Heterogeneity of BCR-ABL rearrangement in patients with chronic myeloid leukemia in Pakistan.

Abstract:

BACKGROUND AND OBJECTIVE:Breakpoint cluster region-Abelson (BCR-ABL) rearrangement or Philadelphia (Ph) chromosome in Chronic Myeloid Leukemia (CML) is derived from a reciprocal chromosomal translocation between ABL gene on chromosome 9 and BCR gene on chromosome 22. This chimeric protein has various sizes and therefore different clinical behaviour. The purpose of this study was to determine the heterogeneity of BCR-ABL rearrangement in patients with Ph(+)CML in Pakistan. METHODS:The study was conducted at Civil Hospital and Baqai Institute of Hematology (BIH) Karachi. Blood samples from 25 patients with CML were collected. Multiplex reverse transcription polymerase chain reaction (RT-PCR) was performed to identify various BCR-ABL transcripts. RESULTS:All 25 samples showed BCR-ABL rearrangements. Out of these, 24 (96%) patients expressed p210 BCR-ABL rearrangements i.e. 60% (n=15) had b3a2 and 32% (n=8) had b2a2 rearrangements. Co-expression of b3a2 /b2a2 rearrangement and p190 (e1a3) rearrangement was also identified in two patients. CONCLUSION:It is apparent that majority of the patients had p210 BCR-ABL rearrangements. Frequency of co-expression and rare fusion transcripts was very low.

journal_name

Pak J Med Sci

authors

Tabassum N,Saboor M,Ghani R,Moinuddin M

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

850-3

issue

4

eissn

1682-024X

issn

1681-715X

journal_volume

30

pub_type

杂志文章
  • Improvement in JOA Score of Treatment for Complex Atlas-Axis fractures.

    abstract:OBJECTIVE:The aim of this study was to explore the role of treatment for complex Atlas-Axis fractures, and compare the JOA score of surgical and conservation methods. METHODOLOGY:From June 2008 to May 2012, 33 patients suffering from Atlas-Axis fracture were included in our study. Fifteen patients received posterior c...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.293.2995

    authors: Fu Y,Hu ZM,Huo HJ,Yang X,Hao T,Liu WL

    更新日期:2013-05-01 00:00:00

  • Allergic Fungal rhino-sinusitis frequency in chronic rhino-sinusitis patients and accuracy of fungal culture in its diagnosis.

    abstract:Objective:To determine the frequency of Allergic Fungal Rhino-sinusitis (AFRS) in Chronic Rhino-sinusitis (CRS) patients and the accuracy of fungal culture in diagnosing AFRS. Methods:Immunocompetent patients with CRS and without invasive fungal rhino-sinusitis presenting over a period of 3 years in ENT department of ...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.3.1661

    authors: Awan NU,Cheema KM,Naumeri F,Qamar S

    更新日期:2020-03-01 00:00:00

  • Clinical exploration of marking targeting biopsy in the intraoperative localization value of colon polypectomy.

    abstract:Objective:To evaluate the feasibility and safety of marking targeting biopsy (MTB) in the intraoperative localization value of colon polypectomy. Methods:The clinical data from patients with polyp of colon discovered under colonoscopy from January 2014 to January 2016 were retrospectively analyzed. A total of 87 patie...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.2.756

    authors: Song LY,Lin Q,Li LB,Cheng X

    更新日期:2020-01-01 00:00:00

  • Comparison of failure loads and compressive stress in Press on metal and Press on Y-TZP copings.

    abstract:Objective:The aim of the study was to assess the failure loads and compressive stresses among bilayered press on Y-TZP (POZ) and press on metal (POM) crowns with different core-veneer thickness. Methods:Thirty metal and Y-TZP copings were fabricated using CAD-CAM technology with specified thickness. All copings were v...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.7.2472

    authors: Vohra F,Shuwaish MB,Deeb MA,Alhamdan R,Alotaibi N,Abduljabbar T

    更新日期:2020-11-01 00:00:00

  • Cardiovascular risk factors among retired attendees visiting primary care clinics.

    abstract:OBJECTIVE:The aim of this study was to highlight cardiovascular risk factors among retired attendees attending a primary care clinic, Riyadh, Saudi Arabia. METHODS:A cross sectional study was conducted from Januaryto February 2013 at Primary Care Clinics of King Khalid University Hospital and College of Medicine, King...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.303.4269

    authors: Al Turki YA

    更新日期:2014-05-01 00:00:00

  • Glial Fibrillary Acidic Protein (GFAP) as a Mesenchymal marker of Early Hepatic Stellate Cells Activation in Liver Fibrosis in Chronic Hepatitis C Infection.

