Combinatory RNA-Sequencing Analyses Reveal a Dual Mode of Gene Regulation by ADAR1 in Gastric Cancer.

Abstract:

BACKGROUND:Adenosine deaminase acting on RNA 1 (ADAR1) is known to mediate deamination of adenosine-to-inosine through binding to double-stranded RNA, the phenomenon known as RNA editing. Currently, the function of ADAR1 in gastric cancer is unclear. AIMS:This study was aimed at investigating RNA editing-dependent and editing-independent functions of ADAR1 in gastric cancer, especially focusing on its influence on editing of 3' untranslated regions (UTRs) and subsequent changes in expression of messenger RNAs (mRNAs) as well as microRNAs (miRNAs). METHODS:RNA-sequencing and small RNA-sequencing were performed on AGS and MKN-45 cells with a stable ADAR1 knockdown. Changed frequencies of editing and mRNA and miRNA expression were then identified by bioinformatic analyses. Targets of RNA editing were further validated in patients' samples. RESULTS:In the Alu region of both gastric cell lines, editing was most commonly of the A-to-I type in 3'-UTR or intron. mRNA and protein levels of PHACTR4 increased in ADAR1 knockdown cells, because of the loss of seed sequences in 3'-UTR of PHACTR4 mRNA that are required for miRNA-196a-3p binding. Immunohistochemical analyses of tumor and paired normal samples from 16 gastric cancer patients showed that ADAR1 expression was higher in tumors than in normal tissues and inversely correlated with PHACTR4 staining. On the other hand, decreased miRNA-148a-3p expression in ADAR1 knockdown cells led to increased mRNA and protein expression of NFYA, demonstrating ADAR1's editing-independent function. CONCLUSIONS:ADAR1 regulates post-transcriptional gene expression in gastric cancer through both RNA editing-dependent and editing-independent mechanisms.

journal_name

Dig Dis Sci

authors

Cho CJ,Jung J,Jiang L,Lee EJ,Kim DS,Kim BS,Kim HS,Jung HY,Song HJ,Hwang SW,Park Y,Jung MK,Pack CG,Myung SJ,Chang S

doi

10.1007/s10620-018-5081-9

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

1835-1850

issue

7

eissn

0163-2116

issn

1573-2568

pii

10.1007/s10620-018-5081-9

journal_volume

63

pub_type

杂志文章
  • Distal gastrectomy and Roux-Y limb in the rat: plasma motilin, pancreatic polypeptide concentrations, and duodenojejunal motility.

    abstract::After gastrectomy, Roux-Y limb reconstruction leads to duodenojejunal motor disturbances. Because motilin and pancreatic polypeptide (PP) play a role in the regulation of digestive motility, their plasma concentrations were determined in rats after Roux-Y gastrectomy. Three months after a distal Roux-Y gastrectomy, co...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1023/a:1005548524334

    authors: Le Blanc-Louvry I,Ducrotte P,Chayvialle JA,Coquerel A,Michot F,Ténière P

    更新日期:2000-08-01 00:00:00

  • Pancreaticopleural fistula: report of two cases and review of the literature.

    abstract::Pancreaticopleural fistulas are a rare complication of pancreatitis. We report two cases from our institution and review 37 cases of pancreaticopleural fistulas identified in the literature. Endoscopic retrograde cholangiopancreatography was more sensitive compared to computed tomography in demonstrating pancreaticopl...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-006-3073-7

    authors: Oh YS,Edmundowicz SA,Jonnalagadda SS,Azar RR

    更新日期:2006-01-01 00:00:00

  • Prognostic index for survival in patients after treatment for primary hepatocellular carcinoma.

    abstract::We retrospectively evaluated clinical factors affecting long-term survival after treatment for hepatocellular carcinoma (HCC) to predict the reliability of the prognosis of patients with HCC. We analyzed 157 patients who received treatment for HCC. The prognostic index (PI) was evaluated using the Cox regression model...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-006-9137-x

    authors: Kogo M,Kano A,Kiuchi Y,Mitamura K,Yoneyama K

    更新日期:2007-09-01 00:00:00

  • Ornithine decarboxylase activity during gastric ulcer healing in dogs.

