Control of neonatal human dermal fibroblast migration on poly(lactic-co-glycolic acid)-coated surfaces by electrotaxis.

Abstract:

:Many types of cells respond to applied direct current electric fields (dcEFs) by directional cell migration, a phenomenon called galvanotaxis or electrotaxis. In this study, electrotaxis was used to control cell migration. We designed a new electrotaxis incubator and chamber system to facilitate long-term (> 12 h) observation and to allow for alterations to the direction of the current. Poly(lactic-co-glycolic acid) (PLGA) was coated onto surfaces to mimic a commonly used tissue-engineering scaffolding environment. Neonatal human dermal fibroblasts (nHDFs) were grown on PLGA-coated surfaces and exposed to EFs at increasing currents in the range 0-1 V/cm. These cells migrated toward the cathode during 3 h of dcEF stimulation; however, the migration speed decreased with increasing electric fields. Cells exposed to dcEFs in the range 1-2 V/cm showed no changes to migration speed or x forward migration indices (xFMIs) and the cells continued to move toward the cathode. nHDFs showed directional migration towards the cathode in direct current (dc) EFs (1 V/cm) and they moved in the opposite direction when the polarity of the dcEF was reversed. Reorganization of the actin cytoskeleton and polarization of the Golgi apparatus were evaluated by immunostaining, which showed that the actin cytoskeleton elongated towards the cathode and the Golgi apparatus polarized in the direction of the dcEF. This study revealed that cell migration could potentially be controlled on PLGA scaffolds through electrotaxis. Copyright © 2015 John Wiley & Sons, Ltd.

journal_name

J Tissue Eng Regen Med

authors

Kim MS,Lee MH,Kwon BJ,Seo HJ,Koo MA,You KE,Kim D,Park JC

doi

10.1002/term.1986

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

862-868

issue

3

eissn

1932-6254

issn

1932-7005

journal_volume

11

pub_type

杂志文章
  • Biodegrading highly porous elastomeric graft regenerates muscular and innervated carotid artery-Comparative study with vein graft.

    abstract::This study aims to investigate the superiorities of fast degrading elastomeric poly(glycerol sebacate) (PGS)/polycaprolactone (PCL) grafts over autologous vein grafts in the reconstruction of carotid artery, thus providing more suitable vascular grafts for carotid artery replacement. We fabricated small arterial graft...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2856

    authors: Yang X,Gao Z,Liu H,Wu W

    更新日期:2019-07-01 00:00:00

  • Generation of tunable glycosaminoglycan hydrogels to mimic extracellular matrices.

    abstract::Biomaterials and, especially, scaffolds may function as temporary extracellular matrix (ECM), mimicking in vivo environmental structures and facilitating cell growth and tissue regeneration. ECM is composed mostly of glycosaminoglycans (GAGs) and proteins, the ratio of GAGs, hyaluronic acid (HA):sulphated GAGs (sGAGs)...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1883

    authors: Herrero-Mendez A,Palomares T,Castro B,Herrero J,Alonso-Varona A

    更新日期:2016-12-01 00:00:00

  • A novel culture device for the evaluation of three-dimensional extracellular matrix materials.

    abstract::Cell-matrix interactions in a three-dimensional (3D) extracellular matrix (ECM) are of fundamental importance in living tissue, and their in vitro reconstruction in bioartificial structures represents a core target of contemporary tissue engineering concepts. For a detailed analysis of cell-matrix interaction under hi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1550

    authors: Akhyari P,Ziegler H,Gwanmesia P,Barth M,Schilp S,Huelsmann J,Hoffmann S,Bosch J,Kögler G,Lichtenberg A

    更新日期:2014-09-01 00:00:00

  • Bone tissue engineering in oral peri-implant defects in preclinical in vivo research: A systematic review and meta-analysis.

    abstract::The regeneration and establishment of osseointegration within oral peri-implant bone defects remains a clinical challenge. Bone tissue engineering (BTE) is emerging as a promising alternative to autogenous and/or biomaterial-based bone grafting. The objective of this systematic review was to answer the focused questio...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,meta分析,评审

    doi:10.1002/term.2412

    authors: Shanbhag S,Pandis N,Mustafa K,Nyengaard JR,Stavropoulos A

    更新日期:2018-01-01 00:00:00

  • Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications.

