Abstract:
:There is a need for preclinical testing systems that predict the efficacy, safety and pharmacokinetics of cancer therapies better than existing in vitro and in vivo animal models. An approach to the development of predictive in vitro systems is to more closely recapitulate the cellular and spatial complexity of human cancers. One limitation of using current in vitro systems to model cancers is the lack of an appropriately large volume to accommodate the development of this complexity over time. To address this limitation, we have designed and constructed a novel flow-perfusion bioreactor system that can support large-volume, engineered tissue comprised of multicellular cancer surrogates by modifying current microfluidic devices. Key features of this technology are a three-dimensional (3D) volume (1.2 cm3 ) that has greater tissue thickness than is utilized in existing microfluidic systems and the ability to perfuse the volume, enabling the development of realistic tumour geometry. The constructs were fabricated by infiltrating porous carbon foams with an extracellular matrix (ECM) hydrogel and engineering through-microchannels. The carbon foam structurally supported the hydrogel and microchannel patency for up to 161 h. The ECM hydrogel was shown to adhere to the carbon foam and polydimethylsiloxane flow chamber, which housed the hydrogel-foam construct, when surfaces were coated with glutaraldehyde (carbon foam) and nitric acid (polydimethylsiloxane). Additionally, the viability of breast cancer cells and fibroblasts was higher in the presence of perfused microchannels in comparison to similar preparations without microchannels or perfusion. Therefore, the flow-perfusion bioreactor system supports cell viability in volume and stromal contexts that are physiologically-relevant. Copyright © 2015 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Marshall LE,Goliwas KF,Miller LM,Penman AD,Frost AR,Berry JLdoi
10.1002/term.2026subject
Has Abstractpub_date
2017-04-01 00:00:00pages
1242-1250issue
4eissn
1932-6254issn
1932-7005journal_volume
11pub_type
杂志文章abstract::The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair. A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprot...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2403
更新日期:2018-01-01 00:00:00
abstract::Tissue engineering applications require an appropriate combination of a cell population, biochemical factors and scaffold materials. In this field, mesenchymal stem cells (MSCs) emerge as an attractive cell population, due to their ready availability and their potential to be differentiated into various mesodermal cel...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.545
更新日期:2013-07-01 00:00:00
abstract::Poly(glycerol sebacate) (PGS) is a biodegradable elastomer that has generated great interest as a scaffold material due to its desirable mechanical properties. However, the use of PGS in tissue engineering is limited by difficulties in casting micro- and nanofibrous structures, due to high temperatures and vacuum requ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.313
更新日期:2011-04-01 00:00:00
abstract::Prevascularization is essential to ensure the viability, functionality, and successful integration of tissue-engineered three-dimensional (3D) constructs with surrounding host tissues after transplantation. Human mesenchymal stem cell (hMSC) sheet can be prevascularized by coculturing with endothelial cells (ECs), and...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3167
更新日期:2021-01-11 00:00:00
abstract::In order to examine the differentiation potential of the tenocytes expanded in our defined culture medium (reported previously) and the effect of sequential combination of the two culture conditions on human tenocytes, a two-dimensional and three-dimensional experimental approach was used. Human tenocytes were sequent...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1791
更新日期:2016-03-01 00:00:00
abstract::This study investigates whether platelet-rich plasma (PRP) is an activator of tendon-derived stem cells (TDSCs) to promote regeneration of Achilles tendon post-rupture in rats. In the in vitro study, PRGF (activated PRP) significantly enhanced cell DNA synthesis, improved viability and promoted proliferation, while fa...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2020
更新日期:2017-04-01 00:00:00
abstract::Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bo...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3124
更新日期:2020-11-01 00:00:00
abstract::The objective was to improve proliferation and chondrogenic potential of adipose stem cells (ASCs) by expansion on extracellular matrix (ECM) deposited by either ASCs or synovium-derived stem cells (SDSCs). ASCs isolated from porcine infrapatellar fat pad were separately expanded on conventional plastic flasks, ASC-de...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.505
更新日期:2013-01-01 00:00:00
abstract::The composition and organization of the vocal fold extracellular matrix (ECM) provide the viscoelastic mechanical properties that are required to sustain high-frequency vibration during voice production. Although vocal injury and pathology are known to produce alterations in matrix physiology, the mechanisms responsib...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.219
更新日期:2010-01-01 00:00:00
abstract::The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1848
更新日期:2015-08-01 00:00:00
abstract::In clinical cases, many orthopaedists have been troubled with bone fragility, such as fractures after devitalization therapy for bone tumour, pathological fractures and metastatic tumours. The aim of this study was to determine whether loss of osteogenic capacity of devitalized autologous bones can be rescued using cu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.67
更新日期:2008-01-01 00:00:00
abstract::The aim of this study was to optimize culture conditions for human mesenchymal stem cells (hMSCs) in β-tricalcium phosphate ceramics with large interconnected channels. Fully interconnected macrochannels comprising pore diameters of 750 µm and 1400 µm were inserted into microporous β-tricalcium phosphate (β-TCP) scaff...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.331
更新日期:2011-06-01 00:00:00
abstract::The mechanism for stem cell-mediated improvement following acute myocardial infarction has been actively debated. We support hypotheses that the stem cell effect is primarily paracrine factor-linked. We used a heparin-presenting injectable nanofibre network to bind and deliver paracrine factors derived from hypoxic co...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.273
