Abstract:
:The cystine/glutamate exchanger (system xc (-)) mediates the transport of cystine into the cell in exchange for glutamate. By releasing glutamate, system xc (-) can potentially cause excitotoxicity. However, through providing cystine to the cell, it regulates the levels of cellular glutathione (GSH), the main endogenous intracellular antioxidant, and may protect cells against oxidative stress. We tested two different compounds that deplete primary cortical cultures containing both neurons and astrocytes of intracellular GSH, L-buthionine-sulfoximine (L-BSO), and diethyl maleate (DEM). Both compounds caused significant concentration and time dependent decreases in intracellular GSH levels. However; DEM caused an increase in radiolabeled cystine uptake through system xc (-), while unexpectedly BSO caused a decrease in uptake. The compounds caused similar low levels of neurotoxicity, while only BSO caused an increase in oxidative stress. The mechanism of GSH depletion by these two compounds is different, DEM directly conjugates to GSH, while BSO inhibits γ-glutamylcysteine synthetase, a key enzyme in GSH synthesis. As would be expected from these mechanisms of action, DEM caused a decrease in intracellular cysteine, while BSO increased cysteine levels. The results suggest that negative feedback by intracellular cysteine is an important regulator of system xc (-) in this culture system.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
Albano R,Raddatz NJ,Hjelmhaug J,Baker DA,Lobner Ddoi
10.1155/2015/269371subject
Has Abstractpub_date
2015-01-01 00:00:00pages
269371eissn
1942-0900issn
1942-0994journal_volume
2015pub_type
杂志文章abstract:Background:Infrasound is a major threat to global health by causing injuries of the central nervous system (CNS). However, there remains no effective therapeutic agent for preventing infrasound-caused CNS injury. 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glycoside (THSG) exerts protective function against CNS injuries and ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/6576718
更新日期:2020-01-06 00:00:00
abstract::The widely used herbicide atrazine (ATR) can cause many adverse effects including immunotoxicity, but the underlying mechanisms are not fully understood. The current study investigated the role of oxidative stress and calcium homeostasis in ATR-induced immunotoxicity in mice. ATR at doses of 0, 100, 200, or 400 mg/kg ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/7978219
更新日期:2016-01-01 00:00:00
abstract::As one of the major risks for urolithiasis, hyperoxaluria can be caused by genetic defect or dietary intake. And high oxalate induced renal epithelial cells injury is related to oxidative stress and mitochondrial dysfunction. Here, we investigated whether MitoTEMPO, a mitochondria-targeted antioxidant, could protect a...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/7528090
更新日期:2017-01-01 00:00:00
abstract::Although a growing body of evidence suggests that plant polyphenols can modulate human immune responses, their simultaneous action on monocyte and neutrophil oxidative burst is currently poorly understood. Based on the hypothesis that various polyphenols contained in plant extracts might affect the oxidative burst of ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2014/764367
更新日期:2014-01-01 00:00:00
abstract::Lipid metabolism disorder and inflammation are essential promoters in pathogenesis of liver injury in type 2 diabetes. Puerarin (PUR) has been reported to exert beneficial effects on many diabetic cardiovascular diseases and chemical-induced liver injuries, but its effects on diabetic liver injury and its mechanism ar...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/4545321
更新日期:2018-07-02 00:00:00
abstract:SCOPE:To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes. The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol met...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2015/480943
更新日期:2015-01-01 00:00:00
abstract::Autophagy is upregulated in spinal cord ischemia reperfusion (SCIR) injury; however, its expression mechanism is largely unknown; moreover, whether autophagy plays a neuroprotective or neurodegenerative role in SCIR injury remains controversial. To explore these issues, we created an SCIR injury rat model via aortic a...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/8640284
更新日期:2017-01-01 00:00:00
abstract::Hemolytic diseases are characterized by an accelerated breakdown of red blood cells (RBCs) and the release of hemoglobin (Hb). Following, RBC lysis Hb oxidation occurs with the formation of different redox states of Hb (metHb and ferrylHb) and the release of heme. ferrylHb is unstable and decomposes to metHb with the ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/8929020
更新日期:2020-04-25 00:00:00
abstract::The total lifespan of the yeast Saccharomyces cerevisiae may be divided into two phases: the reproductive phase, during which the cell undergoes mitosis cycles to produce successive buds, and the postreproductive phase, which extends from the last division to cell death. These phases may be regulated by a common mecha...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/1898421
更新日期:2018-03-20 00:00:00
abstract::Essential oils of some aromatic plants provide an effective nonmedicinal option to control liver fibrosis. Mentha piperita L. essential oil (MPEO) have been reported to possess protective effects against hepatotoxicity. However, its effect against liver fibrosis remains unknown. The present study investigated the anti...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/4039753
更新日期:2018-04-19 00:00:00
abstract::Viruses play an important role in cancerogenesis. It is estimated that approximately 20% of all cancers are linked to infectious agents. The viral genes modulate the physiological machinery of infected cells that lead to cell transformation and development of cancer. One of the important adoptive responses by the canc...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2016/6468342
更新日期:2016-01-01 00:00:00
abstract::Lung cancer is a leading cause of cancer death worldwide, and non-small-cell lung cancer (NSCLC) accounts for 85% of lung cancer, which is highly metastatic, leading to the poor survival rate of patients. We recently reported that 4-[4-(4-hydroxyphenoxy)phenoxy]phenol (4-HPPP), a phenoxyphenol, exerts antihepatoma eff...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/5167292
更新日期:2020-01-07 00:00:00
abstract:OBJECTIVE:To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury. METHODS:36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (n = 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2015/106836
更新日期:2015-01-01 00:00:00
