Expression of activation-induced cytidine deaminase splicing variants in patients with ankylosing spondylitis.

Abstract:

:To investigate the expression patterns of activation-induced cytidine deaminase (AID) variants in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and examine their clinical implications, we isolated PBMCs from healthy controls (HC, n = 33) and patients with AS (n = 62), and measured mRNA expression of AID variants and translesion synthesis (TLS) polymerases using quantitative real-time polymerase chain reaction. The proportion of patients with AS in whom AID splicing variant (sv) 2 was expressed was significantly higher than that of HC (p = .031). 80.7% of AS patients were treated with tumour necrosis factor inhibitor (TNFi). Significantly higher proportion of the TNFi-treated group expressed sv2 compared to the TNF-naïve group (p = .037). And we compared the level of AID variants expression between the TNFi-treated group and the TNF-naïve group. The expression levels of AID full-length (FL) and sv1 were significantly lower in the TNFi-treated group than the TNF-naïve group (FL: p = .002, sv1: p = .045). In addition, we investigated mRNA expression levels of translesion synthesis (TLS) polymerases in PBMCs from patients with AS and HC. The expression level of TLS pol ι was significantly lower in patients with AS than in HC (p = .007). In conclusion, AS patients expressed significantly higher levels of sv2 than HC. TNFi treatment restored the gene expression of the AID variants (FL, sv1, and sv2) in patients with AS. A clear understanding of the underlying cellular and molecular mechanisms will help to identify the pathogenesis of AS better and to develop novel therapeutic targets.

journal_name

Autoimmunity

journal_title

Autoimmunity

authors

Kim JY,Yoon HK,Song ST,Park SR,Shim SC

doi

10.1080/08916934.2017.1385777

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

435-440

issue

8

eissn

0891-6934

issn

1607-842X

journal_volume

50

pub_type

杂志文章
  • High circulating IL-6 level in Graves' ophthalmopathy.

    abstract::IL-6 is a paracrine and autocrine cytokine, which acts in the regulation of immunological and inflammatory processes. Its production can be observed in different cell types, as well as thyrocytes. The purpose of the study was to examine the serum IL-6 levels between the patients with Graves' disease (N = 47) and witho...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939708996275

    authors: Molnár I,Balázs C

    更新日期:1997-01-01 00:00:00

  • Aβ anti-idiotypic antibodies are present in intravenous immunoglobulin and are produced in mice following its administration.

    abstract::The effects of intravenous immunoglobulin (IVIG) products were recently examined in patients with Alzheimer's disease (AD). Although encouraging results were obtained in pilot studies, later trials produced negative results. The rationale for these studies was that IVIG contains antibodies to amyloid-beta (Aβ). Howeve...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916934.2014.983265

    authors: Loeffler DA,Klaver AC,Coffey MP

    更新日期:2015-06-01 00:00:00

  • B cells participate in tolerance and autoimmunity through cytokine production.

    abstract::In the 1950s, the discovery of autoantibodies produced by B cells seemed to provide a compelling mechanism underlying autoimmune diseases. The discovery of T regulatory cells and other T helper cell subsets shifted the field back towards a T cell central view. The success of rituxan, a chimeric mAb targeting CD20 on B...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.3109/08916934.2013.856006

    authors: Luu VP,Vazquez MI,Zlotnik A

    更新日期:2014-02-01 00:00:00

  • Mapping epitope specificities of monoclonal antibodies to thyroid peroxidase using recombinant antigen preparations.

    abstract::Five separate monoclonal antibodies (MoAbs) to human thyroid peroxidase (hTPO) were raised by immunising Balb/c mice with hTPO purified from detergent solubilised thyroid microsomes by high performance liquid chromatography (HPLC). The epitope specificities of these MoAbs were determined by assessing their ability to ...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939209035148

    authors: Ewins DL,Barnett PS,Tomlinson RW,McGregor AM,Banga JP

    更新日期:1992-01-01 00:00:00

  • Regulation and dysregulation of Epstein-Barr virus latency: implications for the development of autoimmune diseases.

