Abstract:
:For the reliable urinary measurement of calcium, phosphate and uric acid, a pre-analytical process by adding acid or base to urine samples at laboratory is recommended in order to dissolve precipitated solutes. Several studies on different kind of samples and analysers have previously shown that a such pre-analytical treatment is useless. The objective was to study the necessity of pre-analytical treatment of urine on samples collected using the V-Monovette® (Sarstedt) system and measured on the analyser Architect C16000 (Abbott Diagnostics). Sixty urinary samples of hospitalized patients were selected (n=30 for calcium and phosphate, and n=30 for uric acid). After acidification of urine samples for measurement of calcium and phosphate, and alkalinisation for measurement of uric acid respectively, differences between results before and after the pre-analytical treatment were compared to acceptable limits recommended by the French society of clinical biology (SFBC). No difference in concentration between before and after pre-analytical treatment of urine samples exceeded acceptable limits from SFBC for measurement of calcium and uric acid. For phosphate, only one sample exceeded these acceptable limits, showing a result paradoxically lower after acidification. In conclusion, in agreement with previous study, our results show that acidification or alkalinisation of urine samples from 24 h urines or from urination is not a pre-analytical necessity for measurement of calcium, phosphate and uric acid.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Petit M,Beaudeux JL,Majoux S,Hennequin Cdoi
10.1684/abc.2017.1271subject
Has Abstractpub_date
2017-10-01 00:00:00pages
519-524issue
5eissn
0003-3898issn
1950-6112pii
abc.2017.1271journal_volume
75pub_type
杂志文章abstract::The introduction of the CRM 470 in 1993 (certified reference material for 14 serum proteins) and its utilization by industrial companies for cross-calibrating their commercial standards has been an important break-through in protein standardization. This improvement has been clearly illustrated by the last national qu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Bacillus alvei hemolytic toxin has been characterized in five main steps from a 18 hours culture supernatant fluid. 50% ammonium sulfate precipitations are followed by Sephadex gel filtrations. After that we proceed to an isoelectric focusing in a narrow pH gradient (from 4 to 6). A final step consists in a gel filtra...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Apolipoprotein B (apoB) is the major proteic component of LDL, VLDL and chylomicrons. Numerous polymorphisms of the apolipoprotein B gene have been described. Particularly, a polymorphism of insertion/deletion located in the coding part of the signal peptide of apoB, associated with modifications of lipid concentratio...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::A group of genetic disorders of the red cell, appearing as congenital haemolytic anaemias, were first described on morphological bases, due mainly to the presence of stomatocytes. It turned out, however, that the alterations of the shape could widely vary, and even be absent, thereby questionning the classification on...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1999-07-01 00:00:00
abstract::Twenty four strains of coagulase-negative staphylococci from clinically significant blood cultures were separated in four groups on the basis of oxacillin susceptibility and phenotype of resistance to aminoglycoside antibiotics. Susceptibility testing included disk diffusion testing, M.I.C. and M.B.C. determinations a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Thermal burns result in severe electrolytes disturbances which are life-threatening when the percentage of burnt body surface area (BSA) is above 20% in adults and 10% in children. If electrolytes disturbances are often mentioned in the physiopathology of burns, they are less documented in the daily practice of the fo...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::Nowadays, physiological amino acids profiling is based primarily on ion exchange chromatography (IEC) coupled to a post-column derivatization with ninhydrin and UV detection at two wavelengths. Unfortunately, this technique suffers various drawbacks such as long analysis time, high sample volume and specific costs rel...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2015.1066
更新日期:2015-07-01 00:00:00
abstract::The stimulation regimens and the results of ovulation induction in anovulatory patients and in patients suffering from a Luteinized Unruptured Follicle (LUF) syndrome are discussed as well as the findings concerning superovulation in IVF cycles. The percentage of multiple pregnancies (less than or equal to 20 p. cent)...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Anti-citrullinated cyclic peptide antibodies (ACPA) were initially considered very specific for the diagnosis of rheumatoid arthritis (RA), and can predict the prognosis of the disease. However, these antibodies can be detected in other autoimmune diseases, including systemic lupus erythematosus (SLE), the most common...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2020.1518
更新日期:2020-04-01 00:00:00
abstract::The indirect fluorescent antibody (IFA) technique for specific IgM anti-Candida albicans was applied to 158 sera and compared with sucrose density gradient centrifugation. For detection of specific IgM results of both methods were in agreement for 157 sera (99.36%). Specific IgM titers determined with IFA on unfractio...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::http://biotribune.com/ Biotribune.com ou la biologie medicale sur internet. Ce site est concu comme un portail sur la biologie clinique. Accueil d'internautes sur une page comportant, entre autres, les rubriques : actualite, evenements, revue des connaissances, rubrique juridique et petites annonces. Biotribune propos...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::We have compared three assays for serum galactosyltransferase activity, which use ovomucoïd, asialo agalactofetuin and free N-acetylglucosamine respectively as exogenous acceptors. A very good correlation between the three assays is obtained, for the whole range of GT activity. When the methods are compared to one ano...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The discriminatory potency of variable number of tandem repeats (VNTR), based on 7 loci (MIRU 26, 27 and 5 ETRs A, B, C, D, E) was assayed on Mycobacterium bovis strains obtained from samples due to tuberculosis in two slaughterhouses in Algeria. The technique of MIRU-VNTR has been evaluated on 88 strains of M. bovis ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2010.0459
