Fusion protein of single-chain variable domain fragments for treatment of myasthenia gravis.

Abstract:

:Single-chain variable domain fragment (scFv) 637 is an antigen-specific scFv of myasthenia gravis. In this study, scFv and human serum albumin genes were conjugated and the fusion protein was expressed in Pichia pastoris. The affinity of scFv-human serum albumin fusion protein to bind to acetylcholine receptor at the neuromuscular junction of human intercostal muscles was detected by immunofluorescence staining. The ability of the fusion protein to block myasthenia gravis patient sera binding to acetylcholine receptors and its stability in healthy serum were measured by competitive ELISA. The results showed that the inhibition rate was 2.0-77.4%, and the stability of fusion protein in static healthy sera was about 3 days. This approach suggests the scFv-human serum albumin is a potential candidate for specific immunosuppressive therapy of myasthenia gravis.

journal_name

Neural Regen Res

authors

Li F,Meng F,Jin Q,Sun C,Li Y,Li H,Jin S

doi

10.4103/1673-5374.131611

subject

Has Abstract

pub_date

2014-04-15 00:00:00

pages

851-6

issue

8

eissn

1673-5374

issn

1876-7958

pii

NRR-9-851

journal_volume

9

pub_type

杂志文章
  • Rosmarinic acid elicits neuroprotection in ischemic stroke via Nrf2 and heme oxygenase 1 signaling.

    abstract::Rosmarinic acid (RA) can elicit a neuroprotective effect against ischemic stroke, but the precise molecular mechanism remains poorly understood. In this study, an experimental ischemic stroke model was established in CD-1 mice (Beijing Vital River Laboratory Animal Technology, Beijing, China) by occluding the right mi...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.241463

    authors: Cui HY,Zhang XJ,Yang Y,Zhang C,Zhu CH,Miao JY,Chen R

    更新日期:2018-12-01 00:00:00

  • Characteristics of mRNA dynamic expression related to spinal cord ischemia/reperfusion injury: a transcriptomics study.

    abstract::Following spinal cord ischemia/reperfusion injury, an endogenous damage system is immediately activated and participates in a cascade reaction. It is difficult to interpret dynamic changes in these pathways, but the examination of the transcriptome may provide some information. The transcriptome reflects highly dynami...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.179067

    authors: Qi ZP,Xia P,Hou TT,Li DY,Zheng CJ,Yang XY

    更新日期:2016-03-01 00:00:00

  • Does hemispheric lateralization influence therapeutic effects of transcranial direct current stimulation?

    abstract::This study investigated the effect of transcranial direct current stimulation (tDCS) polarity depending on lateralized function of task property in normal individuals performing visuomotor and simple repetitive tasks. Thirty healthy participants with no neurological disorders were recruited to participate in this stud...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.175057

    authors: Kwon YH,Kang KW,Lee NK,Son SM

    更新日期:2016-01-01 00:00:00

  • Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro.

    abstract::This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXC...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.133172

    authors: Chen X,Zhu Y,Han L,Lu H,Hao X,Dong Q

    更新日期:2014-05-15 00:00:00

  • Anomalous expression of chloride transporters in the sclerosed hippocampus of mesial temporal lobe epilepsy patients.

    abstract::The Na(+)-K(+)-Cl(-) cotransporter 1 and K(+)-Cl(-) cotransporter 2 regulate the levels of intracellular chloride in hippocampal cells. Impaired chloride transport by these proteins is thought to be involved in the pathophysiological mechanisms of mesial temporal lobe epilepsy. Imbalance in the relative expression of ...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.06.010

    authors: Cai X,Yang L,Zhou J,Zhu D,Guo Q,Chen Z,Chen S,Zhou L

    更新日期:2013-02-25 00:00:00

  • Modulation of mitochondrial bioenergetics as a therapeutic strategy in Alzheimer's disease.

    abstract::Alzheimer's disease (AD) is an increasingly pressing worldwide public-health, social, political and economic concern. Despite significant investment in multiple traditional therapeutic strategies that have achieved success in preclinical models addressing the pathological hallmarks of the disease, these efforts have n...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.224362

    authors: Onyango IG

    更新日期:2018-01-01 00:00:00

  • Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons.