    abstract::Epstein-Barr virus (EBV) is a human herpesvirus hiding in a latent form in memory B cells in the majority of the world population. Although, primary EBV infection is asymptomatic or causes a self-limiting disease, infectious mononucleosis, the virus is associated with a wide variety of neoplasms developing in immunosu...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1080/08916930802024772

    authors: Niller HH,Wolf H,Minarovits J

    更新日期:2008-05-01 00:00:00

  • Enhanced expression of heat shock protein gene in kidney lymphoid cells of lupus-prone mice during growing process.

    abstract::In the present study, the spontaneous elevation of the transcription of heat shock protein (hsp 70) gene in kidney lymphoid cells of lupus-prone mice (MRL-lpr/lpr) is shown by Northern blot and nuclear run on transcription assay. By quantification analysis of hsp 70 gene transcription, more than ten times of the enhan...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939108997141

    authors: Deguchi Y

    更新日期:1991-01-01 00:00:00

  • Treatment with anti-C5aR mAb leads to early-onset clinical and mechanistic effects in the murine delayed-type hypersensitivity arthritis model.

    abstract::Blockade of the complement cascade at the C5a/C5a receptor (C5aR)-axis is believed to be an attractive treatment avenue in rheumatoid arthritis (RA). However, the effects of such interventions during the early phases of arthritis remain to be clarified. In this study we use the murine delayed-type hypersensitivity art...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916934.2015.1031888

    authors: Atkinson SM,Nansen A,Usher PA,Sondergaard BC,Mackay CR,Friedrichsen B,Chang CC,Tang R,Skov S,Haase C,Hornum L

    更新日期:2015-01-01 00:00:00

  • Concentric fibrosis and cellular infiltration around bile ducts induced by graft-versus-host reaction in mice: a role of CD8+ cells.

    abstract::We report in this paper on obvious fibrotic lesions in the liver of mice undergoing specified graft-versus-host reaction (GVHR). B6 CD8+ splenocytes were transferred into (bm 12 x bm 1)F1 mice to induce GVHR. Recipient mice had been thymectomized and administrated with anti-CD8 monoclonal antibody (mAb) to deplete CD8...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939508995313

    authors: Inada S,Suzuki K,Kimura T,Hayashi A,Narita T,Yui R,Asakura H,Fujiwara M

    更新日期:1995-01-01 00:00:00

  • CTLA4Ig prevents initiation but not evolution of anti-phospholipid syndrome in NZW/BXSB mice.

    abstract::NZW "x" BXSB F1 mice develop SLE that is associated with an anti-phospholipid syndrome characterized by anti-cardiolipin antibodies, thrombocytopenia and small coronary artery thrombosis. This syndrome is immune mediated and, dependent, on CD4+T cells. To determine whether disease in these mice can be treated with blo...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.1080/08916930400008524

    authors: Akkerman A,Huang W,Wang X,Ramanujam M,Schiffer L,Madaio M,Factor SM,Davidson A

    更新日期:2004-09-01 00:00:00

  • Pathogenic mechanisms and experimental models of multiple sclerosis.

    abstract::Multiple sclerosis (MS) is a devastating autoimmune disease that affects more than 1 million people worldwide and severely compromises motor and sensory function through demyelination and axonal loss. This review covers current therapies, lessons learned from failed clinical trials, genetic susceptibility, key cell ty...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.3109/08916931003674733

    authors: Slavin A,Kelly-Modis L,Labadia M,Ryan K,Brown ML

    更新日期:2010-11-01 00:00:00

  • Polyarthritis in MRL-lpr/lpr mice: mouse type II collagen is antigenic but not arthritogenic.

