Detection of a Borrelia species in questing Gulf Coast ticks, Amblyomma maculatum.

Abstract:

:Borrelia spp. are agents of Lyme disease and relapsing fever, diseases which use Ixodes hard ticks and Ornithodoros soft ticks, respectively, as primary vectors. Some relapsing fever spirochetes, such as B. miyamotoi, are also found in hard ticks. To date, no Borrelia sp. is known to use the hard tick, Amblyomma maculatum, as a vector. However, both B. burgdorferi and B. lonestari were recently detected in A. maculatum removed from hosts. In our study, DNA extracts from 306 questing adult A. maculatum collected in Mississippi in 2009 and 2010 were tested for Borrelia spp. DNA by PCR amplification of flaB and 16S rRNA gene targets. An additional 97 A. maculatum collected in 2013 were tested by amplification of 16S rRNA gene target. Two ticks, one collected in 2009 and the other in 2010, were positive by PCR of the flaB and 16S rRNA gene targets; both were collected from the same location in central Mississippi. Interestingly, 16S rRNA gene amplicons from these two tick extracts were 98% identical to twelve Borrelia spp. including the reptile-associated spirochete B. turcica and Borrelia sp. "tAG158M"; flaB amplicons from these two ticks shared closest identity (89%) to the reptile-associated spirochete, B. turcica. These results demonstrate a Borrelia sp. in unfed A. maculatum ticks that is unique from other species in the NCBI database and in a clade with reptile-associated Borrelia species. Detection of a previously unrecognized Borrelia in a hard tick species generates additional questions regarding the bacterial fauna in these arthropods and warrants further studies to better understand this fauna.

journal_name

Ticks Tick Borne Dis

authors

Lee JK,Smith WC,McIntosh C,Ferrari FG,Moore-Henderson B,Varela-Stokes A

doi

10.1016/j.ttbdis.2014.02.004

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

449-52

issue

4

eissn

1877-959X

issn

1877-9603

pii

S1877-959X(14)00059-4

journal_volume

5

pub_type

杂志文章
  • Duration of Borrelia mayonii infectivity in an experimental mouse model for feeding Ixodes scapularis larvae.

    abstract::A novel species within the Borrelia burgdorferi sensu lato complex, Borrelia mayonii, was recently described and found to be associated with Lyme borreliosis in the Upper Midwest of the United States. The blacklegged tick, Ixodes scapularis, is naturally infected with B. mayonii in the Upper Midwest and has been exper...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2016.11.002

    authors: Dolan MC,Breuner NE,Hojgaard A,Hoxmeier JC,Pilgard MA,Replogle AJ,Eisen L

    更新日期:2017-01-01 00:00:00

  • Trends in surveillance data of human Lyme borreliosis from six federal states in eastern Germany, 2009-2012.

    abstract::Lyme borreliosis (LB) is the most frequent vector-borne disease in Germany. For more than 10 years, data from mandatory notifications have been available from 6 federal states in the eastern part of Germany. A common case definition was applied. Clinical manifestations of erythema migrans, neuroborreliosis (radiculone...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2013.10.010

    authors: Wilking H,Stark K

    更新日期:2014-04-01 00:00:00

  • Capybaras (Hydrochoerus hydrochaeris) as amplifying hosts of Rickettsia rickettsii to Amblyomma sculptum ticks: Evaluation during primary and subsequent exposures to R. rickettsii infection.

    abstract::Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), the most lethal tick-borne disease in the western hemisphere. Amblyomma sculptum is the main vector of R. rickettsii in southeastern Brazil. Capybaras act as primary hosts for this tick species, and as amplifying hosts for R. rickettsii, creating new...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101463

    authors: Ramírez-Hernández A,Uchoa F,Serpa MCA,Binder LC,Souza CE,Labruna MB

    更新日期:2020-09-01 00:00:00

  • Occupational risk for personnel working in veterinary clinics through exposure to vectors of rickettsial pathogens.

    abstract::Workers at veterinary clinics are exposed to zoonotic pathogens, which, though infrequently, can lead to serious consequences. The aim of the present study was to estimate the seroprevalence for rickettsial pathogens in veterinary clinic personnel in Ciudad Juárez, Chihuahua, Mexico. Voluntary participants included 10...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.10.012

    authors: Escárcega-Ávila AM,de la Mora-Covarrubias A,Quezada-Casasola A,Jiménez-Vega F

    更新日期:2019-02-01 00:00:00

  • Response to letter regarding article "How far north are migrant birds transporting the tick Ixodes scapularis in Canada? Insights from stable hydrogen isotope analyses of feathers".