    abstract::Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum techno...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1979

    authors: Lange P,Greco K,Partington L,Carvalho C,Oliani S,Birchall MA,Sibbons PD,Lowdell MW,Ansari T

    更新日期:2017-03-01 00:00:00

  • Continuous supply of TGFβ3 via adenoviral vector promotes type I collagen and viability of fibroblasts in alginate hydrogel.

    abstract::In recent years, transforming growth factor-β3 (TGFβ3) has interested more and more researchers with its competence in engineered histogenesis. In the present study we employed recombinant adenoviral vectors to deliver the constitutively active TGFβ3 gene to human dermal fibroblasts, which could maintain the continuou...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.263

    authors: Yao Y,Zhang F,Zhou R,Li M,Wang DA

    更新日期:2010-10-01 00:00:00

  • Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering.

    abstract::In the context of prevascularization strategies for tissue-engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2475

    authors: Dohle E,El Bagdadi K,Sader R,Choukroun J,James Kirkpatrick C,Ghanaati S

    更新日期:2018-03-01 00:00:00

  • Qualifying stem cell sources: how to overcome potential pitfalls in regenerative medicine?

    abstract::Regenerative medicine aims to replace lost cells and to restore damaged tissues and organs by either tissue-engineering approaches or stimulation of endogenous processes. Due to their biological properties, stem cells promise to be an effective source for such strategies. Especially adult multipotent stem cells (ASCs)...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.1923

    authors: Reinke S,Dienelt A,Blankenstein A,Duda GN,Geissler S

    更新日期:2016-01-01 00:00:00

  • Synergistic effects of low-level laser and mesenchymal stem cells on functional recovery in rats with crushed sciatic nerves.

    abstract::Transplantation of mesenchymal stem cells (MSCs) has been proposed to exert beneficial effects on peripheral nerve regeneration after a peripheral nerve injury, but the functional recovery in the denervated limb is still limited. In this study, we used low-level laser therapy (LLLT) as an adjunct therapy for MSC trans...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1714

    authors: Yang CC,Wang J,Chen SC,Hsieh YL

    更新日期:2016-02-01 00:00:00

  • The power of fat and its adipose-derived stromal cells: emerging concepts for fibrotic scar treatment.

    abstract::Lipofilling or lipografting is a novel and promising treatment method for reduction or prevention of dermal scars after injury. Ample anecdotal evidence from case reports supports the scar-reducing properties of adipose tissue grafts. However, only a few properly controlled and designed clinical trials have been condu...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2213

    authors: Spiekman M,van Dongen JA,Willemsen JC,Hoppe DL,van der Lei B,Harmsen MC

    更新日期:2017-11-01 00:00:00

  • New observations of the hierarchical structure of human enamel, from nanoscale to microscale.

    abstract::Microstructure in terms of hierarchical assembly exists widely in mineralized biomaterials, fulfilling an important role in setting up their outstanding properties. The purpose of this study was to investigate the hierarchical assembly of enamel structure and functions, which are related to the unique characteristics ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.21

    authors: Cui FZ,Ge J

    更新日期:2007-05-01 00:00:00

  • Cranial reconstruction using allogeneic mesenchymal stromal cells: A phase 1 first-in-human trial.

    abstract::Cranioplasty is necessary for patients that have undergone craniectomy following trauma, stroke or other causes of elevated intracranial pressure. This study assessed the effectiveness of treating cranial defects with allogeneic mesenchymal stromal cells (MSC) on a ceramic carrier and polymer scaffold, to produce viab...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type:

    doi:10.1002/term.2459

    authors: Morrison DA,Kop AM,Nilasaroya A,Sturm M,Shaw K,Honeybul S

    更新日期:2018-02-01 00:00:00

  • Vibration stimulates vocal mucosa-like matrix expression by hydrogel-encapsulated fibroblasts.

    abstract::The composition and organization of the vocal fold extracellular matrix (ECM) provide the viscoelastic mechanical properties that are required to sustain high-frequency vibration during voice production. Although vocal injury and pathology are known to produce alterations in matrix physiology, the mechanisms responsib...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.219

    authors: Kutty JK,Webb K

    更新日期:2010-01-01 00:00:00

  • Development of decellularized scaffolds for stem cell-driven tissue engineering.

