Abstract：
:The ionic mechanism of the membrane permeability changes caused by L-glutamate in hippocampal neurons prepared from 17- to 19-day-old fetal rat in dispersed cell cultures was studied with the whole-cell variation of the patch electrode voltage-clamp technique. The cultured hippocampal neurons became sensitive to glutamate 7 days after plating, and thereafter the sensitivity gradually increased. The conductance increase caused by glutamate was voltage-sensitive, decreasing with membrane hyperpolarization at potentials more negative than -40 mV. The relative permeability of glutamate-activated channels to alkali metal and alkaline earth cations was estimated by reversal potential measurements. The alkali metal cations, Li+, Na+, K+, Rb+ and Cs+ were permeant to the glutamate channels, and the selectivity among them was weak. The alkaline earth cations, Ca2+, Sr2+ and Ba2+ were more permeant than the alkali metals. The permeability ratios of these divalent cations relative to Na+ were 2.4 (Ca2+), 2.4 (Sr2+) and 2.8 (Ba2+), respectively. Mg2+ was much less permeant and the permeability ratio (PMg/PNa) was only 0.1. Anion conductance made no contribution to the glutamate-induced current. Functional implications of the glutamate-induced increased in Ca2+-influx were discussed.
journal_name
Brain Resjournal_title
Brain researchauthors
Ozawa S,Nakamura T,Yuzaki Mdoi
10.1016/0006-8993(88)91601-0subject
Has Abstractpub_date
1988-03-08 00:00:00pages
85-94issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(88)91601-0journal_volume
443pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Opioid modulation of calcium currents was studied in acutely dissociated rat basal forebrain neurons using the whole cell patch-clamp recording technique. The mu-opioid receptor agonist DAGO reversibly suppressed high-voltage activated calcium currents and slowed their rate of activation, while neither delta- nor kapp...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00206-0
更新日期：1997-05-30 00:00:00
abstract：:Nuclear factor κB (NFκB) signaling plays ubiquitous roles in inflammation, immune response and neurogenesis. G protein-coupled receptor kinase 5 (GRK5) can protect neurons from degeneration. GRK5 also mediates tumor necrosis factor-α (TNFα)-induced NFκB signaling through the phosphorylation of IκBα. Here, we show that...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.02.041
更新日期：2015-05-22 00:00:00
abstract：:Evidence is presented for the occurrence of a unique opiate alkaloid-selective, opioid peptide-insensitive binding site in N18TG2 mouse neuroblastoma cells and in late passage hybrid F-11 cells, derived from N18TG2 neuroblastoma cells and rat dorsal root ganglion cells. Those cells lacked classical opioid peptide-sens...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91500-8
更新日期：1994-12-26 00:00:00
abstract：:The ability of crude cerebrum supernatant to stabilize, at a low temperature, cold-labile microtubules purified from beef brain was studied during development in the rat. The cold-stabilizing activity of the supernatant was low during the first postnatal week, rose significantly during the second postnatal week, and t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(88)90238-6
更新日期：1988-08-01 00:00:00
abstract：:The presence of thyrotropin-releasing-hormone-immunoreactive (TRH-ir) amacrine cells in the retina of amphibians is reported for the first time. The anuran and urodele retinas studied exhibit major differences in the distribution of TRH-ir cells. In the two urodele species investigated, most TRH-ir amacrine cells were...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03307-8
更新日期：2002-02-01 00:00:00
abstract：:The excitatory amino acid L-glutamate injected into the nucleus of the solitary tract (NTS) in unanesthetized rats similar to peripheral chemoreceptor activation increases mean arterial pressure (MAP) and reduces heart rate. In this study, we investigated the effects of acute (1 day) and chronic (15 days) electrolytic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.08.006
更新日期：2004-10-29 00:00:00
abstract：:The role of the central noradrenergic system in the supraoptic neuroendocrine regulation was investigated using slices of the guinea pig hypothalamus. Noradrenaline produced a complex membrane effect comprising two distinct depolarizations: one, associated with a moderate increase in input resistance and resulting in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91553-2
更新日期：1986-10-15 00:00:00
