当前位置： SCI文献检索 > BONE期刊下所有文献 > Runx2 is required for early stages of endochondral bone formation but delays final stages of bone repair in Axin2-deficient mice.

Runx2 is required for early stages of endochondral bone formation but delays final stages of bone repair in Axin2-deficient mice.

Abstract：

:Runx2 and Axin2 regulate skeletal development. We recently determined that Axin2 and Runx2 molecularly interact in differentiating osteoblasts to regulate intramembranous bone formation, but the relationship between these factors in endochondral bone formation was unresolved. To address this, we examined the effects of Axin2 deficiency on the cleidocranial dysplasia (CCD) phenotype of Runx2(+/-) mice, focusing on skeletal defects attributed to improper endochondral bone formation. Axin2 deficiency unexpectedly exacerbated calvarial components of the CCD phenotype in the Runx2(+/-) mice; the endocranial layer of the frontal suture, which develops by endochondral bone formation, failed to mineralize in Axin2(-/-):Runx2(+/-) mice, resulting in a cartilaginous, fibrotic and larger fontanel than observed in Runx2(+/-) mice. Transcripts associated with cartilage development (e.g., Acan, miR140) were expressed at higher levels, whereas blood vessel morphogenesis transcripts (e.g., Slit2) were suppressed in Axin2(-/-):Runx2(+/-) calvaria. Cartilage maturation was impaired, as primary chondrocytes from double mutant mice demonstrated delayed differentiation and produced less calcified matrix in vitro. The genetic dominance of Runx2 was also reflected during endochondral fracture repair, as both Runx2(+/-) and double mutant Axin2(-/-):Runx2(+/-) mice had enlarged fracture calluses at early stages of healing. However, by the end stages of fracture healing, double mutant animals diverged from the Runx2(+/-) mice, showing smaller calluses and increased torsional strength indicative of more rapid end stage bone formation as seen in the Axin2(-/-) mice. Taken together, our data demonstrate a dominant role for Runx2 in chondrocyte maturation, but implicate Axin2 as an important modulator of the terminal stages of endochondral bone formation.

journal_name

Bone

journal_title

Bone

authors

McGee-Lawrence ME,Carpio LR,Bradley EW,Dudakovic A,Lian JB,van Wijnen AJ,Kakar S,Hsu W,Westendorf JJ

doi

10.1016/j.bone.2014.06.022

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

277-86

eissn

8756-3282

issn

1873-2763

pii

S8756-3282(14)00231-2

journal_volume

66

pub_type

杂志文章

相关文献

BONE文献大全
  • Characterization of the effect of chronic administration of a calcium-sensing receptor antagonist, ronacaleret, on renal calcium excretion and serum calcium in postmenopausal women.

    abstract：:Ronacaleret is an orally-active calcium-sensing receptor (CaSR) antagonist that has the potential for therapeutic utility in the stimulation of PTH release, notably as a bone anabolic agent comparable to recombinant human PTH(1-34) (rhPTH(1-34)). A recent study has shown that, despite the ability to increase circulati...

    journal_title：Bone

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.bone.2013.05.021

    authors： Caltabiano S,Dollery CT,Hossain M,Kurtinecz MT,Desjardins JP,Favus MJ,Kumar R,Fitzpatrick LA

    更新日期：2013-09-01 00:00:00

  • Sex-specific quantitative trait loci contribute to normal variation in bone structure at the proximal femur in men.

    abstract：:Bone structure is an important determinant of osteoporotic fracture. In women, bone structure is highly heritable, and several quantitative trait loci (QTL) have been reported. There are few comparable data in men. This study in men aimed at establishing the heritability of bone structure at the proximal femur, identi...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.05.005

    authors： Peacock M,Koller DL,Lai D,Hui S,Foroud T,Econs MJ

    更新日期：2005-10-01 00:00:00

  • BMPs and the muscle-bone connection.