    abstract::In this letter we respond to correspondence and clarify the intent and importance of our article. ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 评论,信件

    doi:10.1016/j.ttbdis.2015.12.002

    authors: Ogden NH,Barker IK,Francis CM,Heagy A,Lindsay LR,Hobson KA

    更新日期:2016-03-01 00:00:00

  • Prevalence of Ehrlichia chaffeensis and Ehrlichia ewingii in Amblyomma americanum and Dermacentor variabilis collected from southeastern Virginia, 2010-2011.

    abstract::Amblyomma americanum is the most commonly encountered tick species in southeastern Virginia, representing approximately 95% of the human-biting tick population in this area. Here we investigated the prevalence of Ehrlichia chaffeensis and Ehrlichia ewingii in questing Amblyomma americanum and Dermacentor variabilis ti...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2014.07.023

    authors: Wright CL,Gaff HD,Hynes WL

    更新日期:2014-10-01 00:00:00

  • A field survey for the seroprevalence of Theileria equi and Babesia caballi in donkeys from Nuu Division, Kenya.

    abstract::Equine piroplasmosis is one of the most significant tick-borne disease of equids. The prevalence of this disease in donkeys of semi-arid Kenya remains largely unexplored. The primary objective of this study was to demonstrate the extent to which donkeys in Nuu division, Kenya have been exposed to the haemoprotozoans B...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.05.015

    authors: Oduori DO,Onyango SC,Kimari JN,MacLeod ET

    更新日期:2015-07-01 00:00:00

  • A surrogate life cycle of Amblyomma ovale Koch, 1844.

    abstract::As ticks spend most of their time off-host, the environment is a major selective force of these parasites. In fact, human impact on landscapes has favored a minority of tick species which became well-known pests. However, this is an ongoing process and novel pests may arise. We herein report a surrogate life cycle of ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2012.06.002

    authors: Szabó MP,Martins TF,Nieri-Bastos FA,Spolidorio MG,Labruna MB

    更新日期:2012-09-01 00:00:00

  • Babesia bovis infection in cattle in the southwestern Brazilian Amazon.

    abstract::The present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bo...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2012.08.001

    authors: Brito LG,Rocha RB,Barbieri Fda S,Ribeiro ES,Vendrami FB,Souza GC,Giglioti R,Regitano LC,Falcoski TO,Tizioto PC,Oliveira MC

    更新日期:2013-02-01 00:00:00

  • Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR).

    abstract::This study investigated the first outbreak of oriental theileriosis in a herd of beef cattle in South Australia using a newly established multiplexed tandem PCR (MT-PCR) to identify, differentiate and quantitate the four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis recognised to occur in Aust...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.04.012

    authors: Gebrekidan H,Gasser RB,Perera PK,McGrath S,McGrath S,Stevenson MA,Jabbar A

    更新日期:2015-07-01 00:00:00

  • Babesia microti thioredoxin 3 is an effective antioxidant and involved in the response to antiprotozoal drugs.

    abstract::The intra-erythrocytic apicomplexan Babesia microti is the predominant pathogen that causes human babesiosis, an infectious disease that occurs worldwide. B. microti relies on the antioxidant including thioredoxin system to maintain the redox balance during the erythrocytic stage. In the present study, the full-length...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.01.021

    authors: Huang J,Xiong K,Zhang H,Zhao Y,Cao J,Zhou Y,Gong H,Zhou J

    更新日期:2018-03-01 00:00:00

  • Coxiella symbiont in the tick Ornithodoros rostratus (Acari: Argasidae).

    abstract::In the present study, the presence of tick-associated bacteria and protozoa in Ornithodoros rostratus ticks (adults, nymphs, and eggs) from the Pantanal region of Brazil were determined by molecular detection. In these ticks, DNA from protozoa in the genera Babesia and Hepatozoon, and bacteria from the genera Ricketts...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2012.02.003

    authors: Almeida AP,Marcili A,Leite RC,Nieri-Bastos FA,Domingues LN,Martins JR,Labruna MB

    更新日期:2012-09-01 00:00:00

  • Molecular detection of tick-borne pathogens in Ixodes ricinus from Moldova collected in 1960.

    abstract::This study is the first report about the prevalence of tick-borne pathogens, as well as their (co-)infection rates, in the museum-archived I. ricinus female ticks collected in Moldova in 1960. A total of 16.7% (21/126) ticks was mono-infected. Borrelia burgdorferi sensu stricto was revealed as the most abundant specie...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2012.12.004

    authors: Movila A,Toderas I,Uspenskaia I,Conovalov J

    更新日期:2013-06-01 00:00:00

  • Demonstration of lumpy skin disease virus infection in Amblyomma hebraeum and Rhipicephalus appendiculatus ticks using immunohistochemistry.