abstract：:The existence of spinal neuron collaterals projecting to the intralaminar thalamic nuclei (ILN) and the periaqueductal gray (PAG) was determined in the rat using double-labeling, fluorescent, retrograde axoplasmic transport techniques. Distinctively double-labeled neurons, although not numerous, were found in the enti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90731-6
更新日期：1986-02-12 00:00:00
abstract：:Intracerebral administration of 6-hydroxydopamine (6-HDA) along the dorsal noradrenergic bundle produced a gradual and long-lasting decrease of norepinephrine (NE) in hippocampus. This decrease in NE levels initially was accompanied by a parallel decrease in the activity of the rate-limiting enzyme for NE biosynthesis...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90056-8
更新日期：1984-12-24 00:00:00
abstract：:Platelet-derived growth factor-B (PDGF-B) is a growth factor that regulates cell migration, proliferation, and differentiation, and is involved in several physical and pathological processes. The overexpression of PDGF-B in glioma surgical samples revealed its effect on tumorigenesis. In this study, we determined that...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.01.045
更新日期：2014-03-27 00:00:00
abstract：:Immunohistochemical and neuronal tracing methods were used in cats to determine which type of postganglionic sympathetic neuron is innervated by preganglionic neurons which contain corticotrophin releasing factor-like immunoreactivity (CRF-LI). Preganglionic neurons with CRF-LI have their cell bodies at two restricted...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90399-t
更新日期：1992-12-25 00:00:00
abstract：:Collagen is one of the best materials used for nerve guide preparation due to its biocompatibility and desirable tensile strength. In this work, we have compared regeneration and functional reinnervation after sciatic nerve resection with bioresorbable crosslinked collagen guides in 10 mm gap. The crosslinking was car...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.03.022
更新日期：2005-06-07 00:00:00
abstract：:The density of specific prostaglandin E2 (PGE2) binding sites was quantitatively mapped in the rat brain using in vitro autoradiography. The anterior wall of the third ventricle and the nucleus solitary tract were found to have a very high density of binding sites (greater than 15 fmol/mg tissue). Two thalamic nuclei ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90720-t
更新日期：1992-05-29 00:00:00
abstract：:Recent studies have supported the existence of projections to the paraventricular and supraoptic nuclei of the hypothalamus that arise from non-catecholaminergic neurons in the nucleus of the solitary tract, whose terminal distribution is suggestive of interactions with both parvocellular and magnocellular neurosecret...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00760-n
更新日期：1995-10-02 00:00:00
abstract：:The ganglion cells are the output neurons of the retina. There is, however, relatively little known about the neurotransmitters used by these cells. In the present study, ganglion cells identified with a ganglion cell-specific monoclonal antibody (AB5) are shown in separate double-label experiments to be gamma-aminobu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91498-9
更新日期：1988-01-26 00:00:00
abstract：:The intergeniculate leaflet (IGL) is a distinct division of the lateral geniculate complex that participates in the regulation of the circadian rhythm through its projections to the circadian pacemaker located in the suprachiasmatic nuclei of the hypothalamus. A high number of neuropeptide Y (NPY) cell bodies has been...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)01032-4
更新日期：1998-11-30 00:00:00
abstract：:Alteration in the blood-nerve barrier may be of primary importance in the development of experimental lead neuropathy. Using quantitative track autoradiography, we have directly demonstrated that there is a 15-fold increase in the rate of 210Pb accumulation in the endoneurium 70 days after the onset of chronic intoxic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90318-8
更新日期：1981-11-23 00:00:00
abstract：:During normal aging, men experience a significant decline in testosterone levels and a compensatory elevation in levels of gonadotropin luteinizing hormone (LH). Both low testosterone and elevated LH have been identified as significant risk factors for the development of Alzheimer's disease (AD) in men. It is unclear ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.05.011
更新日期：2012-07-23 00:00:00
abstract：:Two neuropeptides reacting with antibodies directed against the C-terminal region of somatostatin 28(1-12) were purified to homogeneity from rat brain extracts. Amino acid analysis revealed that the larger peptide (8 kdaltons) consisted of 76 amino acids. Microsequencing established its aminoterminal structure as: Ala...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91394-5
