Expression of the tumor suppressor gene hypermethylated in cancer 1 in laryngeal carcinoma.

Abstract:

:Hypermethylated in cancer 1 (HIC1) is a putative suppressor gene, cooperating with TP53 in the regulation of apoptosis. The promoter site of this gene contains CpG islands susceptible to methylation. Altered methylation leads to the silencing of HIC1. Persistent loss of HIC1 function reflects the attenuation of proapoptotic characteristics of TP53 and may constitute the background for carcinogenesis. Altered methylation profiles along with diminished expression of HIC1 were documented in a number of solid neoplasms. The aim of this study was to evaluate the expression of the HIC1 gene in laryngeal carcinoma. RNA was extracted from samples of laryngeal cancer and corresponding healthy tissues of 21 patients with advanced laryngeal cancer (T3-T4). The amount of RNA (cDNA) was evaluated using reverse transcription-quantitative polymerase chain reaction with GADPH as the reference gene. Data demonstrated that HIC1 expression was significantly reduced in laryngeal cancer tissues. The relative expression of HIC1 was found to be ~40% lower in tumor samples compared to that in healthy controls. The median tumor/normal tissue ratio for HIC1 was 0.615. These results suggest that low HIC1 expression may be associated with neoplastic transformation in the larynx.

journal_name

Oncol Lett

journal_title

Oncology letters

authors

Markowski J,Sieroń AL,Kasperczyk K,Ciupińska-Kajor M,Auguściak-Duma A,Likus W

doi

10.3892/ol.2015.2983

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

2299-2302

issue

5

eissn

1792-1074

issn

1792-1082

pii

OL-0-0-2983

journal_volume

9

pub_type

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