    abstract::Ornithine decarboxylase (ODC) activity has been associated with mucosal growth and injury, yet, little information is available on ODC activity during gastric ulcer healing. We measured ODC activity in the ulcer base submucosa and the surrounding mucosa at 1 cm and 2 cm and assessed ulcer surface healing and a histolo...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01300280

    authors: Marcuard SP,Silverman JF,Finley JL,Seidel ER

    更新日期:1992-07-01 00:00:00

  • Reduction of virus shedding by B. bifidum in experimentally induced MRV infection. Statistical application for ELISA.

    abstract::The protective effect of a human strain of Bifidobacterium bifidum (B. bifidum) against murine group A rotavirus (MRV) was examined in the intestines of BALB/c infected mice. In experiments designed to determine whether B. bifidum mediated MRV shedding during diarrheal disease, pregnant dams (and their expected litter...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF02087647

    authors: Duffy LC,Zielezny MA,Riepenhoff-Talty M,Dryja D,Sayahtaheri-Altaie S,Griffiths E,Ruffin D,Barrett H,Ogra PL

    更新日期:1994-11-01 00:00:00

  • Nitric oxide synthase and VIP distribution in enteric nervous system in idiopathic chronic constipation.

    abstract::Idiopathic chronic constipation has been correlated to neural abnormalities that consist of a reduced number of myenteric plexus neurons and a decreased concentration of VIP-positive nerve fibers within the circular muscle. Recent studies hypothesized the involvement of nitric oxide in motility disorders of the human ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF02063253

    authors: Cortesini C,Cianchi F,Infantino A,Lise M

    更新日期:1995-11-01 00:00:00

  • Dissolution of cholesterol ductal stones in the biliary tree with medium-chain glycerides.

    abstract::In vitro and in vivo experiments were carried out with medium-chain glycerides. In vitro, cholesterol gallstones were rapidly dissolved by medium-chain glycerides; control experiments with saline solution failed to modify the size of the stones. In vivo, medium-chain glycerides were instilled through an intraductal tu...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01367677

    authors: Uribe M,Uscanga L,Farca S,Sanjurjo JL,LaGarriga J,Ortíz JH

    更新日期:1981-07-01 00:00:00

  • Hydrogen breath test quantification and clinical correlation of lactose malabsorption in adult irritable bowel syndrome and ulcerative colitis.

    abstract::Lactose malabsorption was studied, by hydrogen breath test, in 72 adults suffering from irritable bowel syndrome, in 20 ulcerative colitis patients, and in 69 healthy subjects. The minimum dose of lactose required to cause a positive breath test was determined, and the symptoms caused and the resulting hydrogen elimin...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01317083

    authors: Sciarretta G,Giacobazzi G,Verri A,Zanirato P,Garuti G,Malaguti P

    更新日期:1984-12-01 00:00:00

  • In Vitro and In Vivo Renoprotective Effects of Telbivudine in Chronic Hepatitis B Patients Receiving Nucleotide Analogue.

    abstract:AIM:Renal toxicity of adefovir disoproxil (ADV) and tenofovir disoproxil fumarate (TDF) is a significant concern in chronic hepatitis B (CHB) patients. Early observational clinical data suggested that telbivudine (LdT) might have renoprotective effects. METHODS:In this prospective study, consecutive CHB patients on co...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-019-05717-0

    authors: Mak LY,Liu SH,Yap DY,Seto WK,Wong DK,Fung J,Chan TM,Lai CL,Yuen MF

    更新日期:2019-12-01 00:00:00

  • Reduction in hMSH2 mRNA levels by premature translation termination: implications for mutation screening in hereditary nonpolyposis colorectal cancer.

    abstract::We have used single-strand conformational polymorphism (SSCP) and heteroduplex analysis to examine DNA from 50 colorectal carcinoma patients coming from families meeting the Amsterdam criteria for hereditary nonpolyposis colorectal cancer (HNPCC) or having developed colorectal carcinoma at age 45 or younger. We identi...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1023/a:1026609524482

    authors: Lin X,Choi JH,Lynch P,Xi L,Wu E,Frazier ML

    更新日期:1999-03-01 00:00:00

  • SEL1L, an UPR response protein, a potential marker of colonic cell transformation.