    abstract::The iron chelator deferoxamine has been shown to inhibit ferroptosis in spinal cord injury. However, it is unclear whether deferoxamine directly protects neurons from ferroptotic cell death. By comparing the survival rate and morphology of primary neurons and SH-SY5Y cells exposed to erastin, it was found that these c...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.274344

    authors: Zhang Y,Fan BY,Pang YL,Shen WY,Wang X,Zhao CX,Li WX,Liu C,Kong XH,Ning GZ,Feng SQ,Yao X

    更新日期:2020-08-01 00:00:00

  • Inhibition of inflammatory mediator release from microglia can treat ischemic/hypoxic brain injury.

    abstract::Interleukin-1α and interleukin-1β aggravate neuronal injury by mediating the inflammatory reaction following ischemic/hypoxic brain injury. It remains unclear whether interleukin-1α and interleukin-1β are released by microglia or astrocytes. This study prepared hippocampal slices that were subsequently subjected to ox...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.13.001

    authors: Wang H,Guo W,Liu H,Zeng R,Lu M,Chen Z,Xiao Q

    更新日期:2013-05-05 00:00:00

  • Stem cell transplantation for spinal cord injury: a meta-analysis of treatment effectiveness and safety.

    abstract:OBJECTIVE:The aim of this study was to evaluate the effectiveness and safety of stem cell transplantation for spinal cord injury (SCI). DATA SOURCES:PubMed, EMBASE, Cochrane, China National Knowledge Infrastructure, China Science and Technology Journal, Wanfang, and SinoMed databases were systematically searched by co...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.206653

    authors: Fan X,Wang JZ,Lin XM,Zhang L

    更新日期:2017-05-01 00:00:00

  • Essential oils and functional herbs for healthy aging.

    abstract::As total life expectancy increases, the prevalence of age-related diseases such as diabetes and Alzheimer's disease is also increasing. Many hypotheses about Alzheimer's disease have been developed, including cholinergic neuron damage, oxidative stress, and inflammation. Acetylcholine is a major neurotransmitter in th...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.245467

    authors: Agatonovic-Kustrin S,Kustrin E,Morton DW

    更新日期:2019-03-01 00:00:00

  • Nerve growth factor in muscle afferent neurons of peripheral artery disease and autonomic function.

    abstract::In peripheral artery disease patients, the blood supply directed to the lower limbs is reduced. This results in severe limb ischemia and thereby enhances pain sensitivity in lower limbs. The painful perception is induced and exaggerate during walking, and is relieved by rest. This symptom is termed by intermittent cla...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.293132

    authors: Qin L,Li J

    更新日期:2021-04-01 00:00:00

  • Nuclear TAR DNA-binding protein 43: A new target for amyotrophic lateral sclerosis treatment.

    abstract::Abnormal TAR DNA-binding protein 43 (TDP-43) inclusion bodies can be detected in the degenerative neurons of amyotrophic lateral sclerosis. In this study, we induced chronic oxidative stress injury by applying malonate to cultured mouse cortical motor neurons. In the later stages of the malonate insult, TDP-43 express...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.35.003

    authors: Zheng M,Shi Y,Fan D

    更新日期:2013-12-15 00:00:00

  • Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha-synuclein.

    abstract::The specific and effective α-synuclein RNA interference (RNAi) plasmids, and the α-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 (HEK293) cells using the lipofectamine method. Using an inverted fluorescence microscope, α-synuclein proteins were observed to aggregate in the cy...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.02.001

    authors: Chen T,Liao X,Wen G,Deng Y,Guo M,Long Z,Ouyang F

    更新日期:2012-01-15 00:00:00

  • Six psychotropics for pre-symptomatic & early Alzheimer's (MCI), Parkinson's, and Huntington's disease modification.

    abstract::The quest for neuroprotective drugs to slow the progression of neurodegenerative diseases (NDDs), including Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD), has been largely unrewarding. Preclinical evidence suggests that repurposing quetiapine, lithium, valproate, fluoxetine, donepez...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.194708

    authors: Lauterbach EC

    更新日期:2016-11-01 00:00:00

  • Hydroxyethylstarch revisited for acute brain injury treatment.

