Abstract:
:Antinuclear antibodies (ANAs) are autoantibodies that attack self-proteins within cell nucleus structures; their presence in serum may indicate an autoimmune disease. Also, positive ANA test results have been obtained in chronic infectious diseases, cancers, medication-related adverse events, and even healthy individuals. As a result, a correct interpretation of the presence of ANAs is needed.Identification of ANAs subtypes is an important part of clinical immunology. The presence of ANAs in patient blood specimens is detected using a cell-line substrate from human laryngeal carcinoma (HEp-2 cells). On this substrate, ANAs will bind specific antigens, which will lead to a suggestive fluorescent emission. The fluorescence patterns visualized under the fluorescence microscope can be correlated with certain subtypes of ANA and certain autoimmune diseases.Depending on the subtype of ANA present in the serum and the targeted antigen, several staining patterns are reported, namely, nuclear patterns, nucleolar patterns, cell cycle patterns, or cytoplasmatic patterns. Identification of a certain pattern can lead to diagnosis of a certain autoimmune disease.
journal_name
Lab Medjournal_title
Laboratory medicineauthors
Sur LM,Floca E,Sur DG,Colceriu MC,Samasca G,Sur Gdoi
10.1093/labmed/lmy024subject
Has Abstractpub_date
2018-07-05 00:00:00pages
e62-e73issue
3eissn
0007-5027issn
1943-7730pii
5032495journal_volume
49pub_type
杂志文章,评审abstract::Hereditary hemochromatosis (HH) is an autosomal recessive disorder of iron metabolism characterized by increased iron absorption and tissue deposition. Three loss-of-function mutations in the hemochromatosis gene (HFE), namely, C282Y (c.845G>A), H63D (c.187C>G), and S65C (c.193A>T), account for the vast majority of HH...
journal_title:Laboratory medicine
pub_type: 杂志文章,评审
doi:10.1093/labmed/lmy065
更新日期:2019-04-08 00:00:00
abstract:OBJECTIVE:To identify the osteoprotegerin (OPG) correlates with antiphospholipid syndrome (APS) parameters. METHODS:Our cohort included 40 patients with primary APS disease associated with systemic lupus erythematosus (SLE) (mean age, 43.7 years; 87% female). Data on cardiovascular risk factors and specific clinical e...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz051
更新日期:2020-03-10 00:00:00
abstract::Hormographiella is a rare fungal pathogen in humans; however, case reports have described disseminated infection in immunocompromised hosts. This pathogen has been described to yield poor prognosis in patients who harbor it. Herein, we present a case report of autopsy-proven disseminated Hormographiella aspergillata i...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz018
更新日期:2019-10-10 00:00:00
abstract:BACKGROUND:Research in several professional fields has demonstrated that delays (time lapse) in taking certification examinations may result in poorer performance by examinees. Thirteen states and/or territories require licensure for laboratory personnel. A core component of licensure is passing a certification exam. A...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMNM534LIACPBZWH
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. METHODS:Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacua...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lm2xcv5sqml1ontm
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:To determine the effect of a modified request form on the total use of γ-glutamyl transferase (GGT) usage and the ratio of inappropriate testing. METHODS:We modified a test request form by moving GGT from the "Liver profile" to the "Other" section. The criteria for appropriate GGT ordering were developed ba...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LM7E5LG6PWJYEFUJ
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:To evaluate broad-range 16S ribosomal DNA (rDNA) polymerase chain reaction (PCR) as a rapid screening tool to detect bacterial contamination of stem-cell products. METHODS:We performed the evaluation using whole blood spiked with serially diluted bacterial-type strains. Detection sensitivity was defined as t...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMKT4P9FFI2BBSIU
更新日期:2015-01-01 00:00:00
abstract::Rhizobium radiobacter is an opportunistic, usually saprophytic, gram-negative bacillus found in agricultural soil. Isolation from blood has been reported most often in hospitalized patients harboring malignant neoplasms or human immunodeficiency virus (HIV) associated immunosuppression, who have catheter or medical de...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw019
更新日期:2016-08-01 00:00:00
abstract:PATIENT:66-year-old ethnic Egyptian man. CHIEF COMPLAINT:Hematuria. HISTORY OF PRESENT ILLNESS:The patient had a history of multiple episodes of gross hematuria for the past 5 years. Because the hematuria usually resolved on its own, he did not seek medical attention during that time. Bladder cancer was suspected. P...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LM96EJPPBYADIOVC
更新日期:2015-10-01 00:00:00
abstract::The COVID-19 pandemic has led to a major setback in both the health and economic sectors across the globe. The scale of the problem is enormous because we still do not have any specific anti-SARS-CoV-2 antiviral agent or vaccine. The human immune system has never been exposed to this novel virus, so the viral interact...
