Mice lacking Axl and Mer tyrosine kinase receptors are susceptible to experimental autoimmune orchitis induction.

Abstract:

:The mammalian testis is an immunoprivileged organ where male germ cell autoantigens are immunologically ignored. Both systemic immune tolerance to autoantigens and local immunosuppressive milieu contribute to the testicular immune privilege. Testicular immunosuppression has been intensively studied, but information on systemic immune tolerance to autoantigens is lacking. In the present study, we aimed to determine the role of Axl and Mer receptor tyrosine kinases in maintaining the systemic tolerance to male germ cell antigens using the experimental autoimmune orchitis (EAO) model. Axl and Mer double-knockout (Axl(-/-)Mer(-/-)) mice developed evident EAO after a single immunization with germ cell homogenates emulsified with complete Freund's adjuvant. EAO was characterized by the accumulation of macrophages and T lymphocytes in the testis. Damage to the seminiferous epithelium was also observed. EAO induction was associated with pro-inflammatory cytokine upregulation in the testes, impaired permeability of the blood-testis barrier and generation of autoantibodies against germ cell antigens in Axl(-/-)Mer(-/-) mice. Immunization also induced mild EAO in Axl or Mer single-gene-knockout mice. By contrast, a single immunization failed to induce EAO in wild-type mice. The results indicate that Axl and Mer receptors cooperatively regulate the systemic immune tolerance to male germ cell antigens.

journal_name

Immunol Cell Biol

authors

Li N,Liu Z,Zhang Y,Chen Q,Liu P,Cheng CY,Lee WM,Chen Y,Han D

doi

10.1038/icb.2014.97

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

311-20

issue

3

eissn

0818-9641

issn

1440-1711

pii

icb201497

journal_volume

93

pub_type

杂志文章
  • The MS4A family: counting past 1, 2 and 3.

    abstract::The MS4A (membrane-spanning 4-domain family, subfamily A) family of proteins contains some well-known members including MS4A1 (CD20), MS4A2 (FcɛRIβ) and MS4A3 (HTm4). These three MS4A family members are expressed on the cell surface of specific leukocyte subsets and have been well characterized as having key roles in ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2015.48

    authors: Eon Kuek L,Leffler M,Mackay GA,Hulett MD

    更新日期:2016-01-01 00:00:00

  • Does recycling in germinal centres exist?

    abstract::A general criterion is formulated to decide if the recycling of B cells exists in germinal centre (GC) reactions. The criterion is independent of the selection and affinity maturation process and based solely on total centroblast population arguments. An experimental test is proposed to verify whether the criterion is...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1711.2002.01052.x

    authors: Meyer-Hermann M

    更新日期:2002-02-01 00:00:00

  • c-Cbl: a regulator of T cell receptor-mediated signalling.

    abstract::The 120-kDa protein product of the c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic protein that is especially abundant in the thymus, indicating an important role for Cbl in thymic signalling. c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain, a C3HC4 RING finger motif, multiple...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.1998.00768.x

    authors: Thien CB,Langdon WY

    更新日期:1998-10-01 00:00:00

  • Recombinant vaccines against ovine footrot.

    abstract::For the past 20 years footrot vaccines have evolved from simple bacterins to highly specific recombinant DNA (rDNA) fimbrial vaccines. The development of these vaccines has left a trail of discoveries, challenges and solutions; these processes continue as we move closer to understanding the requirements of a footrot v...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.1993.53

    authors: O'Meara TJ,Egerton JR,Raadsma HW

    更新日期:1993-10-01 00:00:00

  • Listeria species escape from the phagosomes of interleukin-4-deactivated human macrophages independent of listeriolysin.

    abstract::Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1711.2003.01196.x

    authors: Neumann K,Eppler E,Filgueira L,Groscurth P,Gasal E,Schaffner A,Schoedon G,Schneemann M

    更新日期:2003-12-01 00:00:00

  • Expression of the T cell receptor delta-chain repertoire in mouse lymph node.

    abstract::Despite the potential for extensive diversity of gamma delta TCR, especially for delta-chains, expression of the gamma delta TCR repertoire in various mouse epithelial tissues is highly restricted. This implies that the recognition of antigen by gamma delta T cells may also be limited. To date, however, few studies ha...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1996.56

    authors: Olive C

    更新日期:1996-08-01 00:00:00

  • Transgenic plants for the production of veterinary vaccines.