abstract::Aging is a risk factor for cardiovascular disease, and there is no effective therapeutic approach to alleviate this condition. NF-κB and TNF-α have been implicated in the activation of the aging process, but the signaling molecules responsible for the inactivation of NF-κB and TNF-α remain unknown. Exosomes have been ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/9739258
更新日期:2019-04-08 00:00:00
abstract::The present study discusses about the effects of a combination of probiotics able to stimulate the immune system of patients affected by Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). To this purpose, patients diagnosed according to Fukuda's criteria and treated with probiotics were analyzed by means of ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/1684198
更新日期:2019-11-23 00:00:00
abstract::Hydrogen sulfide (H2S), a colorless gas smelling of rotten egg, has long been recognized as a toxic gas and environment pollutant. However, increasing evidence suggests that H2S acts as a novel gasotransmitter and plays important roles in a variety of physiological and pathological processes in mammals. H2S is involve...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2019/3831713
更新日期:2019-01-20 00:00:00
abstract::UVA irradiation induced ROS-mediated photo damage to the human skin leading to coarseness, wrinkling, pigmentation, and cutaneous malignancies. We investigated the dermatoprotective efficacies of submicromolar concentrations of ergothioneine (EGT, 0.125-0.5 μM), which occurs naturally as a sulfur-containing amino acid...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/2576823
更新日期:2020-02-12 00:00:00
abstract::[This corrects the article DOI: 10.1155/2017/4824371.]. ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 已发布勘误
doi:10.1155/2019/2029096
更新日期:2019-08-06 00:00:00
abstract::In Alzheimer's disease, lipid alterations point towards peroxisomal dysfunctions. Indeed, a cortical accumulation of saturated very long chain fatty acids (VLCFAs: C22:0, C24:0, C26:0), substrates for peroxisomal β-oxidation, has been found in Alzheimer patients. This study was realized to investigate the effects of V...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2012/623257
更新日期:2012-01-01 00:00:00
abstract::The neutrophil elastase inhibitor sivelestat (ONO-5046) possesses unknown mechanisms of cardioprotection when infused following global ischemia, even in the absence of neutrophils. Since myocardial ischemia-reperfusion injury is strongly associated with endothelial dysfunction and reactive oxygen species (ROS) generat...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2013/279847
更新日期:2013-01-01 00:00:00
abstract::Mitochondria are the primary site of cellular energy generation and reactive oxygen species (ROS) accumulation. Elevated ROS levels are detrimental to normal cell function and have been linked to the pathogenesis of neurodegenerative disorders such as Down's syndrome (DS) and Alzheimer's disease (AD). RCAN1 is abundan...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2014/520316
更新日期:2014-01-01 00:00:00
abstract:Background:Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The idea of therapeutic angiogenesis in ischemic myocardium is a promising strategy for MI patients. Buyang Huanwu decoction (BHD), a famous Chinese herbal prescription, exerted antioxidant, antiapoptotic, and ant...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/4275984
更新日期:2019-04-16 00:00:00
abstract::TRPM7 is a potential therapeutic target for treatment of prostate cancer. In this study, we investigated the effects of nonselective TRPM7 inhibitor carvacrol on cell proliferation, migration, and invasion of prostate cancer PC-3 and DU145 cells. Our results showed that carvacrol blocked TRPM7-like currents in PC-3 an...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/1469693
更新日期:2016-01-01 00:00:00
abstract::The aim of this study was to evaluate the involvement of TNF-α and insulin resistance (IR) in the inflammatory process, oxidative stress, and disease activity in patients with rheumatoid arthritis (RA). This cross-sectional study included 270 subjects (control group, n = 97) and RA patients (n = 173). RA patients were...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/8962763
更新日期:2016-01-01 00:00:00
abstract::Since excessive reactive oxygen species (ROS) is known to be associated with aging and age-related diseases, strategies modulating ROS level and antioxidant defense systems may contribute to the delay of senescence. Here we show that the protein hydrolyzate from Angelica sinensis was capable of increasing oxidative su...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/8956981
更新日期:2016-01-01 00:00:00
abstract::Several of the most prevalent etiological factors which contribute towards global death rates are associated with cardiovascular diseases (CVDs), which include a range of conditions such as angina, rheumatic heart disease, and venous thrombosis. Extensive research has been conducted into the role played by oxidative s...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2017/6504518
更新日期:2017-01-01 00:00:00
abstract::We conducted quantitative cellular respiration analysis on samples taken from human breast cancer (HBC) and human colorectal cancer (HCC) patients. Respiratory capacity is not lost as a result of tumor formation and even though, functionally, complex I in HCC was found to be suppressed, it was not evident on the prote...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/1372640
更新日期:2017-01-01 00:00:00
abstract::Ferroptosis is an iron-dependent type of cell death distinct from apoptosis or necrosis characterized by accumulation of reactive oxygen species. The combination of siramesine, a lysosomotropic agent, and lapatinib, a dual tyrosine kinase inhibitor (TKI), synergistically induced cell death in breast cancer cells media...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/9561281
更新日期:2019-09-17 00:00:00
abstract::Since both O-GlcNAcylation and autophagy sense intracellular nutrient level, the alteration of those two pathways plays substantial roles in the progression of heart failure. Hence, determining the relationship between O-GlcNAcylation and autophagy is imperative to understand, prevent, and treat heart failure. However...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/5602396
更新日期:2020-08-11 00:00:00
abstract::Ganoderma lucidum, mushroom used for centuries by Asian peoples as food supplement, has been shown interesting biological activities, including over the Central Nervous System. Besides, these mushroom bioactive compounds present antioxidant and anti-inflammatory activities. On the side, binge drinking paradigm consist...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/2497845
更新日期:2020-07-30 00:00:00