    abstract：:Muscle and bone are two intimately connected tissues. A coordinated interplay between these tissues at mechanical levels is required for their development, function and ageing. Evidence is emerging that several genes and molecular pathways exert a pleiotropic effect on both muscle and bone. Bone morphogenetic proteins...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2015.05.023

    authors： Sartori R,Sandri M

    更新日期：2015-11-01 00:00:00

  • Determinants of ovine compact bone viscoelastic properties: effects of architecture, mineralization, and remodeling.

    abstract：:Significant decreases in ovine compact bone viscoelastic properties (specifically, stress-rate sensitivity, and damping efficiency) are associated with three years of ovariectomy and are particularly evident at higher frequencies [Proc. Orthop. Res. Soc. 27 (2002) 89]. It is unclear what materials or architectural fea...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2004.04.006

    authors： Les CM,Spence CA,Vance JL,Christopherson GT,Patel B,Turner AS,Divine GW,Fyhrie DP

    更新日期：2004-09-01 00:00:00

  • Time since onset of walking predicts tibial bone strength in early childhood.

    abstract：:Bone strength in adulthood is known to be affected by health at birth and early childhood. Habitual bone loading is a primary determinant of bone strength in later childhood and adulthood. However, the effects of physical activity in early childhood (e.g. crawling, standing and walking) on bone strength are unknown. F...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.08.003

    authors： Ireland A,Rittweger J,Schönau E,Lamberg-Allardt C,Viljakainen H

    更新日期：2014-11-01 00:00:00

  • Distribution of lamellae in human femoral shafts deformed by bending with inferences on mechanical properties.

    abstract：:On the basis of previous investigations indicating that the distribution of osteonic and interstitial lamellae in a normal femur depends on its mechanical properties, a procedure has been devised to provide information on the distribution of lamellae in human femora diaphyses deformed by bending. To achieve this, exac...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(87)90009-3

    authors： Ascenzi A,Improta S,Portigliatti Barbos M,Carando S,Boyde A

    更新日期：1987-01-01 00:00:00

  • Cardiovascular and skeletal safety of zoledronic acid in osteoporosis observational, matched cohort study using Danish and Swedish health registries.

    abstract：BACKGROUND:This observational safety study used national registers to compare the real world cardiovascular and skeletal safety of zoledronic acid (ZA) against oral bisphosphonates (oBP) and untreated population controls. METHODS:Propensity score matched cohort study in Sweden and Denmark. RESULTS:Matched cohort 1 in...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115296

    authors： Rubin KH,Möller S,Choudhury A,Zorina O,Kalsekar S,Eriksen EF,Andersen M,Abrahamsen B

    更新日期：2020-05-01 00:00:00

  • A xenograft model to evaluate the bone forming effects of sclerostin antibody in human bone derived from pediatric osteogenesis imperfecta patients.

    abstract：:Osteogenesis imperfecta (OI) is a rare and severe skeletal dysplasia marked by low bone mass and poor bone quality which is especially burdensome during childhood. Since clinical trials for pediatric OI are difficult, there is a widespread reliance on genetically modified murine models to understand the skeletal effec...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115118

    authors： Surowiec RK,Battle LF,Ward FS,Schlecht SH,Khoury BM,Robbins C,Wojtys EM,Caird MS,Kozloff KM

    更新日期：2020-01-01 00:00:00

  • Effect of modification of hydroxyapatite/collagen composites with sodium citrate, phosphoserine, phosphoserine/RGD-peptide and calcium carbonate on bone remodelling.

    abstract：:This study describes the early interface reaction of cancellous bone to a nanocrystalline hydroxyapatite cement containing type I collagen (HA/Coll) and its modifications with sodium citrate (CI), calcium carbonate (CA), phosphoserine (P) and phosphoserine plus RGD-peptide (RGD). Cylindrical implants of HA/Coll and it...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.11.019

    authors： Schneiders W,Reinstorf A,Pompe W,Grass R,Biewener A,Holch M,Zwipp H,Rammelt S

