Abstract:
:We investigated the endogenous mono(ADP-ribosyl)ation of the sarcoplasmic reticulum from rabbit skeletal muscle. The autoradiogram obtained after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the [adenylate-32P]NAD-treated sarcoplasmic reticulum vesicles revealed a major band corresponding to the MW 105 K Ca2+-dependent ATPase and other bands corresponding to proteins of MW 153, 60 and 38 K and those of 125 to 135 K range. The addition of poly L-lysine during the incubation led to an enhancement of the modification. Poly L-lysine is proving to be a pertinent tool for identifying acceptor proteins.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Hara N,Mishima K,Tsuchiya M,Tanigawa Y,Shimoyama Mdoi
10.1016/s0006-291x(87)80043-8subject
Has Abstractpub_date
1987-04-29 00:00:00pages
856-62issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(87)80043-8journal_volume
144pub_type
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