Abstract:
PURPOSE:Diseases that involve retinal or choroidal vascular endothelial cells are leading causes of vision loss: age-related macular degeneration, retinal ischemic vasculopathies, and noninfectious posterior uveitis. Proteins differentially expressed by these endothelial cell populations are potential drug targets. We used deep proteomic profiling to define the molecular phenotype of human retinal and choroidal endothelial cells at the protein level. METHODS:Retinal and choroidal vascular endothelial cells were separately isolated from 5 human eye pairs by selection on CD31. Total protein was extracted and digested, and peptide fractions were analyzed by reverse-phase liquid chromatography tandem mass spectrometry. Peptide sequences were assigned to fragment ion spectra, and proteins were inferred from openly accessible protein databases. Protein abundance was determined by spectral counting. Publicly available software packages were used to identify proteins that were differentially expressed between human retinal and choroidal endothelial cells, and to classify proteins that were highly abundant in each endothelial cell population. RESULTS:Human retinal and/or choroidal vascular endothelial cells expressed 5042 nonredundant proteins. Setting the differential expression false discovery rate at 0.05, 498 proteins of 3454 quantifiable proteins (14.4%) with minimum mean spectral counts of 2.5 were differentially abundant in the 2 cell populations. Retinal and choroidal endothelial cells were enriched in angiogenic proteins, and retinal endothelial cells were also enriched in immunologic proteins. CONCLUSIONS:This work describes the different protein expression profiles of human retinal and choroidal vascular endothelial cells, and provides multiple candidates for further study as novel treatments or drug targets for posterior eye diseases. NOTE: Publication of this article is sponsored by the American Ophthalmological Society.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Smith JR,David LL,Appukuttan B,Wilmarth PAdoi
10.1016/j.ajo.2018.03.020subject
Has Abstractpub_date
2018-09-01 00:00:00pages
197-229eissn
0002-9394issn
1879-1891pii
S0002-9394(18)30119-3journal_volume
193pub_type
杂志文章abstract::We used wide-field color specular microscopy of the human corneal surface to study the morphologic changes that occur under extended-wear contact lenses. There was a shift in the frequency distribution of surface cells by size to larger cells, suggesting a prolonged residence time on the ocular surface. Other abnormal...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(86)90818-4
更新日期:1986-03-15 00:00:00
abstract::We used 21 cadaver eyes to study transsclerally sutured, ciliary sulcus-fixated intraocular lens implantation. Results showed that transscleral sutures should exit the sclera less than 1 mm posterior to the corneoscleral limbus for true ciliary sulcus fixation. The relationship of the ciliary sulcus to the overlying p...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(89)90121-9
更新日期:1989-09-15 00:00:00
abstract:PURPOSE:To determine the safety and efficacy of verteporfin photodynamic therapy (PDT) for the treatment of persistent choroidal neovascularization (CNV) previously treated with external beam radiotherapy (EBRT). DESIGN:Retrospective case series. METHODS:Verteporfin PDT was performed on 14 eyes of 13 patients with sy...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2004.09.019
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:To determine whether the healing response of the human cornea after photoablation with a midinfrared solid-state laser was more pronounced than after excimer laser keratectomies because of the increased thermal damage in adjacent tissue. METHODS:We treated five blind human eyes with an erbium:YAG (Er:YAG) lase...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)72215-9
更新日期:1995-11-01 00:00:00
abstract::We used a handheld disposable cautery for the diagnostic scraping of corneal ulcers. Sterility of the tip is obtained by activating the unit until the tip reaches a red-hot color and then allowing it to cool. The unit has a sturdy, well-defined tip, and is self-sterilizing, reusable, readily available, and simple to u...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)75086-x
更新日期:1980-07-01 00:00:00