更新日期:2010-07-01 00:00:00
abstract::A diffuse lesion in the alveolar-capillary membrane appears as an initial pathological event which preludes the emergence of an Acute Respiratory Distress (ARDS) with pulmonary oedema. The implication of polymorphous nuclear cells (PMN) as the cells initiating this lesion remains solidly established. The activation of...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three ho...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::In this report, the authors have studied the plasma and red blood cell concentrations of both zinc and copper in 90 uremic patients treated by intermittent hemodialysis. They found that plasmatic and erythrocytic levels of zinc together with the copper red blood cell concentrations determined before and after the hemo...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1989-01-01 00:00:00
abstract::French medical laboratories must be accredited before November 2016 according to NF/EN/ISO 15189 standard. However, technical accreditation guidelines cannot be applied literally for the determination of specific IgE for several reasons: more than 600 allergen tests, lack of international gold standard, limited extern...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0830
更新日期:2013-05-01 00:00:00
abstract::This article gives a greed for evaluation of the application in medical laboratory according to requirements of the standard EN ISO 15189 : 2012. This is to allow the responsible of the laboratory to check the requirements of this standard to be applied. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0847
更新日期:2013-06-01 00:00:00
abstract::Therapeutic monitoring of patients participating in methadone maintenance programmes requires the qualitative or quantitative determination of methadone in urine before and during treatment. Generally immunoassay techniques are used for this determination, but more and more often measurement of plasma or serum levels ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::The authors describe a method of fractionation of urinary 17-oxosteroids after purification of the urinary extract by Girard's T reagent. After solvolysis, enzyme hydrolysis and purification by Girard's T reagent, the 17-oxosteroids were estimated in the form of trimethyl silyl ethers by gas liquid chromatographye on ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Activation of plasminogen by tissue-type plasminogen activator (tpA) is potentiated by fibrin. We have demonstrated the role of fibrin polymerization in the potentiating effect of tpA-induced fibrinolysis. Therefore a pathogenic mechanism of thrombotic disorder may be related to an abnormal fibrin polymerization: the ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The GSH-400 method (Oxis International SA) performed in acid supernatant, provides an assay of reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine) (GSH) in biological samples by a two step chemical reaction followed by a spectrophotometric detection. We improved the analytical performances of the assay by using ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::This document is a proposal of questionnaire for a self-assessment of the post-examination phase: results validation, reporting and transmitting, post-examination samples keeping and documents archival storage. The questions allow to check that the laboratory fulfils the ISO 15189 Standard and COFRAC SH REF 02 documen...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2012.0675
更新日期:2012-02-01 00:00:00
abstract::The analytical modifications necessary for adaptation of the SFBC technic for measurement of the activity of TGO method and those obtained by the technic adapted on ABA 100 suggest that the modifications proposed have no influence on the result of the enzyme estimation. The activation of TGO by addition of its coenzym...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::This paper points out the structural and physiological data of galectin-3, and emphasizes its role in cardiac fibrosis and heart failure pathophysiology. Then we summarize the optimal conditions for sampling, assays and we discuss the interpretation of results. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2014.0977
更新日期:2014-07-01 00:00:00
abstract::Twenty-two amino acids are measured in cerebrospinal fluid of fourty patients with senile dementia of the Alzheimer type (SDAT) and of ten age matched healthy control subjects. There is no significant difference between these two groups. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Blood histamine proved to be important for the immune homeostasis. By its direct action or by means of H-2 receptors it end or prevents the excessive immune response. Recent studies revealed changes in cell mediated immunity in subjects with atopia, with increased histaminemia and their obvious protection against canc...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1990-01-01 00:00:00
abstract::This review of the immunological diagnosis of parasitic diseases defines the various indications, the means of collection and preparation, the various levels of specificity and the choice of parasitic antigen which should be used for immuno-diagnosis. The detection and assay of circulating antibodies relies on the tec...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1983-01-01 00:00:00
abstract::Serum digoxin measurement is often performed in medical laboratories. A professional association specialized in quality control, based in Lyon, has been organizing punctual controls of medication measurement for the past ten years. The results are analysed in term of intra and inter-technique precision, difference bet...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::Determination of sex-hormone-binding-globulin was performed by three methods: binding, electroimmunodiffusion and DELFIA methods. Results of normal and pathological values correlated well with the three techniques. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00