    abstract:BACKGROUND:SEL1L gene product is implicated in the endoplasmic reticulum (ER)-associated protein degradation and Unfolded Protein Response pathways. This gene and associated miRNAs have been indicated as predictive and prognostic markers of pancreatic cancer. AIM:Explore the role of SEL1L in colorectal cancer (CRC) pr...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-011-2026-y

    authors: Ashktorab H,Green W,Finzi G,Sessa F,Nouraie M,Lee EL,Morgano A,Moschetta A,Cattaneo M,Mariani-Costantini R,Brim H,Biunno I

    更新日期:2012-04-01 00:00:00

  • Inhibitory effect of small amounts of cellulose on colonic carcinogenesis with low-dose carcinogen.

    abstract::This study set out to evaluate the effects of dietary fiber on cancer development in the large bowel under in vivo experimental conditions as similar as possible to those under which this cancer develops in vivo in humans. Forty-eight Sprague-Dawley rats were divided into three groups that were fed either a nonfiber d...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1023/a:1015310211821

    authors: Iwane S,Nakaji S,Ohta M,Tamura K,Sakamoto J,Munakata A,Sugawara K

    更新日期:2002-06-01 00:00:00

  • Patterns of Gram-stained fecal flora as a quick diagnostic marker in patients with severe SIRS.

    abstract:BACKGROUND:The gut is an important target organ of injury during critically ill conditions. Although Gram staining is a common and quick method for identifying bacteria, its clinical application has not been fully evaluated in critically ill conditions. AIMS:This study's aims were to identify patterns of Gram-stained ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-010-1486-9

    authors: Shimizu K,Ogura H,Tomono K,Tasaki O,Asahara T,Nomoto K,Morotomi M,Matsushima A,Nakahori Y,Yamano S,Osuka A,Kuwagata Y,Sugimoto H

    更新日期:2011-06-01 00:00:00

  • Artificial liver. State of the art.

    abstract::If an effective hepatic assist system existed, it could serve as a bridge to transplantation. Most of the patients waiting for liver transplantation have chronic liver insufficiency but are not in hepatic coma. Various hepatic assist systems have been used to salvage patients with acute liver insufficiency. Most attem...

    journal_title:Digestive diseases and sciences

    pub_type: 历史文章,杂志文章,评审

    doi:10.1007/BF01307531

    authors: Takahashi T,Malchesky PS,Nosé Y

    更新日期:1991-09-01 00:00:00

  • Association of adult achalasia and alacrima.

    abstract::Achalasia is the best described motor disorder of the esophagus. Associations with familial glucocorticoid deficiency and alacrima (triple A syndrome) have been described in the pediatric population, but no attention has been paid to the possibility of this association in adults. Tear production was assessed in 20 pat...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1023/a:1026679723941

    authors: Verma S,Brown S,Dakkak M,Bennett JR

    更新日期:1999-05-01 00:00:00

  • Gallbladder mucosal protein secretion during development of experimental cholecystitis.

    abstract::The development of experimental cholecystitis produced by lysophosphatidylcholine is associated with reversal of the normal absorptive characteristics of gallbladder mucosa, resulting in the intraluminal accumulation of water, glycoprotein, and protein. The purpose of the present study was to attempt to ascertain if t...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF02064215

    authors: Kaminski DL,Deshpande YG,Li A,Dysart F,Nag M

    更新日期:1995-05-01 00:00:00

  • Different interdigestive antroduodenal motility patterns in chronic antral gastritis with and without Helicobacter pylori infection.

    abstract::Fasting antroduodenal motor activity was studied in 15 dyspeptic patients with chronic superficial antral gastritis and Helicobacter pylori infection (group A), 10 dyspeptic patients with chronic superficial antral gastritis without Helicobacter pylori infection (group B), and eight healthy control subjects (group C) ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01299905

    authors: Testoni PA,Bagnolo F,Masci E,Colombo E,Tittobello A

    更新日期:1993-12-01 00:00:00

  • Antibacterial action of selenium-enriched probiotics against pathogenic Escherichia coli.

