Abstract：
:To create functional tissues having complex structures, bottom-up approaches to assembling small tissue modules into larger constructs have been emerging. Most of these approaches are based on chemical reactions or physical interactions at the interface between tissue modules. Here we report a biological assembly approach to integrate small tissue modules through endothelial capillary network formation. When adjacent tissue modules contain appropriate extracellular matrix materials and cell types that support robust endothelial capillary network formation, capillary tubules form and grow across the interface, resulting in assembly of the modules into a single, larger construct. It was shown that capillary networks formed in modules of dense fibrin gels seeded with human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs); adjacent modules were firmly assembled into an integrated construct having a strain to failure of 117 ± 26%, a tensile strength of 2208 ± 83 Pa and a Young's modulus of 2548 ± 574 Pa. Under the same culture conditions, capillary networks were absent in modules of dense fibrin gels seeded with either HUVECs or MSCs alone; adjacent modules disconnected even when handled gently. This biological assembly approach eliminates the need for chemical reactions or physical interactions and their associated limitations. In addition, the integrated constructs are prevascularized, and therefore this bottom-up assembly approach may also help address the issue of vascularization, another key challenge in tissue engineering.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Riesberg JJ,Shen Wdoi
10.1002/term.2008subject
Has Abstractpub_date
2015-09-01 00:00:00pages
1084-7issue
9eissn
1932-6254issn
1932-7005journal_volume
9pub_type
杂志文章abstract：:Bone marrow (BM) multipotent mesenchymal stromal cells (MSCs) present with multipotent differentiation potential and immunomodulatory properties. As an alternative to bone marrow, we have examined fetal membranes, amnion and chorion, of term human placenta as a potential source of multipotent MSCs. Here we show that a...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.40
更新日期：2007-07-01 00:00:00
abstract：:Advanced frontal sinus disease non-responsive to conservative therapy has been treated with fat obliteration for decades. More recently, a wide variety of autogenous, allogenic or synthetic materials have also been used. In this study we present a treatment based on totally autogenous procedures and materials that was...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.534
更新日期：2013-06-01 00:00:00
abstract：:Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bo...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3124
更新日期：2020-11-01 00:00:00
abstract：:Human bone marrow-derived mesenchymal stem cells (MSCs) have limited growth potential in vitro and cease to divide due to replicative senescence, which from a tissue-engineering perspective has practical implications, such as defining the correct starting points for differentiation and transplantation. Time spent in c...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1734
更新日期：2016-02-01 00:00:00
abstract：:A feasibility study was undertaken to examine the potential of biodegradable HEMA-lactate-dextran (HEMA-LLA-D)-based cryogels as scaffolds for cartilage tissue engineering. This was a preliminary in vitro study giving essential information on the biocompatibility of cryogels with cartilage cells. HEMA-lactate (HEMA-LL...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.375
更新日期：2011-11-01 00:00:00
abstract：:In this communication, we introduce boron nitride nanotube (BNNT)-functionalised muscle cell/microfibre mesh constructs, obtained via tissue engineering, as a three-dimensional (3D) platform to study a wireless stimulation system for electrically responsive cells and tissues. Our stimulation strategy exploits the piez...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1878
更新日期：2015-07-01 00:00:00
abstract：:Tissue engineering applications require an appropriate combination of a cell population, biochemical factors and scaffold materials. In this field, mesenchymal stem cells (MSCs) emerge as an attractive cell population, due to their ready availability and their potential to be differentiated into various mesodermal cel...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.545
更新日期：2013-07-01 00:00:00
abstract：:Biomaterials capable of controlling the release of multiple growth factors (GFs) could potentially promote the integration of co-transplanted neural progenitor cells (NPCs) and stimulate the plasticity and regenerability of the lesioned spinal cord. As a first step towards the employment of such a vehicle for cell the...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1797
更新日期：2015-08-01 00:00:00
abstract：:Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have potential clinical utility in the treatment of a multitude of ailments and diseases, due to their relative ease of isolation from patients and their capacity to form many cell types. However, hBM-MSCs are sparse, and can only be isolated in very small qu...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1515
更新日期：2014-03-01 00:00:00
abstract：:This study aims to investigate the superiorities of fast degrading elastomeric poly(glycerol sebacate) (PGS)/polycaprolactone (PCL) grafts over autologous vein grafts in the reconstruction of carotid artery, thus providing more suitable vascular grafts for carotid artery replacement. We fabricated small arterial graft...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2856
更新日期：2019-07-01 00:00:00
abstract：:Intervertebral disc (IVD) degeneration has been described as an aberrant, cell-mediated, age- and genetics-dependent molecular degeneration process, which can be accelerated by nutritional, mechanical and toxic factors. Collective involvement of these factors can result in structural failures, which are often associat...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.1583
更新日期：2014-12-01 00:00:00
abstract：:The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type：
doi：10.1002/term.3087
更新日期：2020-08-01 00:00:00
abstract：:Despite the high prevalence of skin conditions in the horse, there is a dearth of literature on the culture and biology of equine skin cells, and this is partially attributable to the lack of suitable in vitro skin models. The objective of this study was to develop a three-dimensional (3D) culture system that would su...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1788
