Abstract:
:A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull's-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull's-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull's-eye rot pathogens in port quarantine and orchard epidemiological studies.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Lin H,Jiang X,Yi J,Wang X,Zuo R,Jiang Z,Wang W,Zhou Edoi
10.1139/cjm-2017-0448subject
Has Abstractpub_date
2018-01-01 00:00:00pages
57-68issue
1eissn
0008-4166issn
1480-3275journal_volume
64pub_type
杂志文章abstract::The bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. The total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. Each compartment displays a specific flora, different from the flora...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-154
更新日期:1997-11-01 00:00:00
abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-164
更新日期:1980-08-01 00:00:00
abstract::Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-039
更新日期:1987-03-01 00:00:00
abstract::The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::We report a cinematographic analysis of culmination in fruiting bodies of Polysphondylium pallidum. During the final stages of aggregation, sorogen elongation is slow. An increase in the rate of sorogen elongation occurs when the growing stalk contacts the substratum, lifting the cell mass into the air. The elongation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-163
更新日期:1980-08-01 00:00:00
abstract::A field site near Humboldt, Saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. During each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-069
更新日期:2003-09-01 00:00:00
abstract::Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-083
更新日期:2005-11-01 00:00:00
abstract::The natural reservoirs and biological characteristics of pathogenic populations of both subspecies of Photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. In the present study, we assessed the occurrence of Photobacterium damselae sub...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-021
更新日期:2011-05-01 00:00:00
abstract::The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-057
更新日期:1994-05-01 00:00:00
abstract::Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-164
更新日期:1984-08-01 00:00:00
abstract::Photorhabdus temperata, an insect pathogen and nematode symbiont, is motile in liquid medium by swimming. We found that P. temperata was capable of surface movement, termed swarming behavior. Several lines of evidence indicate that P. temperata use the same flagella for both swimming and swarming motility. Both motili...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W10-119
更新日期:2011-03-01 00:00:00
abstract::High resolution two-dimensional PAGE was used to analyse protein variation among serotype 1 poliovirus isolates. Viruses isolated from patients with recent histories of vaccination with live attenuated poliovirus were compared with prototype serotype 1 poliovaccine. The nonvaccine Mahoney and Brunenders strains of ser...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-136
更新日期:1988-06-01 00:00:00
abstract::The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-078
更新日期:2006-12-01 00:00:00
abstract::Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-073
更新日期:1997-06-01 00:00:00
abstract::Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W06-132
更新日期:2007-02-01 00:00:00
abstract::The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0365
更新日期:2017-02-01 00:00:00
abstract::The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-183
更新日期:1995-01-01 00:00:00
abstract::Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0079
更新日期:2013-08-01 00:00:00
abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m95-091
更新日期:1995-08-01 00:00:00
abstract::Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-174
更新日期:1976-08-01 00:00:00
abstract::Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-303
更新日期:1975-12-01 00:00:00
abstract::A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-155
更新日期:1977-08-01 00:00:00
abstract::For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-047
更新日期:2003-05-01 00:00:00
abstract::Filamentous growth of Candida albicans occurs in response to a variety of environmental signals. The C. albicans gene orf19.1944 and its allele orf19.9499 are identical and are predicted to encode an 823-residue, 7-transmembrane-domain protein that has all the expected features of a G-protein-coupled receptor. The pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-095
更新日期:2004-12-01 00:00:00
abstract::Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-116
更新日期:2008-02-01 00:00:00
abstract::The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-080
更新日期:2001-09-01 00:00:00
abstract::A nystatin-resistant mutant of Aspergillus niger is described that has quantitative changes in phospholipid composition compared with the wild type. The proportion of linoleic acid in total phospholipids was elevated in the mutant from 38 to 56% and the proportion of oleic acid was lowered from 28 to 12%. Amino acid t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-076
更新日期:1990-06-01 00:00:00
abstract::The influence of plant growth promoting (PGP) activity of bacterial communities recovered from each of six weed species (barnyard grass (Echinochloa crusfalli (L.) Beauv.), corn spurrey (Spergula arvensis L.), goldenrod (Sonchus sp.), Italian ryegrass (Lolium multiflorum L.), lamb's-quarters (Chenopodium album L.), an...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-110
更新日期:2001-11-01 00:00:00
abstract::Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consorti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0323
更新日期:2017-01-01 00:00:00
abstract::Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00