    abstract::Lumpy skin disease (LSD) is caused by lumpy skin disease virus (LSDV), a member of the genus Capripoxvirus. Transmission of the virus has been associated with haematophagous insects such as Stomoxys calcitrans as well as Aedes and Culex species of mosquitoes. Recent studies have reported the transmission of the virus ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2013.09.010

    authors: Lubinga JC,Clift SJ,Tuppurainen ES,Stoltsz WH,Babiuk S,Coetzer JA,Venter EH

    更新日期:2014-03-01 00:00:00

  • Molecular identification and evaluation of Coxiella-like endosymbionts genetic diversity carried by cattle ticks in Algeria.

    abstract::Coxiella-like bacteria are a large group of yet-to-isolate and characterize bacteria phylogenetically close to the agent of Q fever, Coxiella burnetii, and often associated with ixodid ticks worldwide. This study was designed to assess the presence of Coxiella-like endosymbionts (CLE) in ticks and to describe their ge...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101493

    authors: Rahal M,Medkour H,Diarra AZ,Bitam I,Parola P,Mediannikov O

    更新日期:2020-09-01 00:00:00

  • Molecular detection and characterization of Hepatozoon spp. in dogs from the central part of Turkey.

    abstract::Canine hepatozoonosis is a tick-borne protozoal disease caused by Hepatozoon spp. Two species of Hepatozoon are currently known to infect dogs as Hepatozoon canis and H. americanum. Although H. canis generally causes a chronic infection with relatively mild clinical alterations compared to H. americanum, infection by ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.03.004

    authors: Aydin MF,Sevinc F,Sevinc M

    更新日期:2015-04-01 00:00:00

  • Occurrence of Ixodiphagus hookeri (Hymenoptera: Encyrtidae) in Ixodes ricinus (Acari: Ixodidae) in southern Italy.

    abstract::Natural enemies of ticks include the parasitoid wasp Ixodiphagus hookeri (Hymenoptera: Encyrtidae). The aim of this preliminary study was to investigate the occurrence of I. hookeri DNA in a community of ticks (Ixodes ricinus, Dermacentor marginatus, Hyalomma marginatum, Haemaphysalis inermis and Rhipicephalus turanic...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.01.001

    authors: Ramos RA,Campbell BE,Whittle A,Lia RP,Montarsi F,Parisi A,Dantas-Torres F,Wall R,Otranto D

    更新日期:2015-04-01 00:00:00

  • Biological control of Ixodes ricinus larvae and nymphs with Metarhizium anisopliae blastospores.

    abstract::The entomopathogenic fungus Metarhizium anisopliae is used as a biological pest control agent against various arthropod species, including ticks. However, the efficacy depends on tick species, tick stage and fungus strain. We studied the effect of M. anisopliae on engorged larvae and nymphs of Ixodes ricinus, the most...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2016.03.010

    authors: Wassermann M,Selzer P,Steidle JLM,Mackenstedt U

    更新日期:2016-07-01 00:00:00

  • Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic).

    abstract::The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2017.04.001

    authors: Kartashov MY,Glushkova LI,Mikryukova TP,Korabelnikov IV,Egorova YI,Tupota NL,Protopopova EV,Konovalova SN,Ternovoi VA,Loktev VB

    更新日期:2017-06-01 00:00:00

  • Genotyping amitraz resistance profiles in Rhipicephalus microplus Canestrini (Acari: Ixodidae) ticks from Punjab, India.

    abstract::Acaricide resistance is one of the greatest threats to sustainable and effective control of vector ticks worldwide. The amitraz resistance status in cattle tick, Rhipicephalus microplus populations collected from 18 districts of Punjab in north-western India were characterized using bioassay and molecular assays. The ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101578

    authors: Jyoti,Singh NK,Singh H,Singh NK,Rath SS

    更新日期:2021-01-01 00:00:00

  • Genetic structure analysis of Amblyomma mixtum populations in Veracruz State, Mexico.

    abstract::Amblyomma mixtum Koch, 1844 parasitizes livestock, humans, and wildlife in Mexico. However, information on population genetics for this tick species in the country is missing. Tick samples were collected from livestock in ten regions across the state of Veracruz (22°28'N, 17°09'S, 93°36'E, 98°39'W) to analyze the gene...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2018.09.004

    authors: Aguilar-Domínguez M,Sánchez-Montes S,Esteve-Gassent MD,Barrientos-Salcedo C,Pérez de León A,Romero-Salas D

    更新日期:2019-01-01 00:00:00

  • First molecular detection and characterization of tick-borne pathogens in water buffaloes in Bohol, Philippines.