    更新日期：2007-04-01 00:00:00

  • How can bone turnover modify bone strength independent of bone mass?

    abstract：:The amount of bone turnover in the skeleton has been identified as a predictor of fracture risk independent of areal bone mineral density (aBMD) and is increasingly cited as an explanation for discrepancies between areal bone mineral density and fracture risk. A number of mechanisms have been proposed to explain how b...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2008.02.001

    authors： Hernandez CJ

    更新日期：2008-06-01 00:00:00

  • Microgravity control of autophagy modulates osteoclastogenesis.

    abstract：:Evidence indicates that astronauts experience significant bone loss during space mission. Recently, we used the NASA developed rotary cell culture system (RCCS) to simulate microgravity (μXg) conditions and demonstrated increased osteoclastogenesis in mouse bone marrow cultures. Autophagy is a cellular recycling proce...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.01.004

    authors： Sambandam Y,Townsend MT,Pierce JJ,Lipman CM,Haque A,Bateman TA,Reddy SV

    更新日期：2014-04-01 00:00:00

  • Longitudinal changes in bone mineral density during normal pregnancy.

    abstract：:Pregnancy is a common physiological event that could affect peak bone mass and the risk of developing osteoporosis later in life. There have been few longitudinal studies over a complete reproductive cycle of any size to show whether bone mineral density (BMD) changes. We have measured BMD by dual-energy X-ray absorpt...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(03)00017-6

    authors： Kaur M,Pearson D,Godber I,Lawson N,Baker P,Hosking D

    更新日期：2003-04-01 00:00:00

  • Gene expression profiles give insight into the molecular pathology of bone in primary hyperparathyroidism.

    abstract：:Global gene expression profiling has been used to study the molecular mechanisms of increased bone remodeling caused by PHPT. This disease is a model for chronic over-stimulation of target organs by PTH due to an inappropriate overproduction of the hormone. Hyperactivity of osteoblasts and osteoclasts lead to increase...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.12.020

    authors： Reppe S,Stilgren L,Olstad OK,Brixen K,Nissen-Meyer LS,Gautvik KM,Abrahamsen B

    更新日期：2006-07-01 00:00:00

  • Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration.

    abstract：:In this study we describe the generation and influences on in vitro and in vivo osteogenesis of photo-cured hyaluronic acid (HA) hydrogels loaded with growth and differentiation factor 5 (GDF-5). Prior to loading GDF-5, we characterized the release profiles from these hydrogels and tested their respective cell viabili...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.11.019

    authors： Bae MS,Ohe JY,Lee JB,Heo DN,Byun W,Bae H,Kwon YD,Kwon IK

    更新日期：2014-02-01 00:00:00

  • Effect of ED-71 on modeling of bone in distraction osteogenesis.

    abstract：:We investigated the effect of 2-beta-(3-hydroxypropoxy)-1alpha,25-dihydroxyvitamin D3 (ED-71) on the modeling of bone in distraction osteogenesis. The tibiae of 30 rabbits were lengthened by 10 mm in 10 days. Following osteotomy, ED-71 (0.05 microg/kg) was administered subcutaneously twice a week to the ED-71 group un...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00179-3

    authors： Yamane K,Okano T,Kishimoto H,Hagino H

    更新日期：1999-03-01 00:00:00

  • Disruption of the aldehyde dehydrogenase 2 gene increases the bone anabolic response to intermittent PTH treatment in an ovariectomized mouse model.

    abstract：:Aldehyde dehydrogenase 2 (ALDH2) is the enzyme that oxidizes the acetaldehyde produced by alcohol metabolism. This variant not only affects the response to alcohol but is also associated with several diseases, such as esophageal cancer, myocardial infarction, and particularly osteoporosis. In our previous study, we re...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115370

    authors： Kosugi K,Tajima T,Menuki K,Okuma KF,Tokuda K,Fukuda H,Okada Y,Tsukamoto M,Yamanaka Y,Zenke Y,Sakai A