    abstract::In addition to a lupus-like syndrome and massive T cell proliferation, MRL-lpr/lpr(MRL/l) mice develop an arthritic process very similar serologically and histologically to human rheumatoid arthritis (RA). Recently, we have developed in DBA/1 mice an experimental model of autoimmune arthritis (EAA) which shares clinic...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916938909034357

    authors: Boissier MC,Texier B,Carlioz A,Fournier C

    更新日期:1989-01-01 00:00:00

  • Role of CD5+ B-1 cells in EAE pathogenesis.

    abstract::Hybridoma cell lines producing natural autoantibodies (NAA), generated from A.SW mice with progressive experimental autoimmune encephalomyelitis (P-EAE), have been shown to cause demyelination and renal pathology when injected into naive mice. To investigate the relative contribution of these antibodies to disease pat...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.1080/08916930801890280

    authors: Peterson LK,Tsunoda I,Fujinami RS

    更新日期:2008-08-01 00:00:00

  • Common immunogenetic profile in children with multiple autoimmune diseases: the signature of HLA-DQ pleiotropic genes.

    abstract::Type 1 diabetes mellitus (T1DM), celiac disease (CD) and autoimmune thyroid disease (ATD) are autoimmune conditions relatively common in paediatric age and frequently occur in association in the same subject. This event is not by chance and requires an explanation. Here, we studied the distribution of HLA-DQ αβ hetero...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916934.2012.697594

    authors: Larizza D,Calcaterra V,Klersy C,Badulli C,Caramagna C,Ricci A,Brambilla P,Salvaneschi L,Martinetti M

    更新日期:2012-09-01 00:00:00

  • Factor XIII and tissue transglutaminase antibodies in coeliac and inflammatory bowel disease.

    abstract::Tissue transglutaminase (tTg) has been identified as an autoantigen in coeliac disease (CD). There is a marked homology between different forms of transglutaminase, such as tTg and coagulation factor XIII. We compared titres of both IgA- and IgG-antibodies against these two antigens in 20 CD patients, 20 endomysial an...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.1080/73-0891693021000005402

    authors: Sjöber K,Eriksson S,Tenngart B,Roth EB,Leffler H,Stenberg P

    更新日期:2002-08-01 00:00:00

  • The pathogenicity of spontaneously-occurring thyroglobulin-reactive T lymphocytes from BB/WOR rats.

    abstract::Autoantigen-reactive T lymphocytes have been implicated in the pathogenesis organ-specific autoimmune disease. Thyroglobulin (Tg) is one of the primary autoantigens associated with autoimmune lymphocytic thyroiditis (LT). These experiments investigated the pathogenicity of a lymphocyte line derived from spontaneously-...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939608995327

    authors: Allen EM,Thupari JN

    更新日期:1996-01-01 00:00:00

  • The origin of extracellular DNA during the clearance of dead and dying cells.

    abstract::DNA is a nuclear molecule that has both an intracellular and extracellular role. Inside the cell, it is the essential molecule of heredity while outside the cell it can have immunological activity, both alone and in the context of immune complexes. Furthermore, extracellular DNA has information content that can be min...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1080/08916930701358826

    authors: Pisetsky DS,Fairhurst AM

    更新日期:2007-06-01 00:00:00

  • Exposure of chromatin and not high affinity for dsDNA determines the nephritogenic impact of anti-dsDNA antibodies in (NZBxNZW)F1 mice.

    abstract::Recent studies have demonstrated that the nephritogenicity of antibodies to dsDNA and nucleosomes confers to binding of glomerular membrane-associated nucleosomes, and not to cross-reacting glomerular antigens. There is no known parameter that determines antibody pathogenicity aside from specificity for dsDNA/nucleoso...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.1080/08916930802375729

    authors: Mjelle JE,Kalaaji M,Rekvig OP

    更新日期:2009-02-01 00:00:00

  • Complement regulatory proteins and autoimmunity.

