Abstract:
:Reversible protein phosphorylation, regulated by protein kinases and phosphatases, mediates a switch between protein activity and cellular pathways that contribute to a large number of cellular processes. The Mycobacterium tuberculosis genome encodes 11 Serine/Threonine kinases (STPKs) which show close homology to eukaryotic kinases. This study aimed to elucidate the phosphoproteomic landscape of a clinical isolate of M. tuberculosis. We performed a high throughput mass spectrometric analysis of proteins extracted from an early-logarithmic phase culture. Whole cell lysate proteins were processed using the filter-aided sample preparation method, followed by phosphopeptide enrichment of tryptic peptides by strong cation exchange (SCX) and Titanium dioxide (TiO2) chromatography. The MaxQuant quantitative proteomics software package was used for protein identification. Our analysis identified 414 serine/threonine/tyrosine phosphorylated sites, with a distribution of S/T/Y sites; 38% on serine, 59% on threonine and 3% on tyrosine; present on 303 unique peptides mapping to 214 M. tuberculosis proteins. Only 45 of the S/T/Y phosphorylated proteins identified in our study had been previously described in the laboratory strain H37Rv, confirming previous reports. The remaining 169 phosphorylated proteins were newly identified in this clinical M. tuberculosis Beijing strain. We identified 5 novel tyrosine phosphorylated proteins. These findings not only expand upon our current understanding of the protein phosphorylation network in clinical M. tuberculosis but the data set also further extends and complements previous knowledge regarding phosphorylated peptides and phosphorylation sites in M. tuberculosis.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Fortuin S,Tomazella GG,Nagaraj N,Sampson SL,Gey van Pittius NC,Soares NC,Wiker HG,de Souza GA,Warren RMdoi
10.3389/fmicb.2015.00006subject
Has Abstractpub_date
2015-02-10 00:00:00pages
6issn
1664-302Xjournal_volume
6pub_type
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journal_title:Frontiers in microbiology
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pub_type: 杂志文章
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doi:10.3389/fmicb.2018.01453
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更新日期:2018-12-04 00:00:00
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doi:10.3389/fmicb.2017.01261
更新日期:2017-07-07 00:00:00
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pub_type: 杂志文章,评审
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