Abstract:
:Although there have been numerous attempts to develop a successful vaccine against leishmaniasis, based on the clinical trial in this field, no vaccine against Leishmania in routine way can be found for globally effective vaccination in human. Amongst, first generation vaccines consisting of parasite fractions or whole killed Leishmania showed more successful results in clinical trials. It seems that the main reason for the low efficacy of these vaccines is lack of a suitable adjuvant. In this study, a crude extract of detergent-solubilized L. major promastigotes as a novel developed antigen (whole Leishmania lysate (WLL)) was formulated in liposomal form. The cationic liposomes consisting of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used to deliver WLL. Liposomes formulations containing different WLL concentrations (prepared from 103, 104, 105, 106 and 107 parasites) were prepared and characterized for particle size, surface charge, proteins, DNA and phospholipids contents. Moreover, to explore the type of immune response generated and extend of immunization, in vivo and in vitro tests including evaluation of lesion development, parasite burden in the foot and spleen, Th1 and Th2 cytokine analysis, and titration of IgG isotypes before and after the challenge were used. The maximum immunization was provided by WLL06 as depicted by the reduction of footpad swelling andparasite load, increase in anti-Leishmania IgG2a production, though no significant difference was observed between mice which received WLL05 vs WLL06. While maximum immunization was seen in WLL06 group, most of the liposomal WLL formulations induced a mixed Th1/Th2 response. Hence, a more protective immune response is expected to be induced when an immune potentiator adjuvant such as CpG ODNs would be co-deliverd in WLL liposomal formulations.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Jafari I,Heravi Shargh V,Shahryari M,Abbasi A,Jaafari MR,Khamesipour A,Badiee Adoi
10.1016/j.imbio.2017.12.003subject
Has Abstractpub_date
2018-01-01 00:00:00pages
493-500issue
6-7eissn
0171-2985issn
1878-3279pii
S0171-2985(17)30216-4journal_volume
223pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::IL-33 targeting ST2 receptor (T1/ST2), expressed on Th2 cell surface, regulates the production of cytokines like IL-17A and IL-31. We studied the role of IL-33/ST2 axis in IL-31 and IL-17A production in patients with allergic rhinitis (AR) and with concomitant allergic asthma and rhinitis (AAR). 20 healthy control sub...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.02.005
更新日期:2015-08-01 00:00:00
abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.12.005
更新日期:2011-12-16 00:00:00
abstract::In a rat model, we compared the effects of various soluble products released by T cells, such as the unspecifically acting gamma-interferon or the newly detected alloantigen-induced factors that specifically act on nonlymphoid, hemopoietic bone marrow cells. We found two types of reactivity patterns with regard to the...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(87)80096-7
更新日期:1987-12-01 00:00:00
abstract::This study demonstrates immune complexes in thyroid glands of Obese strain (OS) chickens, that consist of thyroglobulin (Tg) and antibodies to Tg. In IIF tests it was shown that these complexes fix complement with an age-dependent increase from 12% in 19-day-old embryos up to 100% in 6-week-old animals. This finding a...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(83)80035-7
更新日期:1983-05-01 00:00:00
abstract::The distribution of membrane-associated nucleotide pyrophosphatase activity has been investigated on mouse leucocytes of different origin. It is shown that enzyme-specific activity is higher on bone-marrow cells than on cells derived from peripheral organs. Since thymus lymphocytes show the lowest activity, it is obvi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80029-X
更新日期:1982-01-01 00:00:00
abstract::Kinetic methods can provide significant information concerning the mechanism of cellular cytotoxicity reactions. Previous kinetic studies of cytotoxic T lymphocytes (Tc) have been hampered by the heterogeneity of the effector cell population tested. We therefore examined the kinetics of lysis mediated by cloned, IL 2 ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(88)80065-2
更新日期:1988-12-01 00:00:00
abstract::Intestinal epithelial cells produce cytokines in response to bacterial peptidoglycan (PGN), which is detected by several classes of pattern-recognition receptors (PRRs) as peptidoglycan recognition proteins (PGlyRPs), Toll-like receptor 2 (TLR2) and NOD receptors. All types of PGlyRPs recognize bacterial peptidoglycan...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.10.013
更新日期:2012-04-01 00:00:00
abstract::In contrast to other pneumococcal serotypes, which are thought to be T-independent antigens, type 1 Streptococcus pneumoniae polysaccharide (Sp1) is a zwitterionic polysaccharide (ZPS). It has previously been shown to be processed and presented by antigen-presenting cells utilizing the MHC-II pathway, which leads to S...