    abstract::The complement system is known to be involved in autoimmunity at several levels. Activated complement contributes to the inflammatory tissue injury characteristic of many autoimmune disease settings. On the other hand, early components of the classical pathway, including C1q, C4 and C2, are thought to be important for...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1080/08916930600739647

    authors: Song WC

    更新日期:2006-08-01 00:00:00

  • New developments in the pathogenesis of systemic sclerosis.

    abstract::Systemic sclerosis (SSc) is characterized by extensive fibrosis, vasculopathy and activation of the immune system. Fibrosis can be caused by profibrotic cytokines, such as transforming growth factor-beta (TGFbeta), interleukin-4 (IL-4), platelet-derived growth factor (PDGF), and connective tissue growth factor. Vascul...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1080/16066350500095415

    authors: Sakkas LI

    更新日期:2005-03-01 00:00:00

  • Expression of major histocompatibility complex class II antigen in NOD mouse thyroid.

    abstract::Non obese diabetic (NOD) mice spontaneously develop thyroiditis in addition to diabetes. Mononuclear cells begin to infiltrate the thyroid of these animals in the first month of life. The expression of major histocompatibility complex (MHC) class II (Ia) antigens by cells in the thyroid from NOD mice of various ages w...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939409014653

    authors: Margolese H,O'Keefe C,Chung F,Ertug F,Wall JR,Bernard NF

    更新日期:1994-01-01 00:00:00

  • Suppression of experimental autoimmune uveoretinitis by intraorchidic administration of S-antigen.

    abstract::Experimental autoimmune uveoretinitis (EAU) induced by immunization of albino Lewis rats with a retinal soluble antigen (S-antigen) has been studied extensively by many workers. An intraorchidic injection of S-antigen 4 days prior to immunization of the animal with the antigen emulsified in adjuvant was found to preve...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939209083134

    authors: Peng B,Yoshitoshi T,Shichi H

    更新日期:1992-01-01 00:00:00

  • Efficacy and toxicity of treatments for nephritis in a series of consecutive lupus patients.

    abstract:OBJECTIVE:To study efficacy and toxicity of treatments for nephritis in a series of consecutive lupus patients. METHODS:The case records of 40 patients with lupus nephritis followed up in a single center between 1992 and 2011 (median duration = 8.37 years) were retrieved to determine efficacy and toxicity of the treat...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916934.2013.817560

    authors: Bozzolo EP,Ramirez GA,Bonavida G,Lanzani C,Scotti R,Dell'antonio G,Baldissera E,Canti V,Manfredi AA,Rovere-Querini P,Sabbadini MG

    更新日期:2013-12-01 00:00:00

  • Pemphigus autoimmunity: hypotheses and realities.

    abstract::The goal of contemporary research in pemphigus vulgaris and pemphigus foliaceus is to achieve and maintain clinical remission without corticosteroids. Recent advances of knowledge on pemphigus autoimmunity scrutinize old dogmas, resolve controversies, and open novel perspectives for treatment. Elucidation of intimate ...

    journal_title:Autoimmunity

    pub_type: 杂志文章,评审

    doi:10.3109/08916934.2011.606444

    authors: Grando SA

    更新日期:2012-02-01 00:00:00

  • A 63 kDa skeletal muscle protein associated with eye muscle inflammation in Graves' disease is identified as the calcium binding protein calsequestrin.

    abstract::It is generally accepted that thyroid-associated ophthalmopathy (TAO) is an autoimmune disease of the eye muscle (EM) and the surrounding orbital connective tissue in which circulating antibodies play an important role. Antibodies against EM membrane proteins of 63-67kDa mol. wt. seem to be the best markers of ophthal...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939908995967

    authors: Gunji K,Kubota S,Stolarski C,Wengrowicz S,Kennerdell JS,Wall JR

    更新日期:1999-01-01 00:00:00

  • Distinct surface phenotypes of B cells responsible for spontaneous production of IgM and IgG anti-DNA antibodies in autoimmune-prone NZB x NZW F1 mice.

