Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro.

Abstract:

:Haploid pluripotent stem cells, such as haploid embryonic stem cells (haESCs), facilitate the genetic study of recessive traits. In vitro, fish haESCs maintain haploidy in both undifferentiated and differentiated states, but whether mammalian haESCs can preserve pluripotency in the haploid state has not been tested. Here, we report that mouse haESCs can differentiate in vitro into haploid epiblast stem cells (haEpiSCs), which maintain an intact haploid genome, unlimited self-renewal potential, and durable pluripotency to differentiate into various tissues in vitro and in vivo. Mechanistically, the maintenance of self-renewal potential depends on the Activin/bFGF pathway. We further show that haEpiSCs can differentiate in vitro into haploid progenitor-like cells. When injected into the cytoplasm of an oocyte, androgenetic haEpiSC (ahaEpiSCs) can support embryonic development until midgestation (E12.5). Together, these results demonstrate durable pluripotency in mouse haESCs and haEpiSCs, as well as the valuable potential of using these haploid pluripotent stem cells in high-throughput genetic screening.

journal_name

J Mol Cell Biol

authors

Shuai L,Wang Y,Dong M,Wang X,Sang L,Wang M,Wan H,Luo G,Gu T,Yuan Y,Feng C,Teng F,Li W,Liu X,Li T,Wang L,Wang XJ,Zhao XY,Zhou Q

doi

10.1093/jmcb/mjv044

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

326-37

issue

4

eissn

1674-2788

issn

1759-4685

pii

mjv044

journal_volume

7

pub_type

杂志文章
  • NSD3S stabilizes MYC through hindering its interaction with FBXW7.

    abstract::The MYC transcription factor plays a key role in cell growth control. Enhanced MYC protein stability has been found to promote tumorigenesis. Thus, understanding how MYC stability is controlled may have significant implications for revealing MYC-driven growth regulatory mechanisms in physiological and pathological pro...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjz098

    authors: Gonzalez-Pecchi V,Kwan AK,Doyle S,Ivanov AA,Du Y,Fu H

    更新日期:2020-07-03 00:00:00

  • Proteome-wide Data Analysis Reveals Tissue-specific Network Associated with SARS-CoV-2 Infection.

    abstract::For patients with COVID-19 caused by SARS-CoV-2, the damages to multiple organs have been clinically observed. Since most of current investigations for virus-host interaction are based on cell level, there is an urgent demand to probe tissue-specific features associated with SARS-CoV-2 infection. Based on collected pr...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjaa033

    authors: Feng L,Yin YY,Liu CH,Xu KR,Li QR,Wu JR,Zeng R

    更新日期:2020-07-08 00:00:00

  • The GYF domain protein CD2BP2 is critical for embryogenesis and podocyte function.

    abstract::Scaffolding proteins play pivotal roles in the assembly of macromolecular machines such as the spliceosome. The adaptor protein CD2BP2, originally identified as a binding partner of the adhesion molecule CD2, is a pre-spliceosomal assembly factor that utilizes its glycine-tyrosine-phenylalanine (GYF) domain to co-loca...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjv039

    authors: Albert GI,Schell C,Kirschner KM,Schäfer S,Naumann R,Müller A,Kretz O,Kuropka B,Girbig M,Hübner N,Krause E,Scholz H,Huber TB,Knobeloch KP,Freund C

    更新日期:2015-10-01 00:00:00

  • Molecular mechanisms of ligand-mediated attenuation of DNA binding by MarR family transcriptional regulators.

    abstract::Bacteria and archaea encode members of the large multiple antibiotic resistance regulator (MarR) family of transcriptional regulators. Generally, MarR homologs regulate activity of genes involved in antibiotic resistance, stress responses, virulence or catabolism of aromatic compounds. They constitute a diverse group ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjq021

    authors: Perera IC,Grove A

    更新日期:2010-10-01 00:00:00

  • Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.

    abstract::Motile cilia and flagella are microtubule-based organelles important for cell locomotion and extracellular liquid flow through beating. Although axonenal dyneins that drive ciliary beat have been extensively studied in unicellular Chlamydomonas, to what extent such knowledge can be applied to vertebrate is poorly know...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjy043

    authors: Zhang Y,Chen Y,Zheng J,Wang J,Duan S,Zhang W,Yan X,Zhu X

    更新日期:2019-05-01 00:00:00

  • microRNA-129-5p, a c-Myc negative target, affects hepatocellular carcinoma progression by blocking the Warburg effect.

