Abstract：
:Acanthamoeba isolated from environmental soil harbors the obligate intracellular symbiont Neochlamydia, which has a critical role in host amoebal defense against Legionella pneumophila infection. Here, by using morphological analysis with confocal laser scanning fluorescence microscopy and transmission electron microscopy, proteome analyses with two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and liquid chromatography-mass spectrometry (LC/MS), and transcriptome analysis with DNA microarray, we explored the mechanism by which the Neochlamydia affected this defense. We observed that when rare uptake did occur, the symbiotic amoebae allowed Legionella to grow normally. However, the symbiotic amoebae had severely reduced uptake of Legionella when compared with the aposymbiotic amoebae. Also, in contrast to amoebae carrying the endosymbiont, the actin cytoskeleton was significantly disrupted by Legionella infection in aposymbiotic amoebae. Furthermore, despite Legionella exposure, there was little change in Neochlamydia gene expression. Taken together, we concluded that the endosymbiont, Neochlamydia prevents Legionella entry to the host amoeba, resulting in the host defense against Legionella infection.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Maita C,Matsushita M,Miyoshi M,Okubo T,Nakamura S,Matsuo J,Takemura M,Miyake M,Nagai H,Yamaguchi Hdoi
10.1016/j.micinf.2017.12.012subject
Has Abstractpub_date
2018-04-01 00:00:00pages
236-244issue
4eissn
1286-4579issn
1769-714Xpii
S1286-4579(18)30001-7journal_volume
20pub_type
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