Abstract:
BACKGROUND AND OBJECTIVE:Breakpoint cluster region-Abelson (BCR-ABL) rearrangement or Philadelphia (Ph) chromosome in Chronic Myeloid Leukemia (CML) is derived from a reciprocal chromosomal translocation between ABL gene on chromosome 9 and BCR gene on chromosome 22. This chimeric protein has various sizes and therefore different clinical behaviour. The purpose of this study was to determine the heterogeneity of BCR-ABL rearrangement in patients with Ph(+)CML in Pakistan. METHODS:The study was conducted at Civil Hospital and Baqai Institute of Hematology (BIH) Karachi. Blood samples from 25 patients with CML were collected. Multiplex reverse transcription polymerase chain reaction (RT-PCR) was performed to identify various BCR-ABL transcripts. RESULTS:All 25 samples showed BCR-ABL rearrangements. Out of these, 24 (96%) patients expressed p210 BCR-ABL rearrangements i.e. 60% (n=15) had b3a2 and 32% (n=8) had b2a2 rearrangements. Co-expression of b3a2 /b2a2 rearrangement and p190 (e1a3) rearrangement was also identified in two patients. CONCLUSION:It is apparent that majority of the patients had p210 BCR-ABL rearrangements. Frequency of co-expression and rare fusion transcripts was very low.
journal_name
Pak J Med Scijournal_title
Pakistan journal of medical sciencesauthors
Tabassum N,Saboor M,Ghani R,Moinuddin Msubject
Has Abstractpub_date
2014-07-01 00:00:00pages
850-3issue
4eissn
1682-024Xissn
1681-715Xjournal_volume
30pub_type
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.293.2995
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abstract:Objective:To determine the frequency of Allergic Fungal Rhino-sinusitis (AFRS) in Chronic Rhino-sinusitis (CRS) patients and the accuracy of fungal culture in diagnosing AFRS. Methods:Immunocompetent patients with CRS and without invasive fungal rhino-sinusitis presenting over a period of 3 years in ENT department of ...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.3.1661
更新日期:2020-03-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
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pub_type: 杂志文章
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doi:10.12669/pjms.36.6.2325
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更新日期:2017-09-01 00:00:00
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更新日期:2016-01-01 00:00:00
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更新日期:2013-01-01 00:00:00
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更新日期:2016-07-01 00:00:00