Abstract:
:Mouse oocytes at metaphase I of meiotic maturation were treated with puromycin, which caused the condensed chromosomes to become decondensed to form an interphase nucleus. The chromosomes returned to a metaphase state 6.3 hr after the oocytes were transferred to puromycin-free medium [H. J. Clarke and Y. Masui (1983) Dev. Biol. 97, 291-301]. In contrast, the chromosomes of the puromycin-treated oocytes remained decondensed within the nucleus if dibutyryl cyclic AMP (dbcAMP) was included in the puromycin-free medium. This implies that dbcAMP inhibited the development of conditions in the oocytes that were required for the transition to metaphase. The chromosomes of puromycin-treated oocytes that were incubated for 7.5 hr in dbcAMP-containing medium returned to metaphase just 1.9 hr after transfer to dbcAMP-free medium. Therefore, the protein synthesis-dependent process that is required for the transition to metaphase could occur in the presence of dbcAMP. Fusion to metaphase II oocytes, or to puromycin-treated oocytes that had returned to metaphase, rapidly induced transition of the nuclei of dbcAMP-inhibited oocytes to metaphase, despite the presence of the inhibitor. These results suggest that the transition of nuclei to metaphase can be induced by a cytoplasmic factor that is present in metaphase oocytes, and that dbcAMP inhibits the development of this factor.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Clarke HJ,Masui Ydoi
10.1016/0012-1606(85)90006-5subject
Has Abstractpub_date
1985-03-01 00:00:00pages
32-7issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(85)90006-5journal_volume
108pub_type
杂志文章abstract::The regulation of early embryonic development in the amphibian Xenopus laevis depends largely upon translational and post-translational regulatory mechanisms to direct the complex cytodifferentiations that take place during early cleavage and blastula formation. The cell cycle dependence of protein synthesis was exami...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90460-k
更新日期:1991-07-01 00:00:00
abstract::In the nematode Caenorhabditis elegans, the BMP-related growth factor DBL-1 regulates body size and male tail morphogenesis via a conserved receptor/Smad signaling pathway. Smads are transcription factors, but rely on transcription cofactors for appropriate regulation of target genes in response to TGF-beta- and BMP-r...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.02.038
更新日期:2007-05-15 00:00:00
abstract::Mutations impairing the GTPase activity of G protein Galpha subunits can result in activated Galpha subunits that affect signal transduction and cellular responses and, in some cases, promote tumor formation. An analogous mutation in the Dictyostelium Galpha4 subunit gene (Q200L substitution) was constructed and found...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9474
更新日期:1999-11-15 00:00:00
abstract::C. elegans ahr-1 is orthologous to the mammalian aryl hydrocarbon receptor, and it functions as a transcription factor to regulate the development of certain neurons. Here, we describe the role of ahr-1 in a specific behavior: the aggregation of C. elegans on lawns of bacterial food. This behavior is modulated by nutr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.07.017
更新日期:2006-10-15 00:00:00
abstract::The ancestral state of animal gastrulation and its bearing for our understanding of bilaterian evolution still is one of the most controversially discussed topics in the field of evolutionary and developmental biology. One hypothesis, the so-called amphistomy scenario, suggests the presence of a slit-like blastopore i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.04.014
更新日期:2017-07-01 00:00:00
abstract::The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1040
更新日期:1995-02-01 00:00:00
abstract::In this study, we present evidence that neurogenic cells inhibit the differentiation of cardiogenic cells. When cells of the entire area pellucida at stage 5 were dissociated and reaggregated, the aggregates differentiated into neural tissues and other structures of any germ layer origin, except for heart tissues, des...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1266
更新日期:1995-09-01 00:00:00
abstract::BMP signaling responses are refined by distinct secreted and intracellular antagonists in different cellular and temporal contexts. Here, we show that the nuclear LEM-domain protein MAN1 is a tissue-specific antagonist of BMP signaling in Drosophila. MAN1 contains two potential Mad-binding sites. We generated MAN1Delt...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.11.036
更新日期:2010-03-01 00:00:00
abstract::We have been studying oligodendrocyte generation in vitro to obtain insights into how the timely generation of these cells might be regulated. Our studies suggest the existence of timing mechanisms quite different from those of existing models, wherein it is proposed that timely oligodendrocyte generation is associate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0280
更新日期:1996-11-25 00:00:00
abstract::The embryonic dorsal vessel in Drosophila possesses anteroposterior polarity and is subdivided into two chamber-like portions, the aorta in the anterior and the heart in the posterior. The heart portion features a wider bore as compared with the aorta and develops inflow valves (ostia) that allow the pumping of hemoly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0839
更新日期:2002-11-15 00:00:00
abstract::Retinoic acid (RA) signaling is required for normal development of multiple organs. However, little is known about how RA influences the initial stages of lung development. Here, we used a combination of genetic, pharmacological and explant culture approaches to address this issue, and to investigate how signaling by ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.10.045
更新日期:2006-03-01 00:00:00
abstract::Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1068
更新日期:1994-03-01 00:00:00
abstract::Urodeles begin gastrulation with much of their presumptive mesoderm in the superficial cell layer, all of which must move into the deep layers during development. We studied the morphogenesis of superficial mesoderm in the urodeles Ambystoma maculatum, Ambystoma mexicanum, and Taricha granulosa. In all three species, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0718
更新日期:2002-08-15 00:00:00