    abstract::Infusion of the colloid hydroxyethylstarch has been used for volume substitution to maintain hemodynamics and microcirculation after e.g., severe blood loss. In the last decade it was revealed that hydroxyethylstarch can aggravate acute kidney injury, especially in septic patients. Because of the serious risk for crit...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.300978

    authors: Schick MA,Burek M,Förster CY,Nagai M,Wunder C,Neuhaus W

    更新日期:2021-07-01 00:00:00

  • Effects of fulvestrant on biological activity and Wnt expression in rat GH3 cells.

    abstract::The present study investigated the influence of anti-estrogen treatment (fulvestrant) on pituitary adenoma cell line GH3 biological activity, the estrogen receptor α pathway, the WnT pathway, and mechanisms of decreased Wnt inhibitory factor-1 expression in GH3 cells. Results showed that fulvestrant suppressed GH3 cel...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.04.008

    authors: Bai J,Wang Y,Li C,Zhang Y

    更新日期:2012-02-05 00:00:00

  • An efficient strategy for establishing a model of sensorineural deafness in rats.

    abstract::Ototoxic drugs can be used to produce a loss of cochlear hair cells to create animal models of deafness. However, to the best of our knowledge, there is no report on the establishment of a rat deafness model through the combined application of aminoglycosides and loop diuretics. The aim of this study was to use single...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.153704

    authors: Ma L,Yi HJ,Yuan FQ,Guo WW,Yang SM

    更新日期:2015-10-01 00:00:00

  • Phage display: development of nanocarriers for targeted drug delivery to the brain.

    abstract::The blood brain barrier represents a formidable obstacle for the transport of most systematically administered neurodiagnostics and neurotherapeutics to the brain. Phage display is a high throughput screening strategy that can be used for the construction of nanomaterial peptide libraries. These libraries can be scree...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.158330

    authors: Bakhshinejad B,Karimi M,Khalaj-Kondori M

    更新日期:2015-06-01 00:00:00

  • Neuroprotective effects of Buyang Huanwu decoction on cerebral ischemia-induced neuronal damage.

    abstract::Among the various treatment methods for stroke, increasing attention has been paid to traditional Chinese medicines. Buyang Huanwu decoction is a commonly used traditional Chinese medicine for the treatment of stroke. This paper summarizes the active components of the Chinese herb, which is composed of Huangqi (Radix ...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.141791

    authors: Mu Q,Liu P,Hu X,Gao H,Zheng X,Huang H

    更新日期:2014-09-01 00:00:00

  • Optimal concentration of necrostatin-1 for protecting against hippocampal neuronal damage in mice with status epilepticus.

    abstract::Hippocampal neurons undergo various forms of cell death after status epilepticus. Necrostatin-1 specifically inhibits necroptosis mediated by receptor interacting protein kinase 1 (RIP1) and RIP3 receptors. However, there are no reports of necroptosis in mouse models of status epilepticus. Therefore, in this study, we...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.268903

    authors: Lin DQ,Cai XY,Wang CH,Yang B,Liang RS

    更新日期:2020-05-01 00:00:00

  • PTEN inhibition and axon regeneration and neural repair.

    abstract::The intrinsic growth ability of all the neurons declines during development although some may grow better than others. Numerous intracellular signaling proteins and transcription factors have been shown to regulate the intrinsic growth capacity in mature neurons. Among them, PI3 kinase/Akt pathway is important for con...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.165496

    authors: Ohtake Y,Hayat U,Li S

    更新日期:2015-09-01 00:00:00

  • Putative adult neurogenesis in two domestic pigeon breeds (Columba livia domestica): racing homer versus utility carneau pigeons.

    abstract::Generation of neurons in the brains of adult birds has been studied extensively in the telencephalon of song birds and few studies are reported on the distribution of PCNA and DCX in the telencephalon of adult non-song learning birds. We report here on adult neurogenesis throughout the brains of two breeds of adult do...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.211187

    authors: Mazengenya P,Bhagwandin A,Nkomozepi P,Manger PR,Ihunwo AO

    更新日期:2017-07-01 00:00:00

  • Long non-coding RNA NONMMUG014387 promotes Schwann cell proliferation after peripheral nerve injury.

