Abstract：
PURPOSE:The aim of this study was to investigate the effects of quercetin on vascular endothelial growth factor (VEGF)-induced choroidal and retinal angiogenesis in vitro using a rhesus macaque choroid-retinal endothelial (RF/6A) cell line. METHODS:RF/6A cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. Then the cells were treated with different concentrations (from 0 to 100 μM) of quercetin and 100 ng/ml VEGF. The cell proliferation was assessed using cholecystokinin octapeptide dye. The cell migration was investigated by a Transwell assay. The tube formation was measured on Matrigel. Furthermore, the impact of quercetin's effects on VEGF-induced activation of VEGF receptor 2 (VEGFR-2) downstream signal pathways was tested by Western blot analysis. RESULTS:Quercetin inhibits RF/6A cell proliferation in a dose-dependent fashion: 22.7, 31.5 and 36.7% inhibition on treatment with 10, 50 and 100 μM quercetin, respectively. VEGF-induced migration and tube formation of RF/6A cells were also significantly inhibited by quercetin in a dose-dependent manner. Quercetin inhibits VEGF-induced VEGFR-2 downstream signal pathways of RF/6A. CONCLUSIONS:The results show that quercetin inhibits VEGF-induced cell proliferation, migration and tube formation of RF/6A. We suggest that quercetin inhibits VEGF-induced choroidal and retinal angiogenesis in vitro. Collectively, the findings in the present study suggest that quercetin inhibits VEGF-induced choroidal and retinal angiogenesis by targeting the VEGFR-2 pathway. This suggests that quercetin is a choroidal and retinal angiogenesis inhibitor.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Li F,Bai Y,Zhao M,Huang L,Li S,Li X,Chen Ydoi
10.1159/000369824subject
Has Abstractpub_date
2015-01-01 00:00:00pages
109-16issue
3eissn
0030-3747issn
1423-0259pii
000369824journal_volume
53pub_type
杂志文章abstract：:Lipid peroxidation was induced with porcine retinal homogenates using ferric iron or UV light. A number of lipid and aqueous-soluble antioxidants were tested and their protective effects measured after 60 min of exposure. The iron system produced up to 5 times more lipid hydroperoxides as quantified by the fluorescenc...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267103
更新日期：1991-01-01 00:00:00
abstract：PURPOSE:To examine melanocortin receptor (from MC-1 to MC-5) mRNA and the effect of alpha-melanocyte-stimulating hormone (alpha-MSH) on interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) expression in a human retinal pigment epithelial cell line (ARPE-19) stimulated with IL-1beta or tumor necrosis factor a...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000087699
更新日期：2005-09-01 00:00:00
abstract：:The presence of chondroitin- and heparan sulfate-containing proteoglycans and type IV collagen was searched for in human cameral mucous gel (CMG). Monoclonal antibodies specific for human heparan sulfate, chondroitin sulfate and collagen IV were used in ultrathin sections of Lowicryl-embedded human CMG. Only chondroit...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267569
更新日期：1995-01-01 00:00:00
abstract：:The refractive indices in various rat retinal layers, based on the total dry mass concentration, ranged from 1.369 to 1.385. The ratios of the refractive indices of neighboring retinal layers were near unity. This finding suggests that light passes through the rat retina without much refraction. ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267223
更新日期：1993-01-01 00:00:00
abstract：OBJECTIVES:To evaluate the efficacy of postenucleation saline wash and the effect of different antimicrobial agents on the microbial contamination of donor eyes. METHODS:Two hundred donor eyes were given saline wash and treated with any one of the following 5 randomly selected antimicrobial solutions: 1% povidone-iodi...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000095772
更新日期：2006-01-01 00:00:00
abstract：:In order to investigate the combined influence of age and light, the b-wave and oscillatory potentials (OPs) of the electroretinogram (ERG) were recorded in 1.5-, 7- and 12-month-old WAG/Rij rats, reared under homogenous low-intensity cyclic light exposure. Wistar albino rats of the same ages, reared under the same co...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267282
更新日期：1993-01-01 00:00:00
abstract：:To ascertain the effectiveness of topical antioxidant therapy on acute corneal inflammation, we have studied the effectiveness of topical treatment with a saline solution and with antioxidants such as 0.2% superoxide dismutase and 0.5% dimethylthiourea (DMTU) in a controlled experimental study. The evolution of the in...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267648
更新日期：1995-01-01 00:00:00
abstract：:Using cuprolinic blue staining, we histochemically examined the ultrastructural localization of proteoglycans in the fibrous-type human posterior capsule opacification. This capsule opacification contained proteoglycans that showed positive staining with cuprolinic blue. Many cuprolinic-blue-positive filaments were pr...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267707
更新日期：1995-01-01 00:00:00