journal_title:Immunobiology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.imbio.2012.05.008
更新日期:2013-03-01 00:00:00
abstract::Based on the similar effects that HIV-1 gp41 like human type I interferons (IFN-alpha and -beta) upregulated MHC expression and inhibited lymphocyte proliferation, we compared amino acid sequences of gp41 and human type I interferons (IFN-alpha and -beta), and found sequence-similarity existing between the immunosuppr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(98)80043-0
更新日期:1998-02-01 00:00:00
abstract::The concept of using mesenchymal stem cells (MSCs) in bone repair has progressively evolved, and the goal of cell-mediated therapy is to prolong the natural physiological abilities of healing, or substitute them, when these are lacking, failing, or progressing too slowly. The future application of MSCs in human therap...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2008.07.030
更新日期:2009-01-01 00:00:00
abstract::We examined the expression of seven V alpha or V beta T cell receptor (TCR) segments on human CD4+ and CD8+ T cells. Confirming previously published results, we found a preferential expression of four V segment gene products on CD4+ T cells. One of these markers (V beta 6.7) was constantly expressed on more CD4+ T cel...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80242-1
更新日期:1993-01-01 00:00:00
abstract::In contrast to murine spleen M phi, resident peritoneal M phi from health mice express very little Forssman glycolipid antigen (Fo). The following experiments suggest that Fo expression by peritoneal M phi may be associated with inflammation. Balb/c and CBA/J mice were given inflammatory stimuli by i.p. injection of l...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80302-5
更新日期:1990-06-01 00:00:00
abstract::Understanding the mechanisms governing the type of induced immune response after microbial invasion, could be of crucial importance for the rational design of a bacteria-based vaccine. Targeting a vaccine directly to dendritic cells (DCs), which are considered the most powerful antigen presenting cells, could be extre...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00094
更新日期:2001-12-01 00:00:00
abstract::We studied immunohistological and biochemical aspects of the CD44 molecule with a mAb produced in our lab: GRHL-1. The characteristic expression of this antigen in cells of B lineage was analyzed. This mAb showed identical immunohistological patterns of reactivity to other mAbs included in CD44 cluster, on a variety l...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80181-6
更新日期:1991-09-01 00:00:00
abstract::Immunological mechanisms of hygiene hypothesis are expected to develop a novel strategy for allergy prevention. Although a large number of studies has investigated the relation between allergies and infection, little is known about the influence of the exposure to infections on antigen uptake by dendritic cells (DCs)....
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.10.018
更新日期:2018-01-01 00:00:00
abstract::The immunoglobulin production capacities of peripheral blood lymphocytes obtained from patients with various chronic inflammatory liver diseases and from normal individuals were compared. Using a reverse hemolytic plaque assay, immunoglobulin-secreting cells (ISC) were counted immediately after isolation (immediate IS...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(88)80066-4
更新日期:1988-12-01 00:00:00
abstract::Hepatitis B virus (HBV) continues to be a serious worldwide health problem despite the use of protective HBV vaccines and therapeutic regimens against chronic HBV infection. Chronic HBV patients cannot induce sufficient immune responses against the virus. HBV and its antigens are believed to suppress immune responses ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.07.020
更新日期:2014-12-01 00:00:00
abstract::We studied local events in the popliteal lymph nodes of CD4-deficient mice following foot pad injection with an MMTV strain which carries the gene for a V beta 14-specific superantigen. Injection of the V beta 14-specific MMTV induced vigorous expansion of V beta 14+ CD4+ T cells and B cells in their lymph nodes of CD...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(96)80053-2
更新日期:1996-08-01 00:00:00
abstract::The alarming rise of obesity and type 2 diabetes (T2D) has put a tremendous strain on global healthcare systems. Over the past decade extensive research has focused on the role of macrophages as key mediators of inflammation in T2D. The inflammatory environment in the obese adipose tissue and pancreatic β-cell islets ...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2016.08.011