    更新日期：2020-07-01 00:00:00

  • A novel auditory ossicles membrane and the development of conductive hearing loss in Dmp1-null mice.

    abstract：:Genetic mouse models are widely used for understanding human diseases but we know much less about the anatomical structure of the auditory ossicles in the mouse than we do about human ossicles. Furthermore, current studies have mainly focused on disease conditions such as osteomalacia and rickets in patients with hypo...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2017.06.007

    authors： Lv K,Huang H,Yi X,Chertoff ME,Li C,Yuan B,Hinton RJ,Feng JQ

    更新日期：2017-10-01 00:00:00

  • A Ser252Trp [corrected] substitution in mouse fibroblast growth factor receptor 2 (Fgfr2) results in craniosynostosis.

    abstract：:Apert syndrome (AS) is one of the most severe craniosynostoses and is characterized by premature fusion of craniofacial sutures. Mutations of either Ser252Trp or Pro253Arg in fibroblast growth factor receptor 2 (FGFR2) are responsible for nearly all known cases of AS. Here we show that mutant mice carrying the activat...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(03)00222-9

    authors： Chen L,Li D,Li C,Engel A,Deng CX

    更新日期：2003-08-01 00:00:00

  • Stimulation of osteoinduction in bone wound healing by high-molecular hyaluronic acid.

    abstract：:To study the osteoinductive action of hyaluronic acid (HA), we examined the effects of applying an elastoviscous high-molecular HA preparation on bone wound healing after bone marrow ablation. The middiaphyses of cortical bones from rat femurs were perforated with a round bar, and excavated marrow cavities were filled...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(94)00001-8

    authors： Sasaki T,Watanabe C

    更新日期：1995-01-01 00:00:00

  • Age-related changes in trabecular bone microdamage initiation.

    abstract：:With age, alterations occurring in bone quality, quantity, and microarchitecture affect the resistance of trabecular bone to local failure. The clinical implications of these changes are evident by the observed exponential increase in fracture incidence with age. Although age-related development of skeletal fragility ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.10.028

    authors： Nagaraja S,Lin AS,Guldberg RE

    更新日期：2007-04-01 00:00:00

  • Are effects of MTHFR (C677T) genotype on BMD confined to women with low folate and riboflavin intake? Analysis of food records from the Danish osteoporosis prevention study.

    abstract：:We have previously found BMD and fracture risk to be significantly associated with the MTHFR (C677T) polymorphism in healthy postmenopausal women in the first years after menopause. Since then, other cohort studies have suggested that sufficient intake of riboflavin and/or folate may have the potential to prevent deve...

    journal_title：Bone

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.bone.2004.12.014

    authors： Abrahamsen B,Madsen JS,Tofteng CL,Stilgren L,Bladbjerg EM,Kristensen SR,Brixen K,Mosekilde L

    更新日期：2005-03-01 00:00:00

  • Cell cycle progression is disrupted in murine MPS VII growth plate leading to reduced chondrocyte proliferation and transition to hypertrophy.

    abstract：:Endochondral bone growth is abnormal in 6 of the 11 types of mucopolysaccharidoses (MPS) disorders; resulting in short stature, reduced size of the thoracic cavity and compromised manual dexterity. Current therapies for MPS have had a limited effect on bone growth and to improve these therapies or develop adjunct appr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115195

    authors： Jiang Z,Derrick-Roberts ALK,Reichstein C,Byers S

    更新日期：2020-03-01 00:00:00

  • Vitamin D receptor polymorphism, bone mineral density, and osteoporotic vertebral fracture: studies in a UK population.

    abstract：:Bone mineral density is under strong genetic control and polymorphisms of the vitamin D receptor (VDR) have been suggested to account for some of the genetic variation in bone mass. However, the relationship between VDR polymorphisms and bone density is controversial and has not been confirmed by all workers. Since th...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(95)00483-1

    authors： Houston LA,Grant SF,Reid DM,Ralston SH

    更新日期：1996-03-01 00:00:00

  • Fracture toughness of human femoral neck: effect of microstructure, composition, and age.