abstract：:Rat lenses with experimentally induced cataract (either by naphthalene or by streptozotocin) were analyzed biochemically. Both noxae had some effects in common. Water-soluble protein and aldose reductase activity decreased, and glucose-6-phosphate dehydrogenase, phosphofructokinase and glutathione reductase activity i...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000266581
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:To evaluate a technique of autologous internal limiting membrane (ILM) fragment transplantation for the treatment of large, chronic, and/or refractory macular holes (MH). DESIGN:This was a 6-month prospective interventional case series. METHOD:Ten eyes of 10 patients with MH underwent pars plana vitretomy (...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000440767
更新日期：2015-01-01 00:00:00
abstract：:Epinephrine (1, 3 and 10 micrograms/kg) was found to increase retinal and choroidal blood flow (22, 49 and 67%) when it was administered intravenously. The increase in retinal and choroidal blood flow coincided well with the increase in systemic blood pressure (44, 65 and 96%, respectively). When epinephrine (2%) was ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000266728
更新日期：1988-01-01 00:00:00
abstract：:Vitamin E (d-alpha-tocopherol) and aspirin were evaluated for their effects on the phase separation temperature, Tc, of calf lens cytoplasm. Vitamin E and aspirin were added to nuclear homogenate and the change in Tc was measured. Aspirin had no measurable effect on Tc. Vitamin E lowered Tc at a rate of -22 degrees C ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265475
更新日期：1987-01-01 00:00:00
abstract：:We measured prostaglandin E2 (PGE2) and prostacyclin levels in the vitreous bodies of 5 eyes of patients with diabetic retinopathy complicated by rhegmatogenous retinal detachment (RRD) and compared them with the corresponding levels in 9 nondiabetic eyes with the same condition as well as with 10 control eyes of dece...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000266995
更新日期：1990-01-01 00:00:00
abstract：:Diabetic macular edema is considered the most important factor related to visual impairment in patients with diabetic retinopathy. Together with the use of grid and focal laser photocoagulation, today the intravitreal administration of pharmacotherapies represents the standard of care for the treatment of this complic...
journal_title：Ophthalmic research
pub_type： 杂志文章,评审
doi：10.1159/000362764
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of C constant for ray tracing-assisted intraocular lens (IOL) power calculation in the patients with different refractive power, we compared the refractive outcome of ray-tracing method based on C constant and conventional IOL calculation formula. METHODS:215 eyes which underwent phaco...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000507963
更新日期：2020-04-20 00:00:00
abstract：:The aqueous humors of 100 freshly enucleated rabbit eyes were assessed within 10 min postmortem by taking samples in the last stage of corneal dissection following the Dikstein-Maurice procedure. Aqueous levels of partial pressure of carbon dioxide (pCO2) averaged 58.2 +/- 8.5 mm Hg to give calculated tCO2 and bicarbo...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267269
更新日期：1993-01-01 00:00:00
abstract：INTRODUCTION:Retinitis pigmentosa (RP) is an inherited retinal disorder, characterized by photoreceptor degeneration inducing progressive vision loss. This study evaluates its impact on quality of life (QOL) and emotional states of patients affected by RP. METHODS:A cross-sectional study was conducted on 60 RP patient...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000435886
更新日期：2015-01-01 00:00:00
abstract：PURPOSE:Primary open-angle glaucoma (POAG) is a chronic progressive optic neuropathy, leading to degeneration of retinal ganglion cells and characteristic morphological changes at the optic disc. In advanced stages of the disease, functional tests, such as standard automated perimetry (SAP), are the main diagnostic too...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000501693
更新日期：2020-01-01 00:00:00
abstract：:The effect of topical neutral formaldehyde on the blood-aqueous barrier of the rat was studied. Thirty minutes after irritation, the protein concentration of the aqueous humour had increased from the control value of 1.95 +/- 0.25 to 2.95 +/- 0.45 mg/ml (p less than 0.05). Extravasation of Evans blue dye from the iris...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265442
更新日期：1986-01-01 00:00:00
abstract：PURPOSE:Abnormalities in electroretinograms (ERG), such as reduced amplitudes and delayed implicit times of a- and b-wave and oscillatory potentials (OPs), are one of the earliest features of diabetic retinopathy prior to obvious vascular changes in diabetic retinas. We have previously shown that serum levels of advanc...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000440768
更新日期：2015-01-01 00:00:00
abstract：AIMS:Experimental retinal research has gained great importance due to the ophthalmic pharmacotherapy era. An increasing number of drugs are constantly released into the market for the treatment of retinal diseases. In this review, animal species, animal models and toxicity assays in retinal research are discussed. MET...
