Abstract：
OBJECTIVE:To dissect the functioning mode of miR-645 on renal clear cell carcinoma cell metastasis and growth, and provide therapeutic targets for renal clear cell carcinoma. PATIENTS AND METHODS:Quantitative Real-time PCR (qRT-PCR) assay was employed to detect miR-645 expression level. Wound healing assay and transwell assay were performed to investigate metastasis capacity of renal clear cell carcinoma cells. Cell Counting Kit 8 (CCK8) assay was incorporated to assess cell proliferation capacity. Flow cytometry was used to identify cell apoptosis and cell cycle distribution. Protein levels were assessed by Western blotting assay. The target gene was predicted and verified by bioinformatics analysis and luciferase assay. RESULTS:MiR-645 was upregulated in renal clear cell carcinoma tissues when compared with para-carcinoma tissues (n=32). Downregulated miR-645 could attenuate cell migration and invasion capacities, as well as inhibited cell proliferation capacity, promoted cell apoptosis and cell cycle arrest at G0/G1 phase. GK5 was chosen as the target gene of miR-645 by bioinformatics analysis and luciferase reporter assay. Moreover, silence of GK5 could rescue tumor suppression role of downregulated miR-645 on renal clear cell carcinoma metastasis. CONCLUSIONS:Knockdown of miR-645 exerted tumor-suppressive effects on renal clear cell carcinoma metastasis and growth via targeting GK5 in vitro, which provided an innovative and candidate target for diagnose and treatment of renal clear cell carcinoma.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Chen J,Shu Y,Yu Q,Shen Wsubject
Has Abstractpub_date
2017-10-01 00:00:00pages
4557-4565issue
20eissn
1128-3602issn
2284-0729journal_volume
21pub_type
杂志文章abstract：:Vascular Endothelial Growth Factor (VEGF) plays a crucial role in the establishment of the vascular tree pattern. New vessels can be formed by two different ways; in the development of kidney both vasculogenesis and angiogenesis participate to microvessel assembly. VEGF and its receptor (VEGF-R) are co-expressed durin...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：1999-07-01 00:00:00
abstract：INTRODUCTION:The hysto-morfological composition of the ascending aorta wall gives to the vessel its characteristic elasticity/distensibility, which is deteriorated due to both physiological (age) and pathological events (hypertension, diabetes, dyslipidemia). This contributes to reduce the wall elasticity and to occurr...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:The cone-beam computerized tomography (CBCT) has become widely used for oral and maxillofacial imaging, providing a good spatial resolution, gray density range, and contrast, as well as a good pixel/noise ratio. In the CBCT the dimensional accuracy is also comparable with Computer Tomography (CT), but in con...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:Circular RNAs are a subgroup of non-coding RNAs and generated by a mammalian genome. The purpose of this study was to investigate the clinical significance, biological function and molecular mechanism of circ_0067934 in human non-small cell lung cancer (NSCLC). PATIENTS AND METHODS:We assayed expression leve...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201805_15063
更新日期：2018-05-01 00:00:00
abstract：OBJECTIVE:This study aims to detect the expression pattern of microRNA-375 in colorectal cancer (CRC), and to examine its specific mechanism in regulating the progression of CRC. PATIENTS AND METHODS:We detected microRNA-375 expression in 50 pairs of CRC and paracancerous tissues by quantitative real-time polymerase c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201906_18055
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:Evidences from cell culture experiments suggest a link between cholesterol and nervous system disease. Statins may have neurotoxic or neuroprotective effects, but these effects remain controversial. Therefore, the present study was aimed to investigate the possible toxicity of statins on a neurite outgrowth i...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVES:Aggressive cell growth and chemoresistance are notorious obstacles in neuroblastoma therapy. Accumulating evidence suggests that survivin is preferentially expressed in cancer cells and plays a crucial role in cell division and apoptosis dysfunction. Thus, in the present study, we investigated whether silenc...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:This study aimed to characterize the effect of Twist2 on epithelial-to-mesenchymal transition (EMT) and the invasive potential of colorectal cancer (CRC) cells and to explore the mechanisms underlying the regulative effect of Twist1 and Twist2 on matrix metalloproteinase 2 (MMP2) expression in CRC. PATIENTS ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201812_16514
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVE:We aimed at investigating the clinical efficacy of recombinant human basic fibroblast growth factor combined with compound polymyxin B ointment and the local application of insulin on wound healing of deep second-degree burn in diabetes mellitus. PATIENTS AND METHODS:80 diabetes mellitus patients complicated...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：10.26355/eurrev_201907_18345
更新日期：2019-07-01 00:00:00
abstract：OBJECTIVE:Pulmonary embolism (PE) is a primary clinical manifestation of venous thromboembolism (VTE). It has been demonstrated that pulmonary endothelial cells (PECs) are apoptotic-resistant in PE. MATERIALS AND METHODS:In this study, PECs were collected from PE patients and mouse models. Western blot, RT-PCR, flow c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202010_23420
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVE:Indoleamine 2,3-dioxygenase (IDO) initiated tryptophan degradation in the placenta has a role in the prevention of allogeneic fetus rejection by T-cells. The present study aimed to investigate the relationship between IDO and CD4+CD25+Foxp3+ T cells in pregnant mice. MATERIALS AND METHODS:The percentage of C...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：:This review illustrates some brief considerations of the medical use of cannabis recently issued in Italy. History and uses of cannabis throughout centuries and different countries are illustrated together with a description of botany and active phytocannabinoids. Then, medical use of cannabis anti-pain treatment for ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_201802_14405
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the inhibitory role of microRNA-1299 (miR-1299) in prostate cancer, and to explore the possible underlying mechanism. PATIENTS AND METHODS:The expression of miR-1299 in 35 PCa tissues and para-carcinoma tissues, as well as PCa cell lines (PC-3) and prostatic epithelia...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201901_16865