    abstract::The expression of antigens in transgenic plants has been increasingly used in the development of experimental vaccines, particularly oriented to the development of edible vaccines. Hence, this technology becomes highly suitable to express immunogenic proteins from pathogens. Foot and mouth disease virus, bovine rotavi...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1711.2005.01338.x

    authors: Dus Santos MJ,Wigdorovitz A

    更新日期:2005-06-01 00:00:00

  • Elevated granzyme B+ B-cell level in SIV-infection correlate with viral load and low CD4 T-cell count.

    abstract::Granzyme B-expressing (GrB+) B cells are thought to contribute to immune dysfunctions in HIV patients, but so far their exact role is unknown. This report demonstrates for the first time the existence of GrB+ B cells in SIV-infected rhesus macaques, which represent the most commonly used nonhuman primate model for HIV...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2016.96

    authors: Kotb A,Klippert A,Daskalaki M,Sauermann U,Stahl-Hennig C,Neumann B

    更新日期:2017-03-01 00:00:00

  • A probabilistic view of immunology: drawing parallels with physics.

    abstract::Regulating the strength and class of an immune response requires lymphocytes to act as complex signal integrating "machines", taking information from multiple sources while making decisions that affect the final outcome. Describing and understanding the decision-making behaviour of lymphocytes within the context of th...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/sj.icb.7100061

    authors: Hodgkin PD

    更新日期:2007-06-01 00:00:00

  • Characterization of a bioassay for detection of recombinant and native ovine interleukin-5.

    abstract::In order to analyse Th2-type immune responses in sheep by the assay of interleukin (IL)-5 in biological fluids, the ovine IL-5 gene was cloned and expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus expression vector system. The recombinant product was purified as BAC-OV-IL-5 from the supernatant...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1711.1999.00831.x

    authors: Bosward K,Emery DL,McWaters PW,Husband AJ,Bendixsen T

    更新日期:1999-08-01 00:00:00

  • Quantifying complement-mediated phagocytosis by human monocyte-derived macrophages.

    abstract::The present study demonstrates that SRBC can be opsonized with untreated human serum such that lysis by active complement components is minimal but sufficient opsonization occurs to permit high rates of complement-mediated phagocytosis. Phagocytosis of SRBC opsonized with 2% whole human serum by human monocyte-derived...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1711.2001.01027.x

    authors: Chan HT,Kedzierska K,O'Mullane J,Crowe SM,Jaworowski A

    更新日期:2001-10-01 00:00:00

  • Many human immunoglobulin heavy-chain IGHV gene polymorphisms have been reported in error.

    abstract::The identification of the genes that make up rearranged immunoglobulin genes is critical to many studies. For example, the enumeration of mutations in immunoglobulin genes is important for the prognosis of chronic lymphocytic leukemia, and this requires the accurate identification of the germline genes from which a pa...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/sj.icb.7100144

    authors: Wang Y,Jackson KJ,Sewell WA,Collins AM

    更新日期:2008-02-01 00:00:00

  • Leukaemogenic progression: the importance of differentiation and associated genetic events.

    abstract::A feature common to many blood cell cancers is the uncoupling of normal proliferative and differentiative events, both of which are intimately linked in the cell's developmental programme. In some cancers, further differentiative events have been associated with oncogenic progression and, in other cancers, terminal di...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.1995.32

    authors: Ho ES,O'Neill HC

    更新日期:1995-06-01 00:00:00

  • Regulating the balance between necroptosis, apoptosis and inflammation by inhibitors of apoptosis proteins.

    abstract::Understanding how inhibitor of apoptosis proteins (IAPs) regulate apoptosis and necroptosis has been fast-forwarded by the use of Smac mimetics (SMs) to deplete or inhibit the IAPs, specifically cIAP1, cIAP2 and XIAP. The loss or inhibition of cIAP1, cIAP2 and XIAP causes the majority of cells to be sensitized to deat...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2016.118

    authors: Vasilikos L,Spilgies LM,Knop J,Wong WW

    更新日期:2017-02-01 00:00:00

  • Tumour necrosis factor and lymphotoxin: molecular aspects and role in tissue-specific autoimmunity.