    abstract：:The effects of porosity and pore size; osteonal area, size, and density; mineral content; water content; wet and dry apparent densities; and age on mode I (tensile) and mode II (shear) strain energy release rate were investigated for femoral neck cortical bone from human cadavers aged >/=50 years. The results suggest ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/S8756-3282(00)00258-1

    authors： Yeni YN,Norman TL

    更新日期：2000-05-01 00:00:00

  • The role of endogenous GIP and GLP-1 in postprandial bone homeostasis.

    abstract：:The incretin hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP-1) are well known for their insulinotropic effects and they are thought to affect bone homeostasis as mediators in the so-called entero-osseous axis. We examined the contributions of endogenous GIP and GLP-1, resp...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115553

    authors： Helsted MM,Gasbjerg LS,Lanng AR,Bergmann NC,Stensen S,Hartmann B,Christensen MB,Holst JJ,Vilsbøll T,Rosenkilde MM,Knop FK

    更新日期：2020-11-01 00:00:00

  • Orthopedic complications in diabetes.

    abstract：:Diabetes is associated with a number of lower extremity orthopedic conditions and complications including fractures, Charcot neuroarthropathy, plantar ulcers, and infection. These complications are of significant clinical concern in terms of morbidity, mortality, and socioeconomic costs. A review of each condition is ...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2015.07.029

    authors： Gehling DJ,Lecka-Czernik B,Ebraheim NA

    更新日期：2016-01-01 00:00:00

  • Positive effect of low dose vitamin D supplementation on growth of fetal bones: A randomized prospective study.

    abstract：:The effect of vitamin D supplementation on growth of fetal bones during pregnancy is unclear. The aim of this study was to assess the effect of low dose vitamin D supplementation during pregnancy on bony anthropometric aspects of the fetus. In this prospective randomized trial, 140 patients were divided into two equal...

    journal_title：Bone

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.bone.2019.02.022

    authors： Vafaei H,Asadi N,Kasraeian M,Shahraki HR,Bazrafshan K,Namazi N

    更新日期：2019-05-01 00:00:00

  • High levels of serum IL-18 promote cartilage loss through suppression of aggrecan synthesis.

    abstract：:Osteoarthritis (OA) is closely related to the function of several inflammatory cytokines. It has been reported that older age is associated with higher serum levels of the inflammatory cytokine IL-18. In the present study, we investigated the long-term role of serum IL-18 in cartilage loss in vivo using a new strain o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.01.031

    authors： Inoue H,Hiraoka K,Hoshino T,Okamoto M,Iwanaga T,Zenmyo M,Shoda T,Aizawa H,Nagata K

    更新日期：2008-06-01 00:00:00

  • Effects of teriparatide on cortical histomorphometric variables in postmenopausal women with or without prior alendronate treatment.

    abstract：:Cortical bone, the dominant component of the human skeleton by volume, plays a key role in protecting bones from fracture. We analyzed the cortical bone effects of teriparatide treatment in postmenopausal women with osteoporosis who had previously received long-term alendronate (ALN) therapy or were treatment naïve (T...

    journal_title：Bone

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.bone.2013.11.011

    authors： Ma YL,Zeng QQ,Chiang AY,Burr D,Li J,Dobnig H,Fahrleitner-Pammer A,Michalská D,Marin F,Pavo I,Stepan JJ

    更新日期：2014-02-01 00:00:00

  • Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects.

    abstract：:The temporal expression of bone microsomal casein kinase II, osteopontin, bone sialoprotein, alkaline phosphatase, and the accumulation of a solid calcium-inorganic orthophosphate mineral phase, have been charted from day 2 to day 21 during the repair of calvarial defects in rats induced by the implantation of decalci...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00057-x

    authors： Wang J,Glimcher MJ,Mah J,Zhou HY,Salih E

    更新日期：1998-06-01 00:00:00