New methods for determining proton pumping ability and electron transfer activity of the cytochrome bc1 complex.

Abstract:

:It is important to determine the electron transfer activity and proton pumping ability of the cytochrome bc1 complex for better understanding its structure and function. In this study, several methods for determining the electron transfer and proton pumping of the bc1 complex, including the traditional and the new methods, are presented and evaluated. For determining the proton pumping ability of the bc1 complex, the new stopped-flow method has a higher accuracy than the traditional pH meter method, and the new spectrophotometer method is more convenient than the traditional pH meter method. In measuring the electron transfer activity of the bc1 complex, the new stopped-flow method is more accurate and has a higher separating capacity than the traditional spectrophotometer method.

authors

Qu Y,Dong F

doi

10.1093/abbs/gmu126

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

114-20

issue

2

eissn

1672-9145

issn

1745-7270

pii

gmu126

journal_volume

47

pub_type

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