更新日期:2010-12-01 00:00:00
abstract::Unique poly(l-lactic acid) (PLLA)-based scaffolds were constructed by embedding knitted PLLA yarns within a bioresorbable and differentially crosslinked three-dimensional (3D) oxidized collagen scaffold. The scaffolds were designed specifically for the repair of complex incisional abdominal wall hernias and the repair...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1837
更新日期:2016-10-01 00:00:00
abstract::Nonunion treatment has a high rate of success, although recalcitrant nonunion may determine the need for amputation. Therefore, new treatment options are continuously investigated in order to further reduce the risk of nonunion recurrence. This study aimed to (a) develop a new large animal model for bone atrophic nonu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2732
更新日期:2018-09-01 00:00:00
abstract::The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type:
doi:10.1002/term.3087
更新日期:2020-08-01 00:00:00
abstract::To spatially control therapeutic gene delivery for potential tissue engineering applications, a biotin-avidin interaction strategy was applied to immobilize viral vectors on biomaterial scaffolds. Both adenoviral vectors and gelatin sponges were biotinylated and avidin was applied to link them in a virus-biotin-avidin...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1774
更新日期:2016-02-01 00:00:00
abstract::Cartilage defects heal imperfectly and osteoarthritic changes develop frequently as a result. Although the existence of specific behaviours of chondrocytes derived from various depth-related zones in vitro has been known for over 20 years, only a relatively small body of in vitro studies has been performed with zonal ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1638
更新日期:2015-06-01 00:00:00
abstract::The gastroesophageal junction has been of clinical interest for some time due to its important role in preventing reflux of caustic stomach contents upward into the esophagus. Failure of this role has been identified as a key driver in gastroesophageal reflux disease, cancer of the lower esophagus, and aspiration-indu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.3045
更新日期:2020-06-01 00:00:00
abstract::Peripheral human nerves fail to regenerate across long tube implants (>2 cm), and tissue-engineered nerve grafts represent a promising treatment alternative. The present study aims to investigate the testosterone propionate (TP) repair effect of acellular nerve allograft (ANA) seeded with allogeneic bone marrow mesenc...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2922
更新日期:2019-09-01 00:00:00
abstract::Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered to be a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder its application. Few studies report the induction of ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2400
更新日期:2018-02-01 00:00:00
abstract::Organ transplantation is an effective treatment for chronic organ dysfunctioning conditions. However, a dearth of available donor organs for transplantation leads to the death of numerous patients waiting for a suitable organ donor. The potential of decellularized scaffolds, derived from native tissues or organs in th...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2061
更新日期:2017-04-01 00:00:00
abstract::The authors previously created HAp or CaCO(3) formed on or in agarose gels (HAp and CaCO(3) gels, respectively) as biocompatible and biodegradable bone graft materials. However, these gels have limitations for bone regeneration. Mesenchymal stromal cells (MSCs) have osteogenic potential and are considered useful for b...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.495
更新日期:2013-01-01 00:00:00
abstract::The effects of mesenchymal stem cells (MSCs) on the phenotype and function of natural killer T (NKT) cells is not understood. We used concanavalin A (Con A) and α-galactosylceramide (α-GalCer)-induced liver injury to evaluate the effects of MSCs on NKT-dependent hepatotoxicity. Mouse MSCs (mMSCs) significantly reduced...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2452
更新日期:2018-02-01 00:00:00
abstract::Nanopatterned (330 nm wide channels) type I collagen films were prepared by solvent casting on poly(dimethyl siloxane) (PDMS) templates. These films were rolled into tubular constructs and crosslinked. Tubular constructs were incubated under cell culture conditions for 28 days and examined by stereomicroscopy and scan...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.99
更新日期:2008-08-01 00:00:00
abstract::Tissue engineering holds great promise to develop functional constructs resembling the structural organization of native tissues to improve or replace biological functions, with the ultimate goal of avoiding organ transplantation. In tissue engineering, cells are often seeded into artificial structures capable of supp...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1978
更新日期:2016-09-01 00:00:00
abstract::Recellularization of ex vivo-derived scaffolds remains a significant hurdle primarily due to the scaffolds subcellular pore size that restricts initial cell seeding to the scaffolds periphery and inhibits migration over time. With the aim to improve cell migration, repopulation, and graft mechanics, the effects of a f...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3015
更新日期:2020-03-01 00:00:00
abstract::Intervertebral disc (IVD) degeneration has been described as an aberrant, cell-mediated, age- and genetics-dependent molecular degeneration process, which can be accelerated by nutritional, mechanical and toxic factors. Collective involvement of these factors can result in structural failures, which are often associat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1583
更新日期:2014-12-01 00:00:00
abstract::The self-regeneration capacity of articular cartilage is limited, due to its avascular and aneural nature. Loaded explants and cell cultures demonstrated that chondrocyte metabolism can be regulated via physiologic loading. However, the explicit ranges of mechanical stimuli that correspond to favourable metabolic resp...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.307
更新日期:2011-03-01 00:00:00
abstract::Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum techno...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1979
更新日期:2017-03-01 00:00:00