    abstract::The water buffalo industry is a vital part of the Philippine livestock economy and is an essential contributor to the developing local dairy industry. Although relatively less susceptible to diseases, water buffaloes can still be infected and can act as reservoirs of tick-borne pathogens (TBPs). However, limited infor...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2019.03.016

    authors: Galon EMS,Adjou Moumouni PF,Ybañez RHD,Ringo AE,Efstratiou A,Lee SH,Liu M,Guo H,Gao Y,Li J,Salces CB,Maurillo BCA,Boldbaatar D,Ybañez AP,Xuan X

    更新日期:2019-06-01 00:00:00

  • Tick-borne disease preventive practices and perceptions in an endemic area.

    abstract::Lyme disease is the most commonly reported vector-borne illness in the United States. Since the institution of Nationally Notifiable surveillance efforts for Lyme disease in the United States in 1991, there has been a consistent increase in the number of reported cases. Thus, the need for targeted prevention strategie...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.12.003

    authors: Butler AD,Sedghi T,Petrini JR,Ahmadi R

    更新日期:2016-03-01 00:00:00

  • Seasonal activity patterns of the western black-legged tick, Ixodes pacificus, in relation to onset of human Lyme disease in northwestern California.

    abstract::Seasonal activity patterns of questing western black-legged ticks, Ixodes pacificus were investigated in northwestern California. Adult I. pacificus became active in the fall (late October/early November) and their appearance was associated with the first rain of the season. Following a peak in January, the abundance ...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2014.05.002

    authors: Salkeld DJ,Castro MB,Bonilla D,Kjemtrup A,Kramer VL,Lane RS,Padgett KA

    更新日期:2014-10-01 00:00:00

  • Borrelia spp. in ticks and birds from a protected urban area in Buenos Aires city, Argentina.

    abstract::This study was aimed to know epidemiological aspects of Borrelia spp. in a protected urban area of Buenos Aires city, Argentina, where thousands of people visit this area for recreational purposes. Ticks were collected from vegetation, birds and dogs. Three hundred and forty birds belonging to 43 species, 41 genera, 1...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2019.101282

    authors: Cicuttin GL,De Salvo MN,Venzal JM,Nava S

    更新日期:2019-10-01 00:00:00

  • The role of Ixodes scapularis, Borrelia burgdorferi and wildlife hosts in Lyme disease prevalence: A quantitative review.

    abstract::Due to the ongoing expansion of Ixodes scapularis (blacklegged tick) throughout the northeastern and midwestern United States, there is need to identify the role wildlife hosts play in the establishment and maintenance of tick populations. To quantify and synthesize the patterns of I. scapularis and Borrelia burgdorfe...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章,meta分析,评审

    doi:10.1016/j.ttbdis.2018.04.006

    authors: Halsey SJ,Allan BF,Miller JR

    更新日期:2018-07-01 00:00:00

  • Identification of tick species and disseminate pathogen using hemolymph by MALDI-TOF MS.

    abstract:BACKGROUND:Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. The challenges of the present study were to assess MALDI-TOF MS profiling for the both ident...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2015.04.013

    authors: Yssouf A,Almeras L,Berenger JM,Laroche M,Raoult D,Parola P

    更新日期:2015-07-01 00:00:00

  • Collection of immature Dermacentor reticulatus (Fabricius, 1794) ticks from vegetation and detection of Rickettsia raoultii in them.

    abstract::It is commonly assumed that Dermacentor reticulatus immature life stages are nidicolous and therefore cannot be collected from vegetation. However, in June and July of 2018 and 2019, a total of 47 questing D. reticulatus larvae and two nymphs were collected by the flagging method in two different sites close to the ci...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101543

    authors: Schmuck HM,Chitimia-Dobler L,Król N,Kacza J,Pfeffer M

    更新日期:2020-11-01 00:00:00

  • Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation.

    abstract::Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potent...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2017.01.001

    authors: Mihaljica D,Marković D,Radulović Ž,Mulenga A,Ćakić S,Sukara R,Samardžić J,Tomanović S

    更新日期:2017-03-01 00:00:00

  • Genetic variability of Anaplasmataceae circulating in small mammals and ticks in an Ixodes persulcatus/Ixodes trianguliceps sympatric area in Russian Siberia.

    abstract::A total of 705 rodents from Myodes, Microtus, and Apodemus genera, 396 adult questing Ixodes persulcatus, and 115 Ixodes larvae and nymphs taken from rodents (and then molted under laboratory conditions to nymphs and adults) were collected in 2013-2018 in Omsk province, Russian Siberia, and examined for the presence o...

    journal_title:Ticks and tick-borne diseases

    pub_type: 杂志文章

    doi:10.1016/j.ttbdis.2020.101499

    authors: Rar V,Yakimenko V,Tikunov A,Makenov M,Epikhina T,Tancev A,Tikunova N

    更新日期:2020-09-01 00:00:00