    abstract::Deregulation of microRNAs (miRNAs) and c-Myc (Myc) contributes to hepatocellular carcinoma (HCC) progression, but how miRNAs and Myc regulate each other in hepatocarcinogenesis is still poorly understood. Using a functional screen, we identified miR-129-5p as a miRNA that inhibits HCC cell growth. miR-129-5p targets t...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjw010

    authors: Han H,Li W,Shen H,Zhang J,Zhu Y,Li Y

    更新日期:2016-10-01 00:00:00

  • Paired related homeobox 1 transactivates dopamine D2 receptor to maintain propagation and tumorigenicity of glioma-initiating cells.

    abstract::Glioblastoma multiforme (GBM) is a highly invasive brain tumor with limited therapeutic means and poor prognosis. Recent studies indicate that glioma-initiating cells/glioma stem cells (GICs/GSCs) may be responsible for tumor initiation, infiltration, and recurrence. GICs could aberrantly employ molecular machinery ba...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjx017

    authors: Li Y,Wang W,Wang F,Wu Q,Li W,Zhong X,Tian K,Zeng T,Gao L,Liu Y,Li S,Jiang X,Du G,Zhou Y

    更新日期:2017-08-01 00:00:00

  • Drugging in the absence of p53.

    abstract::Inactivation of the p53 gene is a key driver of tumorigenesis in various cancer cohorts and types. The quest for a successful p53-based therapy that holds the promise of treating more than half of the cancer population has culminated in extensive knowledge about the role and function of p53 and led to new proposed inn...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章,评审

    doi:10.1093/jmcb/mjz012

    authors: Aning OA,Cheok CF

    更新日期:2019-03-01 00:00:00

  • Bidirectional regulation of fragile X mental retardation protein phosphorylation controls rhodopsin homoeostasis.

    abstract::Homoeostatic regulation of the light sensor, rhodopsin, is critical for the maintenance of light sensitivity and survival of photoreceptors. The major fly rhodopsin, Rh1, undergoes light-induced endocytosis and degradation, but its protein and mRNA levels remain constant during light/dark cycles. It is not clear how t...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjw041

    authors: Wang X,Mu Y,Sun M,Han J

    更新日期:2017-04-01 00:00:00

  • Rheb promotes brown fat thermogenesis by Notch-dependent activation of the PKA signaling pathway.

    abstract::Increasing brown and beige fat thermogenesis have an anti-obesity effect and thus great metabolic benefits. However, the molecular mechanisms regulating brown and beige fat thermogenesis remain to be further elucidated. We recently found that fat-specific knockout of Rheb promoted beige fat thermogenesis. In the curre...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjz056

    authors: Meng W,Liang X,Xiao T,Wang J,Wen J,Luo H,Teng J,Fei Y,Zhang Q,Liu B,Hu F,Bai J,Liu M,Zhou Z,Liu F

    更新日期:2019-09-19 00:00:00

  • E-selectin ligand complexes adopt an extended high-affinity conformation.

    abstract::E-selectin is a cell-adhesion molecule of the vascular endothelium that promotes essential leukocyte rolling in the early inflammatory response by binding to glycoproteins containing the tetrasaccharide sialyl Lewis(x) (sLe(x)). Efficient leukocyte recruitment under vascular flow conditions depends on an increased lif...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjv046

    authors: Preston RC,Jakob RP,Binder FP,Sager CP,Ernst B,Maier T

    更新日期:2016-02-01 00:00:00

  • The long and the short of it: the MDM4 tail so far.

    abstract::The mouse double minute 4 (MDM4) is emerging from the shadow of its more famous relative MDM2 and is starting to steal the limelight, largely due to its therapeutic possibilities. MDM4 is a vital regulator of the tumor suppressor p53. It restricts p53 transcriptional activity and also, at least in development, facilit...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章,评审

    doi:10.1093/jmcb/mjz007

    authors: Haupt S,Mejía-Hernández JO,Vijayakumaran R,Keam SP,Haupt Y

    更新日期:2019-03-01 00:00:00

  • NKT cell self-reactivity: evolutionary master key of immune homeostasis?

    abstract::Complex immune responses have evolved to protect multicellular organisms against the invasion of pathogens. This has exerted strong developmental pressure for specialized functions that can also limit damage to self-tissue. Two arms of immunity, the innate and adaptive immune systems, have evolved for quick, non-speci...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章,评审

    doi:10.1093/jmcb/mjr035

    authors: Issazadeh-Navikas S

    更新日期:2012-04-01 00:00:00

  • A role for paralog-specific sumoylation in histone deacetylase 1 stability.

