstract::The cellular effects of biodiesel emissions particulate matter (BDEP) and petroleum diesel emissions particulate matter (PDEP) were compared using a human airway cell line, A549. At concentrations of 25 microg/ml, diesel particulate matter induced the formation of multinucleate cells. In cells treated with a mixture o...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/sj.icb.7100109

    authors: Ackland ML,Zou L,Freestone D,van de Waasenburg S,Michalczyk AA

    更新日期:2007-11-01 00:00:00

  • Functional aspects of iscoms.

    abstract::The iscom is a delivery system, designed for both parenteral and mucosal modes of administration, for both antigens and adjuvants, components which are interchangeable. By the parenteral route a prominent systemic Th1 type of response is evoked, but the mucosal immunoglobulin A (IgA) response was insignificant. Intran...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.1998.00756.x

    authors: Morein F,Bengtsson KL

    更新日期:1998-08-01 00:00:00

  • The ecology and pathology of Epstein-Barr virus.

    abstract::Epstein-Barr virus achieves its ubiquitous and uniform epidemiological distribution by a dual strategy of latency to guarantee lifelong persistence and intermittent replication to guarantee transmission. These two functions appear to dictate residence in different cell types: latency in B lymphocytes and replication i...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.1995.79

    authors: Schmidt CW,Misko IS

    更新日期:1995-12-01 00:00:00

  • Antigenic analysis of Strongyloides ratti infective larvae and adult worms.

    abstract::The protein composition of Strongyloides ratti infective larvae and adult worms extracted sequentially in water, sodium deoxycholate and sodium dodecyl sulphate (SDS) and their excretory/secretory products were analysed by both one- and two-dimensional SDS-polyacrylamide gel electrophoresis. While many bands common to...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1987.26

    authors: Northern C,Grove DI

    更新日期:1987-06-01 00:00:00