    abstract:OBJECTIVE:This study aims to determine expression of Glial Fibrillary Acidic Protein and of Alpha Smooth Muscle Actin (α-SMA) in hepatic stellate cells of CHC cases and their association with stage of fibrosis. METHODS:The study was conducted at Ziauddin University, Clifton Campus during the year 2010-2012. Sixty Chro...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.305.5534

    authors: Hassan S,Syed S,Kehar SI

    更新日期:2014-09-01 00:00:00

  • Potential effects of traditional massage on spasticity and gross motor function in children with spastic cerebral palsy: A randomized controlled trial.

    abstract:Objective:To evaluate the effects of traditional massage (TM) on spasticity and gross motor function in children with cerebral palsy (CP). Methods:This randomized control trial (RCT) was conducted with recruitment of 86 children (Dropped out= 11; Analyzed= 75) with spastic CP (diplegia) allocated randomly through seal...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.35.5.478

    authors: Mahmood Q,Habibullah S,Babur MN

    更新日期:2019-09-01 00:00:00

  • Medical Education in Iraq: The cradle of Civilisation.

    abstract::Medical education is in state of flux in Iraq. What used to be the best physicians and surgeons in the Arab world have fallen down to much lower status due to deliberate neglect and punishment of the intellectuals and professionals by the previous authorities. Once again medical educators and health professional are g...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.35.3.972

    authors: Zaidi SH,Abutiheen A

    更新日期:2019-01-01 00:00:00

  • Comparison of tumor curettage and resection for treatment of giant cell tumor of the bone around the knee joint.

    abstract:OBJECTIVE:To analyze the efficacies of tumor curettage and resection for treatment of giant cell tumor of the bone (GCTB) around the knee joint (KJ). METHODS:A total of 126 KJ-GCTB cases were treated at our department from August 2011 to February 2015. These cases were divided into two groups (A and B) according to tr...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.323.9654

    authors: Zhang S,Zhang J,Wang X

    更新日期:2016-05-01 00:00:00

  • Advantages, Limitations and Recommendations for online learning during COVID-19 pandemic era.

    abstract:Objective:During COVID-19 pandemic, the institutions in Pakistan have started online learning. This study explores the perception of teachers and students regarding its advantages, limitations and recommendations. Methods:This qualitative case study was conducted from March to April 2020. Using maximum variation sampl...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.COVID19-S4.2785

    authors: Mukhtar K,Javed K,Arooj M,Sethi A

    更新日期:2020-05-01 00:00:00

  • Association between 25-Hydroxy Vitamin D and volumetric breast density via a fully automated software Volpara™ in the reproductive age group.

    abstract:OBJECTIVE:To determine the association between serum 25 hydroxyvitamin D levels and percent breast density among asymptomatic premenopausal women. METHODS:Hundred asymptomatic, pre-menopausal women who visited the General Surgery Breast Clinic, Patel Hospital, Karachi, Pakistan between 3rd March and 10th November, 201...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.325.10731

    authors: Wasim B,Khan K,Samad MA

    更新日期:2016-09-01 00:00:00

  • Risk factors for acquisition of ventilator-associated pneumonia in adult intensive care units.

    abstract:OBJECTIVE:Ventilator Associated Pneumonia (VAP) has an imperative place amongst nosocomial infections leading to increase morbidity and mortality rates. The present study aimed to determine risk factors for acquisition of ventilator- associated pneumonia in an intensive care unit (ICU). METHODS:A nested case-control s...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.295.3375

    authors: Lahoorpour F,Delpisheh A,Afkhamzadeh A

    更新日期:2013-09-01 00:00:00

  • Knowledge, Misconceptions and Motivations Towards Blood Donation Among University Students in KSA.

    abstract:OBJECTIVE:To determine the knowledge, misconceptions and motivations towards blood donation among university students in KSA. METHODS:This cross sectional study was carried out at the King Abdulaziz University, Rabigh campus, Jeddah, KSA. A total of 326 adult males were interviewed and each individual completed a ques...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.296.4137

    authors: Baig M,Habib H,H Haji A,T Alsharief F,M Noor A,G Makki R

    更新日期:2013-11-01 00:00:00

  • Prevalence of Atherosclerosis in diabetic and non-diabetic patients with rheumatoid arthritis.

