TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells.

Abstract:

:Amelotin (AMTN) is induced upon initiation of apoptosis by transforming growth factor beta1 (TGFβ1) and is mediated by Smad3 in gingival epithelial cells (GE1 cells). This upregulation of AMTN gene expression is temporary, and the mechanism responsible is still unclear. The present study investigated the transcriptional downregulation of TGFβ1-induced AMTN gene expression in GE1 cells during the progression of apoptosis. To examine time-dependent changes in the levels of AMTN, Smad3 and Bax mRNA induced by TGFβ1, real-time PCR analyses were performed. Immunocytochemistry was carried out to detect the expression of Smad3 and Bax. Transient transfection analyses were performed using mouse AMTN gene promoter constructs of various lengths including Smad response elements (SBEs), in the presence or absence of TGFβ1. Changes in Smad3 binding to SBEs resulting from overexpression of Bax were examined using ChIP assays. Overexpression of Bax dramatically downregulated the levels of TGFβ1-induced AMTN mRNA and transcription of the AMTN gene. Smad3 binding to SBEs in the mouse AMTN gene promoter was induced by overexpression of Smad3 or TGFβ1, and this was inhibited by Bax overexpression. These results show that the levels of AMTN mRNA induced by TGFβ1 and Smad3 are decreased by robust expression of Bax in gingival epithelial cells.

journal_name

J Oral Sci

journal_title

Journal of oral science

authors

Nakayama Y,Matsui S,Noda K,Yamazaki M,Iwai Y,Ganss B,Ogata Y

doi

10.2334/josnusd.17-0271

subject

Has Abstract

pub_date

2018-06-20 00:00:00

pages

232-241

issue

2

eissn

1343-4934

issn

1880-4926

journal_volume

60

pub_type

杂志文章
  • Cellular hemangioma in an adult.

    abstract::This report describes an adult case of cellular hemangioma arising in the lower lip. A 39-year-old healthy woman presented with a polypoid mass of 4 months duration. The tumor imparted little color to the overlying mucosa and was misdiagnosed as a mucous granuloma preoperatively. The lobular proliferation of plump end...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.42.177

    authors: Shimoyama T,Horie N,Kato T,Kaneko T,Ide F

    更新日期:2000-09-01 00:00:00

  • Detection of mitochondrial DNA mutations in human gingival tissues.

    abstract::The accumulation of mitochondrial DNA mutation is an important contributor to the aging process. Polymerase chain reaction (PCR) amplification was carried out on total DNA from gingival tissues of human subjects with ages of 19 to 64 years, and 5.0-kb and 7.4-kb deletions were found in the mitochondrial genomes of the...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.42.221

    authors: Sugano N,Kawamoto K,Numazaki H,Murai S,Ito K

    更新日期:2000-12-01 00:00:00

  • Salivary alpha-amylase and lysozyme levels: a non-invasive technique for measuring parotid vs submandibular/sublingual gland activity.

    abstract::Cannulation procedures have shown that alpha-amylase is produced primarily by the parotid gland, whereas lysozyme is produced mainly by the submandibular and sublingual glands. In this study, the ratio of lysozyme to alpha-amylase was determined in whole human saliva following challenge with various gustatory and mech...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.42.83

    authors: Noble RE

    更新日期:2000-06-01 00:00:00

  • Effects of scanning technique on in vitro performance of CAD/CAM-fabricated fiber posts.

    abstract::This study assessed push-out strength, cement layer thickness, and interfacial nanoleakage of luted fiber posts fabricated with computer-aided design/computer-assisted manufacture (CAD/CAM) technology after use of 1 of 3 scanning techniques, namely, direct scanning of the post space (DS), scanning of a polyether impre...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.17-0254

    authors: Tsintsadze N,Juloski J,Carrabba M,Goracci C,Vichi A,Grandini S,Ferrari M

    更新日期:2018-01-01 00:00:00

  • Effects of chlorhexidine digluconate and hydrogen peroxide on Porphyromonas gingivalis hemin binding and coaggregation with oral streptococci.

    abstract::Porphyromonas gingivalis, a gram-negative anaerobe, is one of the major causative agents of periodontal disease. In this study, the effects of chlorhexidine digluconate and hydrogen peroxide on the hemin binding of P. gingivalis and coaggregation of this bacterium with oral streptococci were examined. The pretreatment...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.43.1

    authors: Lee SY

    更新日期:2001-03-01 00:00:00

  • Concentration of 137Cs in dried Lentinula edodes (Shiitake) as an indicator of environmental contamination.