    abstract::Organ transplantation is an effective treatment for chronic organ dysfunctioning conditions. However, a dearth of available donor organs for transplantation leads to the death of numerous patients waiting for a suitable organ donor. The potential of decellularized scaffolds, derived from native tissues or organs in th...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2061

    authors: Rana D,Zreiqat H,Benkirane-Jessel N,Ramakrishna S,Ramalingam M

    更新日期:2017-04-01 00:00:00

  • Virus immobilization on biomaterial scaffolds through biotin-avidin interaction for improving bone regeneration.

    abstract::To spatially control therapeutic gene delivery for potential tissue engineering applications, a biotin-avidin interaction strategy was applied to immobilize viral vectors on biomaterial scaffolds. Both adenoviral vectors and gelatin sponges were biotinylated and avidin was applied to link them in a virus-biotin-avidin...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1774

    authors: Hu WW,Wang Z,Krebsbach PH

    更新日期:2016-02-01 00:00:00

  • Label-free relative quantification of secreted proteins as a non-invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair.

    abstract::Cartilage tissue engineering is making progress, but the competing available strategies still leave room for improvement and consensual overviews regarding the best combinations of scaffolds and cell sources are limited by the capacity to compare them directly. In addition, because most strategies involve autologous c...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2454

    authors: Henrionnet C,Gillet P,Mainard D,Vincourt JB,Pinzano A

    更新日期:2018-03-01 00:00:00

  • Coating bone-like apatite onto organic substrates using solutions mimicking body fluid.

    abstract::Bone-like apatite is a carbonated hydroxyapatite with a small crystallite and low crystallinity. The formation of a layer consisting of bone-like apatite is an essential condition for bioactive materials to achieve direct bonding with living bone. A bone-like apatite layer can be formed on the surface of organic subst...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.3

    authors: Ohtsuki C,Kamitakahara M,Miyazaki T

    更新日期:2007-01-01 00:00:00

  • In vitro biocompatibility of 45S5 Bioglass-derived glass-ceramic scaffolds coated with poly(3-hydroxybutyrate).

    abstract::The aim of this work was to study the in vitro biocompatibility of glass-ceramic scaffolds based on 45S5 Bioglass, using a human osteosarcoma cell line (HOS-TE85). The highly porous scaffolds were produced by the foam replication technique. Two different types of scaffolds with different porosities were analysed. They...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.150

    authors: Bretcanu O,Misra S,Roy I,Renghini C,Fiori F,Boccaccini AR,Salih V

    更新日期:2009-02-01 00:00:00

  • Benefits of concurrent delivery of hyaluronan and IGF-1 cues to regeneration of crosslinked elastin matrices by adult rat vascular cells.

    abstract::Elastin, a major component of vascular matrices, critically determines vascular mechanics and maintains the quiescence of smooth muscle cells (SMCs). Attempts to regenerate elastin in elastin-compromised blood vessels using tissue-engineering approaches is limited by the unavailability of elastogenic cues to upregulat...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.70

    authors: Kothapalli CR,Ramamurthi A

    更新日期:2008-03-01 00:00:00

  • Comparison of advanced therapy medicinal product gingiva and skin substitutes and their in vitro wound healing potentials.

    abstract::Skin and oral mucosa substitutes are a therapeutic option for closing hard-to-heal skin and oral wounds. Our aim was to develop bi-layered skin and gingiva substitutes, from 3 mm diameter biopsies, cultured under identical conditions, which are compliant with current European regulations for advanced therapy medicinal...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2438

    authors: Boink MA,Roffel S,Breetveld M,Thon M,Haasjes MSP,Waaijman T,Scheper RJ,Blok CS,Gibbs S

    更新日期:2018-02-01 00:00:00

  • Transbuccal platform for delivery of lipogenic actives to facial skin: Because fat matters.

    abstract::The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type:

    doi:10.1002/term.3087

    authors: Bojanowski K,Ma S,Applebaum R,Zhao H

    更新日期:2020-08-01 00:00:00

  • Optimization of culture conditions for osteogenically-induced mesenchymal stem cells in β-tricalcium phosphate ceramics with large interconnected channels.