    abstract::The purpose of this study was to evaluate the inhibitory activity of selenium-enriched probiotics against pathogenic Escherichia coli (E. coli) in vitro and in vivo. Escherichia coli was co-cultured in vitro with each probiotic strain individually, and a mixture of the four strains and its population was counted at va...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-008-0361-4

    authors: Yang J,Huang K,Qin S,Wu X,Zhao Z,Chen F

    更新日期:2009-02-01 00:00:00

  • Can endoscopic ultrasound-guided fine needle aspiration offer clinical benefit for thick-walled gallbladders?

    abstract:BACKGROUND:No previous studies have compared cytology obtained under endoscopic transpapillary gallbladder drainage (ETGD) and EUS-guided fine needle aspiration (EUS-FNA) for thick-walled gallbladders. AIM:The present study investigated the diagnostic yield of bile cytology under ETGD and EUS-FNA for gallbladder tumor...

    journal_title:Digestive diseases and sciences

    pub_type: 临床试验,杂志文章

    doi:10.1007/s10620-014-3100-z

    authors: Ogura T,Kurisu Y,Masuda D,Imoto A,Onda S,Kamiyama R,Hayashi M,Mohamed M,Uchiyama K,Higuchi K

    更新日期:2014-08-01 00:00:00

  • Peritoneal sarcoidosis. An unrecognized cause of sclerosing peritonitis.

    abstract::In a Caucasian woman with a history of ocular and pulmonary sarcoidosis, the occurrence of sclerosing peritonitis with exudative ascites but without any of the well-known causes of this syndrome prompts us to consider that sclerosing peritonitis is a manifestation of sarcoidosis. The dramatic improvement that occurred...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01299875

    authors: Ngô Y,Messing B,Marteau P,Nouël O,Pasquiou A,Lavergne A,Rambaud JC

    更新日期:1992-11-01 00:00:00

  • Anomalous plasma cyclic AMP responses to glucagon in patients with liver disease.

    abstract::The purpose of the present study is to show anomalies of the plasma cAMP response of patients with hepatic disorders to a single injection of a low dose of glucagon (1 microgram/kg body wt). The response was markedly blunted in patients with liver cirrhosis and potentiated in patients with acute or chronic hepatitis. ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01308330

    authors: Maekubo H,Matsushima T,Okada F,Honma M,Ui M

    更新日期:1980-09-01 00:00:00

  • Correlation between biliary alpha 1-acid glycoprotein concentration and cholesterol crystal nucleation time in gallstone disease.

    abstract::A biliary form of the alpha 1-acid glycoprotein (AAG) promotes cholesterol crystallization in the lower-molecular-weight, concanavalin A-bound fraction of gallbladder bile. In addition, bile AAG concentration is higher in cholesterol gallstone patients with multiple stones than in control patients without gallstone di...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF02065520

    authors: Nuutinen H,Ginanni Corradini S,Jüngst D,Lange V,Abei M,Schwarzendrube J,Williams C,Holzbach RT

    更新日期:1995-06-01 00:00:00

  • Clinical patterns, natural history, and progression of ulcerative colitis. A long-term follow-up of 1116 patients.

    abstract::We studied the natural history of ulcerative colitis (UC) by following 1116 patients in whom UC had been diagnosed or confirmed at The Cleveland Clinic Foundation between 1960 and 1983. Data before 1973 were obtained retrospectively. Criteria for inclusion in the study were: a diagnosis of UC confirmed by clinical, ra...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01295733

    authors: Farmer RG,Easley KA,Rankin GB

    更新日期:1993-06-01 00:00:00

  • Aspirin may prevent cholangiocarcinoma: a case-control study from the United kingdom.

    abstract:BACKGROUND:The proliferation of cholangiocarcinoma cells is suppressed in cell culture by nonsteroidal antiinflammatory drugs (NSAIDs) through the inhibition of cyclo-oxygenase-2 enzyme and also by statins which decrease the production of mediators of the cell cycle. AIMS:To investigate whether there is an inverse ass...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-014-3056-z

    authors: Burr NE,Talboys RJ,Savva S,Clark A,Phillips M,Metcalfe M,Dennison A,Robinson R,Lewis MP,Rhodes M,Rushbrook S,Hart AR