    abstract::We analysed the concentration of radioactive cesium in dried Lentinula edodes (Shiitake) produced in Japan, and evaluated its use as an indicator of radioactive contamination. We collected Lentinula edodes (Shiitake) mushrooms grown on wood in 38 prefectures from north to south, and measured 137Cs, 134Cs and 40K conce...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.43.145

    authors: Shimizu M,Anzai I

    更新日期:2001-06-01 00:00:00

  • Oral surgery in patients with antiphospholipid syndrome.

    abstract::Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by recurrent thrombosis, other associated autoimmune disease, and/or obstetrical morbidity along with persistent production of antiphospholipid antibodies. Because of the nature of this systemic disease, most patients are medicated with an...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.18-0164

    authors: Okuyama K,Naruse T,Yutori H,Yanamoto S,Umeda M

    更新日期:2019-08-28 00:00:00

  • Gingival enlargement in children with sickle cell disease.

    abstract::The purpose of the present study was to evaluate periodontal health status in children with sickle cell disease (SCD). Forty-nine children with SCD and 39 systemically healthy sex- and age-matched children were enrolled in the study. Plaque index, gingival index, bleeding on probing, pocket depth, salivary volume, and...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.16-0796

    authors: Tonguç MÖ,Ünal S,Arpaci RB

    更新日期:2018-01-01 00:00:00

  • Biological plaque control: novel therapeutic approach to periodontal disease.

    abstract::Despite its important role in the control of periodontal disease, mechanical plaque control is not properly practiced by most individuals. Therefore, adjunctive chemical plaque control using chlorhexidine and antibiotics has also been suggested as an additional therapeutic strategy to augment mechanical plaque control...

    journal_title:Journal of oral science

    pub_type: 杂志文章,评审

    doi:10.2334/josnusd.54.1

    authors: Sugano N

    更新日期:2012-03-01 00:00:00

  • Role of HMGB1 in proliferation and migration of human gingival and periodontal ligament fibroblasts.

    abstract::High mobility group box 1 (HMGB1) was originally defined as a nuclear protein. However, later studies showed that HMGB1 was released from damaged cells into the extracellular milieu and functioned as a danger signaling molecule. HMGB1 has also been shown to exert proliferative and chemoattractant effects on many cell ...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.55.45

    authors: Chitanuwat A,Laosrisin N,Dhanesuan N

    更新日期:2013-03-01 00:00:00

  • The in vitro osteogenetic characteristics of primary osteoblastic cells from a rabbit calvarium.

    abstract::Previously, we showed that recombinant human bone morphogenetic protein-2 (rhBMP-2) increased bone augmentation beyond the skeletal envelope within a titanium cap in a rabbit calvarium; many cuboidal osteoblastic cells were observed histologically. These results suggested that the new osteoblastic cells might have dif...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.50.427

    authors: Hasegawa Y,Shimada K,Suzuki N,Takayama T,Kato T,Iizuka T,Sato S,Ito K

    更新日期:2008-12-01 00:00:00

  • Toll-like receptor 4 polymorphism is associated with increased susceptibility to chronic obstructive pulmonary disease in Han Chinese patients with chronic periodontitis.

    abstract::The aim of the present study was to evaluate the association of single nucleotide polymorphisms (SNPs) in toll-like receptor 4 (TLR4) with chronic obstructive pulmonary disease (COPD) in Han Chinese patients with chronic periodontitis (CP). Six candidate SNPs of TLR4-rs10759930, rs10983755, rs11536879, rs1927907, rs11...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.16-0187

    authors: Yu H,Lin M,Wang X,Wang S,Wang Z

    更新日期:2016-01-01 00:00:00

  • Dentist gender-related differences in patients' oral health behaviour.

    abstract::This study aimed at determining whether oral health behavior differs between patients regularly checked by male and female dentists. The analysis was based on a cross-sectional survey of 354 Japanese community residents (median age = 54 years; 145 men and 209 women) conducted in a municipality from January to February...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.18-0462

    authors: Takeuchi K,Noguchi Y,Nakai Y,Ojima T,Yamashita Y

    更新日期:2020-01-01 00:00:00

  • Bond strength to bovine dentin of a composite core build-up material combined with four different bonding agents.