    abstract::Autoimmune-prone NZB x NZW F1 (B/W F1) mice produce a high titer of anti-DNA antibodies, In vivo and in vitro studies showed that in the early life of these mice, the immunoglobulin isotype of these antibodies almost exclusively belongs to IgM class, however, IgG anti-DNA antibodies begin to develop when the mice are ...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939008993383

    authors: Okada T,Abe M,Takiura F,Hirose S,Shirai T

    更新日期:1990-01-01 00:00:00

  • Inhibition of autoimmune disease by the immunomodulator linomide correlates with the ability to activate macrophages.

    abstract::Linomide is a potent immunomodulator that has been shown to inhibit autoimmunity in several animal models of autoimmune disease, including experimental autoimmune encephalomyelitis (EAE). Linomide's mechanism of action is unknown, however, it has been suggested to modulate the function of antigen presenting cells (APC...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916930008994093

    authors: Dahlén E,Andersson M,Dawe K,Tellander AC,Brunmark C,Björk A,Hedlund G

    更新日期:2000-10-01 00:00:00

  • Celiac antibodies in children with type 1 diabetes - A diagnostic validation study.

    abstract:INTRODUCTION:Autoimmune diseases, such as celiac disease (CD) and diabetes mellitus type 1, tend to co-occur within the same patient. The prevalence of CD in diabetic children is higher than in the general population, and is estimated to be 0.6-16.4%. The diagnosis of CD is based on histopathological examination and se...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.1080/08916934.2018.1427226

    authors: Lewandowska K,Ciepiela O,Szypowska A,Wyhowski J,Głodkowska-Mrówka E,Popko K,Ostafin M,Pyrżak B,Demkow U

    更新日期:2018-03-01 00:00:00

  • Reduced in vitro production of interferon-gamma, interleukin-4 and interleukin-12 and increased production of interleukin-6, interleukin-10 and tumour necrosis factor-alpha in systemic lupus erythematosus. Weak correlations of cytokine production with dis

    abstract::Production of cytokines in unstimulated and mitogen-stimulated cultures were evaluated by ELISPOT in 34 SLE patients with low to moderate disease activity and 23 healthy controls. Significantly reduced production of IFN gamma, IL4 and IL12 and significantly increased production of IL6, IL10 and TNF alpha were found in...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939908994055

    authors: Jones BM,Liu T,Wong RW

    更新日期:1999-10-01 00:00:00

  • Studies with recombinant autoepitopes of thyroid peroxidase: evidence suggesting an epitope shared between the thyroid and the gastric parietal cell.

    abstract::In a previous work we characterized a major epitope of thyroid peroxidase (TPO), which was recognised by 66% of 157 patients with autoimmune thyroid disease (AITD) and 9 out of 50 patients with non-thyroidal autoimmune disease (NTAID) 6 of whom had antibodies to the gastric parietal cell antigen (PCA). In the present ...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.3109/08916939008998434

    authors: Elisei R,Mariotti S,Swillens S,Vassart G,Ludgate M

    更新日期:1990-01-01 00:00:00

  • Functional and isokinetic assessment of muscle strength in patients with idiopathic inflammatory myopathies.

    abstract:OBJECTIVE:To assess muscle strength in patients with idiopathic inflammatory myopathies (IIM) using neuromuscular scales and isokinetic testing. METHODS:Muscle function was evaluated in 27 IIM patients being followed at the Rheumatology Unit of the University of Pisa using: (i) a modified version of the grading system...

    journal_title:Autoimmunity

    pub_type: 杂志文章

    doi:10.1080/08916930600623817

    authors: Neri R,Mosca M,Stampacchia G,Vesprini E,Tavoni A,d'Ascanio A,Bombardieri S

    更新日期:2006-05-01 00:00:00