Abstract:
:Culex mosquitoes can act as vectors of several important diseases, including Japanese encephalitis, West Nile virus, St. Louis encephalitis and equine encephalitis. Besides the neurological sequelae caused in humans, Japanese encephalitis can lead to abortion in sows and encephalitis in horses. Effective vector control and early diagnosis, along with continuous serosurveillance in animals, are crucial to fight this arboviral disease. However, the success of vector control operations is linked with the fast and reliable identification of targeted species, and knowledge about their biology and ecology. Since the DNA barcoding of Culex vectors of Japanese encephalitis is scarcely explored, here we evaluated the efficacy of this tool to identify and analyze the variations among five overlooked Culex vectors of Japanese encephalitis, Culex fuscocephala, Culex gelidus, Culex tritaeniorhynchus, Culex pseudovishnui and Culex vishnui, relying to the analysis of mitochondrial CO1 gene. Variations in their base pair range were elucidated by the entropy Hx plot. The differences among individual conspecifics and on base pair range across the same were studied. The C (501-750 bp) region showed a moderate variation among all the selected species. C. tritaeniorhynchus exhibited the highest variation in all the ranges. The observed genetic divergence was partially non-discriminatory. i.e., the overall intra- and inter nucleotide divergence was 0.0920 (0.92%) and 0.125 (1.25%), respectively. However, 10X rule fits accurately intraspecies divergence <3% for the five selected Culex species. The analysis of individual scatter plots showed threshold values (10X) of 0.008 (0.08%), 0.005 (0.05%), 0.123 (1.23%), 0.033 (0.33%) and 0.019 (0.19%) for C. fuscocephala, C. gelidus, C. tritaeniorhynchus, C. pseudovishnui and C. vishnui, respectively. The C. tritaeniorhynchus haplotypes KU497604, KU497603, AB690847 and AB690854 exhibited the highest divergence range, i.e., from 0.465 -0.546. Comparatively, the intra-divergence among the other haplotypes of C. tritaeniorhynchus ranged from 0-0.056. The maximum parsimony tree was formed by distinctive conspecific clusters with appreciable branch values illustrating their close congruence and extensive genetic deviations. Overall, this study adds valuable knowledge to the molecular biology and systematics of five overlooked mosquito species acting as major vectors of Japanese encephalitis in Asian countries.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Karthika P,Vadivalagan C,Thirumurugan D,Kumar RR,Murugan K,Canale A,Benelli Gdoi
10.1016/j.actatropica.2018.04.006subject
Has Abstractpub_date
2018-07-01 00:00:00pages
84-91eissn
0001-706Xissn
1873-6254pii
S0001-706X(18)30215-8journal_volume
183pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract::Malaria in pregnancy is associated with increased risks of maternal anemia, spontaneous abortion, low birth weight, premature delivery and other adverse effects on health. In Brazil, disease transmission is highly concentrated in the multi-state region that constitutes the Brazilian Amazon (more than 99% of all cases)...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2012.11.004
更新日期:2013-02-01 00:00:00
abstract::The trypanocidal activity of two commercially available isometamidium-based products, Samorin (Merial, USA) and Veridium (Sanofi Santé Nutrition Animale, France), used at a dose rate of 0.5 mg kg(-1) bodyweight, was compared in a field trial involving groups of approximately 30 zebu cattle in a trypanosomosis endemic ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/s0001-706x(00)00132-7
更新日期:2000-11-02 00:00:00
abstract::A high performance liquid chromatographic (HPLC) method for the determination of the antitheilerial drugs parvaquone and buparvaquone in plasma was developed. Both compounds were extracted from plasma with ether. After evaporating the extracts to dryness the residue was dissolved in methanol and an aliquot was injecte...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::Live bird market (LBM) is integral component in the perpetuation of HPAI H5N1, while biosecurity is crucial and key to the prevention and control of infectious diseases. Biosecurity compliance level and risk factor assessments in 155LBMs was evaluated in Nigeria and Egypt through the administration of a 68-item biosec...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2016.08.030
更新日期:2016-12-01 00:00:00
abstract::A cross-sectional sero-epidemiological study was conducted to determine the prevalence of dengue in Trinidad. Two commercial rapid test kits, PanBio Dengue Duo IgM and IgG Rapid Strip Test and the Bio-Check Plus Dengue G/M Cassette Test (Brittney) were used. The immunosorbent assay (ELISA) (FOCUS Technologies, Califor...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2006.11.009
