Abstract:
:Keeping track of individual cell identifications is imperative to the study of dynamic single-cell behavior over time. Highly motile hematopoietic stem and progenitor cells (HSPCs) migrate quickly and do not adhere, and thus must be imaged very frequently to keep cell identifications. Even worse, they are also flushed away during medium exchange. To overcome these limitations, we tested antibody coating for reducing HSPC motility in vitro. Anti-CD43- and anti-CD44-antibody coating reduced the cell motility of mouse and human HSPCs in a concentration-dependent manner. This enables 2-dimensional (2D) colony formation without cell mixing in liquid cultures, massively increases time-lapse imaging throughput, and also maintains cell positions during media exchange. Anti-CD43 but not anti-CD44 coating reduces mouse HSPC proliferation with increasing concentrations. No relevant effects on cell survival or myeloid and megakaryocyte differentiation of hematopoietic stem cells and multipotent progenitors 1-5 were detected. Human umbilical cord hematopoietic CD34+ cell survival, proliferation, and differentiation were not affected by either coating. This approach both massively simplifies and accelerates continuous analysis of suspension cells, and enables the study of their behavior in dynamic rather than static culture conditions over time.
journal_name
Bloodjournal_title
Bloodauthors
Loeffler D,Wang W,Hopf A,Hilsenbeck O,Bourgine PE,Rudolf F,Martin I,Schroeder Tdoi
10.1182/blood-2017-07-794131subject
Has Abstractpub_date
2018-03-29 00:00:00pages
1425-1429issue
13eissn
0006-4971issn
1528-0020pii
blood-2017-07-794131journal_volume
131pub_type
杂志文章相关文献
BLOOD文献大全abstract::The paradoxical correlation between thrombosis and the lupus anticoagulant (LAC) effect is an enigmatic feature of the antiphospholipid (aPL) syndrome. The Dutch authors previously reported that thrombosis-related anti-beta2-glycoprotein I (beta2GPI) antibodies recognize domain I and cause LAC. The American authors re...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-07-030148
更新日期:2007-02-15 00:00:00
abstract::An electroimmunoassay was applied to the quantitation of platelet-associated IgG (PAIgG). Protein solubilized by Triton X100 from washed platelets was electrophoresed at pH 5.0 in agarose gels containing carbamoylated rabbit anti-human IgG (pI approximately equal to 5.0). Because the rabbit antibody is immobilized und...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Platelet endothelial cell adhesion molecule 1 (PECAM-1) is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution. This study investigated the effect of PECAM-1 deficiency on thrombus resolution in FVB/n mice and the extent to which levels of soluble PECAM-1 (sPECAM-1)...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-04-499558
更新日期:2013-11-07 00:00:00
abstract::We have previously shown that coculture of human embryonic stem cells (hESCs) for 14 days with immortalized fetal hepatocytes yields CD34(+) cells that can be expanded in serum-free liquid culture into large numbers of megaloblastic nucleated erythroblasts resembling yolk sac-derived cells. We show here that these pri...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-07-102087
更新日期:2008-02-15 00:00:00
abstract::B-cell chronic lymphocytic leukemia (B-CLL) is defined by the accumulation of CD5(+) B cells in the periphery and bone marrow. This disease is not characterized by highly proliferative cells but rather by the presence of leukemic cells with significant resistance to apoptosis and, therefore, prolonged survival. B-lymp...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-02-0558
更新日期:2002-10-15 00:00:00
abstract::We genotyped 370 subjects with primary myelofibrosis (PMF) and 148 with postpolycythemia vera/postessential thrombocythemia (PPV/PET) MF for mutations of EZH2. Mutational status at diagnosis was correlated with hematologic parameters, clinical manifestations, and outcome. A total of 25 different EZH2 mutations were de...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-06-363424
更新日期:2011-11-10 00:00:00
abstract::Fluid shear stress generated by steady laminar blood flow protects vessels from atherosclerosis. Krüppel-like factor 2 (KLF2) and endothelial nitric oxide synthase (eNOS) are fluid shear stress-responsive genes and key mediators in flow anti-inflammatory and antiatherosclerotic actions. However, the molecular mechanis...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-05-224824
更新日期:2010-04-08 00:00:00
abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is a rare bone marrow failure disorder that manifests with hemolytic anemia, thrombosis, and peripheral blood cytopenias. The absence of two glycosylphosphatidylinositol (GPI)-anchored proteins, CD55 and CD59, leads to uncontrolled complement activation that accounts for hemol...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2014-02-522128
更新日期:2014-10-30 00:00:00
abstract::Red blood cell (RBC) and platelet transfusion requirements in patients given nonmyeloablative versus conventional peripheral blood stem cell (PBSC) transplants from HLA-matched siblings were compared. Between December 1997 and March 2000, 40 patients, aged 21 to 67 years (median 51), with hematologic malignancies unde...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood.v98.13.3584
更新日期:2001-12-15 00:00:00
abstract::The CD31 monoclonal antibody, LYP21, binds to the CD31 domain 6 and inhibits the human mixed-lymphocyte reaction (MLR) in a specific and dose-dependent fashion. A synthetic CD31 peptide based on human CD31 epitope (amino acids 551 to 574) recognized by LYP21 is equally effective in inhibiting the MLR. In this study, w...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-02-15 00:00:00
abstract::Chromosome 11q deletions are frequently observed in chronic lymphocytic leukemia (CLL) in association with progressive disease and a poor prognosis. A minimal region of deletion has been assigned to 11q22-q23. Trinucleotide repeats have been associated with anticipation in disease, and evidence of anticipation has bee...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.2.509
更新日期:2001-01-15 00:00:00
abstract::In search of a possible involvement of the human herpesvirus type 6 (HHV-6) in human Hodgkin's and non-Hodgkin's lymphomas, we studied the levels of anti-HHV-6 antibodies in the sera of 94 cases by an immunofluorescence assay, as well as the presence of HHV-6 sequences in the affected tissues of 66 cases by polymerase...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-05-15 00:00:00
abstract::The spatial distribution of subpopulations of hemopoietic progenitor cells following syngeneic transplantation was investigated at the single-cell level. The location of infused hemopoietic progenitor cells within the femoral bone marrow of nonablated recipients was determined by 5-(and-6)-carboxyfluorescein diacetate...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.8.2293
更新日期:2001-04-15 00:00:00
abstract::Eph receptors and their ephrin ligands are involved in normal hematopoietic development and tumorigenesis. Using methylated CpG island amplification/DNA promoter microarray, we identified several EPH receptor and EPHRIN genes as potential hypermethylation targets in acute lymphoblastic leukemia (ALL). We subsequently ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-05-222208
更新日期:2010-03-25 00:00:00
abstract::Hyperresponsiveness to growth factors underlies a wide variety of human diseases,including hemangiomas, the most common tumor of childhood. Hemangiomas have been found to be clonal neoplasms of endothelial cells, with somatic mutations in unknown genes likely to be responsible for their development. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-04-415059
更新日期:2012-05-17 00:00:00
abstract::Notch signaling has been shown to play a key role in cell fate decisions in numerous developmental systems. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we reported the expression of human Notch-1 in CD34+ progenitors. In this study, we evaluated the expression of human Notch-1 and Notch-2 p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::There have been no studies on patient outcome after allogeneic hematopoietic cell transplantation (HCT) in patients with X-linked inhibitor of apoptosis (XIAP) deficiency. To estimate the success of HCT, we conducted an international survey of transplantation outcomes. Data were reported for 19 patients. Seven patient...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-06-432500
更新日期:2013-02-07 00:00:00
abstract::We used in vitro DNA amplification by the polymerase chain reaction and nonradioactive probes for prenatal diagnosis of beta thalassemia in Chinese from the Guangdong province. Exact molecular diagnoses were made in all 20 fetuses studied over a 6-month period. We conclude that this method of prenatal diagnosis for be...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Autoimmune lymphoproliferative syndrome (ALPS) is a disorder of abnormal lymphocyte survival caused by defective Fas-mediated apoptosis, leading to lymphadenopathy, hepatosplenomegaly, and an increased number of double-negative T cells (DNTs). Treatment options for patients with ALPS are limited. Rapamycin has been sh...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-01-010124