    abstract::Histone deacetylase 1 (HDAC1) is an essential epigenetic regulator belonging to a highly conserved family of deacetylases. Increased HDAC1 activity and expression often correlates with neoplastic transformation. Here we show how specific modification of HDAC1 by SUMO1, but not by SUMO2, facilitates HDAC1 degradation. ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjt032

    authors: Citro S,Jaffray E,Hay RT,Seiser C,Chiocca S

    更新日期:2013-12-01 00:00:00

  • Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling.

    abstract::Our earlier studies have demonstrated that the cigarette smoke in the form of cigarette smoke condensate (CSC) causes growth arrest of a mouse spermatocyte cell line [GC-2spd(ts)] through activation of the AHR-NRF2 pathway. The present study demonstrates the CSC-activated p38 and ERK MAPK signaling in GC-2spd(ts) via ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mju049

    authors: Esakky P,Hansen DA,Drury AM,Moley KH

    更新日期:2015-02-01 00:00:00

  • FGF8 signaling sustains progenitor status and multipotency of cranial neural crest-derived mesenchymal cells in vivo and in vitro.

    abstract::The cranial neural crest (CNC) cells play a vital role in craniofacial development and regeneration. They are multi-potent progenitors, being able to differentiate into various types of tissues. Both pre-migratory and post-migratory CNC cells are plastic, taking on diverse fates by responding to different inductive si...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjv052

    authors: Shao M,Liu C,Song Y,Ye W,He W,Yuan G,Gu S,Lin C,Ma L,Zhang Y,Tian W,Hu T,Chen Y

    更新日期:2015-10-01 00:00:00

  • Role of the lncRNA-p53 regulatory network in cancer.

    abstract::Advances in functional genomics have led to discovery of a large group of previous uncharacterized long non-coding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs may serve as master gene regulators through various mechanisms. Dysregulation of lncRNAs is often associated with a variety of human diseases inclu...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章,评审

    doi:10.1093/jmcb/mju013

    authors: Zhang A,Xu M,Mo YY

    更新日期:2014-06-01 00:00:00

  • Myeloid adrenergic signaling via CaMKII forms a feedforward loop of catecholamine biosynthesis.

    abstract::Type 2 immune response has been shown to facilitate cold-induced thermogenesis and browning of white fat. However, whether alternatively activated macrophages produce catecholamine and substantially promote adaptive thermogenesis in adipose tissue remains controversial. Here, we show that tyrosine hydroxylase (TyrH), ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjx046

    authors: Luo Y,Liu B,Yang X,Ma X,Zhang X,Bragin DE,Yang XO,Huang W,Liu M

    更新日期:2017-10-01 00:00:00

  • Cancer and neurodegenerative disorders: pathogenic convergence through microRNA regulation.

    abstract::Although cancer and neurodegenerative disease are two distinct pathological disorders, emerging evidence indicates that these two types of disease share common mechanisms of genetic and molecular abnormalities. Recent studies show that individual microRNAs (miRNAs) could be involved in the pathology of both diseases, ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章,评审

    doi:10.1093/jmcb/mjq058

    authors: Du L,Pertsemlidis A

    更新日期:2011-06-01 00:00:00

  • Wnt1-cre-mediated conditional loss of Dicer results in malformation of the midbrain and cerebellum and failure of neural crest and dopaminergic differentiation in mice.

    abstract::The involvement of microRNAs (miRNAs) in the development of the neural crest (NC) cells and other neuronal differentiation is still poorly understood. Here, we investigated the global function of miRNAs in embryonic development by examining the Wnt1-cre-mediated Dicer knockout mice. Dicer ablation resulted in malforma...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjq008

    authors: Huang T,Liu Y,Huang M,Zhao X,Cheng L

    更新日期:2010-06-01 00:00:00

  • H3K9me2 attracts PGC7 in the zygote to prevent Tet3-mediated oxidation of 5-methylcytosine.

    abstract::Epigenetic reprogramming of the parental genome occurs in fertilized oocytes and involves oxidation of 5-methylcytosines (5-mC) to 5-hydroxymethylcytosines (5-hmC) in the paternal pronucleus. Recent work has shown that the maternal genome is protected from this remodeling step by an interaction between a modified hist...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjs038

    authors: Szabó PE,Pfeifer GP

    更新日期:2012-12-01 00:00:00

  • Molecular suicide notes: last call from apoptosing cells.