更新日期:2017-10-01 00:00:00
abstract::Using irradiation bone marrow chimeras to analyze restriction specificity of helper T cells, we found that recipient H-2 type dictated the H-2 type which the T cells recognize as self (adaptive differentiation). T cells from (H-2b----H-2k) chimeras cooperate with non-T cells bearing Iak to generate a vigorous PFC resp...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(85)80054-1
更新日期:1985-02-01 00:00:00
abstract::Cytokines are important mediators of the effects observed after the administration of endotoxin. One of them, tumor necrosis factor, is particularly important since it plays a cardinal role in two major endotoxin activities: its antitumor effect and its capacity to induce a systemic inflammatory response syndrome. TNF...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/S0171-2985(11)80347-5
更新日期:1993-04-01 00:00:00
abstract::Immunocytochemical studies on both the light-microscopic and electron-microscopic levels proved that peritoneal resident macrophages derive from a precursor cell in the bone marrow. Evidence that this precursor cell differs from that of the monocyte is described. Progenitor cells of peritoneal resident macrophages wer...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/S0171-2985(82)80075-2
更新日期:1982-04-01 00:00:00
abstract::The leukocyte integrins CD11a/CD18 (LFA-1, alphaLbeta2) and CD49d (VLA-4, alpha4beta1, alpha4beta7) mediate leukocyte transendothelial migration during immune and inflammatory responses and provides co-stimulatory signals for the activation of T lymphocytes. Our previous studies demonstrate that the CD11a gene promote...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2005.05.008
更新日期:2005-01-01 00:00:00
abstract::L-alanine was shown earlier to play a significant role for the proliferation of lymphocytes in vitro. In the present work the effect of L-alanine and some other amino acids on thymocyte proliferation was studied in vivo by local administration into one thymus lobe of guinea pigs. Proliferating cells were pulse labelle...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80487-0
更新日期:1993-06-01 00:00:00
abstract:BACKGROUND:T helper cells can differentiate into several subsets of T lymphocytes, including Th1, Th2, and regulatory T (Treg) cells. As a result of this ability to differentiate, the corresponding T cell receptor (TCR) spectra display considerable cellular plasticity and interchangeability. In contrast, T lymphocyte d...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.02.007
更新日期:2017-07-01 00:00:00
abstract::Regulation of an immune response requires complex crosstalk between cells of the innate and adaptive immune systems, via both cell-cell contact and secretion of cytokines. An important cytokine with a broad regulatory role in the immune system is transforming growth factor-β (TGF-β). TGF-β is produced by and has effec...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2012.06.009
更新日期:2012-12-01 00:00:00
abstract::Complement component C6 is a part of the lytic membrane attack complex formed during complement activation. Animal modeling to define the role of C5a vs. C5b-9 in human disease has used rodents deficient in C6, yet the molecular basis for the deficiencies has not been ascertained. Oligonucleotides derived from a 493 b...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2004.08.001
更新日期:2004-01-01 00:00:00
abstract::Based on our findings that HIV-1 gp41 independently of CD4 can bind to the human helper T lymphoid cell line H9, B cell line Raji and monocyte cell line U937, we characterized putative binding of HIV-1 gp41 to human peripheral blood lymphocytes (PBLs) and monocytes. Using flow cytometry (FACS), we demonstrated that th...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80216-0
更新日期:1993-08-01 00:00:00
abstract::Regulatory properties of macrophages associated with alternative activation serve to limit the exaggerated inflammatory response during pneumonia caused by Pseudomonas aeruginosa infection. Arginase-1 is an important effector of these macrophages believed to play an essential role in decreasing injury and promoting re...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2020.152034
更新日期:2020-11-24 00:00:00
abstract::Visceral leishmaniasis (VL) caused by Leishmania donovani persists as a major public health issue in tropical and subtropical areas of the world. Current treatment of this disease relies on use of drugs. It is doubtful that chemotherapy can alone eradicate the disease, so there is a need for an effective vaccine. Kill...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.05.014
更新日期:2015-09-01 00:00:00