更新日期：2016-08-01 00:00:00
abstract：:Islet replacement is a promising strategy for the treatment of patients with type 1 diabetes and patients who have undergone total pancreatectomy. Recent progress in cellular reprogramming technology may allow the transplantation of a patient's own pancreatic cells. Although many studies have reported the differentiat...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2228
更新日期：2017-11-01 00:00:00
abstract：:Recellularization of ex vivo-derived scaffolds remains a significant hurdle primarily due to the scaffolds subcellular pore size that restricts initial cell seeding to the scaffolds periphery and inhibits migration over time. With the aim to improve cell migration, repopulation, and graft mechanics, the effects of a f...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3015
更新日期：2020-03-01 00:00:00
abstract：:Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). This study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwe...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2534
更新日期：2018-02-01 00:00:00
abstract：:Obtaining an efficient, uniform and reproducible cell seeding of porous tubular scaffolds constitutes a major challenge for the successful development of tissue-engineered vascular grafts. In this study, a novel automated cell-seeding device utilizing direct cell deposition, patterning techniques and scaffold rotation...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.476
更新日期：2012-10-01 00:00:00
abstract：:Transplantation of bone marrow mesenchymal stem cells (BM-MSCs) has been shown to ameliorate the injured central nervous system (CNS). Although these effects were initially attributed to the putative differentiation of MSCs towards the neural lineage, it is now known that most of them are mediated by the secretome. Up...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.365
更新日期：2011-08-01 00:00:00
abstract：:The mechanism for stem cell-mediated improvement following acute myocardial infarction has been actively debated. We support hypotheses that the stem cell effect is primarily paracrine factor-linked. We used a heparin-presenting injectable nanofibre network to bind and deliver paracrine factors derived from hypoxic co...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.273
更新日期：2010-12-01 00:00:00
abstract：:Tissue engineering holds great promise to develop functional constructs resembling the structural organization of native tissues to improve or replace biological functions, with the ultimate goal of avoiding organ transplantation. In tissue engineering, cells are often seeded into artificial structures capable of supp...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.1978
更新日期：2016-09-01 00:00:00
abstract：:Regenerative medicine aims to replace lost cells and to restore damaged tissues and organs by either tissue-engineering approaches or stimulation of endogenous processes. Due to their biological properties, stem cells promise to be an effective source for such strategies. Especially adult multipotent stem cells (ASCs)...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.1923
更新日期：2016-01-01 00:00:00
abstract：:New injectable acrylic formulations have been prepared to be applied in restoration processes for intervertebral disks (IVDs). The solid phase of the formulations is composed of poly(methyl methacrylate) (PMMA), incorporating in some cases chondroitin sulphate (CS) as a regenerative bioactive molecule, whereas the liq...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.10
更新日期：2007-03-01 00:00:00
abstract：:Whole body vibration (WBV) is a very widespread mechanical stimulus used in physical therapy, rehabilitation and fitness centres. It has been demonstrated that vibration induces improvements in muscular strength and performance and increases bone density. We investigated the effects of low-amplitude, high frequency vi...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1533
更新日期：2014-05-01 00:00:00
abstract：:Previous researches have emphasized on suitability of decellularized tissues for regenerative applications. The decellularization of cartilage tissue has always been a challenge as the final product must be balanced in both immunogenic residue and mechanical properties. This study was designed to compare and optimize ...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2936
更新日期：2019-10-01 00:00:00
abstract：:Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only aesthetic but also functional problems with speech, eating, and breathing. Bone grafts using autologous cancellous...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2811
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:According to experimental studies, mesh implants may reduce in size up to 50.8% during their integration into soft tissues. This results in impaired mobility of the anterior abdominal wall and hernia recurrences, as well as affects patients' quality of life. Due to unsatisfactory radiographic contrast of pol...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3029
更新日期：2020-05-01 00:00:00
abstract：:The creation of vascularized engineered tissues of clinically relevant size is a major challenge of tissue engineering. While it is known that endothelial and mural vascular cells are integral to the formation of stable blood vessels, the specific cell types and optimal conditions for engineered vascular networks are ...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.231
更新日期：2010-03-01 00:00:00
abstract：:Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues an...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2092
更新日期：2017-07-01 00:00:00
abstract：:Nucleus pulposus (NP) regeneration by the application of injectable cell-embedded hydrogels is an appealing approach for tissue engineering. We investigated a thermo-reversible hydrogel (TR-HG), based on a modified polysaccharide with a thermo-reversible polyamide [poly(N-isopropylacrylamide), pNIPAM], which is made t...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1667
更新日期：2015-12-01 00:00:00
abstract：:Our recent clinical studies have demonstrated that autologous implantation of human cultured periosteal (hCP) sheets in combination with porous hydroxylapatite (HAp) particles at the site of periodontal bone defects strikingly facilitates tissue regeneration. To better understand how the hCP sheet functions at the imp...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.156
更新日期：2009-03-01 00:00:00