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:Pathogenesis factor of pregnant hypertension is still unclear and lacks of effective treatment. MiR-155 is a recently discovered miRNA molecule with differential expression in pregnant hypertension, which participates in the disease regulation. As a downstream target gene of miR-155, FOXO3a is correlated with...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201810_16133
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVE:Traumatic lung injury (TLI) can cause inflammation and oxidative stress, or even leads to acute respiratory distress syndrome (ARDS) and death. Nuclear factor erythroid-2 related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1 (Keap1)-antioxidant response element (ARE) signal pathway participates in disea...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201803_14486
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:Volatile halocarbon, bromobenzene (BB), is frequently encountered in table-ready foods as contaminants residues. The objective of this study was to investigate whether black seed oil could attenuate hepato-renal injury induced by BB exposure. MATERIALS AND METHODS:The evaluation was done through measuring li...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:The occurrence rate of delayed fracture healing in diabetes mellitus patients is high. Transforming growth factor β1 (TGF-β1) is an important regulatory factor in bone tissue repair and regeneration. However, TGF-β1 expression and its function in diabetic patient fracture have not been fully elucidated. PATI...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201801_16064
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The aim of this paper is to investigate epidemiological data (age, gender), sites, etiology and surgical approach of zygomatic fracture. MATERIALS AND METHODS:A 9 years retrospective clinical and epidemiologic study evaluated 642 patients treated for zygomatic fracture. There were 569 men and...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:To explore the effects of interventional therapy on human immunodeficiency virus (HIV)-1 Tat interactive protein 2/Tat interactive protein 30 (HTATIP2/TIP30), B7-H4 and short-term curative effect in primary hepatocellular carcinoma. PATIENTS AND METHODS:62 patients with primary hepatocellular carcinoma admit...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：10.26355/eurrev_201810_16144
更新日期：2018-10-01 00:00:00
abstract：:Allergic bronchopulmonary aspergillosis (ABPA) occurs in 7-9% of patients with cystic fibrosis (CF) and causes a worsening of lung function and respiratory symptoms. Standard treatment of ABPA consists of oral steroids; however, higher corticosteroid therapy associated to antifungal agent (itraconazole) long-term trea...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:In the last 25 years, obesity has reached epidemic levels of prevalence. It has even affected the children such that rates of severe childhood obesity that have almost tripled in numbers. These numbers are alarming because of the known fact that obesity is associated with an increased risk of several comorbidities as ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2015-05-01 00:00:00
abstract：:Inositol is a physiological compound belonging to the sugar family. The two inositol stereoisomers, myo-inositol and D-chiroinositol are the two main stereisomers present in our body. Myo-inositol is the precursor of inositol triphosphate, a second messenger regulating many hormones such as TSH, FSH and insulin. D-chi...
journal_title：European review for medical and pharmacological sciences
pub_type： 历史文章,杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:To investigate serum concentration of nesfatin-1 in underweight children who have poor appetite, and its association with anthropometric markers of malnutrition. PATIENTS AND METHODS:We recruited 50 underweight children and adolescents (aged 2-18 years) who presented with loss of appetite. Thirty age- and se...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-12-01 00:00:00
abstract：:The article "MiR-140-5p targets BCL2L1 to promote cardiomyocyte apoptosis, by M.-Y. Sun, L.-P. Li, published in Eur Rev Med Pharmacol Sci 2020; 24 (11): 6311-6322-DOI: 10.26355/eurrev_202006_21529-PMID: 32572928" has been withdrawn from the authors. They stated that "they are still working on this study" and that "the...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202101_24298
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Artocarpus heterophyllus (jackfruit) is a latex producing plant. Plant latex is produced from secretory cells and contains many intergradients. It also has been used in folk medicine. This study aimed to purify and characterize the biological activities of a protease from jackfruit latex. MATE...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:Recently, long noncoding RNAs (lncRNAs) have attracted much attention for their roles in tumor progression. The aim of this study was to investigate the exact role of lncRNA OR3A4 in the development of ovarian cancer (OC), and to explore the possible underlying mechanism. PATIENTS AND METHODS:OR3A4 expressio...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201903_17380
更新日期：2019-03-01 00:00:00
abstract：:Tamoxifen is a synthetic non steroidal anti-estrogen used to treat patients with breast cancer and healthy subjects with high risk of breast cancer. It was aimed to study the short term effects of tamoxifen on the plasma total antioxidant capacity (TAC), nitric oxide (NO) and the adenosine deaminase activity (ADA) in ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVE:We aimed to investigate whether miR-181d may be involved in steroid-induced osteonecrosis of the femoral head (ONFH) and its underlying mechanism. PATIENTS AND METHODS:Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-181d in bone marrow of 5 case...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201807_15393
更新日期：2018-07-01 00:00:00
abstract：:The last two decades have witnessed two large-scale pandemics caused by coronaviruses, including severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS). At the end of 2019, another novel coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hit Wuhan...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_202004_21046
更新日期：2020-04-01 00:00:00
abstract：OBJECTIVE:In this study, we investigated whether circulating microRNA-137 (miR-137) could be a potential biomarker for patients with glioblastoma (GBM). PATIENTS AND METHODS:Serum samples were collected from 64 GBM patients and 64 healthy controls. The expression level of circulating miR-137 was compared by quantitati...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