    abstract::Clearfil DC Bond (DC) is a new single-step, dual-cure bonding agent. In this study, the shear bond strengths of a core build-up composite to dentin used with four bonding systems [DC, Unifil Core Self-Etching Bond (UC), Clearfil SE Bond (SE) and Cleafil tri-S Bond (TS)] were measured. The bonding ability after 7 days ...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.50.329

    authors: Ishii T,Ohara N,Oshima A,Koizumi H,Nakazawa M,Masuno T,Matsumura H

    更新日期:2008-09-01 00:00:00

  • Naso-oral fistula due to rhinolithiasis: a rare presentation.

    abstract::Naso-oral fistula, as the name suggests, is an abnormal communication between the nasal and oral cavities. It is an extremely rare clinical entity encountered in routine rhinology clinics across the world. The occurrence of this fistula due to rhinolithiasis is much rarer. We present one such case in a 40-year-old man...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.51.481

    authors: Arora S,Garg LN,Julaha M,Tuli BS

    更新日期:2009-09-01 00:00:00

  • Expression of connexins and the effect of retinoic acid in oral keratinocytes.

    abstract::Differential expression of members of the connexin (Cx) gap junction multigene family permits formation of gap junctions with the varied physiological properties required by different tissues. The aim of this study was to characterize connexin expression and the influence of all-trans-retinoic acid (RA) in mouse gingi...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.53.327

    authors: Hatakeyama S,Mikami T,Habano W,Takeda Y

    更新日期:2011-09-01 00:00:00

  • Endodontic treatment of a supernumerary tooth fused to a mandibular second molar: a case report.

    abstract::Since abnormal tooth morphology can predispose to caries and periodontal disease, careful management of fused teeth is essential. In this paper we report a rare case of a fused molar and supernumerary tooth and describe its management. Caries was removed from the tooth complex under local anesthesia. The pulp chamber ...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.48.39

    authors: Ghoddusi J,Zarei M,Jafarzadeh H

    更新日期:2006-03-01 00:00:00

  • The effects of low-level diode laser treatment and dental pulp-capping materials on the proliferation of L-929 fibroblasts.

    abstract::Low-level laser therapy (LLLT) has been reported to improve tissue healing and might therefore be useful in dental pulp capping after trauma. We evaluated the effects of a low-level diode laser (lambda = 680 nm) and dental pulp-capping substances on cell proliferation. Calcium hydroxide and adhesive resin were applied...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.52.33

    authors: Ferriello V,Faria MR,Cavalcanti BN

    更新日期:2010-03-01 00:00:00

  • Correlation of tooth mobility with systemic bone mineral density and periodontal status in Indian women.

    abstract::Imbalanced bone remodelling associated with osteopaenic or osteoporotic conditions can lead to a net bone loss throughout the skeleton, including the oral cavity, possibly leading to tooth mobility. This study investigated possible associations between systemic bone mineral density and both tooth mobility and periodon...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.54.177

    authors: Singh A,Sharma RK,Tewari S,Narula SC

    更新日期:2012-01-01 00:00:00

  • Tissue engineering in endodontics.

    abstract::Tissue engineering is the science of design and manufacture of new tissues to replace impaired or damaged ones. The key ingredients for tissue engineering are stem cells, the morphogens or growth factors that regulate their differentiation, and a scaffold of extracellular matrix that constitutes the microenvironment f...

    journal_title:Journal of oral science

    pub_type: 杂志文章,评审

    doi:10.2334/josnusd.51.495

    authors: Saber SE

    更新日期:2009-12-01 00:00:00

  • Asymptomatic oral human papillomavirus (HPV) infection in women with a histopathologic diagnosis of genital HPV.

    abstract::The oral route of human papillomavirus (HPV) transmission is not fully understood. It has been suggested that genital infection can act as a reservoir for oral HPV infection. We investigated the presence of oral HPV DNA and anti-HPV IgA in the buccal cavity of patients with a histopathologic diagnosis of cervical HPV ...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.53.451

    authors: Peixoto AP,Campos GS,Queiroz LB,Sardi SI

    更新日期:2011-12-01 00:00:00

  • Correlation of salivary and serum IgG, IgA levels with total protein in oral submucous fibrosis.

    abstract::Oral submucous fibrosis (OSMF) is a disabling, potentially malignant condition of the oral cavity. The aetiology of OSMF is multifactorial but remains obscure. Although arecanut is considered to be the most important causative agent, responses observed in individuals using arecanut vary in relation to quantity and dur...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.53.97

    authors: Patidar KA,Parwani RN,Wanjari SP

    更新日期:2011-03-01 00:00:00

  • The effect of mesenchymal stem cells on osteoclast precursor cell differentiation.