    abstract:OBJECTIVES:(1) To compare the prevalence of preclinical atherosclerosis in diabetic vs. non-diabetic rheumatoid arthritis (RA) patients; (2) to determine the influence of classical and RA-related factors on atherosclerosis; (3) to assess the usefulness of combined carotid and femoral ultrasonography in detecting athero...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.315.7620

    authors: Kisiel B,Kruszewski R,Juszkiewicz A,Kłos K,Tłustochowicz M,Tłustochowicz W

    更新日期:2015-09-01 00:00:00

  • Diagnostic accuracy of NMP 22 and urine cytology for detection of transitional cell carcinoma urinary bladder taking cystoscopy as gold standard.

    abstract:Objective:To determine diagnostic accuracy of NMP 22 and urine cytology in the detection of transitional cell carcinoma (TCC) urinary bladder taking cystoscopy as a gold standard in patients having provisional diagnosis of bladder cancer (BC). Methods:This cross sectional validational study enrolled 380 patients fulfi...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.4.1638

    authors: Sajid MT,Zafar MR,Ahmad H,Ullah S,Mirza ZI,Shahzad K

    更新日期:2020-05-01 00:00:00

  • Relationship between myocardial enzyme levels, hepatic function and metabolic acidosis in children with rotavirus infection diarrhea.

    abstract:Objective:To investigate the relationship between myocardial enzymes, liver function and metabolic acidosis in children with rotavirus infection diarrhea. Methods:The data of 70 children with infectious diarrhea treated in Baoding Children's Hospital, China, from October 2017 to April 2018 were retrospectively studied...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.6.2325

    authors: Zuo NY,Zhang YD,Dong QW,Han LP

    更新日期:2020-09-01 00:00:00

  • The relationship between dispositional empathy and prefrontal cortical functioning in patients with frontal lobe epilepsy.

    abstract:BACKGROUND & OBJECTIVE:Rehabilitation focuses brain-behavior relationship which highlights interaction between psychological and neurobiological factors for better patient care. There is a missing link in the literature about socio-cognitive aspects of frontal lobe epilepsy. Our objective was to examine prefrontal cort...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.331.11742

    authors: Gul A,Ahmad H

    更新日期:2017-01-01 00:00:00

  • Hepatitis B, Hepatitis C and HIV seroprevalence in critically ill emergency medicine department patients in a tertiary inner city hospital in Istanbul, Turkey.

    abstract:OBJECTIVE:Emergency medicine staff is working at risk of blood-borne infections during their daily practice every time. The risk of transmission is higher when dealing with critically ill patients. Our objective was to find out the prevalence of Hepatitis B, Hepatitis C, and HIV, in critically ill red-coded emergency d...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.304.4975

    authors: Ozturk TC,Guneysel O,Tali A,Yildirim SE,Onur OE,Yaylaci S

    更新日期:2014-07-01 00:00:00

  • Mixed method survey to assess the problems and propose solutions for implementation of CME/CPD in Sindh, Pakistan.

    abstract:Objectives:To determine the type of CME/CPD sessions attended by doctors, identify the problems with implementation of CME/CPD program and propose possible solutions for successful implementation of CME/CPD Program. Methods:This was a concurrent triangulation mixed method study. Quantitative questionnaires were filled...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.35.2.243

    authors: Shah N,Soomro M,Musharraf M,Shaikh S

    更新日期:2019-03-01 00:00:00

  • Analysis of liver function test abnormalities in kidney transplant recipients: 7 year experience.

    abstract:OBJECTIVE:Immunosuppressive drugs, antimicrobial agents and infectious complications may cause liver function test abnormalities (LFTA) in kidney transplant recipients (KTR). The objectives of this study were to identify the outcome of (LFTA). To identify the risk factors affecting development and severity of hepatotox...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.326.10725

    authors: Dizdar OS,Ersoy A,Aksoy S,Ozel Coskun BD,Yildiz A

    更新日期:2016-11-01 00:00:00

  • Body composition analysis to determine gender specific physical fitness equations in a cohort of Saudi population.

    abstract:OBJECTIVE:To determine an association between body composition analysis and physical fitness in the Saudi population and derive gender specific physical fitness equations. METHODS:A total of 530 healthy Saudi adults aged 15-72 years (mean 37.16±14.12 years) were enrolled in this study. Body composition analysis was as...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.304.4974

    authors: Iqbal M,Al-Regaiey KA,Ahmad S,Al Dokhi L,Al Naami M,Habib SS

    更新日期:2014-07-01 00:00:00

  • Labour induction with gestational hypertension: A great obstetric challenge.