    abstract::Tumour necrosis factor (TNF) is a highly potent, proinflammatory cytokine with broad-ranging functions from the regulation of endothelial cell adhesion molecules to facilitate entry of leucocytes into tissues, to direct induction of cellular cytotoxicity. This diversity of function potentially attributable to TNF in t...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.1996.77

    authors: Körner H,Sedgwick JD

    更新日期:1996-10-01 00:00:00

  • Effects of IL-10- and FasL-overexpressing dendritic cells on liver transplantation tolerance in a heterotopic liver transplantation rat model.

    abstract::Acute rejection is the major determinant for the long-term survival of donor liver after liver transplantation (LT). The aim of this study was to examine the therapeutic potential of interleukin (IL)-10-FasL-overexpressing immature dendritic cells (imDCs) to induce local immunosuppression in liver grafts. imDCs derive...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1111/imcb.12252

    authors: Chen L,Zhang L,Zhu Z,He W,Gao L,Zhang W,Liu J,Huang A

    更新日期:2019-09-01 00:00:00

  • Purity of transferred CD8(+) T cells is crucial for safety and efficacy of combinatorial tumor immunotherapy in the absence of SHP-1.

    abstract::Adoptive transfer of tumor-specific cytotoxic T cells is a promising advance in cancer therapy. Similarly, checkpoint inhibition has shown striking clinical results in some patients. Here we combine adoptive cell transfer with ablation of the checkpoint protein Src homology 2-domain-containing phosphatase 1 (SHP-1, Pt...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2016.45

    authors: Watson HA,Dolton G,Ohme J,Ladell K,Vigar M,Wehenkel S,Hindley J,Mohammed RN,Miners K,Luckwell RA,Price DA,Matthews RJ,Ager A

    更新日期:2016-09-01 00:00:00

  • Single-cell technologies are revolutionizing the approach to rare cells.

    abstract::In the last lustrum single-cell techniques such as single-cell quantitative PCR, RNA and DNA sequencing, and the state-of-the-art cytometry by time of flight (CyTOF) mass cytometer have allowed a detailed analysis of the sub-composition of different organs from the bone marrow hematopoietic compartment to the brain. T...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2015.106

    authors: Proserpio V,Lönnberg T

    更新日期:2016-03-01 00:00:00

  • Relevance of necroptosis in cancer.

    abstract::Resistance to caspase-dependent apoptosis is often responsible for treatment failures in cancer. Finding novel therapeutic strategies that can activate alternative cell death programs appears to be appealing. Necroptosis is a form of programmed necrosis that occurs under caspase-deficient conditions. This alternative ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2016.120

    authors: Lalaoui N,Brumatti G

    更新日期:2017-02-01 00:00:00

  • B cells: we need them now more than ever.

    abstract::The humoral immune response, that is, the production of antibodies by B cells, is a critical component of immunity to infection and underlies the protection provided by the majority of successful vaccines. This Special Feature explores some of the latest advances in understanding B cell activation and differentiation,...

    journal_title:Immunology and cell biology

    pub_type: 社论

    doi:10.1111/imcb.12348

    authors: Deenick EK

    更新日期:2020-07-01 00:00:00

  • Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens.

    abstract::Antibodies can have a protective but non-essential role in natural chlamydial infections dependent on antigen specificity and antibody isotype. IgG is the dominant antibody in both male and female reproductive tract mucosal secretions, and is bi-directionally trafficked across epithelia by the neonatal Fc receptor (Fc...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2013.110

    authors: Armitage CW,O'Meara CP,Harvie MC,Timms P,Blumberg RS,Beagley KW

    更新日期:2014-05-01 00:00:00

  • Mechanisms involved in the inhibition of growth of a human B lymphoma cell line, B104, by anti-MHC class II antibodies.

    abstract::The mechanisms involved in the inhibition of growth of a human B lymphoma cell line, B104, by anti-MHC class II antibodies (Ab) were compared with those in anti-IgM Ab-induced B104 growth inhibition. Two anti-MHC class II Ab, L227 and 2.06, inhibited the growth of B104 cells, although 2.06, but not L227, needed to be ...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1994.31

    authors: Higaki Y,Hata D,Kanazashi S,Horiguchi Y,Yamaoka K,Ohshima Y,Kim KM,Heike T,Mayumi M

    更新日期:1994-06-01 00:00:00

  • 3D computation modelling of the influence of cytokine secretion on Th-cell development suggests that negative selection (inhibition of Th1 cells) is more effective than positive selection by IL-4 for Th2 cell dominance.

