Abstract:
:Haploid pluripotent stem cells, such as haploid embryonic stem cells (haESCs), facilitate the genetic study of recessive traits. In vitro, fish haESCs maintain haploidy in both undifferentiated and differentiated states, but whether mammalian haESCs can preserve pluripotency in the haploid state has not been tested. Here, we report that mouse haESCs can differentiate in vitro into haploid epiblast stem cells (haEpiSCs), which maintain an intact haploid genome, unlimited self-renewal potential, and durable pluripotency to differentiate into various tissues in vitro and in vivo. Mechanistically, the maintenance of self-renewal potential depends on the Activin/bFGF pathway. We further show that haEpiSCs can differentiate in vitro into haploid progenitor-like cells. When injected into the cytoplasm of an oocyte, androgenetic haEpiSC (ahaEpiSCs) can support embryonic development until midgestation (E12.5). Together, these results demonstrate durable pluripotency in mouse haESCs and haEpiSCs, as well as the valuable potential of using these haploid pluripotent stem cells in high-throughput genetic screening.
journal_name
J Mol Cell Bioljournal_title
Journal of molecular cell biologyauthors
Shuai L,Wang Y,Dong M,Wang X,Sang L,Wang M,Wan H,Luo G,Gu T,Yuan Y,Feng C,Teng F,Li W,Liu X,Li T,Wang L,Wang XJ,Zhao XY,Zhou Qdoi
10.1093/jmcb/mjv044subject
Has Abstractpub_date
2015-08-01 00:00:00pages
326-37issue
4eissn
1674-2788issn
1759-4685pii
mjv044journal_volume
7pub_type
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