更新日期:2007-02-01 00:00:00
abstract::Improving immunization coverage is vital to promoting child health and reducing childhood diseases and deaths. In spite of being actively promoted as a major public health intervention for national development since the late 1970s, immunization coverage in Ghana remains low. We investigated factors that influence atte...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/s0001-706x(97)00094-6
更新日期:1997-12-01 00:00:00
abstract::The semiargenteus-subgroup of the Tabanus bovinus species group is reviewed. The known distribution area of the three previously known species could be significantly increased. T. semiargenteus Olsufjev, 1937 was for the first time recorded from Syria and Iran, T. olsufjevi Hauser, 1960 from Iraq and Iran and T. sarba...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2014.04.027
更新日期:2014-09-01 00:00:00
abstract::> Qualitative research methods were used in rural and urban areas of the Greater Accra Region to generate data to describe the folk diagnosis, etiology and management of malaria. Respondents defined as fever a set of symptoms loosely concordant with clinical malaria. Primary cause of fever as heat and particularly in ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/0001-706x(94)90025-6
更新日期:1994-12-01 00:00:00
abstract::Macrophages infected with amastigotes of Leishmania mexicana mexicana as compared to normal macrophages show decreased migration both randomly and through a 5 microns pore in response to a known chemotaxin, an increased ability to pinocytose and an increased bactericidal ability. Unless very heavily parasitized their ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::Psychodopygus wellcomei (Diptera: Psychodidae), vector of Leishmania braziliensis braziliensis - causative agent of cutaneous leishmaniasis in Brazil, cannot be distinguished from sympatric P. complexus either by morphometrics or isoenzyme profiles. We report here the use of cuticular hydrocarbon analysis in the succe...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::This study identified the natural infection rate of Leishmania (Leishmania) infantum in Lutzomyia longipalpis sandflies collected in a neighborhood around a kennel, in Dracena, northwestern of São Paulo state. This region is highly endemic for visceral leishmaniasis in Brazil. Insects were captured during 2-3 nights m...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2018.01.012
更新日期:2018-05-01 00:00:00
abstract::Leishmaniasis is a protozoan infectious disease widely distributed all around the world. First line drugs including antimoniales are insufficient due to resistance in endemic areas and high toxicity. Azole antifungals like ketoconazole (KCZ) are also used as antileishmanial agents for several decades. In the present s...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2016.12.027
更新日期:2017-03-01 00:00:00
abstract::Visceral leishmaniasis is a life-threatening human disease commonly known as kala-azar. Leishmania donovani is the causative agent of this parasitic disease transmitted by the sand fly vector to infect hosts. Triphenyl tin salicylanilide thiosemicarbazone [Ph(3)Sn(OSal.TSCZH)] (TTST) which is an organometallic complex...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2005.03.008
更新日期:2005-07-01 00:00:00
abstract::The formation of Babesia ovis sporozoites in salivary gland cells of the vector tick Rhipicephalus bursa was studied by electron microscopy. The kinetics of B. ovis were found lying intracellularly on the second day after infestation (a.i.) of the ticks. The parasites enlarged rapidly losing all features of the motile...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::Leptospirosis is a zoonotic disease that is caused by pathogenic spirochaetes of Leptospira spp. and it has become a public health concern in urban localities in the tropics. Rats are important reservoir animals for the transmission of leptospirosis in urban areas. Leptospirosis is considered endemic in Vietnam. Howev...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2019.02.008
更新日期:2019-06-01 00:00:00
abstract::Smears of suspected patients infected with zoonotic cutaneous leishmaniasis (ZCL) were stained and examined under a light microscopic observation. DNA of parasites within human ulcers was extracted directly from their smears. Nested PCR was used to amplify a fragment containing the internal transcribed spacers of the ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2014.01.016
更新日期:2014-05-01 00:00:00
abstract::Field experiments were conducted in central Kenya, to study the effect of ammonium sulphate fertiliser ((NH(4))(2)SO(4)) on mosquito larval populations in rice fields. The experiments used a complete randomised block design having four blocks with two experimental ponds per block, and the fertiliser and control treatm...
