Abstract：
UNLABELLED:The current study aimed to evaluate the efficacy of sorafenib-based combined therapy against hepatocellular carcinoma (HCC). HepG2 cells were exposed to sorafenib, irinotecan, and oxaliplatin and then subjected to MTT assay to determine chemosensitivity. Flow cytometry was used to examine cell cycle distribution and cell apoptosis. Levels of cleaved caspase-8, -3, and PARP were determined by Western blot. Real-time PCR and Western blot were used to determine p53 expression, respectively. The efficacy of combined therapy were verified in nude mice bearing HepG2 xenografts. HepG2 cells used in the current study were sensitive to sorafenib, irinotecan, and oxaliplatin. Sorafenib arrested cell cycle in S phase and the peak effect appeared at 30 h post treatment. Sorafenib exposure for 30 h followed by irinotecan exposure for 48 h synergistically induced cell apoptosis in HepG2 cells. On the other hand, sorafenib-oxaliplatin sequential exposure for the same time only acted an additive effect in soliciting cell apoptosis. Sorafenib and irinotecan sequential treatment significantly increased the levels of cleaved caspase-8, -3, and PARP in HepG2 cells. Sorafenib suppressed p53 expression at both mRNA and protein levels, which might contribute to cell cycle arrest and sensitize tumor cells to irinotecan. Sorafenib and irinotecan sequential therapy was obviously superior to monotherapy in suppressing the growth of HepG2 xenografts. Sorafenib-irinotecan sequential treatment augmented the efficacy of either drug used alone in soliciting HepG2 cells apoptosis in vitro and in suppressing the growth of HepG2 xenografts in vivo. KEYWORDS:hepatocellular carcinoma, irinotecan, sorafenib, synergistic effect.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Wang Z,Zhao Z,Wu T,Song L,Zhang Ydoi
10.4149/neo_2015_022subject
Has Abstractpub_date
2015-01-01 00:00:00pages
172-9issue
2eissn
0028-2685issn
1338-4317journal_volume
62pub_type
杂志文章相关文献
NEOPLASMA文献大全abstract：UNLABELLED:Expression patterns of proteins involved in serine and glycine metabolism, and correlations of these patterns with clinicopathologic factors in phyllodes tumor were investigated. Tissue microarrays were prepared from 203 phyllodes tumors (PT) and stained with antibodies specific for glycine decarboxylase (GL...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2014_069
更新日期：2014-01-01 00:00:00
abstract：UNLABELLED:BioBran, enzymatically modified arabinoxylan from rice bran was tested for its possible effects on in vitro maturation of human dendritic cells (DC). Immature DC (iDC) derived from plastic-adhered, IL-4 and GM-CSF treated peripheral monocytes (Mo) were further cultured with cytokine maturation mix 1 (CMM1; T...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2009_02_89
更新日期：2009-01-01 00:00:00
abstract：:In a series of 2038 paraproteinemic sera, 9 IgD paraproteins (0.44%) were found. Clinical diagnosis of all the 9 was multiple myeloma. One patient had an IgD-lambda plasmacytoma which developed into plasmocellular leukemia. Our series of IgD paraproteins consisted of 6 females and 3 males with a mean age of 58.5 years...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The cytostatic cytokine tumor necrosis factor-alpha (TNF-alpha) and proliferative hormone adrenomedullin (AM) are abundantly expressed in human tumors. However, little is known about mechanism (s) through which TNF-alpha and AM exert their regulatory effects, especially in the regulation of proliferative activity in m...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Bladder cancer (BCa) is a common carcinoma of the urinary tract which occurs in the bladder mucosa. In recent years, people have recognized that epigenetic changes such as DNA methylation play important roles in the development of BCa, but the specific mechanism is unclear. In this study we detected the methylation ra...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2020_200928N1035
更新日期：2021-01-14 00:00:00