更新日期：1984-10-08 00:00:00
abstract：:The recent indications of specialized lateralization of cardiovascular regulation within the right and left posterior insular cortex of the rat, suggest the possibility of transcallosal connectivity between these regions. This has not been previously demonstrated using physiological techniques. Extracellular neural re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02068-0
更新日期：2000-04-28 00:00:00
abstract：:Using a double-labeling method that combines retrograde tracing and immunocytochemistry, we demonstrated the presence of a hippocampofugal gamma-aminobutyric acid (GABA)-like immunoreactive (GABA-LI) neuron system in the rat. As a preliminary experiment, knife-cut of the dorsal fornix was made and it resulted in a rem...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90757-8
更新日期：1987-04-14 00:00:00
abstract：:The distribution of calretinin containing neurons examined by in situ hybridization mapping was compared with that obtained by immunocytochemistry in the brain of octopus. Results revealed a close correspondence between the two types of investigations. Western blot analysis disclosed a 29 kDa protein immunostained wit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.11.056
更新日期：2007-02-09 00:00:00
abstract：:In Parkinson disease (PD), the subthalamic nucleus (STN) becomes hyperactive (disinhibited), which is reported to cause excitotoxic damage to midbrain dopaminergic neurons. Here, we examined whether silencing of the hyperactive STN by chronic bilateral deep brain stimulation (DBS) increased the survival of midbrain do...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.08.082
更新日期：2006-11-20 00:00:00
abstract：:Tyrosine hydroxylase (TH) in freshly prepared 45,000 g supernatant from rat striatum was fractionated by DEAE-cellulose chromatography. The elution was made with 2 vols. of buffer (50 mM Tris, pH 7.4; 2 mM dithiothreitol) followed by 4 vols. of a linear NaCl gradient (0 0.3 M) in the same buffer. TH activity was elute...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90397-9
更新日期：1983-05-23 00:00:00
abstract：:Endogenous calcitonin was altered in rats by thyroparathyroidectomy, followed by supplementation with thyroxine and calcium. As a result, a reduction in the content of 5-hydroxytryptamine in the brain together with an increase in the concentration of tryptophan in the plasma were observed. The changes were accompanied...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90129-9
更新日期：1982-04-29 00:00:00
abstract：:Monoclonal antibody N210 (mabN210) recognizes the 210 kdalton neurofilament protein in mammals and gives characteristic immunocytochemical staining of neurofilament-rich processes. For example, in the cerebellum it recognizes myelinated axons and the calyx formed by basket cell axon collaterals. The distribution of ma...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90937-6
更新日期：1986-07-23 00:00:00
abstract：:Corticotropin-releasing factor (CRF) in the brain plays an important role in regulations of physiological and behavioral processes, yet CRF distribution in tree shrew brain has not been thoroughly and systematically reported. Here we examined the distribution of CRF immunoreactivity in the brain of tree shrews (Tupaia...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.06.004
更新日期：2015-08-27 00:00:00
abstract：:A gentle long-lasting handling produces persistent neurochemical and behavioural changes and attenuates the impairment in the behavioural reactivity to novelty induced by the prenatal exposure to diazepam (DZ) in adult male rat progeny. This study investigated the consequences of a late prenatal treatment with three G...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03282-1
更新日期：2002-10-25 00:00:00
abstract：:The regenerating optic nerve of goldfish first reestablishes a rough retinotopic map on the tectum, then goes through an activity dependent refinement that appears to involve the elimination of inappropriate branches from early regenerated arbors. Retinotopically appropriate branches and synapses may be stabilized bec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90886-4
更新日期：1988-04-19 00:00:00
abstract：:The dmy rat is an autosomal recessive mutant that exhibits severe myelin destruction throughout the white matter of the central nervous system. Recently, a point mutation in intron 3 of the Mrs2 has been found in the dmy rat. Mrs2 encodes an essential component of the major electrophoretic Mg(2+) influx system in mito...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.03.009
更新日期：2011-05-10 00:00:00