    abstract::Th-cell development has been suggested to include selective mechanisms in which certain cytokines select either Th1 or Th2 cells to proliferate and grow. The selective theory is based on the observation that Th2 cells secrete IL-4, a cytokine that promotes Th2 development, whereas Th1 cells secrete interferon-gamma (I...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/sj.icb.7100023

    authors: Jansson A,Harlen M,Karlsson S,Nilsson P,Cooley M

    更新日期:2007-04-01 00:00:00

  • Lymphopenic effects on mice of a component of ammonia caramel, 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI).

    abstract::A derivative of ammonia caramel colour (AC) is known to induce a selective lymphopenia in rats. Accordingly, the haematological effects were studied in mice of oral administration in drinking water of 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI), the component of AC responsible for lymphopenia. Initially five groups...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.1988.51

    authors: Iscaro A,Mackay IR,O'Brien C

    更新日期:1988-10-01 00:00:00

  • Autophagy as a pro-death pathway.

    abstract::The evolutionarily conserved catabolic process of autophagy involves the degradation of cytoplasmic components through lysosomal enzymes. Basal levels of autophagy maintain cellular homeostasis and under stress conditions high levels of autophagy are induced. It is often under such stress conditions that high levels o...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/icb.2014.85

    authors: Denton D,Xu T,Kumar S

    更新日期:2015-01-01 00:00:00

  • Immunoepigenetics: the unseen side of cancer immunoediting.

    abstract::Cancer immunosurveillance representing, till recently, the explanatory framework relating cancer and the immune system, does not convincingly explain tumor escape. At the beginning of the decade, a new theory emerged, namely the immunoediting theory, and it comprehensively defines the role of the immune system in carc...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章,评审

    doi:10.1038/sj.icb.7100006

    authors: Germenis AE,Karanikas V

    更新日期:2007-01-01 00:00:00

  • B and T lymphocyte attenuator interacts with CD3zeta and inhibits tyrosine phosphorylation of TCRzeta complex during T-cell activation.

    abstract::B and T lymphocyte attenuator (BTLA) is an important negative regulator of T-cell activation. T-cell activation involves partitioning of receptors into discrete membrane compartments known as lipid rafts and the formation of an immunological synapse (IS) between the T cell and antigen-presenting cell (APC). Here we sh...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/sj.icb.7100087

    authors: Wu TH,Zhen Y,Zeng C,Yi HF,Zhao Y

    更新日期:2007-11-01 00:00:00

  • Plasmacytoid and conventional dendritic cells are early producers of IL-12 in Neospora caninum-infected mice.

    abstract::Neospora caninum is a coccidian parasite causative of clinical infections in a wide range of animal hosts. The maturation and activation of splenic conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) were studied here in BALB/c mice challenged intraperitoneal with N. caninum tachyzoites. The nu...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2009.65

    authors: Teixeira L,Botelho AS,Mesquita SD,Correia A,Cerca F,Costa R,Sampaio P,Castro AG,Vilanova M

    更新日期:2010-01-01 00:00:00

  • A model for the regulation of follicular dendritic cells predicts invariant reciprocal-time decay of post-vaccine antibody response.

    abstract::Follicular dendritic cells (FDC) play a crucial role in the regulation of humoral immunity. They are believed to be responsible for long-term persistence of antibody, due to their role in antibody response induction and their ability to retain antigen in immunogenic form for long periods. In this article, a regulatory...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2017.55

    authors: Almudevar A

    更新日期:2017-10-01 00:00:00

  • A CD2 high-expressing stress-resistant human plasmacytoid dendritic-cell subset.

    abstract::Human plasmacytoid dendritic cells (pDCs) were considered to be a phenotypically and functionally homogeneous cell population; however, recent analyses indicate potential heterogeneity. This is of major interest, given their importance in the induction of anti-viral responses and their role in creating immunologically...

    journal_title:Immunology and cell biology

    pub_type: 杂志文章

    doi:10.1038/icb.2015.116

    authors: Bryant C,Fromm PD,Kupresanin F,Clark G,Lee K,Clarke C,Silveira PA,Suen H,Brown R,Newman E,Cunningham I,Ho PJ,Gibson J,Bradstock K,Joshua D,Hart DNj

    更新日期:2016-05-01 00:00:00