Clinical Usefulness of Immunohistochemical Staining of p57 kip2 for the Differential Diagnosis of Complete Mole.

Abstract:

OBJECTIVE:Can polymer-based immunohistochemical staining of p57(kip2) replace DNA analysis as an inexpensive means of differentiating complete mole from partial mole or hydropic abortion? METHODS AND MATERIALS:Original paraffin-embedded tissue blocks from 14 equivocal cases were turned over to our laboratory and examined by immunohistochemical staining of p57(kip2). RESULTS:Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining. The remaining 10 cases showed apparently positive staining in cytotrophoblasts and villous stromal cells. Without DNA analysis we are able to clearly differentiate the 4 cases of complete mole among the 14 equivocal cases. During follow-up, secondary low-risk gestational trophoblastic neoplasia (GTN) developed in 1 of the 4 cases of complete mole: the GTN was treated by single-agent chemotherapy. No subsequent changes were observed during follow-up in the other cases. CONCLUSION:Polymer-based immunohistochemical staining of p57(kip2) (paternally imprinted gene, expressed from maternal allele) is a very effective method that can be used to differentiate androgenetic complete mole from partial mole and hydropic abortion. We might be able to avoid the cost of DNA analysis.

journal_name

Biomed Res Int

authors

Sasaki S,Sasaki Y,Kunimura T,Sekizawa A,Kojima Y,Iino K

doi

10.1155/2015/905648

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

905648

eissn

2314-6133

issn

2314-6141

journal_volume

2015

pub_type

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