    更新日期:2014-07-01 00:00:00

  • Loss of matrix metalloproteinase-2 amplifies murine toxin-induced liver fibrosis by upregulating collagen I expression.

    abstract:BACKGROUND AND AIMS:Matrix metalloproteinase-2 (MMP-2), a type IV collagenase secreted by activated hepatic stellate cells (HSCs), is upregulated in chronic liver disease and is considered a profibrotic mediator due to its proliferative effect on cultured HSCs and ability to degrade normal liver matrix. Although associ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/s10620-010-1296-0

    authors: Radbill BD,Gupta R,Ramirez MC,DiFeo A,Martignetti JA,Alvarez CE,Friedman SL,Narla G,Vrabie R,Bowles R,Saiman Y,Bansal MB

    更新日期:2011-02-01 00:00:00

  • Prospective, multicenter study on value of computerized tomography (CT) in gallstone disease in predicting response to bile acid therapy.

    abstract::The aim of the study was to assess the value of quantitative attenuation values (Hounsfield units) and of gallstone pattern by computerized tomography in predicting response to bile acid therapy. We carried out a prospective study in a multicenter setting on 90 consecutive outpatients with radiolucent gallstones. All ...

    journal_title:Digestive diseases and sciences

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1007/BF02208664

    authors: Petroni ML,Jazrawi RP,Grundy A,Lanzini A,Pigozzi MG,Biasio A,Heaton KW,Virjee J,Northfield TC

    更新日期:1995-09-01 00:00:00

  • Human beta-glucuronidase. Measurement of its activity in gallbladder bile devoid of intrinsic interference.

    abstract::Our purpose is to develop a standard method for preparing the bile for beta-glucuronidase determination by removal of bile acids and conjugated bilirubin which interfere with its activity. The bile acids and conjugated bilirubin in their purified solutions and in the diluted gallbladder biles could be extracted comple...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01536028

    authors: Ho YC,Ho KJ

    更新日期:1988-04-01 00:00:00

  • Hepatitis C virus infection in institutionalized psychiatric patients: possible role of transmission by razor sharing.

    abstract::The objective of this study was to determine if HCV can be transmitted from patient to patient in psychiatric institutions and to determine possible routes of infection. We did a cross-sectional survey of 196 Japanese psychiatric patients tested for HCV and HBV markers and 400 age- and sex-matched controls. Anti-HCV w...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1023/a:1005472812403

    authors: Sawayama Y,Hayashi J,Kakuda K,Furusyo N,Ariyama I,Kawakami Y,Kinukawa N,Kashiwagi S

    更新日期:2000-02-01 00:00:00

  • Insulin and myoelectric activity of the small intestine of the pig.

    abstract::The effect of insulin on the myoelectric activity of the small intestine was determined in conscious pigs. Animals were implanted with electrodes along the small intestine, a strain gage on the stomach and catheters in both saphenous arteries. Feeding modified the migrating myoelectric complex (MMC), a cyclic pattern ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF01307973

    authors: Rayner V,Weekes TE,Bruce JB

    更新日期:1981-01-01 00:00:00

  • Gastroparesis of digestible and indigestible solids in patients with insulin-dependent diabetes mellitus or functional dyspepsia.

    abstract::Gastric emptying of a solid meal and of 10 indigestible radiopaque solids was measured with scintigraphic and radiological techniques in 50 healthy volunteers (controls), 41 patients with insulin-dependent diabetes mellitus, and 50 patients with functional dyspepsia. Gastroparesis was found in 51% of our diabetic pati...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章

    doi:10.1007/BF02088042

    authors: Caballero-Plasencia AM,Muros-Navarro MC,Martin-Ruiz JL,Valenzuela-Barranco M,de los Reyes-Garcia MC,Vilchez-Joya R,Casado-Caballero FJ,Gil-Extremera B

    更新日期:1994-07-01 00:00:00