journal_title:Laboratory medicine
pub_type: 杂志文章,评审
doi:10.1093/labmed/lmaa049
更新日期:2021-01-04 00:00:00
abstract:BACKGROUND:Encephalitis is caused by infection, immune mediated diseases, or primary inflammatory diseases. Of all the causative infectious pathogens, 90% are viruses or bacteria. Granulomatous amoebic encephalitis (GAE), caused by Balamuthia mandrillaris, is a rare but life-threatening disease. Diagnosis and therapy a...
journal_title:Laboratory medicine
pub_type: 杂志文章,评审
doi:10.1093/labmed/lmz079
更新日期:2020-03-10 00:00:00
abstract:BACKGROUND:Preparation of the patient for laboratory tests is crucial. Our aim was to investigate the current practice and regional variability of recommendations regarding patient preparation for laboratory testing. METHODS:A call for data was posted by email. Spanish laboratories were invited to fill out and submit ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz092
更新日期:2020-05-06 00:00:00
abstract:OBJECTIVE:To evaluate and compare the performances of the automated urinalysis devices UX-2000 and Cobas 6500. METHOD:A total of 258 urine specimens were collected from the routine specimen workload. We analyzed all specimens on both automated instruments and recorded the turnaround time from each method. Physical, ch...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw002
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:Platelet counts generated by automated analyzers on blood specimens that contain platelet clumps are often inaccurate and require verification by blood-smear review. In this study, we assessed the reliability of the Sysmex XE-5000 instrument to detect platelet clumps. METHOD:We reviewed automated complete bl...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw016
更新日期:2016-08-01 00:00:00
abstract::Caspases play a vital role during apoptosis. In addition to apoptosis, caspases play a role in cytokine gene induction and work to inhibit apoptosis. In order for individuals to thrive with useful tissue growth, the rate of cell growth and division must surpass the rate of cell division. It is well established that ex...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmx086
更新日期:2018-03-21 00:00:00
abstract::In the past few years, interest has increased in cell-derived microparticles (MPs), which are defined by their size of from 0.1 to 1 μm, and can be derived from various cell types, including endothelial cells, leukocytes, red blood cells (RBCs), and platelets. These MPs carry negatively charged phosphatidylserine (PS)...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmaa043
更新日期:2020-08-20 00:00:00
abstract::We report a case of a false-positive HIV result in an apparently healthy pregnant woman. Since no prenatal HIV testing had been performed, we screened for HIV reactivity utilizing the Architect HIV-Ag/Ab Combo assay. Results obtained were inconsistent in that they were repeatedly HIV reactive on a single serum sample ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMAAGVXK05LUWOQN
更新日期:2014-07-01 00:00:00
abstract::Health systems face intense pressure to decrease costs and improve services as the health care delivery system in the United States undergoes tremendous change due to health care reform. As health systems grow, like any business, they are forced to explore standardization to realize and maintain efficient practices. C...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw069
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND:To compare the coagulation-factor profile of cryoprecipitate produced from fresh frozen plasma from whole blood (WB) stored for 24 hours at room temperature (24CP) with that of standard cryoprecipitate (CP). METHODS:We collected 80 units of WB from healthy volunteers, of which 20 units were of each blood gr...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lmv1e84uctrqqzp
更新日期:2014-04-01 00:00:00
abstract::Tacrolimus (Tac) is an immunosuppressive drug that is used in preventing organ and tissue rejection in patients after transplantation. Tac administration requires frequent and diligent monitoring by physicians to ensure proper dosage and to limit the potential for harmful adverse effects, which can include renal damag...