    abstract:OBJECTIVE:To observe the fetomaternal morbidity and mortality with induction of labour in pregnant women with gestational hypertension. METHODS:The subjected study population included was 138 pregnant women with gestational hypertension. These pregnant women were between 34-40 weeks of gestational period in whom labou...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.331.10386

    authors: Khaskheli MN,Baloch S,Sheeba A,Baloch S,Khan F

    更新日期:2017-01-01 00:00:00

  • Clinical Audit of Dengue Related Deaths in 2011-Mayo Hospital Lahore Pakistan.

    abstract:Background and Objective:Dengue infection has evolved into an epidemic during last few years in Pakistan and has been associated with poor outcomes. Literature with respect to mortality risk factors in Dengue infection is not sufficient. This compelled us to conduct this study to find out major contributory factors to ...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.335.13051

    authors: Iqtadar S,Akbar N,Mehmood M,Abaidullah S

    更新日期:2017-09-01 00:00:00

  • Diagnostic accuracy of ultrasound in evaluation of obstructive jaundice with MRCP as gold standard.

    abstract:Objective:To evaluate the diagnostic accuracy of ultrasound in obstructive jaundice taking MRCP as gold standard. Methods:This cross-sectional study was conducted at Dow Institute of Radiology (DIR), Dow University of Health Sciences (DUHS), Karachi from 2nd May 2018 till 2nd November 2018. Both male and female patien...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.36.4.1665

    authors: Hanif H,Khan SA,Muneer S,Adil SO

    更新日期:2020-05-01 00:00:00

  • Effects of total thoracoscopic surgery on coronary artery fistulae complicated with ectasia.

    abstract:OBJECTIVE:To compare the effects of thoracotomy and total thoracoscopic surgery on coronary artery fistulae complicated with ectasia. METHODS:Forty-six patients with coronary artery fistulae complicated with ectasia were randomly divided into a treatment group and a control group (n=23) which were given totally thorac...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.321.7501

    authors: Sun H,Zhang L,Han X,Wang Z,Xu L

    更新日期:2016-01-01 00:00:00

  • Perceived stress and monetary burden among thalassemia patients and their caregivers.

    abstract:Objective:To find the perceived stress level and monetary burden in the thalassemia patients and their caregivers related to thalassemia treatment sessions. Methods:A descriptive cross sectional study was conducted at Rawalpindi Thalassemia Centre and Military Hospital Rawalpindi of six months' duration from November ...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.344.15420

    authors: Hisam A,Khan NUS,Tariq NA,Irfan H,Arif B,Noor M

    更新日期:2018-07-01 00:00:00

  • Alexithymia among elderly patients with diabetes.

    abstract:OBJECTIVE:To determine alexithymic characteristics and affecting factors in the elderly with diabetes mellitus. METHODS:This study was planned as a cross-sectional and descriptive study and was conducted in a state Hospital with 120 elderly patients with diabetes. Data were collected using a personal information form ...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:

    authors: Hintistan S,Cilingir D,Birinci N

    更新日期:2013-11-01 00:00:00

  • Effect of colporrhaphy on the Sexual Dysfunction of women with pelvic organ prolapsed.

    abstract:OBJECTIVE:To evaluate the effect of colporrhaphy on sexual problems of women with pelvic organ prolapseis. METHODOLOGY:Sixty patients with pelvic organ prolapse (cystocele - rectocele) in a quasi-experimental study before and after treatment were included. Presence of pelvic organ prolapseis was assessed by examinatio...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.291.2610

    authors: Shahghaibi S,Faizi S,Gharibi F

    更新日期:2013-01-01 00:00:00

  • Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients.

    abstract:Objective:To evaluate the association of miR-196a rs11614913 C/T genetic variation and its target gene annexin A1 mRNA expression with breast cancer risk in Pakistani female ethnicities. Methods:This case control study, conducted from March 2017 to November 2018 included 295 breast cancer patients, 295 controls of thr...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.35.6.1322

    authors: Rahim A,Afzal M,Naveed AK

    更新日期:2019-11-01 00:00:00

  • An assessment of ventilator-associated pneumonias and risk factors identified in the Intensive Care Unit.

    abstract:OBJECTIVES:Ventilator-associated pneumonia (VAP) is a significant cause of hospital-related infections, one that must be prevented due to its high morbidity and mortality. The purpose of this study was to evaluate the incidence and risk factors in patients developing VAP in our intensive care units (ICUs). METHODS:Thi...

    journal_title:Pakistan journal of medical sciences

    pub_type: 杂志文章

    doi:10.12669/pjms.324.10381

    authors: Karatas M,Saylan S,Kostakoglu U,Yilmaz G

    更新日期:2016-07-01 00:00:00