abstract::Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have ut...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.09.012
更新日期:2004-12-15 00:00:00
abstract::Using an in vitro model of rat epiphyseal chondrocyte differentiation in which cells are maintained in a three-dimensional cell pellet, we show that exogenous TGF-beta 1 reversibly prevents terminal differentiation of epiphyseal chondrocytes into hypertrophic cells. Through maintenance of gene expression for the carti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1200
更新日期:1993-08-01 00:00:00
abstract::Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9727
更新日期:2000-06-15 00:00:00
abstract::Integrins are alphabeta transmembrane receptors that function in key cellular processes, including cell adhesion, differentiation, and extracellular matrix deposition through interactions with extracellular, membrane, and cytoplasmic proteins. We previously identified and cloned a muscle beta1 integrin cytoplasmic bin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00304-x
更新日期:2003-09-01 00:00:00
abstract::Sex in the nematode Caenorhabditis elegans is normally determined by the X chromosome to autosome (X:A) ratio, with XX hermaphrodites and XO males. Previous work has shown that a set of at least four autosomal genes (her-1, tra-2, tra-3, and tra-1) is signaled by the X:A ratio and appears to act in a regulatory pathwa...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90077-0
更新日期:1984-11-01 00:00:00
abstract::The axonal functions that act in the formation of the neuronal network have been shown to occur in close interdependence with the tissue that surrounds the growing axons. However, little is known about the molecular building blocks underlying axonal functions, although more than 400 axonal proteins have been identifie...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90090-4
更新日期:1986-11-01 00:00:00
abstract::We investigated the influence of the growth surface on the direction of Xenopus spinal neurite growth in the presence of a dc electric field of physiological magnitude. The direction of galvanotropism was determined by the substratum; neurites grew toward the negative electrode (cathode) on untreated Falcon tissue cul...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9039
更新日期:1998-11-15 00:00:00
abstract::One of the earliest steps in embryonic development is the establishment of the future body axes. Morphological and molecular data place the Ambulacraria (echinoderms and hemichordates) within the Deuterostomia and as the sister taxon to chordates. Extensive work over the last decades in echinoid (sea urchins) echinode...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.03.030
更新日期:2011-06-01 00:00:00
abstract::Neurogenin (Ngn) 1 and Ngn2 encode basic-helix-loop-helix transcription factors expressed in the developing neocortex. Like other proneural genes, Ngns participate in the specification of neural fates and neuronal identities, but downstream effectors remain poorly defined. We set out to identify Ngn2 effectors in the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.06.013
更新日期:2004-09-15 00:00:00
abstract::Primitive blood constitutes the ventralmost mesoderm in amphibians, and its cleavage-stage origin reveals important clues about the orientation of the dorsal/ventral axis in the embryo. In recent years, investigators employing various lineage-labeling strategies have reported disparate results for the origin of primit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0717
更新日期:2002-08-01 00:00:00
abstract::Axon outgrowth between the spinal cord and the hindlimb of the chick embryo is constrained by three tissues that border axon pathways. Growth cones turn to avoid the posterior sclerotome, perinotochordal mesenchyme, and pelvic girdle precursor during normal development and after experimental manipulation. We wanted to...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(05)80017-x
更新日期:1991-09-01 00:00:00
abstract::The neural cell adhesion molecule (NCAM) is thought to play a role in the formation of the vertebrate nervous system. In mammals and chicken, it is known that more than 100 different forms of the NCAM protein can be generated by alternative splicing of one primary transcript and it is possible that these different for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90276-m
更新日期:1992-01-01 00:00:00
abstract::The release of intracellular calcium (Ca2+) via either inositol 1,4, 5-trisphosphate receptors (IP3R) or ryanodine receptors (RyR) activates a wide variety of signaling pathways in virtually every type of cell. In the present study we demonstrate that at early stages of development IP3R mRNA and functional IP3-gated C...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9120
更新日期:1999-02-15 00:00:00
abstract::Treatment of metaphase II-arrested hamster eggs with activators of protein kinase C has been reported to promote resumption of the cell cycle, second polar body emission, and pronucleus formation (G.I. Gallicano, S.M. Schwarz, R.W. McGaughey, and D.G. Capco, 1993, Dev. Biol. 156, 94-106). In contrast, we have not obse...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1233
更新日期:1995-08-01 00:00:00
abstract::During its development the epidermis of the pf/pf mutant mouse is invaded by cells from the underlying dermis. These invading cells establish a network of cells including fibroblasts, endothelial cells, and nerve fibers, throughout the epidermis. Subsequent to these events the keratohyalin protein, filaggrin, is drast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90194-5
更新日期:1984-04-01 00:00:00
abstract::Skeletal muscles of the avian limb are derived from mononucleated myogenic precursor cells (myoblasts) that migrate into the somatopleural mesoderm of the developing limb bud from the ventrolateral dermomyotome of limb adjacent somites. In the present study, we utilized replication-deficient lacZ-encoding retroviruses...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00028-3
更新日期:2003-01-15 00:00:00
abstract::Cell-cell fusion is a fundamental cellular process that is essential for development as well as fertilization. Myoblast fusion to form multinucleated skeletal muscle myotubes is a well studied, yet incompletely understood example of cell-cell fusion that is essential for formation of contractile skeletal muscle tissue...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.035
更新日期:2009-01-15 00:00:00