journal_title：Ophthalmic research
pub_type： 杂志文章,评审
doi：10.1159/000312817
更新日期：2010-01-01 00:00:00
abstract：:The possible multiple effect of galactose diet and X-irradiation on cataract development in mice was investigated by scanning electron microscopy. X-ray cataracts were induced by maintaining animals either on a normal or 50% galactose diet. No detectable changes in lens morphology were seen in animals fed galactose al...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265280
更新日期：1983-01-01 00:00:00
abstract：PURPOSE:To describe the characteristics of macular lesions after eye trauma using spectral-domain optical coherence tomography (OCT). METHODS:We retrospectively reviewed and described 2-dimensional (2D) and 3-dimensional (3D) spectral-domain OCT characteristics of 24 consecutive eyes of 24 cases that were identified w...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000325732
更新日期：2012-01-01 00:00:00
abstract：:Modulation sensitivity functions ( MSFs ) of retinal ganglion cells of the pigeon were determined by recording action potentials from their end branching in the optic tectum as response indicator. The inverse Fourier transformation of a MSF is taken to represent the receptive field sensitivity function approximating t...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265299
更新日期：1984-01-01 00:00:00
abstract：PURPOSE:To assess the natural variability in the diameter of small collagen fibrils in the lamellae of corneal stroma of healthy young rabbits. MATERIALS AND METHODS:The corneas of 6 young adult female New Zealand White rabbits (2.1 kg) were prepared for transmission electron microscopy (TEM) by fixation with a cacody...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000096228
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:To evaluate the long-term outcome of trabeculectomy with intra- and postoperative 5-fluorouracil (5-FU) application in glaucoma. METHODS:Eighty-six patients with glaucoma planned for primary trabeculectomy with 5-FU and a minimum follow-up of 3 years were retrospectively analyzed. Success rates, postsurgical 5...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000464446
更新日期：2017-01-01 00:00:00
abstract：:Cannabinoid receptor involvement in the intraocular-pressure (IOP)-lowering effect of the cannabinoids has not been determined. These receptors bind four structural classes of agonists: the classical and the nonclassical cannabinoids, the aminoalkylindoles and the arachidonylethanolamides (the endogenous ligands). We ...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267984
更新日期：1997-01-01 00:00:00
abstract：:The seasonal variation in the incidence of retinal detachment over the period from 1981 to 1987 inclusive was investigated. Highly significant associations were observed between this variation and both sunshine and temperature. Hence, it is suggested that heat or cold may be important additional factors in the develop...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267026
更新日期：1990-01-01 00:00:00
abstract：:The binding of fluorescein and 6-carboxyfluorescein by normal, diabetic and in vitro glycosylated human serum proteins was analyzed by absorption and fluorescence spectroscopy, gel filtration and equilibrium dialysis. The absorption spectra of bound fluorochromes showed red shifts and hypochromic changes, and fluoroch...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000265219
更新日期：1982-01-01 00:00:00
abstract：:We examined 304 conjunctiva in 152 subjects by clinical, cytologic, and bacteriologic methods. Bacteriologic samples were evaluated after a 48-hour incubation. Bacterial growth was found in 8 (20.5%) of 39 patients with clinically normal conjunctiva and no polymorphonuclear leukocytes (PMNs), and in 8 (61.5%) of 13 su...
journal_title：Ophthalmic research
pub_type： 杂志文章
doi：10.1159/000267086
更新日期：1991-01-01 00:00:00