journal_title:Laboratory medicine
pub_type: 杂志文章,评审
doi:10.1093/labmed/lmx056
更新日期:2017-11-08 00:00:00
abstract::Waldenström macroglobulinemia (WM) is a form of lymphoplasmacytic lymphoma that can cause hyperviscosity syndrome due to unchecked monoclonal antibody production. Some patients are also found to have associated cryoglobulinemia, which can cause systemic complications including vasculitis, renal disease, and pulmonary ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmy073
更新日期:2019-07-16 00:00:00
abstract::Cell-derived microparticles (MPs) are small fragments released from various cells when they are activated or undergo apoptosis. In the field of transfusion medicine, a number of studies have documented increased levels of MPs in blood products, which have been associated with multiple factors, including donor variabil...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz100
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:To establish a new indicator derived from reticulocyte hemoglobin (Ret-He) content and red blood cell (RBC) indices for screening for iron deficiency anemia (IDA) in an area in whch thalassemia is prevalent. METHODS:Blood specimens from 304 women aged between 18 and 30 years residing in northeast Thailand we...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz099
更新日期:2020-09-01 00:00:00
abstract:Objective:To compare a manual squamous cell carcinoma antigen (SCCA) assay and an automated SCCA assay in a clinical setting. Methods:We included, in this study, a total of 158 specimens that had been tested using the manual SCCA assay. The CanAg SCC EIA assay and the Elecsys SCC were compared for their clinical setti...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmy003
更新日期:2018-07-05 00:00:00
abstract:OBJECTIVE:To test a hybrid polyethylene glycol (PEG)/gel method, specifically to attempt to increase reaction strength of accidentally overdiluted anti-Cob and broadly for proof of concept. METHODS:Methods were divided into 2 basic steps: sensitization and antiglobulin (AHG) testing. Sensitization was performed with P...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz015
更新日期:2020-03-10 00:00:00
abstract:Background:Anti-phospholipase A2 receptor (anti-PLA2R) is a promising biomarker for diagnosis, activity evaluation, therapy monitoring, and prognostic estimation of primary membranous neuropathy (pMN). Difference in methodology may be one cause of the discrepancy in anti-PLA2R-positive percentages in reported studies. ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmy016
更新日期:2018-10-11 00:00:00
abstract:Background:Interest in vitamin D has increased during the past 2 decades, with a corresponding increase in laboratory testing of 25-hydroxyvitamin D [25(OH)D]. The vast majority of specimens tested display normal or deficient levels of 25(OH)D; concentrations rarely fall in the potentially toxic range. Methods:We perf...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmx077
更新日期:2018-03-21 00:00:00
abstract:OBJECTIVE:To determine whether K2-ethylenediaminetetraacetic acid (EDTA) and K3-EDTA Greiner tubes could be used interchangeably for glycosylated hemoglobin, type A1C (HbA1c) measurement via the Abbott Laboratories ARCHITECT chemiluminescent microparticle HbA1c assay on the ARCHITECT i2000SR immunoanalyzer at our unive...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmv016
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:Because of the rapidly emerging SARS-CoV-2 pandemic and its wide public health challenges, rapid diagnosis is essential to decrease the spread. Antigen-based rapid detection tests are available; however, insufficient data about their performance are available. METHODS:The lateral-flow immunochromatographic B...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmaa104
更新日期:2020-12-07 00:00:00
abstract:OBJECTIVE:Auramine-phenol stain was compared with Kinyoun's acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost. METHODS:...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lms0wn7k7ukzcpmi
更新日期:2014-01-01 00:00:00