    abstract::Transplantation of mesenchymal stem cells (MSCs) has been extensively studied in the field of regenerative medicine. Bone regeneration is achieved via the interaction of osteoblasts and osteoclasts. However, the influence of MSCs on osteoclasts is unknown. The purpose of this study was to investigate the effect of MSC...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.17-0315

    authors: Abe T,Sumi K,Kunimatsu R,Oki N,Tsuka Y,Nakajima K,Ando K,Tanimoto K

    更新日期:2019-03-28 00:00:00

  • General anesthesia for a patient with deletion 6q syndrome in addition to laryngomalacia undergoing dental treatment.

    abstract::Chromosome 6 deletions are very rare (1,2), and deletion 6q syndrome is clinically characterized by mental and/or neuromotor retardation and microcephaly (3). Other alterations frequently observed are decreased biparietal diameter, hypertelorism, hypotelorism, absent eyebrows, prominent eyes with ptosis, receding chin...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.50.493

    authors: Fujii-Abe K,Sasao M,Fukayama H

    更新日期:2008-12-01 00:00:00

  • Fine needle aspiration biopsy (FNAB) in fibrous and non-fibrous soft oral tissue lesions.

    abstract::The use of fine needle aspiration biopsy (FNAB) in the diagnosis of many lesions in different parts of the body is quite widespread, while it has been used for oral lesions only very recently. This technique has many advantages including ease of use, cost effectiveness, convenience and accuracy. The present study, inv...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.41.41

    authors: Alatli C,Gürkan B,Koçak H,Ozveren A,Dölek S

    更新日期:1999-03-01 00:00:00

  • Cellular responses of periodontal ligament stem cells to a novel synthesized form of calcium hydrogen phosphate with a hydroxyapatite-like surface for periodontal tissue engineering.

    abstract::Calcium hydrogen phosphate with a hydroxyapatite-like surface (CHP-HA) is a novel synthesized compound designed to overcome the limitations of bioactive ceramics. It was originally applied as nano-sized HA strips covering core plates to enhance the degree of interfacial attachment. The objective of the present study w...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.17-0343

    authors: Tansriratanawong K,Wongwan P,Ishikawa H,Nakahara T,Wongravee K

    更新日期:2018-09-23 00:00:00

  • Compliance with supportive periodontal therapy among patients with aggressive and chronic periodontitis.

    abstract::Compliance with supportive periodontal therapy (SPT) depends on many factors but is generally poor. We compared SPT compliance among patients with aggressive and chronic periodontitis. This single-center longitudinal observational study enrolled 101 patients with generalized aggressive periodontitis (GAP; n = 52) or g...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.57.249

    authors: Agrawal N,Jain R,Jain M,Agarwal K,Dubey A

    更新日期:2015-09-01 00:00:00

  • Trends in domiciliary dental care including the need for oral appliances and dental technicians in Japan.

    abstract::The need for domiciliary dental care (DDC) for people requiring long-term nursing care is increasing as the super-aged society of Japan grows still older. Dysphagia diagnosis and rehabilitation are becoming more important in DDC; thus, the need for prostheses used for dysphasia rehabilitation is presumed to be increas...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.18-0053

    authors: Kamijo S,Sugimoto K,Oki M,Tsuchida Y,Suzuki T

    更新日期:2018-12-29 00:00:00

  • Mechanical effects of residual bone mass, a maxillofacial prosthesis, and a reconstruction plate on the mandible after marginal resection.

    abstract::In this study, analytic models were used to simulate marginal resection in the area of the second premolar to the second molar region, and the mechanical effects on the mandible of residual bone mass, a maxillofacial prosthesis, and a reconstruction plate were evaluated by three-dimensional finite element analysis. As...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.19-0427

    authors: Kase T,Ohyama T,Nakabayashi S,Yasuda H,Yoneyama T

    更新日期:2020-09-26 00:00:00

  • Evaluation of long-term satisfaction with orthodontic treatment for skeletal class III individuals.

    abstract::The aim of this study was to investigate the long-term satisfaction rate among skeletal class III patients following orthodontic treatment. Questionnaires were sent to 315 post-retention (between 5 to 22.5 years) patients who had received orthodontic treatment without any type of surgery for the correction of skeletal...

    journal_title:Journal of oral science

    pub_type: 杂志文章

    doi:10.2334/josnusd.49.31

    authors: Uslu O,Akcam MO

    更新日期:2007-03-01 00:00:00