    abstract::The aim of this study was to optimize culture conditions for human mesenchymal stem cells (hMSCs) in β-tricalcium phosphate ceramics with large interconnected channels. Fully interconnected macrochannels comprising pore diameters of 750 µm and 1400 µm were inserted into microporous β-tricalcium phosphate (β-TCP) scaff...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.331

    authors: Bernhardt A,Lode A,Peters F,Gelinsky M

    更新日期:2011-06-01 00:00:00

  • Hybrid PGS-PCL microfibrous scaffolds with improved mechanical and biological properties.

    abstract::Poly(glycerol sebacate) (PGS) is a biodegradable elastomer that has generated great interest as a scaffold material due to its desirable mechanical properties. However, the use of PGS in tissue engineering is limited by difficulties in casting micro- and nanofibrous structures, due to high temperatures and vacuum requ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.313

    authors: Sant S,Hwang CM,Lee SH,Khademhosseini A

    更新日期:2011-04-01 00:00:00

  • Rapid vascularization of starch-poly(caprolactone) in vivo by outgrowth endothelial cells in co-culture with primary osteoblasts.

    abstract::The successful integration of in vitro-generated tissues is dependent on adequate vascularization in vivo. Human outgrowth endothelial cells (OECs) isolated from the mononuclear cell fraction of peripheral blood represent a potent population of circulating endothelial progenitors that could provide a cell source for r...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.373

    authors: Ghanaati S,Fuchs S,Webber MJ,Orth C,Barbeck M,Gomes ME,Reis RL,Kirkpatrick CJ

    更新日期:2011-06-01 00:00:00

  • In vivo biocompatibility assessment of poly (ether imide) electrospun scaffolds.

    abstract::Poly(ether imide) (PEI), which can be chemically functionalized with biologically active ligands, has emerged as a potential biomaterial for medical implants. Electrospun PEI scaffolds have shown advantageous properties, such as enhanced endothelial cell adherence, proliferation and low platelet adhesion in in vitro e...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2002

    authors: Haase T,Krost A,Sauter T,Kratz K,Peter J,Kamann S,Jung F,Lendlein A,Zohlnhöfer D,Rüder C

    更新日期:2017-04-01 00:00:00

  • Novel method for the isolation of adipose stem cells (ASCs).

    abstract::Adipose stem cells (ASCs) represent a cell population with great potential for tissue engineering applications. Several articles have been published showing the proliferation and differentiation potential, the markers and the wide range of potential applications of these cells. In the majority of these studies the ASC...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.141

    authors: Rada T,Reis RL,Gomes ME

    更新日期:2009-02-01 00:00:00

  • Three-dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells.

    abstract::To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these cultures, the effects of oxygen concentration and co-cultures with s...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.295

    authors: Miyoshi H,Murao M,Ohshima N,Tun T

    更新日期:2011-02-01 00:00:00

  • Numerical assessment on the effective mechanical stimuli for matrix-associated metabolism in chondrocyte-seeded constructs.

    abstract::The self-regeneration capacity of articular cartilage is limited, due to its avascular and aneural nature. Loaded explants and cell cultures demonstrated that chondrocyte metabolism can be regulated via physiologic loading. However, the explicit ranges of mechanical stimuli that correspond to favourable metabolic resp...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.307

    authors: Tasci A,Ferguson SJ,Büchler P

    更新日期:2011-03-01 00:00:00

  • 3D ingrowth of bovine articular chondrocytes in biodegradable cryogel scaffolds for cartilage tissue engineering.

    abstract::A feasibility study was undertaken to examine the potential of biodegradable HEMA-lactate-dextran (HEMA-LLA-D)-based cryogels as scaffolds for cartilage tissue engineering. This was a preliminary in vitro study giving essential information on the biocompatibility of cryogels with cartilage cells. HEMA-lactate (HEMA-LL...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.375

    authors: Bölgen N,Yang Y,Korkusuz P,Güzel E,El Haj AJ,Pişkin E

    更新日期:2011-11-01 00:00:00

  • Micropatterned three-dimensional hydrogel system to study human endothelial-mesenchymal stem cell interactions.

    abstract::The creation of vascularized engineered tissues of clinically relevant size is a major challenge of tissue engineering. While it is known that endothelial and mural vascular cells are integral to the formation of stable blood vessels, the specific cell types and optimal conditions for engineered vascular networks are ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.231

    authors: Trkov S,Eng G,Di Liddo R,Parnigotto PP,Vunjak-Novakovic G

    更新日期:2010-03-01 00:00:00