Abstract：
:Endothelial cell proliferation is a key process during vascular growth but its kinetics could only be assessed in vitro or ex vivo so far. To enable the monitoring and quantification of cell cycle kinetics in vivo, we have generated transgenic mice expressing an eGFP-anillin construct under control of the endothelial-specific Flt-1 promoter. This construct labels the nuclei of endothelial cells in late G1, S and G2 phase and changes its localization during the different stages of M phase, thereby enabling the monitoring of EC proliferation and cytokinesis. In Flt-1/eGFP-anillin mice, we found eGFP+ signals specifically in Ki67+/PECAM+ endothelial cells during vascular development. Quantification using this cell cycle reporter in embryos revealed a decline in endothelial cell proliferation between E9.5 to E12.5. By time-lapse microscopy, we determined the length of different cell cycle phases in embryonic endothelial cells in vivo and found a M phase duration of about 80 min with 2/3 covering karyokinesis and 1/3 cytokinesis. Thus, we have generated a versatile transgenic system for the accurate assessment of endothelial cell cycle dynamics in vitro and in vivo.
journal_name
Angiogenesisjournal_title
Angiogenesisauthors
Herz K,Becker A,Shi C,Ema M,Takahashi S,Potente M,Hesse M,Fleischmann BK,Wenzel Ddoi
10.1007/s10456-018-9601-1subject
Has Abstractpub_date
2018-05-01 00:00:00pages
349-361issue
2eissn
0969-6970issn
1573-7209pii
10.1007/s10456-018-9601-1journal_volume
21pub_type
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