abstract：:The study was aimed at the proper detection of surface and cytoplasmic clonal Ig light/heavy chains in the frame of multiparameter flow cytometry analysis of some B-cell malignancies. An exact direct evidence has been obtained that the leukemia cells following staining by antibodies to immunoglobulins will need to be ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The influence of two kinds of transplantable melanomas on the secretory function of peritoneal macrophages, and particularly the release of TNF-alpha has been studied. The results showed a statistically significant increase of protein content in the supernatants from 24 h cultured macrophages from melanoma-bearing ham...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Aprotinin, a wide range proteinase inhibitor, was given alone to tumor-bearing mice and life span and several tumor growth parameters were recordered. Aprotinin showed anti-tumor effects in Hepatoma 22 and Lewis lung carcinoma, remaining ineffective in Sarcoma 37, Leukemia L1210 and Ehrlich ascitic carcinoma. ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Nasopharyngeal carcinoma (NPC) is a rare malignancy in the Czech Republic and Slovakia, with the standardized incidence rate of < 1:100000 person-years. Viral status of NPC in these non-endemic Eastern European regions is currently unknown. In a retrospective study, we evaluated the presence of EBV and HPV in 62 NPC c...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2016_013
更新日期：2016-01-01 00:00:00
abstract：:The aim of this study was a contemporaneous measurement of plasma cells proliferative and apoptotic activity in patients examined at the time of multiple myeloma (MM) diagnosis before initiation of chemotherapy, focussed on the following aspects: determination of prognostic significance of plasma cell propidium iodide...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:A modified form of the leukocyte adherence inhibition (LAI) test has been used to follow cell-mediated immunity (CMI) in rats of the Lewis inbred strain (LW), F1 hybrids inbred strains LW x AVN with transplanted syngeneic tumors B77, MC-1, and the random-bred Sprague Dawley strain with spontaneous REF tumor. Periphera...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Transcription factor 21 (TCF21) has been identified as a candidate tumor suppressor at 6q23-q24 that is epigenetically inactivated in many types of human cancers. We recently found that TCF21 methylation level was significantly increased in clear cell renal cell carcinoma (ccRCC). The purpose of this study was to inve...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2012_076
更新日期：2012-01-01 00:00:00
abstract：:Inbred Wistar rats were fed aflatoxin B1 (AFB1) during a period of 15 weeks. Some of the rats were sacrificed successively at different time intervals, and the remaining animals died a natural death. Beginning with the day of AFB1 treatment termination multifocal hepatomas appeared on the 265th day in males and on the...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Two simple spectrophotometric assays have been employed for the measurement of dopa oxidase activity and ceruloplasmin polyphenol oxidase activity in the sera from normal hamsters and hamsters bearing melanotic melanoma. Both activities were found to be augmented in tumor animals, the dopa oxidase activity much more p...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:This study aimed to identify the oncogenes associated with lung cancer based on the mRNA and single nucleotide polymorphism (SNP) profile data. The mRNA expression profile data of GSE43458 (80 cancer and 30 normal samples) and SNP profile data of GSE33355 (61 pairs of lung cancer samples and control samples) were down...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2015_117
更新日期：2015-01-01 00:00:00
abstract：:Sentinel node biopsy becomes a standard diagnostic and therapeutic tool in breast cancer in certain indications, while in other indications its validity is still reviewed. The authors present their experience with this method. In the years 2000-2006 700 patients underwent surgery. 704 sentinel node biopsies were perfo...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:To explore how Tim-3 is expressed and how its expression influences invasion and metastasis of colorectal cancer (CRC) cells. A total of 188 CRC patients were prospectively collected for this study. Meanwhile, 135 normal controls were incorporated during the same period. Intestinal samples of the CRC radical cancerous...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2017_112
更新日期：2017-01-01 00:00:00
abstract：:The determination of patient's resistance to a particular drug contributes to more efficient therapeutical approach. The aim of this study was to evaluate if the responsiveness of Chronic Myeloid Leukemia (CML) patients to Imatinib therapy was predictable from WT1 gene expression in peripheral blood leukocytes. To exa...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2009_05_393
更新日期：2009-01-01 00:00:00
