Abstract:
:We hypothesized that the P2X7 receptor may be the target of isoflurane, so we investigated the roles of the P2X7 receptor and inositol triphosphate receptor in calcium overload and neuronal apoptosis induced by isoflurane in cultured embryonic rat hippocampal neurons. Results showed that isoflurane induced widespread neuronal apoptosis and significantly increased cytoplasmic Ca(2+). Blockade of P2X7 receptors or removal of extracellular Ca(2+) combined with blockade of inositol triphosphate receptors completely inhibited apoptosis or increase in cytoplasmic Ca(2+). Removal of extracellular Ca(2+) or blockade of inositol triphosphate receptor alone could partly inhibit these effects of isoflurane. Isoflurane could directly activate P2X7-gated channels and induce inward currents, but did not affect the expression of P2X7 receptor protein in neurons. These findings indicate that the mechanism by which isoflurane induced neuronal apoptosis in rat developing brain was mediated by intracellular calcium overload, which was caused by P2X7 receptor mediated calcium influx and inositol triphosphate receptor mediated calcium release.
journal_name
Neural Regen Resjournal_title
Neural regeneration researchauthors
Liu H,Dai T,Guo Wdoi
10.3969/j.issn.1673-5374.2013.09.007subject
Has Abstractpub_date
2013-03-25 00:00:00pages
825-32issue
9eissn
1673-5374issn
1876-7958pii
NRR-8-825journal_volume
8pub_type
杂志文章abstract::As total life expectancy increases, the prevalence of age-related diseases such as diabetes and Alzheimer's disease is also increasing. Many hypotheses about Alzheimer's disease have been developed, including cholinergic neuron damage, oxidative stress, and inflammation. Acetylcholine is a major neurotransmitter in th...
journal_title:Neural regeneration research
pub_type: 杂志文章,评审
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journal_title:Neural regeneration research
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abstract::[This corrects the article DOI: 10.4103/1673-5374.245480]. ...
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pub_type: 杂志文章,已发布勘误
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journal_title:Neural regeneration research
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journal_title:Neural regeneration research
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pub_type: 杂志文章
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journal_title:Neural regeneration research
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journal_title:Neural regeneration research
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