abstract:PURPOSE:Descemet membrane endothelial keratoplasty is a new technique for the replacement of diseased corneal endothelium with healthy donor endothelium. During this procedure, manipulation of the donor endothelium-Descemet membrane layer within the recipient anterior chamber can be associated with loss of the correct ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2010.01.005
更新日期:2010-06-01 00:00:00
abstract:PURPOSE:To determine the morphologic changes in human corneas over time following radiofrequency-based conductive keratoplasty (CK) treatment. DESIGN:Prospective, observational case series. METHODS:In a single-center study six human corneas of six patients with localized peripheral keratoconus underwent CK treatment ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2005.06.027
更新日期:2005-12-01 00:00:00
abstract:PURPOSE:To describe the long-term surgical course of patients with open globe injury. DESIGN:Retrospective case series. METHODS:Patients with open globe injuries (848 in total) treated surgically at the Massachusetts Eye and Ear Infirmary between 2000 and 2009 were retrospectively reviewed. Data from presentation, in...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2011.10.013
更新日期:2012-05-01 00:00:00
abstract:PURPOSE:To evaluate the complications associated with the primary placement of a motility coupling post (MCP) in spherical porous polyethylene (PP) implants at the time of enucleation. DESIGN:Retrospective, interventional case series. METHODS:The records of all patients who had undergone primary enucleation and spher...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2007.01.049
更新日期:2007-05-01 00:00:00
abstract:PURPOSE:To determine if adequate intraocular levels of methotrexate are achieved after intravenous administration. METHODS:After intravenous administration, methotrexate levels were determined in the serum, the anterior chamber, and the cerebrospinal fluids of a patient with recurrent ocular lymphoma. A fluorescence p...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)70444-1
更新日期:1996-04-01 00:00:00
abstract::In an 8-year-old girl with asymptomatic proptosis, computed tomographic scans showed a large medial orbital mass that contoured the globe anteriorly, bowed the optic nerve laterally, and extended posteriorly to the orbital apex. T1-weighted coronal magnetic resonance images showed the mass to be a diffusely enlarged m...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)77878-x
更新日期:1990-11-15 00:00:00
abstract:PURPOSE:Epiretinal membranes (ERMs) in highly myopic eyes may result in macular holes and subsequent retinal detachment. However, removing friable, thin ERMs from detached retinas is often difficult. We report the efficacy of a diamond-dusted silicone cannula in the removal of ERMs from detached retinas. METHODS:Seven...
journal_title:American journal of ophthalmology
pub_type: 临床试验,杂志文章
doi:10.1016/s0002-9394(98)00180-9
更新日期:1998-11-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the baseline features associated with blindness in a cohort of children with primary congenital glaucoma (PCG) from a hospital registry. METHODS:Setting: Observational cohort study DESIGN: Retrospective clinical cohort study STUDY POPULATION: The registry included all chil...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2020.12.014
更新日期:2020-12-21 00:00:00
abstract:PURPOSE:To use the capillary-free zone along retinal arteries, a physiologic area of superficial avascularization, as an anatomic paradigm to investigate the reliability of optical coherence tomography angiography (OCTA) for visualizing the deep retinal circulation. DESIGN:Validity analysis and laboratory investigatio...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2018.02.007
更新日期:2018-05-01 00:00:00
abstract:PURPOSE:To report the clinical outcomes of penetrating keratoplasty (PK) after autologous cultivated limbal epithelial transplantation in eyes with limbal stem cell deficiency (LSCD) after ocular surface burns. DESIGN:Retrospective case series. METHODS:This study included 47 patients with unilateral LSCD treated by a...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2011.05.019
更新日期:2011-12-01 00:00:00
abstract:PURPOSE:To evaluate the co-occurrence of acute ischemic stroke and acute retinal artery occlusion (RAO). DESIGN:Retrospective observational case series. METHODS:We included 33 consecutive patients with acute RAO who underwent diffusion-weighted magnetic resonance imaging within 7 days of the onset of visual symptoms....