    abstract::Schwann cells play a critical role in peripheral nerve regeneration through dedifferentiation and proliferation. In a previous study, we performed microarray analysis of the sciatic nerve after injury. Accordingly, we predicted that long non-coding RNA NONMMUG014387 may promote Schwann cell proliferation after periphe...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.221168

    authors: Pan B,Shi ZJ,Yan JY,Li JH,Feng SQ

    更新日期:2017-12-01 00:00:00

  • Reversibility of visual field defects through induction of brain plasticity: vision restoration, recovery and rehabilitation using alternating current stimulation.

    abstract::For decades visual field defects were considered irreversible because it was thought that in the visual system the regeneration potential of the neuronal tissues is low. Nevertheless, there is always some potential for partial recovery of the visual field defect that can be achieved through induction of neuroplasticit...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.280302

    authors: Sabel BA,Gao Y,Antal A

    更新日期:2020-10-01 00:00:00

  • Nanoparticles carrying neurotrophin-3-modified Schwann cells promote repair of sciatic nerve defects.

    abstract::Schwann cells and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposome...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.14.002

    authors: Zong H,Zhao H,Zhao Y,Jia J,Yang L,Ma C,Zhang Y,Dong Y

    更新日期:2013-05-15 00:00:00

  • Effectiveness of lorazepam-assisted interviews in an adolescent with dissociative amnesia: A case report.

    abstract::To facilitate gathering information during a psychiatric interview, some psychiatrists advocate augmenting the interview using drugs. Rather than barbiturates, benzodiazepines have been used for drug-assisted interviews. Dissociative amnesia is one of the indications for these interviews. Herein, we present the case o...

    journal_title:Neural regeneration research

    pub_type:

    doi:10.3969/j.issn.1673-5374.2013.02.012

    authors: Seo Y,Shin MH,Kim SG,Kim JH

    更新日期:2013-01-15 00:00:00

  • Constraint-induced movement therapy promotes motor function recovery and downregulates phosphorylated extracellular regulated protein kinase expression in ischemic brain tissue of rats.

    abstract::Motor function impairment is a common outcome of stroke. Constraint-induced movement therapy (CIMT) involving intensive use of the impaired limb while restraining the unaffected limb is widely used to overcome the effects of 'learned non-use' and improve limb function after stroke. However, the underlying mechanism of...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.172319

    authors: Zhang B,He Q,Li YY,Li C,Bai YL,Hu YS,Zhang F

    更新日期:2015-12-01 00:00:00

  • Neuroprotective effects of statins against amyloid β-induced neurotoxicity.

    abstract::A growing body of evidence suggests that disruption of the homeostasis of lipid metabolism affects the pathogenesis of Alzheimer's disease (AD). In particular, dysregulation of cholesterol homeostasis in the brain has been reported to considerably increase the risk of developing AD. Thus, dysregulation of lipid homeos...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.226379

    authors: Li HH,Lin CL,Huang CN

    更新日期:2018-02-01 00:00:00

  • Paeonol attenuates inflammation-mediated neurotoxicity and microglial activation.

    abstract::Chronic activation of microglial cells endangers neuronal survival through the release of various proinflammatory and neurotoxic factors. The root of Paeonia lactiflora Pall has been considered useful for the treatment of various disorders in traditional oriental medicine. Paeonol, found in the root of Paeonia lactifl...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.18.001

    authors: Nam KN,Woo BC,Moon SK,Park SU,Park JY,Hwang JW,Bae HS,Ko CN,Lee EH

    更新日期:2013-06-25 00:00:00

  • Neuronal differentiation of adipose-derived stem cells and their transplantation for cerebral ischemia.

    abstract:OBJECTIVE:To review published data on the biological characteristics, differentiation and applications of adipose-derived stem cells in ischemic diseases. DATA RETRIEVAL:A computer-based online search of reports published from January 2005 to June 2012 related to the development of adipose-derived stem cells and their...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.25.011

    authors: Tian G,Zhou J,Wang J,Xu B,Li L,Zhu F,Han J,Li J,Zhang S,Luo X

    更新日期:2012-09-05 00:00:00