journal_title:Acta tropica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.actatropica.2003.08.006
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Visceral leishmaniasis (VL) affecting HIV-infected patients is considered a challenging condition because of its high mortality and relapse rates. The approach of this condition is still surrounded by many uncertainties, especially regarding the criteria to institute and discontinue secondary prophylaxis for...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2017.05.011
更新日期:2017-08-01 00:00:00
abstract::Paragonimiasis is a subacute to chronic inflammatory granulomatous lung disease caused by the genus Paragonimus. In Latin America Paragonimus mexicanus Miyazaki & Ishii, 1968 is the only confirmed species to cause human infections. Paragonimus caliensis Little, 1968 is an uncommon species often regarded as a synonym o...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2018.03.024
更新日期:2018-07-01 00:00:00
abstract::Schistosomiasis is a snail-transmitted parasitic disease endemic in most rural areas of sub-Saharan Africa. However, the currently used prediction models fail to capture the focal nature of its transmission due to the macro-geographical levels considered and paucity of data at local levels. This study determined the s...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2016.03.028
更新日期:2016-07-01 00:00:00
abstract::In the present study for the first time an alternative definitive host model has been established for the complete development of Echinococcus granulosus in Meriones unguiculatus (Mongolian gerbils) up to shelled eggs production, as happened in the natural host. Animals treated with methylprednisolone acetate were ora...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2005.08.009
更新日期:2006-02-01 00:00:00
abstract::In Brazil, there are near 20 genera and almost 120 species of scorpions of which 95% reproduce sexually. Parthenogenetic reproduction, however, may also take place. To gain insight into useful molecular markers in parthenogenetic scorpion species, we studied DNA polymorphism using two molecular approaches: simple sequ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2009.07.002
更新日期:2009-11-01 00:00:00
abstract::Glutathione transferases (GSTs) are believed to be a major detoxification system in helminths. We describe the expression and functional analysis of EgGST, a cytosolic GST from Echinococcus granulosus, related to the Mu-class of mammalian enzymes. EgGST was produced as an enzymatically active dimeric protein (rEgGST),...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2009.12.003
更新日期:2010-04-01 00:00:00
abstract::Schistosomiasis remains a serious worldwide public health problem. Improving the diagnostic assay for surveillance and monitoring will contribute to hastening the possible elimination of the disease in endemic regions. Therefore, this study aims to develop magnetic affinity enzyme-linked immunoassay (MEIA) for serolog...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2012.08.006
更新日期:2012-12-01 00:00:00
abstract::Arboviruses have become a major public health concern in Brazil, especially after Zika virus (ZIKV) and Chikungunya virus (CHIKV) introduction, leading to massive epidemics. We conducted an investigation of arboviruses in patients with acute febrile illness for less than five days in Mato Grosso state (MT) during the ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2018.12.019
更新日期:2019-02-01 00:00:00
abstract::Human cytochrome P450 2C8 is a highly polymorphic gene and shows variation according to ethnicity. The CYP2C8*2 is a slow drug metabolism allele and shows 10-24% frequency in Black populations. The objective of this study was to assess the prevalence of CYP2C8*2 allele in Botswana among the San (or Bushmen) and the Ba...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2016.01.028
更新日期:2016-05-01 00:00:00
abstract::The parasitological techniques currently in use for the diagnosis of African trypanosomiasis were compared in a series of experiments for their capacity to detect Trypanosoma congolense, T. vivax and T. brucei in the blood of cattle. The darkground/phase contrast buffy coat method proved to be more sensitive than the ...
journal_title:Acta tropica
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::The present study performed an analysis of the ultrastructural changes induced by andiroba seed oil in the synganglion of Rhipicephalus sanguineus female ticks, aiming to provide scientific grounds to help in the creation of more specific and efficient methods of control. The synganglion consists of a mass of fused ne...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/j.actatropica.2014.06.018
更新日期:2015-01-01 00:00:00
abstract::The aetiology of zoonotic infections embraces organisms from every branch of the microbial world. In addition, zoonoses must be considered as a truly global problem, both in terms of their distribution and the measures required for their control. Within the UK, zoonotic disease is considered to be less frequent than i...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/s0001-706x(00)00093-0
更新日期:2000-07-21 00:00:00
abstract::In an endemic area for Wuchereria bancrofti filariasis in the Nile Delta, survival of adult female Culex pipiens was estimated by parity rate, mosquito infection and infectivity rates. Infection rates and 4th instar larval populations, as well as infection and parity rates, were linearly correlated. Infectivity correl...
journal_title:Acta tropica
pub_type: 杂志文章
doi:10.1016/0001-706x(89)90034-x
更新日期:1989-05-01 00:00:00