更新日期:2006-09-15 00:00:00
abstract::Membrane markers expressed by a transplantable granulocytic leukemia induced in inbred strain 13 guinea pigs by N-nitroso-N-butylurea have been investigated by serologic and immunochemical methods. Although the leukemic myeloblasts have no detectable surface immunoglobulin or Fc receptors, they have been found to synt...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract::Sickle red blood cells (RBC) become dehydrated as a consequence of potassium loss. This process depends at least partly on deoxygenation and may be influenced by the presence of oxygenation/deoxygenation cycles and the frequency of cycling. In this study, sickle RBC were subjected to approximately 180 oxygenation/deox...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-03-15 00:00:00
abstract::Inhibitors of cyclin-dependent kinases (Cdks) have been reported to have activities in chronic lymphocytic leukemia cells by inhibiting Cdk7 and Cdk9, which control transcription. Here we studied the novel Cdk inhibitor SNS-032, which exhibits potent and selective inhibitory activity against Cdk2, Cdk7, and Cdk9. We h...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-12-190256
更新日期:2009-05-07 00:00:00
abstract::Cylindromatosis (CYLD) is a deubiquitinase that was initially identified as a tumor suppressor and has recently been implicated in diverse normal physiologic processes. In this study, we have investigated the involvement of CYLD in angiogenesis, the formation of new blood vessels from preexisting ones. We find that kn...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-10-248526
更新日期:2010-05-20 00:00:00
abstract::Recent evidence suggests that proteolysis plays an important role in some forms of inherited and acquired von Willebrand disease (vWD). Because calpains and one or more enzymes released from polymorphonuclear leukocytes are known to proteolyze von Willebrand factor (vWF) in vitro with resultant loss of large multimers...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::The prognostic implications of t(9;22)(q34;q11) were assessed at a median follow-up of 3.5 years in 434 children receiving intensive treatment for acute lymphoblastic leukemia (ALL). Four-year event-free and overall survivals were 81% and 88%, respectively, in 419 children lacking t(9;22), but were 0% and 20%, respect...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1991-02-01 00:00:00
abstract::The human CD34 surface antigen is selectively expressed on stem/progenitor cells within the hematopoietic system. Because CD34 expression is tightly linked to the immature status of hematopoietic cells, with expression being rapidly lost as hematopoietic cells mature and differentiate, an examination of its regulation...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Recent advances in molecular biology have led to the identification of hematopoietic growth factors that support and influence the proliferation of hematopoietic progenitor cells in vitro and in vivo. Although these factors have been extensively studied, little is known of their role in the regulation of cell-cycling ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Protein disulfide isomerase (PDI) derived from intravascular cells is required for thrombus formation. However, it remains unclear whether platelet PDI contributes to the process. Using platelet-specific PDI-deficient mice, we demonstrate that PDI-null platelets have defects in aggregation and adenosine triphosphate s...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-03-492504
更新日期:2013-08-08 00:00:00
abstract::Screening for hereditary hemochromatosis (HHC) by means of transferrin saturation (TS) levels has been advocated and will identify many patients who are asymptomatic. The purposes of this study were (1) to determine HFE genotypes among asymptomatic HHC patients and correlate this profile with the degree of iron overlo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Based on our previous demonstration that quinine could be used clinically to reverse P-glycoprotein-mediated resistance, we designed a multicenter, randomized trial aiming to determine whether quinine would improve the survival of adult patients (15-60 years old) with de novo acute myelogenous leukemia (AML). These pa...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1182/blood-2002-11-3419
更新日期:2003-08-15 00:00:00