abstract：:Oxaliplatin is commonly used in managing malignancy, including colorectal cancer. While treatment often fails due to decreased drug sensitivity, the mechanisms involved are not clear. In this study, we investigate how exosomal miR-19b participates in oxaliplatin sensitivity and then prove that miR-19b down-regulates o...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_180306N155
更新日期：2019-01-15 00:00:00
abstract：:Due to problems with identification and an incomplete understanding on the gastrointestinal stromal tumors (GIST) before 2001, there has been a lack of comprehensive long-term population-based studies on GIST epidemiology at present date. We used data from the online registry of Czech and Slovak GIST patients (http://...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2009_05_459
更新日期：2009-01-01 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) are strongly associated with cancer biology. The objective of this study is to investigate the expression profile of lncRNAs in human papillary thyroid carcinoma (PTC), and to understand the biological role of lncRNAs and their involvement in PTC oncogenesis. The lncRNAs and messenger RN...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_180625N414
更新日期：2019-03-05 00:00:00
abstract：:Serial cytogenetic characteristics of four lymphoblastoid cell lines derived from the peripheral blood of four patients with acute leukemia are presented, including G and C banding techniques and the analysis of sister chromatid exchanges by means of FPG technique. One of these lines turned into predominantly diploid ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:CD73 is a membrane-bound enzyme that catalyzes the extracellular conversion of adenosine monophosphate to adenosine. Adenosine is thought to play a role in promoting tumor growth and survival together with suppressing the host immune responses, which contribute to the multistep process of tumorigenesis. Here, we studi...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2020_190826N822
更新日期：2020-07-01 00:00:00
abstract：:Relation between the electronic structure of aromatic compounds and their carcinogenic activity has been correlated. Hypotheses of K, L regions have been employed. Possibilities of better theoretical description of carcinogenic activity have been examined by including the effect of medium and by using a more exact qua...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The reliability of a particular in vitro parameter as potential prognostic biomarker of individual radiosensitivity is still discussed. Therefore, several in vitro radiation-induced cellular endpoints including initial, oxidative and residual DNA damage and the rate of DNA repair were assessed in peripheral blood lymp...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Metadherin (MTDH) is an oncoprotein and is expressed at high levels in a wide variety of human carcinomas, which represents an important genetic determinant and regulates multiple events in tumorigenesis. MTDH promotes breast cancer cell proliferation and tumorigenesis through the activation of numerous signaling path...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_170228N149
更新日期：2018-01-01 00:00:00
abstract：:Heat Shock proteins 70 (Hsp70) is a family of highly conserved molecules that maintain the function of crucial cellular pathways during stress. Hsp70 derived from tumor cells is bound with tumor antigenic peptides from the diverse antigen cytosolic pool. Tumor-derived Hsp70 preparations after ex vivo administration pe...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2009_03_259
更新日期：2009-01-01 00:00:00
abstract：:The lack of suitable criteria to predict the response to chemo- and or radiotherapy for individual patients with squamous cell carcinoma of the head and neck (HNSCC) remains still a major problem. This study was conducted to analyze prognostic significance of mitotic and apoptotic index and the DNA flow cytometric ana...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Loss of heterozygosity (LOH) has been shown to be an important prognostic factor in a variety of malignant neoplasm's. Cervical cancer develops as result of multiple genetic alterations. The aim of this study was to analyze presence of LOH in cervical cancer and to identify the correlation between LOH and survival and...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:We report on 12 patients who fulfilled the FAB diagnosis criteria of chronic myelomonocytic leukemia (CMML). Some clinical findings (splenomegaly) as well as biological parameters (WBC count, cellularity of bone marrow sections, presence of dyserythropoiesis in bone marrow smears) were subjected to particular analysis...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