    abstract::Dying cells actively attract professional phagocytes by sending out soluble 'find-me' signals. The characterization and identification of the corresponding molecular entities have revealed that 'find-me' signals come in different flavors, including peptides, proteins, lipids and complex structures, such as apoptotic m...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjp045

    authors: Peter C,Wesselborg S,Lauber K

    更新日期:2010-04-01 00:00:00

  • Do substantia nigra dopaminergic neurons differentiate between reward and punishment?

    abstract::The activity of dopaminergic neurons are thought to be increased by stimuli that predict reward and decreased by stimuli that predict aversive outcomes. Recent work by Matsumoto and Hikosaka challenges this model by asserting that stimuli associated with either rewarding or aversive outcomes increase the activity of d...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjp010

    authors: Frank MJ,Surmeier DJ

    更新日期:2009-10-01 00:00:00

  • Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling.

    abstract::Adipocyte is the most predominant cell type in the tumor microenvironment of breast cancer and plays a pivotal role in cancer progression, yet the underlying mechanisms and functional mediators remain elusive. We isolated primary preadipocytes from mammary fat pads of human breast cancer patients and generated mature ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjaa016

    authors: Liu L,Wu Y,Zhang C,Zhou C,Li Y,Zeng Y,Zhang C,Li R,Luo D,Wang L,Zhang L,Tu S,Deng H,Luo S,Chen YG,Xiong X,Yan X

    更新日期:2020-09-01 00:00:00

  • SP1 governs primordial folliculogenesis by regulating pregranulosa cell development in mice.

    abstract::Establishment of the primordial follicle (PF) pool is pivotal for the female reproductive lifespan; however, the mechanism of primordial folliculogenesis is poorly understood. Here, the transcription factor SP1 was shown to be essential for PF formation in mice. Our results showed that SP1 is present in both oocytes a...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjz059

    authors: Cai H,Liu B,Wang H,Sun G,Feng L,Chen Z,Zhou J,Zhang J,Zhang T,He M,Yang T,Guo Q,Teng Z,Xin Q,Zhou B,Zhang H,Xia G,Wang C

    更新日期:2020-04-24 00:00:00

  • AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells.

    abstract::X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression. In female mammals, mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript (XIST) is essential for initiation of X chromosome inactivation upon differ...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjy074

    authors: Zhang Y,Wang C,Liu X,Yang Q,Ji H,Yang M,Xu M,Zhou Y,Xie W,Luo Z,Lin C

    更新日期:2019-09-19 00:00:00

  • New march towards the regeneration of sensation and cognition: hear more, see more and learn more.

    abstract::As many human sensory and cognitive diseases are caused by irreversible damage or loss of certain types of neurons, methodologies aimed at replacement of lost neurons are key to restore lost sensation. Recent advances in generation of ear-cell progenitors, optic-cup structures and cortical neurons from human embryonic...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章,评审

    doi:10.1093/jmcb/mjs060

    authors: Zhang K,Yi F,Liu GH,Belmonte JC

    更新日期:2013-04-01 00:00:00

  • The crossregulation between ERK and PI3K signaling pathways determines the tumoricidal efficacy of MEK inhibitor.

    abstract::MEK inhibitor has been highlighted as a promising anti-tumor drug but its effect has been reported as varying over a wide range depending on patho-physiological conditions. In this study, we employed a systems approach by combining biochemical experimentation with in silico simulations to investigate the resistance me...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjs021

    authors: Won JK,Yang HW,Shin SY,Lee JH,Heo WD,Cho KH

    更新日期:2012-06-01 00:00:00

  • Proteomic analyses of the SMYD family interactomes identify HSP90 as a novel target for SMYD2.

    abstract::The SMYD (SET and MYND domain) family of lysine methyltransferases (KMTs) plays pivotal roles in various cellular processes, including gene expression regulation and DNA damage response. Initially identified as genuine histone methyltransferases, specific members of this family have recently been shown to methylate no...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjr025

    authors: Abu-Farha M,Lanouette S,Elisma F,Tremblay V,Butson J,Figeys D,Couture JF

    更新日期:2011-10-01 00:00:00

  • Toward precision CRISPR DNA fragment editing and predictable 3D genome engineering.

    abstract::Ever since gene targeting or specific modification of genome sequences in mice was achieved in the early 1980s, the reverse genetic approach of precise editing of any genomic locus has greatly accelerated biomedical research and biotechnology development. In particular, the recent development of the CRISPR/Cas9 system...

    journal_title:Journal of molecular cell biology

    pub_type: 杂志文章

    doi:10.1093/jmcb/mjaa060

    authors: Wu Q,Shou J

    更新日期:2020-10-30 00:00:00