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2014.01.033
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:To document and study the cross-sectional structures of polypoidal elements using optical coherence tomography in eyes with idiopathic polypoidal choroidal vasculopathy. METHODS:Optical coherence tomography images of two eyes with idiopathic polypoidal choroidal vasculopathy were correlated with slit-lamp biom...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(98)00411-5
更新日期:1999-03-01 00:00:00
abstract::The diameter of retinal arterioles, venules, and their macular branches was measured before and after macular laser photocoagulation in one eye each of six men and eight women with diabetic macular edema. The macular arteriolar branches constricted 20.2% (P < .001) and the venular branches constricted 13.8% (P < .001)...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)73526-3
更新日期:1993-01-01 00:00:00
abstract::A 70-year-old man had unilateral congestion of the right upper eyelid, which contained a nodular mass. A biopsy was performed, and histologic, immunocytochemical, and ultrastructural studies disclosed a pseudoneoplastic proliferation of endothelial cells and pericytes in a region containing clumps of bacteria. This co...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)72894-6
更新日期:1994-08-15 00:00:00
abstract::We used a technique for surgical management of fibrovascular membranes to relieve epiretinal tangential traction when present in proliferative diabetic retinopathy. Sharp dissection with intraocular scissors was used to segment fibrovascular and cortical vitreous membranes together with cauterization of large vessels ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(80)90012-4
更新日期:1980-03-01 00:00:00
abstract::Six patients (five men and one woman, 19 to 57 years old) with laboratory-proven fungal corneal infections were successfully treated with topical ketoconazole, a synthetic imidazole derivative. No signs of progression of the corneal infection were seen after the antifungal therapy was initiated. The clinical signs of ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(85)90795-0
更新日期:1985-08-15 00:00:00
abstract::We re-assessed nine patients (aged 2 to 28 years) in whom true Brown's syndrome had been diagnosed and superior oblique muscle tenotomy performed. The postoperative period ranged from three to 12 years. The final results were divided into three groups: (1) Patients with partial residual Brown's syndrome postoperativel...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(80)90173-7
更新日期:1980-06-01 00:00:00
abstract::Of nine patients (five men and four women, 25 to 55 years old) with localized orbital neurofibromas, only one had other systemic findings consistent with neurofibromatosis. The neurofibromas originated from sensory nerves of the orbit, producing gradual proptosis with expansion of the orbital walls in most cases. Mild...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(85)90514-8
更新日期:1985-09-15 00:00:00
abstract::Standard retinal function tests are of limited value in assessing retinal function in eyes with opaque vitreous. We developed a method for obtaining an electroretinogram (ERG) from eyes with significant vitreous opacity by utilizing a much brighter than normal stimulating light. Of 115 eyes with vitreous opacities and...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(75)90135-x
更新日期:1975-08-01 00:00:00
abstract:PURPOSE:To describe two cases of acute traumatic maculopathy using optical coherence tomography (OCT) following high-speed motor vehicle accidents. DESIGN:Case reports. METHODS:Patients were examined soon after the accident using Snellen visual acuity (VA), dilated fundus examination, and OCT. Re-examination was cond...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2006.09.024
更新日期:2007-02-01 00:00:00
abstract:PURPOSE:To evaluate the effects of the Boston Ocular Surface Prosthesis (Boston Foundation for Sight, Needham, Massachusetts, USA) on visual acuity (VA) and visual functioning in patients with severe corneal ectasia, irregular astigmatism, or ocular surface disease. DESIGN:Prospective, interventional case series. MET...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2009.07.037
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:To report the efficacy and safety of outpatient fluid-gas exchange for open macular hole after primary vitrectomy. DESIGN:Retrospective interventional case series. METHODS:setting: Institutional. patients: Thirty-six patients with primary failed closure or reopened holes after primary vitrectomy. intervention...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2013.03.031
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:To report on a case of acute transient myopia associated with ciliochoroidal detachment induced by indapamide. METHOD:Case report. Clinical examination, ultrasonography, and fluorescein angiography were performed during the acute phase of disease and convalescence. RESULTS:After indapamide was discontinued, a...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(99)00402-x
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:To assess the efficacy of intravitreous fomivirsen sodium, an antisense oligonucleotide, for newly diagnosed peripheral cytomegalovirus (CMV) retinitis in patients with acquired immunodeficiency syndrome (AIDS). DESIGN:Randomized treatment intervention clinical trial. METHODS:A multicenter, prospective, and r...
journal_title:American journal of ophthalmology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s0002-9394(02)01327-2
更新日期:2002-04-01 00:00:00
abstract:PURPOSE:To assess the optic nerve head (ONH) by optical coherence tomography (OCT), confocal scanning laser ophthalmoscopy (CSLO), and the retinal nerve fiber layer (RNFL) by OCT and scanning laser polarimetry (GDx) in highly myopic subjects. DESIGN:Observational cross-sectional study. METHODS:Thirty-five eyes of hig...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2006.05.022
更新日期:2006-11-01 00:00:00